{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T17:38:47Z","timestamp":1775065127914,"version":"3.50.1"},"reference-count":55,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2019,1,4]],"date-time":"2019-01-04T00:00:00Z","timestamp":1546560000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24659710"],"award-info":[{"award-number":["24659710"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["26462397"],"award-info":[{"award-number":["26462397"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["17K11124"],"award-info":[{"award-number":["17K11124"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["26293349"],"award-info":[{"award-number":["26293349"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0209196","type":"journal-article","created":{"date-parts":[[2019,1,4]],"date-time":"2019-01-04T18:32:22Z","timestamp":1546626742000},"page":"e0209196","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":14,"title":["Cationized liposomal keto-mycolic acids isolated from Mycobacterium bovis bacillus Calmette-Gu\u00e9rin induce antitumor immunity in a syngeneic murine bladder cancer model"],"prefix":"10.1371","volume":"14","author":[{"given":"Takayuki","family":"Yoshino","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3618-0432","authenticated-orcid":true,"given":"Jun","family":"Miyazaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takahiro","family":"Kojima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuya","family":"Kandori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masanobu","family":"Shiga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Kawahara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomokazu","family":"Kimura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Naka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideyasu","family":"Kiyohara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miyuki","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sho","family":"Yamasaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideyuki","family":"Akaza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ikuya","family":"Yano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Nishiyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2019,1,4]]},"reference":[{"issue":"2","key":"ref1","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.eururo.2012.07.033","article-title":"Epidemiology and risk factors of urothelial bladder cancer","volume":"63","author":"M Burger","year":"2013","journal-title":"Eur Urol"},{"issue":"3","key":"ref2","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/j.eururo.2016.05.041","article-title":"EAU Guidelines on Non-Muscle-invasive Urothelial Carcinoma of the Bladder: Update 2016","volume":"71","author":"M Babjuk","year":"2017","journal-title":"Eur Urol"},{"issue":"2","key":"ref3","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/S0022-5347(17)58737-6","article-title":"Intracavitary Bacillus Calmette-Guerin in the treatment of superficial bladder tumors","volume":"116","author":"A Morales","year":"1976","journal-title":"J Urol"},{"issue":"6","key":"ref4","doi-asserted-by":"crossref","first-page":"2004","DOI":"10.1016\/S0022-5347(06)00264-3","article-title":"Complications of intravesical therapy for urothelial cancer of the bladder","volume":"175","author":"MP Koya","year":"2006","journal-title":"J Urol"},{"issue":"8","key":"ref5","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1093\/jjco\/hyt086","article-title":"Adverse reactions related to treatment compliance during BCG maintenance therapy for non-muscle-invasive bladder cancer","volume":"43","author":"J Miyazaki","year":"2013","journal-title":"Jpn J Clin Oncol"},{"issue":"3","key":"ref6","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1038\/nrurol.2014.15","article-title":"The mechanism of action of BCG therapy for bladder cancer\u2014a current perspective","volume":"11","author":"G Redelman-Sidi","year":"2014","journal-title":"Nature reviews Urology"},{"issue":"1","key":"ref7","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1111\/cas.12075","article-title":"Bacillus Calmette-Guerin (BCG) immunotherapy for bladder cancer: current understanding and perspectives on engineered BCG vaccine","volume":"104","author":"K Kawai","year":"2013","journal-title":"Cancer science"},{"issue":"5","key":"ref8","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/S1078-1439(00)00074-0","article-title":"Glycosaminoglycan content of human bladders. a method of analysis using cold-cup biopsies","volume":"5","author":"MM Poggi","year":"2000","journal-title":"Urol Oncol"},{"issue":"3","key":"ref9","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1002\/ijc.26413","article-title":"Fibronectin attachment protein from bacillus Calmette-Guerin as targeting agent for bladder tumor cells","volume":"131","author":"BG Coon","year":"2012","journal-title":"Int J Cancer"},{"issue":"2","key":"ref10","first-page":"181","article-title":"BCG-induced interleukin-6 upregulation and BCG internalization in well and poorly differentiated human bladder cancer cell lines","volume":"9","author":"RF Bevers","year":"1998","journal-title":"Eur Cytokine Netw"},{"issue":"5","key":"ref11","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1007\/BF00296835","article-title":"Cytokine production by the human bladder carcinoma cell line T24 in the presence of bacillus Calmette-Guerin (BCG)","volume":"21","author":"TM de Reijke","year":"1993","journal-title":"Urol Res"},{"issue":"2","key":"ref12","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/S1286-4579(00)00268-9","article-title":"Bacterial modulation of antigen processing and presentation","volume":"2","author":"WP Maksymowych","year":"2000","journal-title":"Microbes Infect"},{"issue":"5","key":"ref13","doi-asserted-by":"crossref","first-page":"1793","DOI":"10.1128\/IAI.65.5.1793-1799.1997","article-title":"In vivo induction of apoptosis in the thymus by administration of mycobacterial cord factor (trehalose 6,6&apos;-dimycolate)","volume":"65","author":"Y Ozeki","year":"1997","journal-title":"Infection and immunity"},{"issue":"2","key":"ref14","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1128\/IAI.69.2.810-815.2001","article-title":"Trehalose 6,6&apos;-dimycolate (cord factor) of Mycobacterium tuberculosis induces foreign-body- and hypersensitivity-type granulomas in mice","volume":"69","author":"H Yamagami","year":"2001","journal-title":"Infection and immunity"},{"issue":"9","key":"ref15","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1016\/S1286-4579(99)80072-0","article-title":"Roles of lipoarabinomannan in the pathogenesis of tuberculosis","volume":"1","author":"GR Strohmeier","year":"1999","journal-title":"Microbes Infect"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.jconrel.2013.12.027","article-title":"Nanoparticulation of BCG-CWS for application to bladder cancer therapy","volume":"176","author":"T Nakamura","year":"2014","journal-title":"Journal of controlled release: official journal of the Controlled Release Society"},{"issue":"11","key":"ref17","doi-asserted-by":"crossref","first-page":"3973","DOI":"10.1128\/AEM.59.11.3973-3977.1993","article-title":"Physicochemical cell surface and adhesive properties of coryneform bacteria related to the presence and chain length of mycolic acids","volume":"59","author":"B Bendinger","year":"1993","journal-title":"Applied and environmental microbiology"},{"issue":"3","key":"ref18","doi-asserted-by":"crossref","first-page":"1418","DOI":"10.1128\/jb.172.3.1418-1423.1990","article-title":"Permeability barrier to hydrophilic solutes in Mycobacterium chelonei","volume":"172","author":"V Jarlier","year":"1990","journal-title":"Journal of bacteriology"},{"issue":"1","key":"ref19","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.chembiol.2013.11.011","article-title":"Mycolic acids: structures, biosynthesis, and beyond","volume":"21","author":"H Marrakchi","year":"2014","journal-title":"Chem Biol"},{"issue":"1","key":"ref20","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1093\/jnci\/52.1.95","article-title":"Biologically active components from mycobacterial cell walls. I. Isolation and composition of cell wall skeleton and component P3","volume":"52","author":"I Azuma","year":"1974","journal-title":"J Natl Cancer Inst"},{"issue":"6507","key":"ref21","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1038\/372691a0","article-title":"Recognition of a lipid antigen by CD1-restricted alpha beta+ T cells","volume":"372","author":"EM Beckman","year":"1994","journal-title":"Nature"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1146\/annurev.immunol.22.012703.104608","article-title":"CD1: antigen presentation and T cell function","volume":"22","author":"M Brigl","year":"2004","journal-title":"Annu Rev Immunol"},{"issue":"9","key":"ref23","doi-asserted-by":"crossref","first-page":"1525","DOI":"10.1002\/eji.201747062","article-title":"CD1b-mycolic acid tetramers demonstrate T-cell fine specificity for mycobacterial lipid tails","volume":"47","author":"I Van Rhijn","year":"2017","journal-title":"European Journal of Immunology"},{"issue":"3","key":"ref24","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1002\/eji.200425332","article-title":"The Mycobacterium tuberculosis cell wall component mycolic acid elicits pathogen-associated host innate immune responses","volume":"35","author":"J Korf","year":"2005","journal-title":"Eur J Immunol"},{"issue":"2","key":"ref25","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1002\/eji.201040719","article-title":"Molecular structure of the Mycobacterium tuberculosis virulence factor, mycolic acid, determines the elicited inflammatory pattern","volume":"41","author":"S Vander Beken","year":"2011","journal-title":"Eur J Immunol"},{"issue":"Pt 10","key":"ref26","doi-asserted-by":"crossref","first-page":"3403","DOI":"10.1099\/mic.0.28158-0","article-title":"Intact molecular characterization of cord factor (trehalose 6,6&apos;-dimycolate) from nine species of mycobacteria by MALDI-TOF mass spectrometry","volume":"151","author":"Y Fujita","year":"2005","journal-title":"Microbiology"},{"issue":"10","key":"ref27","doi-asserted-by":"crossref","first-page":"3967","DOI":"10.1073\/pnas.0611618104","article-title":"CD8 tumor-infiltrating lymphocytes are predictive of survival in muscle-invasive urothelial carcinoma","volume":"104","author":"P Sharma","year":"2007","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"2","key":"ref28","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.mimet.2007.11.016","article-title":"Comprehensive analysis of mycolic acid subclass and molecular species composition of Mycobacterium bovis BCG Tokyo 172 cell wall skeleton (SMP-105)","volume":"72","author":"Y Uenishi","year":"2008","journal-title":"Journal of microbiological methods"},{"issue":"2","key":"ref29","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.bbamem.2005.07.005","article-title":"Temperature dependence of the Langmuir monolayer packing of mycolic acids from Mycobacterium tuberculosis","volume":"1715","author":"M Villeneuve","year":"2005","journal-title":"Biochim Biophys Acta"},{"issue":"7","key":"ref30","doi-asserted-by":"crossref","first-page":"1717","DOI":"10.1016\/j.bbamem.2007.04.003","article-title":"Conformational behavior of oxygenated mycobacterial mycolic acids from Mycobacterium bovis BCG","volume":"1768","author":"M Villeneuve","year":"2007","journal-title":"Biochim Biophys Acta"},{"issue":"6","key":"ref31","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1016\/j.chemphyslip.2010.04.010","article-title":"Differential conformational behaviors of alpha-mycolic acids in Langmuir monolayers and computer simulations","volume":"163","author":"M Villeneuve","year":"2010","journal-title":"Chemistry and physics of lipids"},{"issue":"Pt 11","key":"ref32","doi-asserted-by":"crossref","first-page":"2405","DOI":"10.1099\/mic.0.068866-0","article-title":"Conformational folding of mycobacterial methoxy- and ketomycolic acids facilitated by alpha-methyl trans-cyclopropane groups rather than cis-cyclopropane units","volume":"159","author":"M Villeneuve","year":"2013","journal-title":"Microbiology"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.chemphyslip.2013.12.004","article-title":"Differential spontaneous folding of mycolic acids from Mycobacterium tuberculosis","volume":"180","author":"W Groenewald","year":"2014","journal-title":"Chemistry and physics of lipids"},{"key":"ref34","article-title":"Pathophysiological implications of cell envelope structure in Mycobacterium tuberculosis and related taxa","author":"DE Minnikin","year":"2015","journal-title":"Intech"},{"issue":"1","key":"ref35","doi-asserted-by":"crossref","first-page":"295","DOI":"10.4049\/jimmunol.1400092","article-title":"Mycobacterium tuberculosis keto-mycolic acid and macrophage nuclear receptor TR4 modulate foamy biogenesis in granulomas: a case of a heterologous and noncanonical ligand-receptor pair","volume":"193","author":"HK Dkhar","year":"2014","journal-title":"J Immunol"},{"issue":"7","key":"ref36","doi-asserted-by":"crossref","first-page":"4590","DOI":"10.4049\/jimmunol.173.7.4590","article-title":"Activation of CD8 T cells by mycobacterial vaccination protects against pulmonary tuberculosis in the absence of CD4 T cells","volume":"173","author":"J Wang","year":"2004","journal-title":"J Immunol"},{"issue":"1","key":"ref37","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.it.2016.10.009","article-title":"Recognition of Mycobacterial Lipids by Immune Receptors","volume":"38","author":"E Ishikawa","year":"2017","journal-title":"Trends in immunology"},{"issue":"4","key":"ref38","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1038\/nrmicro2321","article-title":"Regulation of antigen presentation by Mycobacterium tuberculosis: a role for Toll-like receptors","volume":"8","author":"CV Harding","year":"2010","journal-title":"Nature reviews Microbiology"},{"issue":"3","key":"ref39","doi-asserted-by":"crossref","first-page":"1018","DOI":"10.1016\/S0022-5347(17)35678-1","article-title":"T-cell subsets required for intravesical BCG immunotherapy for bladder cancer","volume":"150","author":"TL Ratliff","year":"1993","journal-title":"J Urol"},{"issue":"1","key":"ref40","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/S0022-5347(17)43909-7","article-title":"Requirement of a thymus dependent immune response for BCG-mediated antitumor activity","volume":"137","author":"TL Ratliff","year":"1987","journal-title":"J Urol"},{"issue":"6","key":"ref41","first-page":"1469","article-title":"Significance of target cell infection and natural killer cells in the anti-tumor effects of bacillus Calmette-Guerin in murine bladder cancer","volume":"17","author":"T Sonoda","year":"2007","journal-title":"Oncol Rep"},{"issue":"5","key":"ref42","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1002\/1097-0215(20010601)92:5<697::AID-IJC1245>3.0.CO;2-Z","article-title":"NK cells are essential for effective BCG immunotherapy","volume":"92","author":"S Brandau","year":"2001","journal-title":"Int J Cancer"},{"issue":"6","key":"ref43","doi-asserted-by":"crossref","first-page":"1589","DOI":"10.1016\/j.eururo.2006.11.045","article-title":"The role of granulocytes following intravesical BCG prophylaxis","volume":"51","author":"S Siracusano","year":"2007","journal-title":"Eur Urol"},{"issue":"16","key":"ref44","doi-asserted-by":"crossref","first-page":"8250","DOI":"10.1158\/0008-5472.CAN-06-1416","article-title":"Neutrophil granulocytes are required for effective Bacillus Calmette-Guerin immunotherapy of bladder cancer and orchestrate local immune responses","volume":"66","author":"H Suttmann","year":"2006","journal-title":"Cancer Res"},{"issue":"4","key":"ref45","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1038\/bjc.1995.155","article-title":"Bacillus Calmette-Guerin (BCG) enhances monocyte- and lymphocyte-mediated bladder tumour cell killing","volume":"71","author":"K Pryor","year":"1995","journal-title":"Br J Cancer"},{"issue":"5","key":"ref46","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1111\/j.1349-7006.2000.tb00978.x","article-title":"Enhancing effect of an inhibitor of nitric oxide synthesis on bacillus Calmette-Guerin-induced macrophage cytotoxicity against murine bladder cancer cell line MBT-2 in vitro","volume":"91","author":"H Yamada","year":"2000","journal-title":"Jpn J Cancer Res"},{"issue":"6","key":"ref47","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1111\/j.1442-2042.2006.01697.x","article-title":"Bacillus Calmette-Guerin-pulsed dendritic cells stimulate natural killer T cells and gammadeltaT cells","volume":"14","author":"M Naoe","year":"2007","journal-title":"Int J Urol"},{"issue":"8","key":"ref48","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1007\/s00262-008-0643-x","article-title":"A possible mechanism of intravesical BCG therapy for human bladder carcinoma: involvement of innate effector cells for the inhibition of tumor growth","volume":"58","author":"T Higuchi","year":"2009","journal-title":"Cancer immunology, immunotherapy: CII"},{"issue":"1","key":"ref49","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1046\/j.1442-2042.2002.00415.x","article-title":"Surface antigen expression on bladder tumor cells induced by bacillus Calmette-Guerin (BCG): A role of BCG internalization into tumor cells","volume":"9","author":"N Ikeda","year":"2002","journal-title":"Int J Urol"},{"issue":"5","key":"ref50","doi-asserted-by":"crossref","first-page":"1553","DOI":"10.1016\/S0022-5347(05)67678-1","article-title":"Autocrine IL-6 production by human transitional carcinoma cells upregulates expression of the alpha5beta1 firbonectin receptor","volume":"163","author":"GJ Zhang","year":"2000","journal-title":"J Urol"},{"issue":"4","key":"ref51","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1111\/j.1464-410X.2006.06026.x","article-title":"Uroepithelial cells can directly respond to Mycobacterium bovis bacillus Calmette-Guerin through Toll-like receptor signalling","volume":"97","author":"J Miyazaki","year":"2006","journal-title":"BJU Int"},{"issue":"00","key":"ref52","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1111\/j.1749-6632.1976.tb41700.x","article-title":"Immunotherapy with nonviable microbial components","volume":"277","author":"E Ribi","year":"1976","journal-title":"Annals of the New York Academy of Sciences"},{"issue":"6","key":"ref53","first-page":"2065","article-title":"The therapeutic effects of R8-liposome-BCG-CWS on BBN-induced rat urinary bladder carcinoma","volume":"31","author":"J Miyazaki","year":"2011","journal-title":"Anticancer Res"},{"issue":"9","key":"ref54","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1093\/intimm\/dxx051","article-title":"Anti-tumor immunity via the superoxide-eosinophil axis induced by a lipophilic component of Mycobacterium lipomannan","volume":"29","author":"T Ito","year":"2017","journal-title":"International immunology"},{"issue":"4","key":"ref55","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1177\/0885328211411955","article-title":"Polyamidoamine dendron-bearing lipid assemblies: their morphologies and gene transfection ability","volume":"27","author":"S Iwashita","year":"2012","journal-title":"Journal of biomaterials applications"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0209196","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,21]],"date-time":"2020-11-21T17:59:30Z","timestamp":1605981570000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0209196"}},"subtitle":[],"editor":[{"given":"Abdelwahab","family":"Omri","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2019,1,4]]},"references-count":55,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2019,1,4]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0209196","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,1,4]]}}}