{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T12:54:07Z","timestamp":1783947247951,"version":"3.55.0"},"reference-count":25,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2018,11,26]],"date-time":"2018-11-26T00:00:00Z","timestamp":1543190400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31600151"],"award-info":[{"award-number":["31600151"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20160130"],"award-info":[{"award-number":["BK20160130"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20160131"],"award-info":[{"award-number":["BK20160131"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Social Development Foundation of Jiangsu Province","award":["BE2016622"],"award-info":[{"award-number":["BE2016622"]}]},{"name":"China Key Project of New Medicine Development","award":["2014ZX09304314-003"],"award-info":[{"award-number":["2014ZX09304314-003"]}]},{"name":"Medical Science and Technology Projects","award":["17QNP052"],"award-info":[{"award-number":["17QNP052"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0207811","type":"journal-article","created":{"date-parts":[[2018,11,26]],"date-time":"2018-11-26T14:47:10Z","timestamp":1543243630000},"page":"e0207811","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":20,"title":["Development of a rapid and visual detection method for Rickettsia rickettsii combining recombinase polymerase assay with lateral flow test"],"prefix":"10.1371","volume":"13","author":[{"given":"Yong","family":"Qi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinxiu","family":"Shao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jixian","family":"Rao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanpeng","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiong","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoling","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongxia","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiameng","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenwen","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shulong","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suyun","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8491-4402","authenticated-orcid":true,"given":"Yuexi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2018,11,26]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1093\/cid\/ciu358","article-title":"Detection of <italic>Rickettsia rickettsii<\/italic>, <italic>Rickettsia parkeri<\/italic>, and <italic>Rickettsia akari<\/italic> in skin biopsy specimens using a multiplex real-time polymerase chain reaction assay","volume":"59","author":"AM Denison","year":"2014","journal-title":"Clinical Infectious Diseases An Official Publication of the Infectious Diseases Society of America"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1128\/CMR.00032-13","article-title":"Update on Tick-Borne Rickettsioses around the World: a Geographic Approach","volume":"26","author":"P Parola","year":"2013","journal-title":"Clinical Microbiology Reviews"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1093\/infdis\/1.1.31","article-title":"Studies in Pyroplasmosis Hominis (\u201cSpotted Fever\u201d or \u201cTick Fever\u201d of the Rocky Mountains)","volume":"1","author":"LB Wilson","year":"1904","journal-title":"Journal of Infectious Diseases"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"174","DOI":"10.4269\/ajtmh.2010.09-0752","article-title":"Rocky mountain spotted fever in the United States, 2000\u20132007: interpreting contemporary increases in incidence","volume":"83","author":"JJ Openshaw","year":"2010","journal-title":"American Journal of Tropical Medicine & Hygiene"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"713","DOI":"10.4269\/ajtmh.2012.11-0453","article-title":"Fatal Rocky Mountain spotted fever in the United States, 1999\u20132007","volume":"86","author":"FS Dahlgren","year":"2012","journal-title":"American Journal of Tropical Medicine & Hygiene"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1128\/JCM.01723-12","article-title":"Assessment of Real-Time PCR Assay for Detection of <italic>Rickettsia<\/italic> spp. and <italic>Rickettsia rickettsii<\/italic> in Banked Clinical Samples","volume":"51","author":"CY Kato","year":"2013","journal-title":"Journal of Clinical Microbiology"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1302","DOI":"10.15585\/mmwr.mm6546a6","article-title":"Notes from the Field: Community-Based Prevention of Rocky Mountain Spotted Fever\u2014Sonora, Mexico, 2016","volume":"65","author":"A Straily","year":"2016","journal-title":"Mmwr Morb Mortal Wkly Rep"},{"key":"ref8","first-page":"137","article-title":"Rocky Mountain spotted fever in children: clinical and epidemiological features","volume":"143","author":"MA Mart\u00ednez-Medina","year":"2007","journal-title":"Gaceta M\u00e9dica De M\u00e9xico"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"907","DOI":"10.4269\/ajtmh.2008.79.907","article-title":"An increase in human cases of spotted fever rickettsiosis in Yucatan, Mexico, involving children","volume":"79","author":"JE Zavalacastro","year":"2008","journal-title":"American Journal of Tropical Medicine & Hygiene"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1196\/annals.1374.030","article-title":"Brazilian spotted fever: a case series from an endemic area in southeastern Brazil: epidemiological aspects","volume":"1078","author":"RN Angerami","year":"2006","journal-title":"Annals of the New York Academy of Sciences"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1590\/S1020-49892010000600008","article-title":"A febre maculosa no Brasil","volume":"27","author":"Junqueira FM Fiol FDSD","year":"2010","journal-title":"Revista Panamericana De Salud Publica"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1590\/S0074-02762007000700003","article-title":"Rickettsia infection in five areas of the state of Sao Paulo, Brazil","volume":"102","author":"MC Horta","year":"2007","journal-title":"Mem\u00f3rias Do Instituto Oswaldo Cruz"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"e204","DOI":"10.1371\/journal.pbio.0040204","article-title":"DNA detection using recombination proteins","volume":"4","author":"O Piepenburg","year":"2006","journal-title":"PLoS biology"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1080\/21645515.2015.1011572","article-title":"<italic>Rickettsia rickettsii<\/italic> outer membrane protein YbgF induces protective immunity in C3H\/HeN mice","volume":"11","author":"W Gong","year":"2015","journal-title":"Human Vaccines"},{"key":"ref15","article-title":"Rapid detection of spotted fever group rickettsiae with real-time quantitative PCR","author":"D Niu","year":"2008","journal-title":"Medical Journal of Chinese Peoples Liberation Army"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"5643","DOI":"10.1016\/j.vaccine.2013.09.064","article-title":"Recombinant protein YbgF induces protective immunity against <italic>Rickettsia heilongjiangensis<\/italic> infection in C3H\/HeN mice","volume":"31","author":"Y Qi","year":"2013","journal-title":"Vaccine"},{"key":"ref17","first-page":"1580","article-title":"Identification of <italic>Coxiella burnetii<\/italic> CD8+ T-Cell Epitopes and Delivery by Attenuated Listeria monocytogenes as a Vaccine Vector in a C57BL\/6 Mouse Model","volume":"215","author":"X Xiong","year":"2017","journal-title":"Journal of Infectious Diseases"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"e87206","DOI":"10.1371\/journal.pone.0087206","article-title":"Exploratory study on Th1 epitope-induced protective immunity against <italic>Coxiella burnetii<\/italic> infection","volume":"9","author":"X Xiong","year":"2014","journal-title":"Plos One"},{"key":"ref19","first-page":"25","article-title":"Establishment of real-time quantitative PCR-based methods for detection of <italic>Staphylococcus aureus<\/italic> in food","volume":"34","author":"SU Yu-Xin","year":"2010","journal-title":"Bulletin of the Academy of Military Medical Sciences"},{"key":"ref20","first-page":"325","article-title":"Establishment of multiplex real-time PCR to detect highly virulent <italic>Streptococcus suis<\/italic> serotype 2","author":"J Zhu","year":"2012","journal-title":"Journal of Pathogen Biology"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.ijid.2018.03.003","article-title":"Development of a rapid and visual nucleotide detection method towards an Chinese epidemic strain of <italic>Orientia tsutsugamushi<\/italic> based on recombinase polymerase amplification assay and lateral flow test","author":"Y Qi","year":"2018","journal-title":"International Journal of Infectious Diseases"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1097\/QCO.0000000000000298","article-title":"State of the art of diagnosis of rickettsial diseases: the use of blood specimens for diagnosis of scrub typhus, spotted fever group rickettsiosis, and murine typhus","volume":"29","author":"DH Paris","year":"2016","journal-title":"Current Opinion in Infectious Diseases"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1016\/j.ttbdis.2016.05.011","article-title":"Comparative value of blood and skin samples for diagnosis of spotted fever group rickettsial infection in model animals","volume":"7","author":"ML Levin","year":"2016","journal-title":"Ticks and Tick-borne Diseases"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"1","DOI":"10.15585\/mmwr.rr6502a1","article-title":"Diagnosis and Management of Tickborne Rickettsial Diseases: Rocky Mountain Spotted Fever and Other Spotted Fever Group Rickettsioses, Ehrlichioses, and Anaplasmosis\u2014United States","volume":"65","author":"HM Biggs","year":"2016","journal-title":"MMWR Recommendations and reports: Morbidity and mortality weekly report Recommendations and reports \/ Centers for Disease Control"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"e0003884","DOI":"10.1371\/journal.pntd.0003884","article-title":"Development of Recombinase Polymerase Amplification Assays for Detection of <italic>Orientia tsutsugamushi<\/italic> or <italic>Rickettsia typhi<\/italic>","volume":"9","author":"CC Chao","year":"2015","journal-title":"Plos Negl Trop Dis"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0207811","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T20:02:56Z","timestamp":1588968176000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0207811"}},"subtitle":[],"editor":[{"given":"J. Stephen","family":"Dumler","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2018,11,26]]},"references-count":25,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2018,11,26]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0207811","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,11,26]]}}}