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Mekonnen","year":"2017","journal-title":"Parasites & Vectors"},{"key":"ref84","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1110\/ps.051745906","article-title":"A general model of G protein-coupled receptor sequences and its application to detect remote homologs","volume":"15","author":"M Wistrand","year":"2006","journal-title":"Protein Science: A Publication of the Protein Society"},{"key":"ref85","doi-asserted-by":"crossref","first-page":"W362","DOI":"10.1093\/nar\/gkl124","article-title":"ScanProsite: detection of PROSITE signature matches and ProRule-associated functional and structural residues in proteins","volume":"34","author":"E de Castro","year":"2006","journal-title":"Nucleic Acids Research"},{"key":"ref86","doi-asserted-by":"crossref","first-page":"3981","DOI":"10.1021\/bi00066a019","article-title":"Purification, cloning, and cofactor independence of glutamate racemase from Lactobacillus","volume":"32","author":"KA Gallo","year":"1993","journal-title":"Biochemistry"},{"key":"ref87","doi-asserted-by":"crossref","first-page":"2796","DOI":"10.1099\/mic.0.2008\/020933-0","article-title":"Moonlighting function of glutamate racemase from Mycobacterium tuberculosis: racemization and DNA gyrase inhibition are two independent activities of the enzyme","volume":"154","author":"S Sengupta","year":"2008","journal-title":"Microbiology"},{"key":"ref88","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1111\/j.1751-7915.2008.00031.x","article-title":"Glutamate racemase as a target for drug discovery","volume":"1","author":"SL Fisher","year":"2008","journal-title":"Microb Biotechnol"},{"key":"ref89","doi-asserted-by":"crossref","first-page":"S167","DOI":"10.1038\/sj.bjp.0707662","article-title":"G-protein-coupled receptor phosphorylation: where, when and by whom","volume":"153","author":"AB Tobin","year":"2008","journal-title":"British Journal of Pharmacology"},{"key":"ref90","unstructured":"WormBase (Not stated) Gene \u00bb T04F8.2"},{"key":"ref91","unstructured":"WHO WHO (2015,) APOC statement on the 2015 annual letter of the Bill & Melinda Gates Foundation."},{"key":"ref92","doi-asserted-by":"crossref","first-page":"e0005330","DOI":"10.1371\/journal.pntd.0005330","article-title":"Identification of three immunodominant motifs with atypical isotype profile scattered over the Onchocerca volvulus proteome","volume":"11","author":"O Lagatie","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"key":"ref93","doi-asserted-by":"crossref","first-page":"44","DOI":"10.4103\/2229-5070.113907","article-title":"Immunodiagnosis of fascioliasis using sandwich enzyme-linked immunosorbent assay for detection of Fasciola gigantica paramyosin antigen","volume":"3","author":"HMA Abou-Elhakam","year":"2013","journal-title":"Tropical Parasitology"},{"key":"ref94","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1128\/CDLI.8.3.571-578.2001","article-title":"Use of synthetic peptides derived 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Clinical Immunology"},{"key":"ref98","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1111\/j.1365-2249.1994.tb06548.x","article-title":"Ivermectin-facilitated immunity in onchocerciasis; activation of parasite-specific Th1-type responses with subclinical Onchocerca volvulus infection","volume":"96","author":"PT Soboslay","year":"1994","journal-title":"Clin Exp Immunol"},{"key":"ref99","doi-asserted-by":"crossref","first-page":"1567","DOI":"10.1093\/infdis\/172.6.1567","article-title":"Differential decline in filaria-specific IgG1, IgG4, and IgE antibodies in Brugia malayi-infected patients after diethylcarbamazine chemotherapy","volume":"172","author":"AK Atmadja","year":"1995","journal-title":"J Infect Dis"}],"container-title":["PLOS 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