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Centers for Disease Control and Prevention. 2014 Druid Hills, GA, USA http:\/\/www.cdc.gov\/vhf\/ebola\/healthcare-us\/cleaning\/hospitals.html<\/ext-link>,"},{"issue":"3","key":"ref6","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1093\/infdis\/jiw254","article-title":"Ebola Virus Shedding and Transmission: Review of Current Evidence","volume":"214","author":"P Vetter","year":"2016","journal-title":"Journal of Infec Dis"},{"issue":"3","key":"ref7","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1021\/acs.estlett.5b00029","article-title":"Inactivation of an enveloped surrogate virus in human sewage","volume":"2","author":"LM Casanova","year":"2015","journal-title":"Environmental Science & Technology Letters"},{"issue":"9","key":"ref8","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1021\/acs.estlett.5b00193","article-title":"Persistence of Ebola Virus in sterilized wastewater","volume":"2","author":"K Bibby","year":"2015","journal-title":"Environmental Science & Technology Letters"},{"issue":"5","key":"ref9","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1001\/jama.1997.03550050061035","article-title":"Clinical recognition and management of patients exposed to biological warfare agents","volume":"278","author":"DR Franz","year":"1997","journal-title":"Journal of the American Medical Association"},{"key":"ref10","unstructured":"Sterk E. Filovirus Haemorrhagic Fever Guideline. M\u00e9decins Sans Fronti\u00e8res. 2008. www.medbox.org\/filovirus-haemorrhagic-fever\u2026\/download.pdf<\/ext-link>."},{"key":"ref11","unstructured":"Ebola virus disease: Key questions and answers concerning water, sanitation and hygiene World Health Organization \/ UNICEF. 2014.; http:\/\/www.who.int\/csr\/resources\/publications\/ebola\/water-sanitation-hygiene\/en\/<\/ext-link>."},{"key":"ref12","unstructured":"Interim Recommendations for Cleaning Houses Safely in West Africa Ebola-Affected Areas after Persons with Symptoms of Ebola are transferred to Ebola Treatment Units or Community Care Centers. Centers for Disease Control and Prevention.2015 Druid Hills, GA, USA. http:\/\/www.cdc.gov\/vhf\/ebola\/hcp\/cleaning-houses-safely-in-west-africa.html<\/ext-link>."},{"key":"ref13","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1086\/423182","article-title":"Disinfection and sterilization in health care facilities: what clinicians need to know","volume":"39","author":"WA Rutala","year":"2004","journal-title":"Clin Infec Dis"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1975","DOI":"10.3390\/v7041975","article-title":"Evaluating Environmental Persistence and Disinfection of the Ebola Virus Makona Variant","volume":"7","author":"B Cook","year":"2015","journal-title":"Viruses"},{"issue":"36","key":"ref15","first-page":"1","article-title":"Chlorination of drinking water in emergencies: a review of knowledge to develop recommendations for implementation and research needed","author":"A Branz","year":"2017","journal-title":"Waterlines"},{"issue":"7","key":"ref16","doi-asserted-by":"crossref","first-page":"1557","DOI":"10.1016\/S0043-1354(02)00522-5","article-title":"Disinfection efficacy of organic chloramines","volume":"37","author":"MM Donnermair","year":"2003","journal-title":"Water Res"},{"issue":"1","key":"ref17","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1128\/AEM.42.1.159-167.1981","article-title":"Effect of turbidity on chlorination efficiency and bacterial persistence in drinking water","volume":"42","author":"MW. Lechevallier","year":"1981","journal-title":"App Environ Microb"},{"issue":"2","key":"ref18","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0005299","article-title":"Disinfection of Ebola Virus in Sterilized Municipal Wastewater","volume":"11","author":"K. Bibby","year":"2017","journal-title":"Plos Neglect Trop D"},{"key":"ref19","unstructured":"Trajano DGS, Dias E, Ebdon J, Taylor H. Limitations of chlorine disinfection of human excreta: Implications for Ebola disease control. 39th WEDC International Conference, Kumasi, Ghana, 2016. http:\/\/wedc.lboro.ac.uk\/resources\/conference\/39\/Trajano-2446.pdf<\/ext-link>"},{"key":"ref20","unstructured":"WHO Report: Ebola Virus Disease (EVD) Key questions and answers concerning water, sanitation and hygiene [Internet]. World Health Organization; 2014b pp. 1\u20135. http:\/\/www.who.int\/water_sanitation_health\/WASH_and_Ebola.pdf<\/ext-link>"},{"key":"ref21","doi-asserted-by":"crossref","DOI":"10.1201\/9781482273489","article-title":"Biosolids treatment and management: processes for beneficial use","author":"MJ Girovich","year":"1996"},{"issue":"12","key":"ref22","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1016\/0043-1354(69)90077-3","article-title":"The bactericidal effect of lime flocculation\/flotation as a primary unit process in a multiple system for the advanced purification of sewage works effluent","volume":"3","author":"WOK Grabow","year":"1969","journal-title":"Water Res"},{"issue":"4","key":"ref23","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1128\/AEM.35.4.663-669.1978","article-title":"Role of lime treatment in the removal of bacteria, enteric viruses, and coliphages in a wastewater reclamation plant","volume":"35","author":"WOK Grabow","year":"1978","journal-title":"App Environ Microbiol"},{"issue":"2","key":"ref24","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/S1093-0191(02)00009-6","article-title":"High-pH magnesium coagulation-flocculation in wastewater treatment","volume":"7","author":"L Semerjian","year":"2003","journal-title":"Adv Environ Res"},{"issue":"3","key":"ref25","doi-asserted-by":"crossref","first-page":"79","DOI":"10.2166\/wst.1989.0082","article-title":"Wastewater treatment for effluent reuse\u2014Lime-induced removal of excreted pathogens","volume":"21","author":"MP Gambrill","year":"1989","journal-title":"Water Sci Technol"},{"key":"ref26","article-title":"Research Monographs in Tropical Public Health Engineering","volume":"3","author":"HD Taylor","year":"1994"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"247","DOI":"10.2166\/wst.1994.0620","article-title":"Upgrading a low-cost physicochemical wastewater treatment plant to solve operational problems","volume":"29","author":"HD Taylor","year":"1994","journal-title":"Water Sci Technol"},{"key":"ref28","unstructured":"Morrison, SM, Martin, KL, Humble, DE, Lime disinfection of sewage bacteria at low temperature. 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Torodel","year":"2010"},{"issue":"22","key":"ref39","doi-asserted-by":"crossref","first-page":"2054","DOI":"10.1056\/NEJMp1413084","article-title":"Ebola Virus Disease in West Africa\u2014Clinical Manifestations and Management","volume":"371","author":"DS Chertow","year":"2014","journal-title":"New Engl J Med"},{"key":"ref40","unstructured":"Anonymous. ISO 9308\u20131:2000. Water Quality. Detection and enumeration of Escherichia coli and coliform bacteria Part 1: Membrane filtration method. International Organization for Standardization, Geneva, Switzerland, 2000."},{"key":"ref41","unstructured":"Anonymous. ISO 7899\u20132:2000. Water quality: Detection and enumeration of intestinal enterococci Part 2: Membrane filtration method. International Organization for Standardization, Geneva, Switzerland, 2000."},{"key":"ref42","unstructured":"Anonymous. ISO 10705\u20132. Water quality\u2014Detection and enumeration of bacteriophages\u2014Part 2: enumeration of somatic coliphages. International Organisation for Standardization, Geneva, Switzerland, 2001"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1128\/AEM.52.3.430-433.1986","article-title":"Practical direct plaque-assay for coliphages in 100-ml samples of drinking-water","volume":"52","author":"WOK Grabow","year":"1986","journal-title":"App Environ Microbiol"},{"key":"ref44","unstructured":"Anonymous. ISO 10705\u20131. Water quality\u2014Detection and enumeration of bacteriophages\u2014Part 1: enumeration of F-specific RNA bacteriophages. International Organisation for Standardization, Geneva, Switzerland, 2001."},{"key":"ref45","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1111\/j.1365-2672.1984.tb01372.x","article-title":"A method for the enumeration of male-specific bacteriophages in sewage","volume":"56","author":"AH Havelaar","year":"1984","journal-title":"J App Bacteriol"},{"key":"ref46","unstructured":"Anonymous. ISO 10705\u20134. Water quality\u2014Detection and enumeration of bacteriophages\u2014Part 4: enumeration of bacteriophages infecting Bacteroides fragilis. International Organisation for Standardization. Geneva, Switzerland, 2001."},{"key":"ref47","doi-asserted-by":"crossref","first-page":"5659","DOI":"10.1128\/AEM.71.9.5659-5662.2005","article-title":"Method for isolation of Bacteroides bacteriophage host strains suitable for tracking sources of fecal pollution in water","volume":"71","author":"A Payan","year":"2005","journal-title":"App Environ Microbiol"},{"key":"ref48","unstructured":"APHA\/AWWA\/WEF\u2014Standard methods for the examination of water and wastewater American Public Health Association \/ American Water Works Association \/ Water Environment Federation. 22nd ed. Washington DC; 2012"},{"issue":"5","key":"ref49","doi-asserted-by":"crossref","first-page":"e0152442","DOI":"10.1371\/journal.pone.0152442","article-title":"Accuracy, Precision, Ease-Of-Use, and Cost of Methods to Test Ebola-Relevant Chlorine Solutions","volume":"11","author":"E Wells","year":"2016","journal-title":"PLoS ONE"},{"issue":"5","key":"ref50","doi-asserted-by":"crossref","first-page":"e0156136","DOI":"10.1371\/journal.pone.0156136","article-title":"Shelf-Life of Chlorine Solutions Recommended in Ebola Virus Disease Response","volume":"11","author":"Q Iqbal","year":"2016","journal-title":"PLoS ONE"},{"issue":"3","key":"ref51","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1128\/AEM.54.3.649-654.1988","article-title":"Factors Promoting Survival Of Bacteria In Chlorinated Water-Supplies","volume":"54","author":"MW Lechevallier","year":"1988","journal-title":"Applied and Environmental Microbiology"},{"key":"ref52","unstructured":"Gensch R, Jennings A, Renggli S, Reymond P. (2018). Compendium of Sanitation Technologies in Emergencies\u20141st Edition. German WASH Network, Swiss Federal Institute of Aquatic Science and Technology, Global WASH Cluster and Sustainable Sanitation Alliance. 1st ed. Berlin, Germany, 2018."},{"issue":"4","key":"ref53","doi-asserted-by":"crossref","first-page":"2038","DOI":"10.1128\/AEM.69.4.2038-2043.2003","article-title":"Chlorination of indicator bacteria and viruses in primary sewage effluent","volume":"69","author":"JA Tree","year":"2003","journal-title":"App and Environ Microbiol"},{"issue":"9","key":"ref54","doi-asserted-by":"crossref","first-page":"3227","DOI":"10.1128\/AEM.61.9.3227-3231.1995","article-title":"Potential Usefulness Of Bacteriophages That Infect Bacteroides-Fragilis As Model Organisms For Monitoring Virus Removal In Drinking-Water Treatment Plants","volume":"61","author":"J Jofre","year":"1995","journal-title":"Applied and Environmental Microbiology"},{"issue":"1","key":"ref55","doi-asserted-by":"crossref","first-page":"47","DOI":"10.20506\/rst.14.1.829","article-title":"Modes of action of disinfectants","volume":"14","author":"P Maris","year":"1995","journal-title":"Revue Scientifique Et Technique De L Office International Des Epizooties"},{"issue":"8","key":"ref56","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1016\/0043-1354(77)90114-2","article-title":"Mechanism of Disinfection\u2014Effect of Chlorine on cell-membrane functions","volume":"11","author":"C Venkobachar","year":"1977","journal-title":"Water Res"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1016\/j.jenvman.2016.08.026","article-title":"Bactericidal and virucidal mechanisms in the alkaline disinfection of compost using calcium lime and ash","volume":"181","author":"N Hijikata","year":"2016","journal-title":"J Environ Managem"},{"issue":"21","key":"ref58","doi-asserted-by":"crossref","first-page":"12069","DOI":"10.1021\/es3029473","article-title":"Virus Inactivation Mechanisms: Impact of Disinfectants on Virus Function and Structural Integrity","volume":"46","author":"KR. Wigginton","year":"2012","journal-title":"Environ Sci Technol"},{"issue":"5","key":"ref59","doi-asserted-by":"crossref","first-page":"e0177943","DOI":"10.1371\/journal.pone.0177943","article-title":"Selection of a Biosafety Level 1 (BSL-1) surrogate to evaluate surface disinfection efficacy in Ebola outbreaks: Comparison of four bacteriophages","volume":"12","author":"K Gallandat","year":"2017","journal-title":"Plos One"},{"issue":"15","key":"ref60","doi-asserted-by":"crossref","first-page":"8692","DOI":"10.1021\/acs.est.7b01296","article-title":"Evaluation of Phi6 Persistence and Suitability as an Enveloped Virus Surrogate","volume":"51","author":"N Aquino de Carvalho","year":"2017","journal-title":"Environ Sci Technol"},{"issue":"12","key":"ref61","doi-asserted-by":"crossref","first-page":"4658","DOI":"10.1128\/AEM.64.12.4658-4662.1998","article-title":"Effects of starvation on physiological activity and chlorine disinfection resistance in Escherichia coli O157: H7","volume":"64","author":"J T. 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