{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T06:57:59Z","timestamp":1790146679337,"version":"4.0.1"},"reference-count":46,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2018,10,11]],"date-time":"2018-10-11T00:00:00Z","timestamp":1539216000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Agence Nationale de la recherche","award":["ANR-13-BSV8-0002-01"],"award-info":[{"award-number":["ANR-13-BSV8-0002-01"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0204861","type":"journal-article","created":{"date-parts":[[2018,10,11]],"date-time":"2018-10-11T17:38:17Z","timestamp":1539279497000},"page":"e0204861","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Distinct virulence ranges for infection of mice by Bordetella pertussis revealed by engineering of the sensor-kinase BvgS"],"prefix":"10.1371","volume":"13","author":[{"given":"Elodie","family":"Lesne","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8626-8109","authenticated-orcid":true,"given":"Loic","family":"Coutte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"Solans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"Slupek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne-Sophie","family":"Debrie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2698-5955","authenticated-orcid":true,"given":"V\u00e9ronique","family":"Dhennin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Froguel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Hot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camille","family":"Locht","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudy","family":"Antoine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5102-1704","authenticated-orcid":true,"given":"Fran\u00e7oise","family":"Jacob-Dubuisson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2018,10,11]]},"reference":[{"issue":"Suppl 1","key":"ref1","first-page":"74","article-title":"Pertussis disease and transmission and host responses: insights from the baboon model of pertussis","author":"MV Pinto","year":"2017","journal-title":"J Infect"},{"issue":"1","key":"ref2","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/S1369-5274(00)00169-7","article-title":"<italic>Bordetella pertussis<\/italic>, molecular pathogenesis under multiple aspects","volume":"4","author":"C Locht","year":"2001","journal-title":"Curr Opin Microbiol"},{"key":"ref3","first-page":"47","article-title":"Bordetella Molecular Biology Norfolk","author":"S. Stibitz","year":"2007"},{"issue":"3","key":"ref4","doi-asserted-by":"crossref","first-page":"700","DOI":"10.1111\/j.1365-2958.2005.04875.x","article-title":"Role of BvgA phosphorylation and DNA binding affinity in control of Bvg-mediated phenotypic phase transition in <italic>Bordetella pertussis<\/italic>","volume":"58","author":"AM Jones","year":"2005","journal-title":"Mol Microbiol"},{"issue":"10","key":"ref5","doi-asserted-by":"crossref","first-page":"5537","DOI":"10.1128\/IAI.00781-06","article-title":"Growth phase- and nutrient limitation-associated transcript abundance regulation in <italic>Bordetella pertussis<\/italic>","volume":"74","author":"MM Nakamura","year":"2006","journal-title":"Infect Immun"},{"issue":"3","key":"ref6","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1046\/j.1365-2958.2001.02415.x","article-title":"Diversity in the <italic>Bordetella<\/italic> virulence regulon: transcriptional control of a Bvg-intermediate phase gene","volume":"40","author":"R Deora","year":"2001","journal-title":"Mol Microbiol"},{"issue":"12","key":"ref7","doi-asserted-by":"crossref","first-page":"3971","DOI":"10.1002\/j.1460-2075.1991.tb04967.x","article-title":"Sequential activation and environmental regulation of virulence genes in <italic>Bordetella pertussis<\/italic>","volume":"10","author":"V Scarlato","year":"1991","journal-title":"EMBO J"},{"issue":"3","key":"ref8","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1111\/j.1365-2958.2004.04418.x","article-title":"Demonstration of differential virulence gene promoter activation in vivo in <italic>Bordetella pertussis<\/italic> using RIVET","volume":"55","author":"WL Veal-Carr","year":"2005","journal-title":"Mol Microbiol"},{"issue":"4","key":"ref9","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1046\/j.1365-2958.1997.3821741.x","article-title":"A mutation in the <italic>Bordetella bronchiseptica bvgS<\/italic> gene results in reduced virulence and increased resistance to starvation, and identifies a new class of Bvg-regulated antigens","volume":"24","author":"PA Cotter","year":"1997","journal-title":"Mol Microbiol"},{"issue":"24","key":"ref10","doi-asserted-by":"crossref","first-page":"6942","DOI":"10.1128\/JB.184.24.6942-6951.2002","article-title":"Differential regulation of the <italic>Bordetella bipA<\/italic> gene: distinct roles for different BvgA binding sites","volume":"184","author":"R. Deora","year":"2002","journal-title":"J Bacteriol"},{"issue":"52","key":"ref11","doi-asserted-by":"crossref","first-page":"33176","DOI":"10.1074\/jbc.271.52.33176","article-title":"Central role of the BvgS receiver as a phosphorylated intermediate in a complex two-component phosphorelay","volume":"271","author":"MA Uhl","year":"1996","journal-title":"J Biol Chem"},{"issue":"3","key":"ref12","doi-asserted-by":"crossref","first-page":"e1004700","DOI":"10.1371\/journal.ppat.1004700","article-title":"Virulence Regulation with Venus Flytrap Domains: Structure and Function of the Periplasmic Moiety of the Sensor-Kinase BvgS","volume":"11","author":"E Dupre","year":"2015","journal-title":"PLoS Pathog"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"23307","DOI":"10.1074\/jbc.M115.655720","article-title":"Signal Transduction by BvgS Sensor-Kinase: Binding of Modulator Nicotinate Affects Conformation and Dynamics of Entire Periplasmic Moiety","volume":"290","author":"E Dupre","year":"2015","journal-title":"J Biol Chem"},{"key":"ref14","first-page":"57","article-title":"Two-component system in bacteria","author":"F Jacob-Dubuisson","year":"2012"},{"issue":"40","key":"ref15","doi-asserted-by":"crossref","first-page":"17351","DOI":"10.1073\/pnas.1006267107","article-title":"Periplasmic domain of the sensor-kinase BvgS reveals a new paradigm for the Venus flytrap mechanism","volume":"107","author":"J Herrou","year":"2010","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"1","key":"ref16","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1111\/j.1365-2958.1996.tb02484.x","article-title":"Atomic structure and specificity of bacterial periplasmic receptors for active transport and chemotaxis: variation of common themes","volume":"20","author":"FA Quiocho","year":"1996","journal-title":"Mol Microbiol"},{"issue":"15","key":"ref17","doi-asserted-by":"crossref","first-page":"11348","DOI":"10.1016\/S0021-9258(18)82131-7","article-title":"Three-dimensional structures of the periplasmic lysine\/arginine\/ornithine-binding protein with and without a ligand","volume":"268","author":"BH Oh","year":"1993","journal-title":"J Biol Chem"},{"issue":"2","key":"ref18","doi-asserted-by":"crossref","first-page":"e02089","DOI":"10.1128\/mBio.02089-15","article-title":"Balance between Coiled-Coil Stability and Dynamics Regulates Activity of BvgS Sensor Kinase in Bordetella","volume":"7","author":"E Lesne","year":"2016","journal-title":"MBio"},{"issue":"18","key":"ref19","doi-asserted-by":"crossref","first-page":"e00114","DOI":"10.1128\/JB.00114-17","article-title":"Conformational changes of inter-domain linker mediate mechanical signal transmission in sensor-kinase BvgS","volume":"199","author":"E Lesne","year":"2017","journal-title":"J Bacteriol"},{"issue":"1","key":"ref20","doi-asserted-by":"crossref","first-page":"e02052","DOI":"10.1128\/mBio.02052-17","article-title":"Coiled-coil antagonism regulates activity of Venus flytrap-domain-containing sensor-kinases of the BvgS family","volume":"9","author":"E Lesne","year":"2018","journal-title":"mBIO"},{"issue":"14","key":"ref21","doi-asserted-by":"crossref","first-page":"e140","DOI":"10.1093\/nar\/gkt444","article-title":"Computational analysis of bacterial RNA-Seq data","volume":"41","author":"R McClure","year":"2013","journal-title":"Nucleic Acids Res"},{"issue":"9","key":"ref22","doi-asserted-by":"crossref","first-page":"e6996","DOI":"10.1371\/journal.pone.0006996","article-title":"Molecular evolution of the two-component system BvgAS involved in virulence regulation in <italic>Bordetella<\/italic>","volume":"4","author":"J Herrou","year":"2009","journal-title":"PLoS One"},{"issue":"5","key":"ref23","doi-asserted-by":"crossref","first-page":"1775","DOI":"10.1128\/JB.188.5.1775-1785.2006","article-title":"Species- and strain-specific control of a complex, flexible regulon by <italic>Bordetella<\/italic> BvgAS","volume":"188","author":"CA Cummings","year":"2006","journal-title":"J Bacteriol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"32774","DOI":"10.1038\/srep32774","article-title":"The multifaceted RisA regulon of <italic>Bordetella pertussis<\/italic>","volume":"6","author":"L Coutte","year":"2016","journal-title":"Sci Rep"},{"issue":"5","key":"ref25","doi-asserted-by":"crossref","first-page":"e01526","DOI":"10.1128\/mBio.01526-17","article-title":"The BvgAS Regulon of <italic>Bordetella pertussis<\/italic>","volume":"8","author":"K Moon","year":"2017","journal-title":"MBio"},{"issue":"2","key":"ref26","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1128\/IAI.73.2.748-760.2005","article-title":"Evaluation of the role of the Bvg intermediate phase in <italic>Bordetella pertussis<\/italic> during experimental respiratory infection","volume":"73","author":"N Vergara-Irigaray","year":"2005","journal-title":"Infect Immun"},{"issue":"3","key":"ref27","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1128\/IAI.06016-11","article-title":"Phenotypic modulation of the virulent Bvg phase is not required for pathogenesis and transmission of <italic>Bordetella bronchiseptica<\/italic> in swine","volume":"80","author":"TL Nicholson","year":"2012","journal-title":"Infect Immun"},{"issue":"3","key":"ref28","doi-asserted-by":"crossref","first-page":"970","DOI":"10.1128\/jb.174.3.970-979.1992","article-title":"Constitutive sensory transduction mutations in the <italic>Bordetella pertussis bvgS<\/italic> gene","volume":"174","author":"JF Miller","year":"1992","journal-title":"J Bacteriol"},{"issue":"1","key":"ref29","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/0378-1119(94)90870-2","article-title":"Mutations in the linker region of BvgS abolish response to environmental signals for the regulation of the virulence factors in <italic>Bordetella pertussis<\/italic>","volume":"150","author":"R Manetti","year":"1994","journal-title":"Gene"},{"issue":"2","key":"ref30","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1128\/MMBR.57.2.320-346.1993","article-title":"Structural, functional, and evolutionary relationships among extracellular solute-binding receptors of bacteria","volume":"57","author":"R Tam","year":"1993","journal-title":"Microbiol Rev"},{"issue":"2","key":"ref31","doi-asserted-by":"crossref","first-page":"E2","DOI":"10.1208\/ps010202","article-title":"The Venus flytrap of periplasmic binding proteins: an ancient protein module present in multiple drug receptors","volume":"1","author":"CB Felder","year":"1999","journal-title":"AAPS PharmSci"},{"issue":"12","key":"ref32","doi-asserted-by":"crossref","first-page":"1987","DOI":"10.1093\/infdis\/jiy125","article-title":"Adaptation of <italic>Bordetella pertussis<\/italic> to the Respiratory Tract","volume":"217","author":"LF van Beek","year":"2018","journal-title":"J Infect Dis"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.jprot.2016.02.002","article-title":"Proteome analysis of <italic>Bordetella pertussis<\/italic> isolated from human macrophages","volume":"136","author":"Y Lamberti","year":"2016","journal-title":"J Proteomics"},{"issue":"4","key":"ref34","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1134\/S102279541603008X","article-title":"Insertional inactivation of virulence operon in population of persistent <italic>Bordetella pertussis<\/italic> bacteria","volume":"52","author":"GI Karataev","year":"2016","journal-title":"Russian Journal of Genetics"},{"issue":"3","key":"ref35","doi-asserted-by":"crossref","first-page":"751","DOI":"10.3390\/vaccines3030751","article-title":"New Data on Vaccine Antigen Deficient <italic>Bordetella pertussis<\/italic> Isolates","volume":"3","author":"V Bouchez","year":"2015","journal-title":"Vaccines (Basel)"},{"issue":"4","key":"ref36","first-page":"626","article-title":"Rapid increase in pertactin-deficient <italic>Bordetella pertussis<\/italic> isolates, Australia","volume":"20","author":"C Lam","year":"2014","journal-title":"Emerg Infect Dis"},{"issue":"2","key":"ref37","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1093\/cid\/ciu788","article-title":"Pertactin-negative <italic>Bordetella pertussis<\/italic> strains: evidence for a possible selective advantage","volume":"60","author":"SW Martin","year":"2015","journal-title":"Clin Infect Dis"},{"issue":"2","key":"ref38","doi-asserted-by":"crossref","first-page":"e31985","DOI":"10.1371\/journal.pone.0031985","article-title":"Prevalence and genetic characterization of pertactin-deficient <italic>Bordetella pertussis<\/italic> in Japan","volume":"7","author":"N Otsuka","year":"2012","journal-title":"PLoS ONE"},{"issue":"2","key":"ref39","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1128\/CVI.00717-13","article-title":"Prevalence and molecular characterization of pertactin-deficient <italic>Bordetella pertussis<\/italic> in the United States","volume":"21","author":"LC Pawloski","year":"2014","journal-title":"Clin Vaccine Immunol"},{"issue":"1","key":"ref40","doi-asserted-by":"crossref","first-page":"e84523","DOI":"10.1371\/journal.pone.0084523","article-title":"Differentially expressed genes in <italic>Bordetella pertussis<\/italic> strains belonging to a lineage which recently spread globally","volume":"9","author":"D de Gouw","year":"2014","journal-title":"PLoS ONE"},{"issue":"1","key":"ref41","doi-asserted-by":"crossref","first-page":"e83449","DOI":"10.1371\/journal.pone.0083449","article-title":"A phase I clinical study of a live attenuated <italic>Bordetella pertussis<\/italic> vaccine BPZE1; a single centre, double-blind, placebo-controlled, dose-escalating study of BPZE1 given intranasally to healthy adult male volunteers","volume":"9","author":"R Thorstensson","year":"2014","journal-title":"PLoS One"},{"issue":"1","key":"ref42","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1093\/infdis\/jix254","article-title":"Live Attenuated Pertussis Vaccine BPZE1 Protects Baboons Against <italic>Bordetella pertussis<\/italic> Disease and Infection","volume":"216","author":"C Locht","year":"2017","journal-title":"J Infect Dis"},{"issue":"4","key":"ref43","doi-asserted-by":"crossref","first-page":"e2000420","DOI":"10.1371\/journal.pbio.2000420","article-title":"<italic>Bordetella bronchiseptica<\/italic> exploits the complex life cycle of <italic>Dictyostelium discoideum<\/italic> as an amplifying transmission vector","volume":"15","author":"DL Taylor-Mulneix","year":"2017","journal-title":"PLoS Biol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"2762","DOI":"10.1128\/IAI.66.6.2762-2768.1998","article-title":"Neither the Bvg<sup>-<\/sup> phase nor the <italic>vrg6<\/italic> locus of <italic>Bordetella pertussis<\/italic> is required for respiratory infection in mice","volume":"66","author":"G Martinez de Tejada","year":"1998","journal-title":"Infect Immun"},{"issue":"19","key":"ref45","doi-asserted-by":"crossref","first-page":"8048","DOI":"10.1074\/jbc.M117.781559","article-title":"The extent of the temperature-induced membrane remodeling in two closely related <italic>Bordetella<\/italic> species reflects their adaptation to diverse environmental niches","volume":"292","author":"G Seydlova","year":"2017","journal-title":"J Biol Chem"},{"issue":"8","key":"ref46","doi-asserted-by":"crossref","first-page":"E1519","DOI":"10.1073\/pnas.1609565114","article-title":"<italic>Bordetella<\/italic> PlrSR regulatory system controls BvgAS activity and virulence in the lower respiratory tract","volume":"114","author":"MA Bone","year":"2017","journal-title":"Proc Natl Acad Sci U S A"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0204861","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,3]],"date-time":"2022-09-03T21:07:31Z","timestamp":1662239251000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0204861"}},"subtitle":[],"editor":[{"given":"Eliane N.","family":"Miyaji","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2018,10,11]]},"references-count":46,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2018,10,11]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0204861","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,11]]}}}