{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:35:23Z","timestamp":1790710523926,"version":"4.1.0"},"reference-count":44,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2018,10,4]],"date-time":"2018-10-04T00:00:00Z","timestamp":1538611200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0205136","type":"journal-article","created":{"date-parts":[[2018,10,4]],"date-time":"2018-10-04T18:55:31Z","timestamp":1538679331000},"page":"e0205136","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":32,"title":["Rapid identification of Bactrocera zonata (Dip.: Tephritidae) using TaqMan real-time PCR assay"],"prefix":"10.1371","volume":"13","author":[{"given":"Marzieh","family":"Koohkanzade","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Zakiaghl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manpreet K.","family":"Dhami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5189-9488","authenticated-orcid":true,"given":"Lida","family":"Fekrat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hussein Sadeghi","family":"Namaghi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2018,10,4]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1111\/j.1439-0418.2008.01325.x","article-title":"Life-history evolution of <italic>C<\/italic>. <italic>capitata<\/italic>","volume":"132","author":"AD Diamantidis","year":"2008","journal-title":"J Appl Entomol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1016\/S0261-2194(02)00019-4","article-title":"\u201cSingle-killing-point\u201d field assessment of bait and lure control of fruit flies (Diptera:Tephritidae) in Pakistan","volume":"21","author":"J Stonehouse","year":"2002","journal-title":"Crop Prot"},{"issue":"2","key":"ref3","doi-asserted-by":"crossref","first-page":"297","DOI":"10.3390\/insects6020297","article-title":"An overview of pest species of <italic>Bactrocera<\/italic> fruit flies (Diptera: Tephritidae) and the integration of biopesticides with other biological approaches for their management with a focus on the Pacific region","volume":"6","author":"RI Vargas","year":"2015","journal-title":"Insects"},{"key":"ref4","article-title":"Areawide Pest Management: Theory and Implementation","author":"RI Vargas","year":"2008"},{"key":"ref5","doi-asserted-by":"crossref","DOI":"10.1079\/9780851987903.0000","article-title":"Fruit flies of economic significance: their Identification and bionomics","author":"IM White","year":"1992"},{"issue":"2","key":"ref6","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1603\/AN11100","article-title":"Molecular identification of <italic>Ceratitis capitata<\/italic> (Diptera: Tephritidae) using DNA sequences of the COI barcode region","volume":"105","author":"NB Barr","year":"2012","journal-title":"Ann Entomol Soc Am"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1111\/j.1755-0998.2012.03124.x","article-title":"Barcoding Queensland fruit flies (<italic>Bactrocera tryoni<\/italic>): impediments and improvements","volume":"12","author":"M Blacket","year":"2012","journal-title":"Mol Ecol Resour"},{"issue":"1","key":"ref8","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1111\/j.1365-2664.2008.01600.x","article-title":"Trade, transport and trouble: managing invasive species pathways in an era of globalization","volume":"46","author":"PE Hulme","year":"2009","journal-title":"J Appl Ecol"},{"issue":"2","key":"ref9","doi-asserted-by":"crossref","first-page":"342","DOI":"10.4001\/003.023.0225","article-title":"Molecular identification of tephritid fruit flies (Diptera: Tephritidae) infesting sweet oranges in Nsukka Agro-Ecological Zone, Nigeria, based on PCR-RFLP of COI gene and DNA barcoding","volume":"23","author":"IE Onah","year":"2015","journal-title":"African Entomol"},{"key":"ref10","article-title":"Proc NZ Plant Prot Conf. Lincoln","author":"K Armstrong","year":"1997"},{"issue":"6","key":"ref11","doi-asserted-by":"crossref","first-page":"e98934","DOI":"10.1371\/journal.pone.0098934","article-title":"A HRM Real-Time PCR Assay for Rapid and specific identification of the emerging pest spotted-wing Drosophila (<italic>Drosophila suzukii<\/italic>)","volume":"9","author":"MK Dhami","year":"2014","journal-title":"PLoS ONE"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1813","DOI":"10.1098\/rstb.2005.1713","article-title":"DNA barcodes for biosecurity: Invasive species identification","volume":"360","author":"KF Armstrong","year":"2005","journal-title":"Philos Trans R Soc Lond B: Biol Sci"},{"issue":"4","key":"ref13","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1002\/ps.1886","article-title":"Species determination of Malaysian <italic>Bactrocera<\/italic> pests using PCR-RFLP analyses (Diptera: Tephritidae)","volume":"66","author":"TH Chua","year":"2009","journal-title":"Pest Manag Sci"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1111\/epp.12030","article-title":"Q-bank, a database with information for identification of plant quarantine plant pest and diseases","volume":"43","author":"P Bonants","year":"2013","journal-title":"EPPO Bull"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1111\/mpp.12057","article-title":"Top 10 plant-parasitic nematodes in molecular plant pathology","volume":"14","author":"JT Jones","year":"2013","journal-title":"Mol Plant Pathol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1603\/EC12119","article-title":"Application of cytochrome oxidase I sequences for phylogenetic analysis and identification of thrips species occurring on vegetable crops","volume":"106","author":"P Kadirvel","year":"2013","journal-title":"J Econ Entomol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"279","DOI":"10.3897\/zookeys.365.5819","article-title":"Half of the European fruit fly species barcoded (Diptera, Tephritidae); a feasibility test for molecular identification","volume":"365","author":"J Smit","year":"2013","journal-title":"Zookeys"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1111\/j.1471-8286.2004.00732.x","article-title":"Polymorphic microsatellite DNA markers from the oriental fruit fly <italic>Bactrocera dorsalis<\/italic> (Hendel)","volume":"4","author":"SM Dai","year":"2004","journal-title":"Mol Ecol Notes"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1007\/s12686-013-0026-2","article-title":"Isolation and characterisation of sixteen microsatellite markers amplifying an African agricultural pest, <italic>Ceratitis cosyra<\/italic> (Walker) (Diptera: Tephritidae)","volume":"6","author":"H Delatte","year":"2014","journal-title":"Conservation Genet Resour"},{"key":"ref20","first-page":"4","article-title":"Microsatellite markers for the pest fruit fly, <italic>Bactrocera papayae<\/italic> (Diptera: Tephritidae) and other <italic>Bactrocera<\/italic> species","volume":"6","author":"DC Shearman","year":"2006","journal-title":"Mol Ecol Res"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1098\/rsbl.2009.1060","article-title":"Taqman real-time quantitative PCR for identification of western flower thrip (<italic>Frankliniella occidentalis<\/italic>) for plant quarantine","volume":"6","author":"KS Huang","year":"2010","journal-title":"Biol Lett"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1111\/j.1439-0418.2005.00960.x","article-title":"Development of a sequence-specific real-time PCR to the melon thrips, <italic>Thrips palmi<\/italic> (Thysan., Thripidae)","volume":"129","author":"K Walsh","year":"2005","journal-title":"J Appl Entomol"},{"issue":"5","key":"ref23","doi-asserted-by":"crossref","first-page":"1706","DOI":"10.1603\/EC11093","article-title":"A multiplex real-time polymerase chain reaction assay to diagnose <italic>Epiphyas postvittana<\/italic> (Lepidoptera: Tortricidae)","volume":"104","author":"NB Barr","year":"2011","journal-title":"J Econ Entomol"},{"issue":"4","key":"ref24","doi-asserted-by":"crossref","first-page":"1957","DOI":"10.1093\/jee\/tow111","article-title":"Diagnosis of <italic>Lobesia botrana<\/italic> (Lepidoptera: Tortricidae) using Real-Time PCR","volume":"109","author":"LA Ledezma","year":"2016","journal-title":"J Econ Entomol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1111\/j.1439-0418.2012.01706.x","article-title":"Differentiation between <italic>Aphis pomi<\/italic> and <italic>Aphis spiraecola<\/italic> using multiplex real-time PCR based on DNA barcode sequences","volume":"136","author":"AM Naaum","year":"2012","journal-title":"J Appl Entomol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1111\/jen.12114","article-title":"Real-time PCR for identification of the soybean aphid, <italic>Aphis glycines<\/italic> Matsumura","volume":"138","author":"AM Naaum","year":"2014","journal-title":"J Appl Entomol"},{"issue":"7","key":"ref27","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1111\/jen.12286","article-title":"A real-time PCR toolbox for accurate identification of invasive fruit fly species","volume":"140","author":"MK Dhami","year":"2016","journal-title":"J Appl Entomol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1111\/j.1439-0418.2004.00907.x","article-title":"Rapid identification of <italic>Bactrocera latifrons<\/italic> (Dipt., Tephritidae) by real\u2010time PCR using SYBR Green chemistry","volume":"128","author":"D Yu","year":"2004","journal-title":"J Appl Entomol"},{"key":"ref29","first-page":"73","article-title":"Real-time qualitative PCR for the inspection and identification of <italic>Bactrocera phillippinensis<\/italic> and <italic>Bactrocera occipitalis<\/italic> (Diptera: Tephritidae) using SYBR green assay","volume":"53","author":"D Yu","year":"2005","journal-title":"Raffles Bull Zool"},{"issue":"11","key":"ref30","doi-asserted-by":"crossref","first-page":"e0142912","DOI":"10.1371\/journal.pone.0142912","article-title":"A multiplex Real-Time PCR assay to diagnose and separate <italic>Helicoverpa armigera<\/italic> and <italic>H<\/italic>. <italic>zea<\/italic> (Lepidoptera: Noctuidae) in the New World","volume":"10","author":"TM Gilligan","year":"2015","journal-title":"PLoS ONE"},{"key":"ref31","article-title":"Real-time PCR an essential guide","author":"JMJ Logan","year":"2004"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"339","DOI":"10.3897\/zookeys.540.9786","article-title":"A phylogenetic assessment of the polyphyletic nature and intraspecific color polymorphism in the <italic>Bactrocera dorsalis<\/italic> complex (Diptera, Tephritidae)","volume":"540","author":"L Leblanc","year":"2015","journal-title":"ZooKeys"},{"issue":"5","key":"ref33","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1080\/24701394.2017.1350952","article-title":"Genetic analysis of <italic>Bactrocera zonata<\/italic> (Diptera: Tephritidae) populations from India based on cox1 and nad1 gene sequences","volume":"29","author":"JS Choudhary","year":"2018","journal-title":"Mitochondr DNA"},{"issue":"3","key":"ref34","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1653\/024.093.0308","article-title":"Molecular Phylogeny of <italic>Bactrocera<\/italic> Species (Diptera: Tephritidae: Dacini) Inferred from Mitochondrial Sequences of 16S rDNA and COI Sequences","volume":"93","author":"B Zhang","year":"2010","journal-title":"Fla Entomol"},{"issue":"1","key":"ref35","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1093\/jee\/tou027","article-title":"Mitochondrial single nucleotide polymorphisms in <italic>Ceratitis capitata<\/italic> (Diptera: Tephritidae) can distinguish sterile, released flies from wild flies in various regions of the world","volume":"108","author":"A Parubrub","year":"2015","journal-title":"J Econ Entomol"},{"issue":"4","key":"ref36","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1002\/ps.1886","article-title":"Species determination of Malaysian <italic>Bactrocera<\/italic> pests using PCR-RFLP analyses (Diptera: Tephritidae)","volume":"66","author":"TH Chua","year":"2010","journal-title":"Pest Manag Sci"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1111\/j.1365-2583.1996.tb00049.x","article-title":"The insect cytochrome oxidase I gene: Evolutionary patterns and conserved primers for phylogenetic studies","volume":"5","author":"DH Lunt","year":"1996","journal-title":"Insect Mol Biol"},{"issue":"4","key":"ref38","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.1653\/024.094.0441","article-title":"Molecular identification and phylogeny of <italic>Bactrocera<\/italic> species (Diptera: Tephritidae)","volume":"94","author":"R Asokan","year":"2011","journal-title":"Fla Entomol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"1549","DOI":"10.1093\/bioinformatics\/btn227","article-title":"QPCR: an R package for sigmoidal model selection in quantitative real-time polymerase chain reaction analysis","volume":"24","author":"C Ritz","year":"2008","journal-title":"Bioinformatics"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1373\/clinchem.2008.112797","article-title":"The MIQE guidelines: minimum in formation for publication of quantitative real-time PCR experiments","volume":"55","author":"SA Bustin","year":"2009","journal-title":"Clin Chem"},{"key":"ref41","article-title":"Real-Time PCR Assay for the Identification of the Brown Marmorated Stink Bug (<italic>Halyomorpha halys<\/italic>)","author":"MK Dhami","year":"2016","journal-title":"Front Mol Biosci"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"37549","DOI":"10.1038\/srep37549","article-title":"Selection of reliable reference genes for normalization of quantitative RT\u2010PCR from different developmental stages and tissues in amphioxus","volume":"6","author":"QL Zhang","year":"2016","journal-title":"Sci Rep"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1017\/S0007485318000251","article-title":"DNA barcoding and real-time PCR detection of <italic>Bactrocera xanthodes<\/italic> (Tephritidae: Diptera) complex","author":"D Li","year":"2018","journal-title":"Bull Entomol Res"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1186\/1472-6750-7-76","article-title":"Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications","volume":"7","author":"F Mao","year":"2007","journal-title":"BMC Biotech"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0205136","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,3]],"date-time":"2022-09-03T09:04:25Z","timestamp":1662195865000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0205136"}},"subtitle":[],"editor":[{"given":"Bi-Song","family":"Yue","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2018,10,4]]},"references-count":44,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2018,10,4]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0205136","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,4]]}}}