{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T14:13:07Z","timestamp":1791987187915,"version":"4.4.0"},"reference-count":57,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2018,10,10]],"date-time":"2018-10-10T00:00:00Z","timestamp":1539129600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0205372","type":"journal-article","created":{"date-parts":[[2018,10,10]],"date-time":"2018-10-10T14:33:27Z","timestamp":1539182007000},"page":"e0205372","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":18,"title":["Postnatal changes in the development of rat submandibular glands in offspring of diabetic mothers: Biochemical, histological and ultrastructural study"],"prefix":"10.1371","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2098-2155","authenticated-orcid":true,"given":"Abir","family":"El Sadik","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enas","family":"Mohamed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"El Zainy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2018,10,10]]},"reference":[{"issue":"2","key":"ref1","doi-asserted-by":"crossref","first-page":"604","DOI":"10.4067\/S0717-95022011000200051","article-title":"Histochemical localization of carbohydrates in morphological stages of developing human minor salivary glands: a comparative study with cytoskeletal markers","volume":"29","author":"MJBM R\u00eago","year":"2011","journal-title":"Int J Morphol"},{"key":"ref2","article-title":"Oral development and histology","author":"RM Klein","year":"2002"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1146\/annurev-food-030212-182700","article-title":"The secretion, components, and properties of saliva","volume":"4","author":"GH Carpenter","year":"2013","journal-title":"Annu Rev Food Sci Technol"},{"issue":"1","key":"ref4","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1046\/j.1365-2842.1998.00203.x","article-title":"The masticatory-parotid salivary reflex in edentulous subjects","volume":"25","author":"BJJ Scott","year":"1998","journal-title":"J Oral Rehabil"},{"issue":"4","key":"ref5","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1007\/s00795-003-0225-0","article-title":"Defensins in saliva and the salivary glands","volume":"36","author":"Y Abiko","year":"2003","journal-title":"Med Electron Microsc"},{"issue":"3","key":"ref6","first-page":"92","article-title":"Saliva: an overview","volume":"110","author":"A M L Benn","year":"2014","journal-title":"NZ Dent J"},{"issue":"3","key":"ref7","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1007\/BF02555111","article-title":"Saturation of human salivary secretions with respect to calcite and inhibition of calcium-carbonate precipitation by salivary constituents","volume":"39","author":"DI Hay","year":"1986","journal-title":"Calcif Tissue Int"},{"key":"ref8","first-page":"69","article-title":"Xerostomia\u2014diagnosis and treatment","volume":"38","author":"M Mravak-Stipetic","year":"2012","journal-title":"Medical Sciences"},{"key":"ref9","first-page":"252","article-title":"Histological, histochemical and uitrastructural changes in the submandibular gland of starved young male cats","volume":"5","author":"EA EL-Kordy","year":"2014","journal-title":"J Cytol Histol"},{"issue":"2012","key":"ref10","article-title":"High endogenous salivary amylase activity is associated with improved glycemic homeostasis following starch ingestion in adults","volume":"4","author":"AL Mandel","year":"2012","journal-title":"The Journal of Nutrition"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1002\/star.201000150","article-title":"Human \u03b1-amylase and starch digestion: An interesting marriage","volume":"63","author":"PJ Butterworth","year":"2011","journal-title":"Starch STARKE"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1093\/bmb\/60.1.173","article-title":"Long-term consequences for offspring of diabetes during pregnancy","volume":"60","author":"F Van Assche","year":"2001","journal-title":"British Medical Bulletin"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1849","DOI":"10.2337\/db11-0400","article-title":"Maternal environment and the transgenerational cycle of obesity and diabetes","volume":"60","author":"D Dabelea","year":"2011","journal-title":"Diabetes"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1016\/S0891-5849(00)00397-X","article-title":"Oxidative damage in pregnant diabetic rats and their embryos","volume":"29","author":"M Viana","year":"2000","journal-title":"Free Radic Biol Med"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"930","DOI":"10.1002\/ar.a.20236","article-title":"Stereology and ultrastructure of the salivary glands of diabetic Nod mice submitted to long-term insulin treatment","volume":"286","author":"EJ Caldeira","year":"2005","journal-title":"Anat Rec A Discov Mol Cell Evol Biol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1111\/jop.12238","article-title":"Diabetes causes morphological changes in human submandibular gland: a morphometric study","volume":"44","author":"MA Lilliu","year":"2015","journal-title":"J Oral Pathol Med"},{"issue":"3","key":"ref17","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1016\/j.bbadis.2005.12.002","article-title":"Changes in functioning of rat submandibular salivary gland under streptozotocin-induced diabetes are associated with alterations of Ca2+ signaling and Ca2+ transporting pumps","volume":"1762","author":"NV Fedirko","year":"2006","journal-title":"Biochimica et Biophysica Acta"},{"issue":"3","key":"ref18","doi-asserted-by":"crossref","first-page":"199","DOI":"10.5152\/eurasianjmed.2015.80","article-title":"Effects of Diabetes on Post-Menopausal Rat Submandibular Glands: A Histopathological and Stereological Examination","volume":"47","author":"B Buyuk","year":"2015","journal-title":"Eurasian J Med"},{"issue":"2","key":"ref19","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1007\/BF02913315","article-title":"Induction of diabetes by streptozotocin in rats","volume":"22","author":"A Akbarzadeh","year":"2007","journal-title":"Indian Journal of Clinical Biochemistry"},{"issue":"1","key":"ref20","first-page":"29","article-title":"Antidiabetic activity of trigonella foenumgraecum L. seeds extract (ind01) in neonatal streptozotocin-induced (n-stz) rats","volume":"41","author":"CP Kulkarni","year":"2012","journal-title":"Diabetologia Croatica"},{"issue":"3","key":"ref21","first-page":"398","article-title":"Induction of diabetes mellitus in rats using intraperitoneal streptozotocin: a comparison between 2 strains of rats","volume":"32","author":"M Abu Abeeleh","year":"2009","journal-title":"European Journal of Scientific Research"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1677\/joe.1.06596","article-title":"Sexual dimorphism in insulin sensitivity and susceptibility to develop diabetes in rats","volume":"190","author":"P Vital","year":"2006","journal-title":"Journal of Endocrinology"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1006\/abio.1999.4085","article-title":"Development and validation of real-time quantitative reverse transcriptase-polymerase chain reaction for monitoring gene expression in cardiac myocytes in vitro","volume":"270","author":"J Winer","year":"1999","journal-title":"Anal Biochem"},{"key":"ref24","article-title":"Histological and histochemical methods: theory and practice","author":"JA Kiernan","year":"2015"},{"key":"ref25","article-title":"Theory and practice of histological techniques","author":"JD Bancroft","year":"2013"},{"key":"ref26","article-title":"Manual of histological techniques and diagnostic applications","author":"JD Bancroft","year":"1994"},{"key":"ref27","article-title":"Principles and techniques of electron microscopy: Biological applications","author":"MA Hayat","year":"2000"},{"key":"ref28","article-title":"Statistical methods in medical research","author":"P Armitage","year":"1994"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1097\/01.EHX.0000398761.63208.11","article-title":"Effect of sildenafil (Viagra) on epidermal growth factor expression in submandibular gland of diabetic male rats: histological and immunohistochemical study","volume":"34","author":"DA El-Gamal","year":"2011","journal-title":"The Egyptian Journal of Histology"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.1126\/science.1192046","article-title":"Parasympathetic innervation maintains epithelial progenitor cells during salivary organogenesis","volume":"329","author":"SM Knox","year":"2010","journal-title":"Science"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1242\/bio.20134309","article-title":"Quantitative single cell analysis of cell population dynamics during submandibular salivary gland development and differentiation","volume":"2","author":"DA Nelson","year":"2013","journal-title":"Biology Open"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1111\/j.1365-2613.2008.00613.x","article-title":"Cellular expression of Bcl-2 and Bax in atrophic submandibular glands of rats","volume":"89","author":"S Takahashi","year":"2008","journal-title":"Int. J. Exp. Path"},{"issue":"10","key":"ref33","first-page":"2039","article-title":"Functional regeneration of irradiated salivary glands with human amniotic epithelial cells transplantation","volume":"6","author":"NN Zhang","year":"2013","journal-title":"Int J Clin Exp Pathol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1007\/BF00458557","article-title":"The size and composition of the submandibular glands in late-onset diabetes","volume":"244","author":"H Lindeberg","year":"1987","journal-title":"Arch Otorhinolaryngol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1067\/moe.2001.112054","article-title":"A longitudinal analysis of salivary flow in control subjects and older adults with type 2 diabetes","volume":"91","author":"EM Chavez","year":"2001","journal-title":"Oral Surg Oral Med Oral Pathol Oral Radiol Endod"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1007\/s00232-009-9159-3","article-title":"Na+-glucose cotransporter SGLT1 protein in salivary glands: potential involvement in the diabetes-induced decrease in salivary flow","volume":"228","author":"R Sabino-Silva","year":"2009","journal-title":"J Membr Biol"},{"issue":"2012","key":"ref37","first-page":"079","article-title":"Salivary flow and composition in diabetic and non-diabetic subjects","volume":"27","author":"TJ Lasisi","year":"2012","journal-title":"Niger J Physiol Sci"},{"issue":"6","key":"ref38","first-page":"ZC56","article-title":"Estimation of salivary and serum biomarkers in diabetic and non-diabetic patients\u2014a comparative study","volume":"10","author":"A Ladgotra","year":"2016","journal-title":"Journal of Clinical and Diagnostic Research"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1016\/j.bbagen.2011.01.013","article-title":"Abnormal subcellular localization of AQP5 and downregulated AQP5 protein in parotid glands of streptozotocin-induced diabetic rats","volume":"1810","author":"D Wang","year":"2011","journal-title":"Biochim Biophys Acta"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1007\/s12192-011-0281-7","article-title":"Detection of Hsp60 in saliva and serum from type 2 diabetic and non-diabetic control subjects","volume":"16","author":"J Yuan","year":"2011","journal-title":"Cell Stress Chaperones"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"2012","DOI":"10.1155\/2012\/696215","article-title":"Cellular dysfunction in diabetes as maladaptive response to mitochondrial oxidative stress","volume":"2012","author":"A Naudi","year":"2012","journal-title":"Exp Diabetes Res"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"4583742","DOI":"10.1155\/2016\/4583742","article-title":"Oxidative Damage to the Salivary Glands of Rats with Streptozotocin-Induced Diabetes-Temporal Study: Oxidative Stress and Diabetic Salivary Glands","volume":"2016","author":"M Knas","year":"2016","journal-title":"Journal of Diabetes Research"},{"issue":"4","key":"ref43","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1076\/ejom.38.4.0233","article-title":"Organic secretion of striated ducts","volume":"38","author":"B Tandler","year":"2000","journal-title":"European Journal of Morphology"},{"issue":"2","key":"ref44","first-page":"210","article-title":"Submandibular Glands as an Evident of the Effects of Antioxidant on Alloxan-Induced Diabetic Rats","volume":"11","author":"GAM EL-Gusbi","year":"2014","journal-title":"World Journal of Medical Sciences"},{"issue":"2","key":"ref45","doi-asserted-by":"crossref","first-page":"111","DOI":"10.2334\/josnusd.47.111","article-title":"Altered glycogen metabolism in the submandibular and parotid salivary glands of rats with streptozotocin-induced diabetes","volume":"47","author":"J Nicolau","year":"2005","journal-title":"Journal of oral science"},{"issue":"4","key":"ref46","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1046\/j.1365-2842.2001.00674.x","article-title":"Sato. Accelerated DNA fragmentation of the mucosal epithelium in an animal model of diabetes","volume":"28","author":"YT Maruo","year":"2001","journal-title":"Journal of Oral Rehabit"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/j.ajpath.2011.03.037","article-title":"Expression and regulation of the DN and TAp63 isoforms in salivary gland tumorigenesis clinical and experimental findings","volume":"179","author":"Y Mitani","year":"2011","journal-title":"Am J Pathol"},{"issue":"1","key":"ref48","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/01926230390173798","article-title":"Multifocal Ductal Cell Hyperplasia in the Submandibular Salivary Glands of Wistar Rats Chronically Treated with a Novel Steroidal Compound","volume":"31","author":"EPCT De Rijk","year":"2003","journal-title":"Toxicologic Pathology"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1038\/modpathol.3880483","article-title":"Expression of cytokeratin 5\/6 in epithelial neoplasms: an immunohistochemical study of 509 cases","volume":"15","author":"PG Chu","year":"2002","journal-title":"Mod. Pathol"},{"issue":"2","key":"ref50","doi-asserted-by":"crossref","first-page":"724","DOI":"10.2353\/ajpath.2010.090282","article-title":"Potential Down-Regulation of Salivary Gland AQP5 by LPS via Cross-Coupling of NF-_B and p-c-Jun\/c-Fos","volume":"177","author":"C Yao","year":"2010","journal-title":"The American Journal of Pathology"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1007\/s10735-010-9308-0","article-title":"Localization of AQP5 during development of the mouse submandibular salivary gland","volume":"42","author":"HS Larsen","year":"2011","journal-title":"J Mol Hist"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"3621","DOI":"10.1073\/pnas.0608384104","article-title":"Interaction between transcellular and paracellular water transport pathways through aquaporin 5 and the tight junction complex","volume":"104","author":"JD Kawedia","year":"2007","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/s00232-005-0848-2","article-title":"AQP and the control of fluid transport in a salivary gland","volume":"210","author":"M Murakami","year":"2006","journal-title":"J Membr Biol"},{"key":"ref54","first-page":"2","article-title":"Radiation induced salivary gland damage: review on pathogenesis and management","volume":"4","author":"SG Kaur","year":"2016","journal-title":"Journal of Dental Sciences RRJDS"},{"issue":"3","key":"ref55","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1002\/1529-0131(200103)44:3<693::AID-ANR119>3.0.CO;2-7","article-title":"Elevated Proapoptotic Bax and Caspase 3 Activation in the NOD. Scid model of Sjogren\u2019s syndrome","volume":"44","author":"R Masago","year":"2001","journal-title":"Arthritis and Rheumatology"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1593\/neo.121528","article-title":"Prognostic Impact of Expression of Bcl-2 and Bax Genes in Circulating Immune Cells Derived from Patients with Head and Neck Carcinoma","volume":"15","author":"T Tano","year":"2013","journal-title":"Neoplasia"},{"issue":"1","key":"ref57","doi-asserted-by":"crossref","first-page":"112","DOI":"10.12816\/0015175","article-title":"BAX in situ hybridization and proliferating cell nuclear antigen immunohistochemical expressions in salivary gland tumours","volume":"26","author":"QA Hussain","year":"2014","journal-title":"J Bagh College Dentistry"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0205372","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T18:08:22Z","timestamp":1588961302000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0205372"}},"subtitle":[],"editor":[{"given":"John A.","family":"Chiorini","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2018,10,10]]},"references-count":57,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2018,10,10]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0205372","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,10]]}}}