{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T09:57:41Z","timestamp":1790157461943,"version":"4.1.0"},"reference-count":55,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2018,10,5]],"date-time":"2018-10-05T00:00:00Z","timestamp":1538697600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81670858"],"award-info":[{"award-number":["81670858"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81500721"],"award-info":[{"award-number":["81500721"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0205133","type":"journal-article","created":{"date-parts":[[2018,10,5]],"date-time":"2018-10-05T18:10:04Z","timestamp":1538763004000},"page":"e0205133","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":29,"title":["Did you choose appropriate tracer for retrograde tracing of retinal ganglion cells? The differences between cholera toxin subunit B and Fluorogold"],"prefix":"10.1371","volume":"13","author":[{"given":"Fei","family":"Yao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Endong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaolin","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongpei","family":"Ji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahmoud","family":"Marmouri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2239-6451","authenticated-orcid":true,"given":"Xiaobo","family":"Xia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2018,10,5]]},"reference":[{"issue":"5\u20136","key":"ref1","doi-asserted-by":"crossref","first-page":"406","DOI":"10.3109\/08820538.2013.825294","article-title":"Genetic advances in ophthalmology: the role of melanopsin-expressing, intrinsically photosensitive retinal ganglion cells in the circadian organization of the visual system","volume":"28","author":"DJ Ramsey","year":"2013","journal-title":"Semin Ophthalmol"},{"issue":"11","key":"ref2","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1016\/j.tins.2011.07.001","article-title":"Intrinsically photosensitive retinal ganglion cells: many subtypes, diverse functions","volume":"34","author":"TM Schmidt","year":"2011","journal-title":"Trends Neurosci"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1146\/annurev-neuro-071714-034120","article-title":"The types of retinal ganglion cells: current status and implications for neuronal classification","volume":"38","author":"JR Sanes","year":"2015","journal-title":"Annu Rev Neurosci"},{"issue":"4","key":"ref4","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/S1350-9462(03)00027-2","article-title":"Molecular determinants of retinal ganglion cell development, survival, and regeneration","volume":"22","author":"S Isenmann","year":"2003","journal-title":"Prog Retin Eye Res"},{"key":"ref5","article-title":"Rapid learning curve assessment in an ex vivo training system for microincisional glaucoma surgery","volume":"7","author":"YL Dang","year":"2017","journal-title":"Sci Rep-Uk"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/bs.pbr.2015.05.005","article-title":"Retinal ganglion cell apoptotic pathway in glaucoma: Initiating and downstream mechanisms","volume":"220","author":"H Levkovitch-Verbin","year":"2015","journal-title":"Prog Brain Res"},{"issue":"5","key":"ref7","doi-asserted-by":"crossref","first-page":"2117","DOI":"10.1159\/000484231","article-title":"Long Non-Coding RNA-MALAT1 Mediates Retinal Ganglion Cell Apoptosis Through the PI3K\/Akt Signaling Pathway in Rats with Glaucoma","volume":"43","author":"HB Li","year":"2017","journal-title":"Cell Physiol Biochem"},{"issue":"3","key":"ref8","first-page":"194","article-title":"Traumatic optic neuropathy","volume":"88","author":"H Wilhelm","year":"2009","journal-title":"Laryngorhinootologie"},{"issue":"8","key":"ref9","doi-asserted-by":"crossref","first-page":"e69938","DOI":"10.1371\/journal.pone.0069938","article-title":"Intravitreal transplantation of human umbilical cord blood stem cells protects rats from traumatic optic neuropathy","volume":"8","author":"B Jiang","year":"2013","journal-title":"Plos One"},{"issue":"1","key":"ref10","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1097\/WNO.0000000000000069","article-title":"Diffusion tensor imaging in traumatic optic tract syndrome","volume":"34","author":"N Al-Zubidi","year":"2014","journal-title":"J Neuroophthalmol"},{"issue":"1","key":"ref11","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.preteyeres.2008.11.002","article-title":"Ischemic optic neuropathy","volume":"28","author":"SS Hayreh","year":"2009","journal-title":"Prog Retin Eye Res"},{"issue":"9","key":"ref12","doi-asserted-by":"crossref","first-page":"e58","DOI":"10.1097\/TP.0b013e31826dde21","article-title":"Tacrolimus-associated ischemic optic neuropathy and posterior reversible encephalopathy syndrome after small bowel transplantation","volume":"94","author":"X Shao","year":"2012","journal-title":"Transplantation"},{"key":"ref13","article-title":"Intravitreal Triamcinolone Acetonide Injection in a Rodent Model of Anterior Ischemic Optic Neuropathy","author":"LS Pereira","year":"2018","journal-title":"J Neuroophthalmol"},{"issue":"8","key":"ref14","doi-asserted-by":"crossref","first-page":"4592","DOI":"10.1167\/iovs.15-16808","article-title":"Inherited Photoreceptor Degeneration Causes the Death of Melanopsin-Positive Retinal Ganglion Cells and Increases Their Coexpression of Brn3a","volume":"56","author":"D Garcia-Ayuso","year":"2015","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.exer.2017.05.010","article-title":"Light-induced retinal degeneration causes a transient downregulation of melanopsin in the rat retina","volume":"161","author":"D Garcia-Ayuso","year":"2017","journal-title":"Exp Eye Res"},{"issue":"2","key":"ref16","first-page":"799","article-title":"Adeno-associated virus mediated SOD gene therapy protects the retinal ganglion cells from chronic intraocular pressure elevation induced injury via attenuating oxidative stress and improving mitochondrial dysfunction in a rat model","volume":"8","author":"W Jiang","year":"2016","journal-title":"Am J Transl Res"},{"issue":"2","key":"ref17","first-page":"239","article-title":"Neuroprotective effect of edaravone in experimental glaucoma model in rats: a immunofluorescence and biochemical analysis","volume":"8","author":"AT Aksar","year":"2015","journal-title":"Int J Ophthalmol"},{"issue":"5","key":"ref18","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1111\/ceo.12915","article-title":"Glymphatic stasis at the site of the lamina cribrosa as a potential mechanism underlying open-angle glaucoma","volume":"45","author":"P Wostyn","year":"2017","journal-title":"Clin Exp Ophthalmol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.exer.2016.08.006","article-title":"Evaluating retinal ganglion cell loss and dysfunction","volume":"151","author":"B Mead","year":"2016","journal-title":"Exp Eye Res"},{"issue":"1","key":"ref20","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.jphysparis.2013.10.001","article-title":"Mapping the primate lateral geniculate nucleus: a review of experiments and methods","volume":"108","author":"AM Jeffries","year":"2014","journal-title":"J Physiol Paris"},{"issue":"4","key":"ref21","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1016\/0306-4522(84)90007-1","article-title":"Retinal ganglion cells that project to the superior colliculus and pretectum in the macaque monkey","volume":"12","author":"VH Perry","year":"1984","journal-title":"Neuroscience"},{"issue":"1","key":"ref22","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.visres.2008.09.029","article-title":"A computerized analysis of the entire retinal ganglion cell population and its spatial distribution in adult rats","volume":"49","author":"M Salinas-Navarro","year":"2009","journal-title":"Vision Res"},{"issue":"49","key":"ref23","doi-asserted-by":"crossref","first-page":"16573","DOI":"10.1523\/JNEUROSCI.3305-10.2010","article-title":"Visual receptive field properties of neurons in the superficial superior colliculus of the mouse","volume":"30","author":"L Wang","year":"2010","journal-title":"J Neurosci"},{"issue":"2\u20133","key":"ref24","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/S0165-0173(00)00028-X","article-title":"Molecular mechanisms regulating the retrograde axonal transport of neurotrophins","volume":"33","author":"AJ Reynolds","year":"2000","journal-title":"Brain Res Brain Res Rev"},{"issue":"2","key":"ref25","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1364\/BOE.4.000364","article-title":"Imaging axonal transport in the rat visual pathway","volume":"4","author":"CJ Abbott","year":"2013","journal-title":"Biomed Opt Express"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/bs.pbr.2015.04.008","article-title":"Retinal neurodegeneration in experimental glaucoma","volume":"220","author":"M Vidal-Sanz","year":"2015","journal-title":"Prog Brain Res"},{"issue":"4","key":"ref27","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/S0301-0082(00)00019-8","article-title":"Current concepts in neuroanatomical tracing","volume":"62","author":"C Kobbert","year":"2000","journal-title":"Prog Neurobiol"},{"issue":"1","key":"ref28","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.aanat.2012.04.005","article-title":"Mediodorsal thalamic nucleus receives a direct retinal input in marmoset monkey (Callithrix jacchus): a subunit B cholera toxin study","volume":"195","author":"TB de Sousa","year":"2013","journal-title":"Ann Anat"},{"issue":"11","key":"ref29","doi-asserted-by":"crossref","first-page":"2514","DOI":"10.1002\/cne.24195","article-title":"The centrifugal visual system of a palaeognathous bird, the Chilean Tinamou (Nothoprocta perdicaria)","volume":"525","author":"Q Krabichler","year":"2017","journal-title":"Journal of Comparative Neurology"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.brainres.2015.12.015","article-title":"Postconditioning with inhaled hydrogen promotes survival of retinal ganglion cells in a rat model of retinal ischemia\/reperfusion injury","volume":"1632","author":"RB Wang","year":"2016","journal-title":"Brain Research"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.exer.2015.10.004","article-title":"Neuroprotective effect of 4-(Phenylsulfanyl)butan-2-one on optic nerve crush model in rats","volume":"143","author":"JY Chien","year":"2016","journal-title":"Exp Eye Res"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.exer.2014.12.005","article-title":"Two methods to trace retinal ganglion cells with fluorogold: from the intact optic nerve or by stereotactic injection into the optic tract","volume":"131","author":"FM Nadal-Nicolas","year":"2015","journal-title":"Exp Eye Res"},{"issue":"7","key":"ref33","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1007\/s00417-011-1635-7","article-title":"Protective effects of human umbilical cord blood stem cell intravitreal transplantation against optic nerve injury in rats","volume":"249","author":"T Zhao","year":"2011","journal-title":"Graefes Arch Clin Exp Ophthalmol"},{"issue":"3","key":"ref34","doi-asserted-by":"crossref","first-page":"310","DOI":"10.3390\/toxins2030310","article-title":"Cholera toxin: an intracellular journey into the cytosol by way of the endoplasmic reticulum","volume":"2","author":"NL Wernick","year":"2010","journal-title":"Toxins (Basel)"},{"issue":"3","key":"ref35","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.jchemneu.2011.07.001","article-title":"A half century of experimental neuroanatomical tracing","volume":"42","author":"JL Lanciego","year":"2011","journal-title":"Journal of Chemical Neuroanatomy"},{"issue":"12","key":"ref36","doi-asserted-by":"crossref","first-page":"1756","DOI":"10.1002\/cne.23723","article-title":"Retinal projections in the short-tailed fruit bat, Carollia perspicillata, as studied using the axonal transport of cholera toxin B subunit: Comparison with mouse","volume":"523","author":"F Scalia","year":"2015","journal-title":"J Comp Neurol"},{"issue":"1","key":"ref37","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/0006-8993(86)91199-6","article-title":"Fluoro-Gold: a new fluorescent retrograde axonal tracer with numerous unique properties","volume":"377","author":"LC Schmued","year":"1986","journal-title":"Brain Res"},{"issue":"1","key":"ref38","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/0006-8993(91)90241-M","article-title":"Fluoro-Gold: composition, and mechanism of uptake","volume":"553","author":"MW Wessendorf","year":"1991","journal-title":"Brain Res"},{"key":"ref39","article-title":"Comparison of the neuroprotective effects of brimonidine tartrate and melatonin on retinal ganglion cells","author":"D Marangoz","year":"2017","journal-title":"Int Ophthalmol"},{"issue":"2","key":"ref40","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1167\/iovs.13-12811","article-title":"Evaluation of Retinal Nerve Fiber Layer Thickness and Axonal Transport 1 and 2 Weeks After 8 Hours of Acute Intraocular Pressure Elevation in Rats","volume":"55","author":"CJ Abbott","year":"2014","journal-title":"Invest Ophth Vis Sci"},{"issue":"11","key":"ref41","article-title":"Comparison of Longitudinal In Vivo Measurements of Retinal Nerve Fiber Layer Thickness and Retinal Ganglion Cell Density after Optic Nerve Transection in Rat","volume":"9","author":"TE Choe","year":"2014","journal-title":"Plos One"},{"issue":"2","key":"ref42","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.brainres.2005.09.046","article-title":"Tracer coupling of neurons in the rat retina inner nuclear layer labeled by Fluorogold","volume":"1063","author":"RM Abdel-Majid","year":"2005","journal-title":"Brain Res"},{"issue":"14","key":"ref43","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.4103\/1673-5374.121613","article-title":"Neurological function following intra-neural injection of fluorescent neuronal tracers in rats","volume":"8","author":"W Hu","year":"2013","journal-title":"Neural Regen Res"},{"issue":"1","key":"ref44","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1186\/s12974-017-0982-7","article-title":"Microglial dynamics after axotomy-induced retinal ganglion cell death","volume":"14","author":"FM Nadal-Nicolas","year":"2017","journal-title":"J Neuroinflammation"},{"issue":"2","key":"ref45","first-page":"414","article-title":"Effects of intramuscular injection of botulinum toxin and doxorubicin on the survival of abducens motoneurons","volume":"40","author":"AM Gomez-Ramirez","year":"1999","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.exer.2013.11.012","article-title":"Neuroprotective effects of recombinant human granulocyte colony-stimulating factor (G-CSF) in a rat model of anterior ischemic optic neuropathy (rAION)","volume":"118","author":"CH Chang","year":"2014","journal-title":"Exp Eye Res"},{"issue":"2","key":"ref47","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/j.expneurol.2004.02.002","article-title":"GDNF, Ret, GFRalpha1 and 2 in the adult rat retino-tectal system after optic nerve transection","volume":"187","author":"N Lindqvist","year":"2004","journal-title":"Exp Neurol"},{"issue":"9","key":"ref48","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1007\/s00417-007-0746-7","article-title":"The neuropeptide NAP provides neuroprotection against retinal ganglion cell damage after retinal ischemia and optic nerve crush","volume":"246","author":"T Jehle","year":"2008","journal-title":"Graefes Arch Clin Exp Ophthalmol"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.exer.2017.03.008","article-title":"Methane rescues retinal ganglion cells and limits retinal mitochondrial dysfunction following optic nerve crush","volume":"159","author":"R Wang","year":"2017","journal-title":"Exp Eye Res"},{"issue":"5","key":"ref50","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1177\/1535370214560965","article-title":"Atoh7 promotes the differentiation of Muller cells-derived retinal stem cells into retinal ganglion cells in a rat model of glaucoma","volume":"240","author":"WT Song","year":"2015","journal-title":"Exp Biol Med (Maywood)"},{"issue":"3","key":"ref51","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1002\/cne.20970","article-title":"Central projections of melanopsin-expressing retinal ganglion cells in the mouse","volume":"497","author":"S Hattar","year":"2006","journal-title":"Journal of Comparative Neurology"},{"issue":"13","key":"ref52","doi-asserted-by":"crossref","first-page":"8373","DOI":"10.1167\/iovs.15-16614","article-title":"The Dorsal Raphe Nucleus Receives Afferents From Alpha-Like Retinal Ganglion Cells and Intrinsically Photosensitive Retinal Ganglion Cells in the Rat","volume":"56","author":"XT Li","year":"2015","journal-title":"Invest Ophth Vis Sci"},{"issue":"7","key":"ref53","article-title":"Dendritic Morphology of Caudal Periaqueductal Gray Projecting Retinal Ganglion Cells in Mongolian Gerbil (Meriones unguiculatus)","volume":"9","author":"CR Ren","year":"2014","journal-title":"Plos One"},{"issue":"8","key":"ref54","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1038\/nprot.2009.93","article-title":"Multiple neuroanatomical tract-tracing using fluorescent Alexa Fluor conjugates of cholera toxin subunit B in rats","volume":"4","author":"WL Conte","year":"2009","journal-title":"Nat Protoc"},{"issue":"6","key":"ref55","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1016\/j.neuron.2015.02.017","article-title":"Subtype-specific regeneration of retinal ganglion cells following axotomy: effects of osteopontin and mTOR signaling","volume":"85","author":"X Duan","year":"2015","journal-title":"Neuron"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0205133","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,3]],"date-time":"2022-09-03T11:47:07Z","timestamp":1662205627000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0205133"}},"subtitle":[],"editor":[{"given":"Alfred S","family":"Lewin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2018,10,5]]},"references-count":55,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2018,10,5]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0205133","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,5]]}}}