{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T03:27:48Z","timestamp":1778988468231,"version":"3.51.4"},"reference-count":53,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2019,2,7]],"date-time":"2019-02-07T00:00:00Z","timestamp":1549497600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010198","name":"Ministerio de Econom\u00eda, Industria y Competitividad, Gobierno de Espa\u00f1a","doi-asserted-by":"publisher","award":["AGL 2014-57752-C2-1-R"],"award-info":[{"award-number":["AGL 2014-57752-C2-1-R"]}],"id":[{"id":"10.13039\/501100010198","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010198","name":"Ministerio de Econom\u00eda, Industria y Competitividad, Gobierno de Espa\u00f1a","doi-asserted-by":"publisher","award":["AGL2017-83498-C2-1-R"],"award-info":[{"award-number":["AGL2017-83498-C2-1-R"]}],"id":[{"id":"10.13039\/501100010198","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0210545","type":"journal-article","created":{"date-parts":[[2019,2,7]],"date-time":"2019-02-07T19:19:35Z","timestamp":1549567175000},"page":"e0210545","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":11,"title":["Quantification of dose-mortality responses in adult Diptera: Validation using Ceratitis capitata and Drosophila suzukii responses to spinosad"],"prefix":"10.1371","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3965-7424","authenticated-orcid":true,"given":"Daniel","family":"Valtierra-de-Luis","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3894-0060","authenticated-orcid":true,"given":"Maite","family":"Villanueva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javier","family":"Caballero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabel M.","family":"Matas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3414-3440","authenticated-orcid":true,"given":"Trevor","family":"Williams","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Primitivo","family":"Caballero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2019,2,7]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","DOI":"10.1201\/9781315373775","article-title":"Bioassays with arthropods","author":"JL Robertson","year":"2017"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1093\/jee\/87.5.1141","article-title":"Insecticide resistance in European and African strains of western flower thrips (Thysanoptera: Thripidae) tested in a new residue-on-glass test","volume":"87","author":"HF Brodsgaard","year":"1994","journal-title":"J Econ Entomol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/jee\/72.1.1","article-title":"Dosage-mortality studies of synthetic pyrethroids and methyl parathion on the tobacco budworm in central Arizona","volume":"72","author":"LA Crowder","year":"1979","journal-title":"J Econ Entomol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1111\/j.1570-7458.1981.tb03589.x","article-title":"Effects of fenvalerate and azinphosmethyl on two-spotted spider mite and phytoseiid mites","volume":"30","author":"DR Penman","year":"1981","journal-title":"Ent Exp & Appl"},{"key":"ref5","first-page":"39","article-title":"Pesticide resistance in arthropods","author":"JG Scott","year":"1990"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1007\/s001289900244","article-title":"Analytical techniques for quantifying direct, residual, and oral exposure of an insect parasitoid to an organophosphate insecticide","volume":"57","author":"M Longley","year":"1996","journal-title":"Bull Environ Contam Toxicol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1002\/9783527644179.ch27","article-title":"IRAC: insecticide resistance, and mode of action classification of insecticides","volume":"3","author":"R Nauen","year":"2012","journal-title":"Modern Crop Protection Compounds"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1146\/annurev-ento-120811-153653","article-title":"The adult dipteran crop: a unique and overlooked organ","volume":"58","author":"JG Stoffolano Jr","year":"2013","journal-title":"Annu Rev Entomol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"e98949","DOI":"10.1371\/journal.pone.0098949","article-title":"Erythritol, a non-nutritive sugar alcohol sweetener and the main component of Truvia\u00ae, is a palatable ingested insecticide","volume":"9","author":"KM Baudier","year":"2014","journal-title":"PLoS ONE"},{"key":"ref10","unstructured":"EPPO. European and Mediterranean Plant Protection Organization, A2 List of pests recommended for regulation as quarantine pests. [cited 2018 Dec 19] Available from: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/www.eppo.int\/ACTIVITIES\/plant_quarantine\/A2_list\" xlink:type=\"simple\">https:\/\/www.eppo.int\/ACTIVITIES\/plant_quarantine\/A2_list<\/ext-link>"},{"key":"ref11","article-title":"Fruit fly pests: a world assessment of their biology and management","author":"BA McPheron","year":"1996"},{"key":"ref12","first-page":"149","article-title":"A review of the invasion of <italic>Drosophila suzukii<\/italic> in Europe and a draft research agenda for integrated pest management","volume":"65","author":"A Cini","year":"2012","journal-title":"Bull Insectol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1093\/jee\/92.1.156","article-title":"field evaluation of female-targeted trapping systems for <italic>Ceratitis capitata<\/italic> (Diptera: Tephritidae) in Seven Countries","volume":"92","author":"ND Epsky","year":"1999","journal-title":"J Econ Entomol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1002\/ps.3393","article-title":"Efficacy of attract-and-kill devices for the control of <italic>Ceratitis capitata<\/italic>","volume":"69","author":"V Navarro-Llopis","year":"2013","journal-title":"Pest Manag Sci"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.cropro.2013.08.003","article-title":"Control of spotted wing drosophila, <italic>Drosophila suzukii<\/italic>, by specific insecticides and by conventional and organic crop protection programs","volume":"54","author":"S Van Timmeren","year":"2013","journal-title":"Crop Protection"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"e0188350","DOI":"10.1371\/journal.pone.0188350","article-title":"Improved capture of <italic>Drosophila suzukii<\/italic> by a trap baited with two attractants in the same device","volume":"12","author":"R Lasa","year":"2017","journal-title":"PLoS ONE"},{"key":"ref17","article-title":"Sterile insect technique: principles and practice in area-wide integrated pest management","author":"VA Dyck","year":"2006"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1002\/(SICI)1096-9063(199611)48:3<261::AID-PS467>3.0.CO;2-R","article-title":"Laboratory evaluation of the novel naturally derived compound spinosad against <italic>Ceratitis capitata<\/italic>","volume":"48","author":"A Adan","year":"1996","journal-title":"Pest Manag Sci"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"672","DOI":"10.2307\/3496400","article-title":"Efficacy of spinosad bait sprays to control Mediterranean and Caribbean fruit flies","volume":"84","author":"RE Burns","year":"2001","journal-title":"Florida Entomol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1093\/jee\/99.2.359","article-title":"Insecticidal effects of various concentrations of selected extractions of <italic>Cestrum parqui<\/italic> on adult and immature <italic>Ceratitis capitata<\/italic>","volume":"99","author":"N Zapata","year":"2006","journal-title":"J Econ Entomol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1093\/jee\/18.2.265a","article-title":"A method of computing the effectiveness of an insecticide","volume":"18","author":"WS Abbott","year":"1925","journal-title":"J Econ Entomol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1603\/0022-0493-93.2.280","article-title":"Field tests of environmentally friendly malathion replacements to suppress wild Mediterranean fruit fly (Diptera: Tephritidae)","volume":"93","author":"SL Peck","year":"2000","journal-title":"J Econ Entomol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.cropro.2017.09.010","article-title":"Laboratory and field trials to identify effective chemical control strategies for integrated management of <italic>Drosophila suzukii<\/italic> in European cherry orchards","volume":"103","author":"R Shawer","year":"2018","journal-title":"Crop Protection"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.1002\/ps.2242","article-title":"Laboratory and field comparisons of insecticides to reduce infestation of <italic>Drosophila suzukii<\/italic> in berry crops","volume":"67","author":"DJ Bruck","year":"2011","journal-title":"Pest Manag Sci"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"G1","DOI":"10.1603\/IPM10010","article-title":"<italic>Drosophila suzukii<\/italic> (Diptera: Drosophilidae): invasive pest of ripening soft fruit expanding its geographic range and damage potential","volume":"2","author":"DB Walsh","year":"2011","journal-title":"J Integr Pest Manag"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1080\/0958315031000140956","article-title":"Is the naturally derived insecticide Spinosad\u00ae compatible with insect natural enemies?","volume":"13","author":"T Williams","year":"2003","journal-title":"Biocontrol Sci Tech"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1093\/jee\/97.3.911","article-title":"Toxicity of spinosad in protein bait to three economically important tephritid fruit fly species (Diptera: Tephritidae) and their parasitoids (Hymenoptera: Braconidae)","volume":"97","author":"JD Stark","year":"2004","journal-title":"J Econ Entomol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1007\/s10340-017-0913-5","article-title":"Susceptibility of <italic>Ceratitis capitata<\/italic> to deltamethrin and spinosad in Greece","volume":"91","author":"CC Voudouris","year":"2017","journal-title":"J Pest Sci"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"73","DOI":"10.3390\/insects9030073","article-title":"Sex-specific sub-lethal effects and immune response in <italic>Ceratitis capitata<\/italic> wied. (Diptera: Tephritidae) challenged with Spinosad","volume":"9","author":"ME Mura","year":"2018","journal-title":"Insects"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"1386","DOI":"10.1002\/ps.2279","article-title":"Developing <italic>Drosophila suzukii<\/italic> management programs for sweet cherry in the western United States","volume":"67","author":"EH Beers","year":"2011","journal-title":"Pest Manag Sci"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"952","DOI":"10.3390\/insects5040952","article-title":"Efficacy of commercially available invertebrate predators against <italic>Drosophila suzukii<\/italic>","volume":"5","author":"A Cuthbertson","year":"2014","journal-title":"Insects"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1093\/jee\/tou100","article-title":"Sucrose improves insecticide activity against <italic>Drosophila suzukii<\/italic> (Diptera: Drosophilidae)","volume":"108","author":"RS Cowles","year":"2015","journal-title":"J Econ Entomol"},{"key":"ref33","first-page":"764","article-title":"High throughput sequencing reveals <italic>Drosophila suzukii<\/italic> responses to insecticides","volume":"309","author":"R Mishra","year":"2017","journal-title":"Insect Sci"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"e6063","DOI":"10.1371\/journal.pone.0006063","article-title":"Quantification of food intake in <italic>Drosophila<\/italic>","volume":"4","author":"R Wong","year":"2009","journal-title":"PLoS ONE"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"8253","DOI":"10.1073\/pnas.0702726104","article-title":"Prandiology of <italic>Drosophila<\/italic> and the CAFE assay","volume":"104","author":"WW Ja","year":"2007","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"e223","DOI":"10.1371\/journal.pbio.0030223","article-title":"Calories do not explain extension of life span by dietary restriction in <italic>Drosophila<\/italic>","volume":"3","author":"W Mair","year":"2005","journal-title":"PLoS Biol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1242\/jeb.197.1.215","article-title":"Regulation of feeding behavior in adult <italic>Drosophila melanogaster<\/italic> varies with feeding regime and nutritional state","volume":"197","author":"RS Edgecomb","year":"1994","journal-title":"J Exp Biol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/0022-1910(70)90110-1","article-title":"Quantification of dietary choline utilization in adult <italic>Drosophila melanogaster<\/italic> by radioisotope methods","volume":"16","author":"BW Geer","year":"1970","journal-title":"J Insect Physiol"},{"key":"ref39","first-page":"813","article-title":"Compensatory ingestion upon dietary restriction in <italic>Drosophila melanogaster<\/italic>","volume":"2","author":"GB Carvalho","year":"2005","journal-title":"Nat Chem Biol"},{"key":"ref40","first-page":"535","article-title":"Quantifying <italic>Drosophila<\/italic> food intake: comparative analysis of current methodology","volume":"11","author":"SA Deshpande","year":"2014","journal-title":"Nat Chem Biol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1093\/aesa\/83.3.632","article-title":"Mediterranean fruit fly (Diptera: Tephritidae) in nature: location and diel pattern of feeding and other activities on fruiting and nonfruiting hosts and nonhosts","volume":"83","author":"J Hendrichs","year":"1990","journal-title":"Ann Entomol Soc Am"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1017\/S0007485399000255","article-title":"Impact of adult diet on demographic and population parameters of the tropical fruit fly <italic>Anastrepha serpentina<\/italic> (Diptera: Tephritidae)","volume":"89","author":"I J\u00e1come","year":"1999","journal-title":"Bull Entomol Res"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1079\/BER2006421","article-title":"Contribution of natural food sources to reproductive behaviour, fecundity and longevity of <italic>Ceratitis cosyra<\/italic>, <italic>C<\/italic>. <italic>fasciventris<\/italic> and <italic>C<\/italic>. <italic>capitata<\/italic> (Diptera: Tephritidae)","volume":"96","author":"A Manrakhan","year":"2006","journal-title":"Bull Entomol Res"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/jee\/88.1.1","article-title":"Natural Variation: A complicating factor in bioassays with chemical and microbial pesticides","volume":"88","author":"JL Robertson","year":"1995","journal-title":"J Econ Entomol"},{"key":"ref45","article-title":"Bioassays of entomopatogenic microbes and nematodes","author":"A Navon","year":"2000"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"3547","DOI":"10.1242\/jeb.006924","article-title":"Natural variation in food acquisition mediated via a <italic>Drosophila<\/italic> cGMP-dependent protein kinase","volume":"210","author":"KR Kaun","year":"2007","journal-title":"J Exp Biol"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1002\/ps.2076","article-title":"Insecticide resistance of the oriental fruit fly, <italic>Bactrocera dorsalis<\/italic> (Hendel) (Diptera: Tephritidae), in mainland China","volume":"67","author":"T Jin","year":"2011","journal-title":"Pest Manag Sci"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1016\/j.cub.2006.02.064","article-title":"Allocrine modulation of feeding behavior by the sex peptide of <italic>Drosophila<\/italic>","volume":"16","author":"GB Carvalho","year":"2006","journal-title":"Current Biology"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1006\/jipa.1999.4913","article-title":"Virulence variability of the <italic>Drosophila<\/italic> C virus and effects of the microparasite on demographic parameters of the host (<italic>Drosophila melanogaster)<\/italic>","volume":"75","author":"E Gravot","year":"2000","journal-title":"J Invertebr Pathol"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.jip.2009.02.003","article-title":"The <italic>Drosophila<\/italic> Nora virus is an enteric virus, transmitted via feces","volume":"101","author":"MS Habayeb","year":"2009","journal-title":"J Invertebr Pathol"},{"key":"ref51","first-page":"749","article-title":"Diversity of <italic>Bacillus thuringiensis<\/italic> strains isolated from citrus orchards in Spain and evaluation of their insecticidal activity against <italic>Ceratitis capitata<\/italic>","volume":"19","author":"JC Vidal-Quist","year":"2009","journal-title":"J Microbiol Biotech"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"994","DOI":"10.1128\/AEM.02424-09","article-title":"<italic>Musca domestica<\/italic> salivary gland hypertrophy virus, a globally distributed insect virus that infects and sterilizes female houseflies","volume":"76","author":"P Prompiboon","year":"2010","journal-title":"Appl Environ Microbiol"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1093\/jee\/tow062","article-title":"Biological activity of <italic>Bacillus thuringiensis<\/italic> in <italic>Drosophila suzukii<\/italic> (Diptera: Drosophilidae)","volume":"109","author":"J Cossentine","year":"2016","journal-title":"J Econ Entomol"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0210545","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,11]],"date-time":"2022-09-11T15:32:38Z","timestamp":1662910358000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0210545"}},"subtitle":[],"editor":[{"given":"Nikos T.","family":"Papadopoulos","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2019,2,7]]},"references-count":53,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2019,2,7]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0210545","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,2,7]]}}}