{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T12:44:35Z","timestamp":1769085875681,"version":"3.49.0"},"reference-count":41,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2018,12,31]],"date-time":"2018-12-31T00:00:00Z","timestamp":1546214400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Youth Start-up Fund of the Shanghai Changhai Hospital (CN)","award":["CH201805"],"award-info":[{"award-number":["CH201805"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0210087","type":"journal-article","created":{"date-parts":[[2018,12,31]],"date-time":"2018-12-31T18:35:43Z","timestamp":1546281343000},"page":"e0210087","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":6,"title":["Identification of suitable reference genes for real-time qPCR in homocysteine-treated human umbilical vein endothelial cells"],"prefix":"10.1371","volume":"13","author":[{"given":"Xia","family":"Zhu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lujun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yangxi","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4445-9596","authenticated-orcid":true,"given":"Jianliang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2018,12,31]]},"reference":[{"issue":"5","key":"ref1","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1007\/s00401-016-1571-z","article-title":"Neuropathological diagnosis of vascular cognitive impairment and vascular dementia with implications for Alzheimer&apos;s disease","volume":"131","author":"RN Kalaria","year":"2016","journal-title":"Acta neuropathologica"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/1475-2891-14-6","article-title":"Role of homocysteine in the development of cardiovascular disease","volume":"14","author":"P Ganguly","year":"2015","journal-title":"Nutrition journal"},{"issue":"1","key":"ref3","doi-asserted-by":"crossref","first-page":"6316","DOI":"10.1038\/s41598-017-06611-3","article-title":"Associations of plasma homocysteine levels with peripheral systolic blood pressure and noninvasive central systolic blood pressure in a community-based Chinese population","volume":"7","author":"M Momin","year":"2017","journal-title":"Scientific reports"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.biopha.2016.02.022","article-title":"Alpha-lipoic acid defends homocysteine-induced endoplasmic reticulum and oxidative stress in HAECs","volume":"80","author":"H Hu","year":"2016","journal-title":"Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie"},{"issue":"8","key":"ref5","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1111\/bph.13988","article-title":"Hyperhomocysteinaemia and vascular injury: advances in mechanisms and drug targets","volume":"175","author":"Y Fu","year":"2018","journal-title":"Br J Pharmacol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"5736506","DOI":"10.1155\/2017\/5736506","article-title":"Homocysteine Induces Apoptosis of Human Umbilical Vein Endothelial Cells via Mitochondrial Dysfunction and Endoplasmic Reticulum Stress","volume":"2017","author":"Z Zhang","year":"2017","journal-title":"Oxidative medicine and cellular longevity"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.aquatox.2017.07.009","article-title":"Expression stability and selection of optimal reference genes for gene expression normalization in early life stage rainbow trout exposed to cadmium and copper","volume":"190","author":"K Shekh","year":"2017","journal-title":"Aquatic toxicology (Amsterdam, Netherlands)"},{"issue":"1","key":"ref8","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.jneumeth.2009.12.018","article-title":"Selection of reference genes for gene expression studies in rat oligodendrocytes using quantitative real time PCR","volume":"187","author":"K Nelissen","year":"2010","journal-title":"Journal of neuroscience methods"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.mimet.2017.07.011","article-title":"Selection of reliable reference genes for gene expression studies in Trichoderma afroharzianum LTR-2 under oxalic acid stress","volume":"141","author":"Y Lyu","year":"2017","journal-title":"Journal of microbiological methods"},{"issue":"3","key":"ref10","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1016\/j.thromres.2015.07.005","article-title":"Differential expression of plasma miRNAs in patients with unprovoked venous thromboembolism and healthy control individuals","volume":"136","author":"I Starikova","year":"2015","journal-title":"Thromb Res"},{"issue":"10","key":"ref11","doi-asserted-by":"crossref","first-page":"e47510","DOI":"10.1371\/journal.pone.0047510","article-title":"Housekeeping gene selection advisory: glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and beta-actin are targets of miR-644a","volume":"7","author":"K Sikand","year":"2012","journal-title":"PloS one"},{"issue":"3","key":"ref12","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1007\/s12013-009-9063-6","article-title":"MicroRNAs are involved in homocysteine-induced cardiac remodeling","volume":"55","author":"PK Mishra","year":"2009","journal-title":"Cell Biochem Biophys"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"35302","DOI":"10.1038\/srep35302","article-title":"miRNA-145 inhibits VSMC proliferation by targeting CD40","volume":"6","author":"X Guo","year":"2016","journal-title":"Scientific reports"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1186\/1471-2199-9-53","article-title":"Selection of reference genes for quantitative real-time PCR in a rat asphyxial cardiac arrest model","volume":"9","author":"K Langnaese","year":"2008","journal-title":"BMC molecular biology"},{"issue":"5","key":"ref15","doi-asserted-by":"crossref","first-page":"e36323","DOI":"10.1371\/journal.pone.0036323","article-title":"Reference genes for real-time PCR quantification of microRNAs and messenger RNAs in rat models of hepatotoxicity","volume":"7","author":"MN Lardizabal","year":"2012","journal-title":"PloS one"},{"issue":"4","key":"ref16","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1373\/clinchem.2008.112797","article-title":"The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments","volume":"55","author":"SA Bustin","year":"2009","journal-title":"Clinical chemistry"},{"issue":"7","key":"ref17","doi-asserted-by":"crossref","first-page":"Research0034","DOI":"10.1186\/gb-2002-3-7-research0034","article-title":"Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes","volume":"3","author":"J Vandesompele","year":"2002","journal-title":"Genome biology"},{"issue":"15","key":"ref18","doi-asserted-by":"crossref","first-page":"5245","DOI":"10.1158\/0008-5472.CAN-04-0496","article-title":"Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets","volume":"64","author":"CL Andersen","year":"2004","journal-title":"Cancer research"},{"issue":"6","key":"ref19","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1023\/B:BILE.0000019559.84305.47","article-title":"Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper\u2014Excel-based tool using pair-wise correlations","volume":"26","author":"MW Pfaffl","year":"2004","journal-title":"Biotechnology letters"},{"issue":"7","key":"ref20","doi-asserted-by":"crossref","first-page":"e0181862","DOI":"10.1371\/journal.pone.0181862","article-title":"Selection and validation of reference genes for quantitative real-time PCR analysis under different experimental conditions in the leafminer Liriomyza trifolii (Diptera: Agromyzidae)","volume":"12","author":"YW Chang","year":"2017","journal-title":"PloS one"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/1471-2199-7-33","article-title":"Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR","volume":"7","author":"N Silver","year":"2006","journal-title":"BMC molecular biology"},{"issue":"12","key":"ref22","doi-asserted-by":"crossref","first-page":"21825","DOI":"10.3390\/ijms151221825","article-title":"Real-time qPCR identifies suitable reference genes for Borna disease virus-infected rat cortical neurons","volume":"15","author":"L Zhang","year":"2014","journal-title":"International journal of molecular sciences"},{"issue":"14","key":"ref23","doi-asserted-by":"crossref","first-page":"2846","DOI":"10.7150\/jca.19897","article-title":"ERCC1_202 Is A Prognostic Biomarker in Advanced Stage Non-Small Cell Lung Cancer Patients Treated with Platinum-Based Chemotherapy","volume":"8","author":"X Wang","year":"2017","journal-title":"Journal of Cancer"},{"issue":"6","key":"ref24","doi-asserted-by":"crossref","first-page":"2207","DOI":"10.1159\/000479995","article-title":"Mir-21 Promotes Cardiac Fibrosis After Myocardial Infarction Via Targeting Smad7","volume":"42","author":"J Yuan","year":"2017","journal-title":"Cellular physiology and biochemistry: international journal of experimental cellular physiology, biochemistry, and pharmacology"},{"key":"ref25","doi-asserted-by":"crossref","DOI":"10.1139\/gen-2017-0176","article-title":"Selecting and Validating Reference Genes for Quantitative Real-time PCR in Plutella xylostella (L.)","author":"Y You","year":"2018","journal-title":"Genome"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"75","DOI":"10.3389\/fgene.2018.00075","article-title":"Evaluation of Reference Genes to Analyze Gene Expression in Silverside Odontesthes humensis Under Different Environmental Conditions","volume":"9","author":"TLR Silveira","year":"2018","journal-title":"Front Genet"},{"issue":"3","key":"ref27","doi-asserted-by":"crossref","first-page":"e0191558","DOI":"10.1371\/journal.pone.0191558","article-title":"Selection of suitable reference genes for normalization of quantitative RT-PCR (RT-qPCR) expression data across twelve tissues of riverine buffaloes (Bubalus bubalis)","volume":"13","author":"R Kaur","year":"2018","journal-title":"PloS one"},{"issue":"4","key":"ref28","doi-asserted-by":"crossref","first-page":"e0195096","DOI":"10.1371\/journal.pone.0195096","article-title":"Screening potential reference genes for quantitative real-time PCR analysis in the oriental armyworm, Mythimna separata","volume":"13","author":"HB Li","year":"2018","journal-title":"PloS one"},{"key":"ref29","article-title":"LncRNA SNHG1 functions as a ceRNA to antagonize the effect of miR-145a-5p on the down-regulation of NUAK1 in nasopharyngeal carcinoma cell","author":"X Lan","year":"2018","journal-title":"Journal of cellular and molecular medicine"},{"issue":"3","key":"ref30","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1517\/14728222.2013.745512","article-title":"Overexpression of microRNA-145 in atherosclerotic plaques from hypertensive patients","volume":"17","author":"D Santovito","year":"2013","journal-title":"Expert opinion on therapeutic targets"},{"issue":"5","key":"ref31","first-page":"6115","article-title":"Expression of the microRNA-143\/145 cluster is decreased in hepatitis B virus-associated hepatocellular carcinoma and may serve as a biomarker for tumorigenesis in patients with chronic hepatitis B","volume":"15","author":"Q Zhao","year":"2018","journal-title":"Oncology letters"},{"issue":"4","key":"ref32","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1160\/TH13-11-0905","article-title":"MiR-143\/145 deficiency attenuates the progression of atherosclerosis in Ldlr-\/-mice","volume":"112","author":"F Sala","year":"2014","journal-title":"Thrombosis and haemostasis"},{"issue":"3","key":"ref33","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1161\/CIRCRESAHA.112.267591","article-title":"A role for miR-145 in pulmonary arterial hypertension: evidence from mouse models and patient samples","volume":"111","author":"P Caruso","year":"2012","journal-title":"Circ Res"},{"issue":"1","key":"ref34","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1007\/s00395-010-0123-2","article-title":"MicroRNA signatures in total peripheral blood as novel biomarkers for acute myocardial infarction","volume":"106","author":"B Meder","year":"2011","journal-title":"Basic research in cardiology"},{"issue":"1","key":"ref35","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1186\/s12872-017-0596-0","article-title":"Expression levels of atherosclerosis-associated miR-143 and miR-145 in the plasma of patients with hyperhomocysteinaemia","volume":"17","author":"K Liu","year":"2017","journal-title":"BMC cardiovascular disorders"},{"issue":"1","key":"ref36","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/jcb.23341","article-title":"A microRNA &quot;target pools&quot; remains mysterious","volume":"113","author":"N Ma","year":"2012","journal-title":"Journal of cellular biochemistry"},{"issue":"5","key":"ref37","first-page":"848","article-title":"Beta-actin cannot be used as a control for gene expression in ovine interstitial cells derived from heart valves","volume":"13","author":"J Yperman","year":"2004","journal-title":"The Journal of heart valve disease"},{"issue":"1","key":"ref38","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1093\/carcin\/bgs354","article-title":"beta-Actin is predicted as one of the potential targets of miR-145: choose internal control gene in verification of microRNA target","volume":"34","author":"N Ma","year":"2013","journal-title":"Carcinogenesis"},{"issue":"11","key":"ref39","doi-asserted-by":"crossref","first-page":"3810","DOI":"10.1096\/fj.201600539","article-title":"N-Homocysteinylation impairs collagen cross-linking in cystathionine beta-synthase-deficient mice: a novel mechanism of connective tissue abnormalities","volume":"30","author":"J Perla-Kajan","year":"2016","journal-title":"FASEB J"},{"issue":"7","key":"ref40","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.1007\/s00726-015-1956-7","article-title":"Homocysteine thiolactone and N-homocysteinylated protein induce pro-atherogenic changes in gene expression in human vascular endothelial cells","volume":"47","author":"D Gurda","year":"2015","journal-title":"Amino Acids"},{"issue":"1","key":"ref41","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1161\/01.RES.87.1.45","article-title":"Homocysteine thiolactone and protein homocysteinylation in human endothelial cells: implications for atherosclerosis","volume":"87","author":"H Jakubowski","year":"2000","journal-title":"Circ Res"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0210087","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,9]],"date-time":"2020-05-09T07:24:48Z","timestamp":1589009088000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0210087"}},"subtitle":[],"editor":[{"given":"Michela","family":"Grosso","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2018,12,31]]},"references-count":41,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2018,12,31]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0210087","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,12,31]]}}}