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Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www-ididas.iaea.org\/IDIDAS\/start.htm\" xlink:type=\"simple\">http:\/\/www-ididas.iaea.org\/IDIDAS\/start.htm<\/ext-link>"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1007\/BF00128017","article-title":"Ceratitis capitata\u2014a suitable case for genetic sexing","volume":"58","author":"AS Robinson","year":"1982","journal-title":"Genetica"},{"key":"ref12","first-page":"185","article-title":"Fruit Fly Pests: A World Assessment of Their Biology and Management","author":"G Franz","year":"1996"},{"key":"ref13","first-page":"427","article-title":"Sterile Insect Technique Principles and Practice in Area-Wide Integrated Pest Management","author":"G Franz","year":"2005"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1603\/0022-0493-97.5.1547","article-title":"Medfly (Diptera: Tephritidae) genetic sexing: large-scale field 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Fisher","year":"2000"},{"key":"ref21","first-page":"953","article-title":"Encyclopedia of Insects","author":"J Hendrichs","year":"2009"},{"key":"ref22","first-page":"843","article-title":"Fruit Flies (Tephritidae): Phylogeny and Evolution of Behavior","author":"JP Cayol","year":"2000"},{"key":"ref23","first-page":"379","article-title":"Insects at Low Temperature","author":"RA Leopold","year":"1991"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"e0160232","DOI":"10.1371\/journal.pone.0160232","article-title":"Cryopreservation of embryos of the Mediterranean fruit fly Ceratitis capitata Vienna 8 genetic sexing strain","volume":"11","author":"AA Augustinos","year":"2016","journal-title":"PLoS One"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1603\/0013-8746(2001)094[0695:COEOTN]2.0.CO;2","article-title":"Cryopreservation of embryos of the New World screwworm Cochliomyia hominivorax (Diptera: Calliphoridae)","volume":"94","author":"RA 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Product Quality Control for Sterile Mass-Reared and Released Tephritid Fruit Flies. Austria, Vienna: IAEA; 2014."},{"key":"ref34","doi-asserted-by":"crossref","first-page":"512","DOI":"10.2307\/3494025","article-title":"A cage and support framework for behavioral tests of fruit flies in the field","volume":"66","author":"CO Calkins","year":"1983","journal-title":"Fla Entomol"},{"key":"ref35","author":"RL Ott","year":"2001"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1603\/EC11421","article-title":"Genetic consequences of domestication and mass rearing of pest fruit fly Bactrocera tryoni (Diptera: Tephritidae)","volume":"105","author":"AS Gilchrist","year":"2012","journal-title":"J Econ Entomol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1111\/jen.12042","article-title":"Genetic changes during laboratory domestication of an olive fly SIT strain","volume":"138","author":"NE Zygouridis","year":"2014","journal-title":"J Appl Entomol"},{"key":"ref38","first-page":"1","article-title":"The Mediterranean fruit fly and its bacteria\u2014potential for improving sterile insect technique operations","volume":"135","author":"B Yuval","year":"2010","journal-title":"J Appl Entomol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/S1226-8615(08)60177-6","article-title":"Eradication of the melon fly, Bactrocera cucurbitae, from Okinawa, Japan, by means of the sterile insect technique, with special emphasis on the role of basic studies","volume":"6","author":"Y It\u00f4","year":"2003","journal-title":"J Asia Pac Entomol"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0211259","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,9]],"date-time":"2020-05-09T08:49:38Z","timestamp":1589014178000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0211259"}},"subtitle":[],"editor":[{"given":"David A.","family":"Lightfoot","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2019,1,25]]},"references-count":39,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2019,1,25]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0211259","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,1,25]]}}}