{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T07:56:26Z","timestamp":1791532586459,"version":"4.3.4"},"reference-count":49,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2019,4,29]],"date-time":"2019-04-29T00:00:00Z","timestamp":1556496000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008982","name":"National Science Foundation","doi-asserted-by":"publisher","award":["1616865"],"award-info":[{"award-number":["1616865"]}],"id":[{"id":"10.13039\/501100008982","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008982","name":"National Science Foundation","doi-asserted-by":"publisher","award":["1616845"],"award-info":[{"award-number":["1616845"]}],"id":[{"id":"10.13039\/501100008982","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001298","name":"Presbyterian Health Foundation","doi-asserted-by":"crossref","award":["GRF00001102"],"award-info":[{"award-number":["GRF00001102"]}],"id":[{"id":"10.13039\/100001298","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["P20GM103640"],"award-info":[{"award-number":["P20GM103640"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0211525","type":"journal-article","created":{"date-parts":[[2019,4,29]],"date-time":"2019-04-29T15:19:47Z","timestamp":1556551187000},"page":"e0211525","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":9,"title":["Protein features for assembly of the RNA editing helicase 2 subcomplex (REH2C) in Trypanosome holo-editosomes"],"prefix":"10.1371","volume":"14","author":[{"given":"Vikas","family":"Kumar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pawan K.","family":"Doharey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shelly","family":"Gulati","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua","family":"Meehan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary G.","family":"Martinez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karrisa","family":"Hughes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blaine H. M.","family":"Mooers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0545-1508","authenticated-orcid":true,"given":"Jorge","family":"Cruz-Reyes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2019,4,29]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"e1502","DOI":"10.1002\/wrna.1502","article-title":"Dynamic RNA holoeditosomes with subcomplex variants: Insights into the control of trypanosome editing","author":"J Cruz-Reyes","year":"2018","journal-title":"WIREs RNA"},{"key":"ref2","article-title":"The insect-phase gRNA transcriptome in Trypanosoma brucei","author":"DJ Koslowsky","year":"2013","journal-title":"Nucleic acids research"},{"issue":"4","key":"ref3","doi-asserted-by":"crossref","first-page":"e0123441","DOI":"10.1371\/journal.pone.0123441","article-title":"Native Variants of the MRB1 Complex Exhibit Specialized Functions in Kinetoplastid RNA Editing","volume":"10","author":"BR Madina","year":"2015","journal-title":"PloS one"},{"issue":"11","key":"ref4","doi-asserted-by":"crossref","first-page":"5753","DOI":"10.1074\/jbc.M115.708164","article-title":"REH2C Helicase and GRBC Subcomplexes May Base Pair through mRNA and Small Guide RNA in Kinetoplastid Editosomes","volume":"291","author":"V Kumar","year":"2016","journal-title":"J Biol Chem"},{"issue":"7","key":"ref5","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1261\/rna.044495.114","article-title":"Native mitochondrial RNA-binding complexes in kinetoplastid RNA editing differ in guide RNA composition","volume":"20","author":"BR Madina","year":"2014","journal-title":"RNA"},{"issue":"23","key":"ref6","doi-asserted-by":"crossref","first-page":"4329","DOI":"10.1128\/MCB.01075-14","article-title":"RNA binding and core complexes constitute the U-insertion\/deletion editosome","volume":"34","author":"I Aphasizheva","year":"2014","journal-title":"Mol Cell Biol"},{"key":"ref7","article-title":"Integrity of the core mitochondrial RNA-binding complex 1 is vital for trypanosome RNA editing","author":"Z Huang","year":"2015","journal-title":"RNA"},{"issue":"4","key":"ref8","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1261\/rna.065169.117","article-title":"MRB7260 is essential for productive protein-RNA interactions within the RNA editing substrate binding complex during trypanosome RNA editing","volume":"24","author":"NM McAdams","year":"2018","journal-title":"RNA"},{"issue":"5","key":"ref9","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1261\/rna.055160.115","article-title":"High-throughput sequencing of partially edited trypanosome mRNAs reveals barriers to editing progression and evidence for alternative editing","volume":"22","author":"RM Simpson","year":"2016","journal-title":"RNA"},{"issue":"13","key":"ref10","doi-asserted-by":"crossref","first-page":"7965","DOI":"10.1093\/nar\/gkx458","article-title":"Trypanosome RNA Editing Mediator Complex proteins have distinct functions in gRNA utilization","volume":"45","author":"RM Simpson","year":"2017","journal-title":"Nucleic acids research"},{"issue":"14","key":"ref11","doi-asserted-by":"crossref","first-page":"7096","DOI":"10.1093\/nar\/gkv647","article-title":"Structures of the T. brucei kRNA editing factor MRB1590 reveal unique RNA-binding pore motif contained within an ABC-ATPase fold","volume":"43","author":"PL Shaw","year":"2015","journal-title":"Nucleic acids research"},{"issue":"9","key":"ref12","doi-asserted-by":"crossref","first-page":"4895","DOI":"10.1128\/MCB.17.9.4895","article-title":"Disruption of a gene encoding a novel mitochondrial DEAD-box protein in Trypanosoma brucei affects edited mRNAs","volume":"17","author":"A Missel","year":"1997","journal-title":"Mol Cell Biol"},{"issue":"9","key":"ref13","doi-asserted-by":"crossref","first-page":"3542","DOI":"10.1073\/pnas.1014152108","article-title":"Trypanosome REH1 is an RNA helicase involved with the 3'-5' polarity of multiple gRNA-guided uridine insertion\/deletion RNA editing","volume":"108","author":"F Li","year":"2011","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"2","key":"ref14","first-page":"e1336","article-title":"DEAH-RHA helicase\u2022Znf cofactor systems in kinetoplastid RNA editing and evolutionarily distant RNA processes","volume":"3","author":"J Cruz-Reyes","year":"2016","journal-title":"RNA Dis"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1146\/annurev-biochem-060713-035546","article-title":"RNA helicase proteins as chaperones and remodelers","volume":"83","author":"I Jarmoskaite","year":"2014","journal-title":"Annu Rev Biochem"},{"issue":"13","key":"ref16","doi-asserted-by":"crossref","first-page":"2194","DOI":"10.1038\/emboj.2010.102","article-title":"Prp43p contains a processive helicase structural architecture with a specific regulatory domain","volume":"29","author":"H Walbott","year":"2010","journal-title":"EMBO J"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"931857","DOI":"10.1155\/2015\/931857","article-title":"Regulation of DEAH\/RHA helicases by G-patch proteins","volume":"2015","author":"J Robert-Paganin","year":"2015","journal-title":"Biomed Res Int"},{"issue":"3","key":"ref18","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/j.molcel.2015.10.011","article-title":"Structure of the RNA Helicase MLE Reveals the Molecular Mechanisms for Uridine Specificity and RNA-ATP Coupling","volume":"60","author":"JR Prabu","year":"2015","journal-title":"Mol Cell"},{"issue":"46","key":"ref19","doi-asserted-by":"crossref","first-page":"E3150","DOI":"10.1073\/pnas.1208014109","article-title":"Roles of individual domains in the function of DHX29, an essential factor required for translation of structured mammalian mRNAs","volume":"109","author":"V Dhote","year":"2012","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"6","key":"ref20","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1038\/nprot.2015.053","article-title":"The Phyre2 web portal for protein modeling, prediction and analysis","volume":"10","author":"LA Kelley","year":"2015","journal-title":"Nat Protoc"},{"key":"ref21","article-title":"RNA Metabolism in Mitochondria. Nucleic Acids and Molecular Biology","volume":"34","author":"J. MBHM Cruz-Reyes","year":"2018"},{"issue":"1","key":"ref22","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.tibs.2010.07.008","article-title":"RNA helicases at work: binding and rearranging","volume":"36","author":"E. Jankowsky","year":"2011","journal-title":"Trends in biochemical sciences"},{"issue":"2","key":"ref23","doi-asserted-by":"crossref","first-page":"1220","DOI":"10.1074\/jbc.M109.051862","article-title":"REH2 RNA helicase in kinetoplastid mitochondria: ribonucleoprotein complexes and essential motifs for unwinding and guide RNA (gRNA) binding","volume":"285","author":"A Hernandez","year":"2010","journal-title":"J Biol Chem"},{"issue":"2","key":"ref24","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/j.jmb.2006.08.016","article-title":"Structure of Aart, a designed six-finger zinc finger peptide, bound to DNA","volume":"363","author":"DJ Segal","year":"2006","journal-title":"J Mol Biol"},{"issue":"4","key":"ref25","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1038\/nsmb.2243","article-title":"Structure of N-terminal domain of ZAP indicates how a zinc-finger protein recognizes complex RNA","volume":"19","author":"S Chen","year":"2012","journal-title":"Nat Struct Mol Biol"},{"issue":"1","key":"ref26","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1017\/S0033583510000089","article-title":"The discovery of zinc fingers and their development for practical applications in gene regulation and genome manipulation","volume":"43","author":"A. Klug","year":"2010","journal-title":"Q Rev Biophys"},{"issue":"6","key":"ref27","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1016\/S0092-8674(00)81062-4","article-title":"Direct visualization of uridylate deletion in vitro suggests a mechanism for kinetoplastid RNA editing","volume":"84","author":"SD Seiwert","year":"1996","journal-title":"Cell"},{"issue":"13","key":"ref28","doi-asserted-by":"crossref","first-page":"4069","DOI":"10.1093\/emboj\/16.13.4069","article-title":"Purification of a functional enzymatic editing complex from Trypanosoma brucei mitochondria","volume":"16","author":"LN Rusche","year":"1997","journal-title":"EMBO J"},{"issue":"3","key":"ref29","doi-asserted-by":"crossref","first-page":"950","DOI":"10.1093\/nar\/gkm1108","article-title":"Structure-function analysis of the RNA helicase maleless","volume":"36","author":"A Izzo","year":"2008","journal-title":"Nucleic acids research"},{"issue":"11","key":"ref30","first-page":"1875","article-title":"RNA recognition by double-stranded RNA binding domains: a matter of shape and sequence","volume":"70","author":"G Masliah","year":"2013","journal-title":"Cell Mol Life Sci"},{"issue":"2","key":"ref31","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.1093\/nar\/gkt985","article-title":"Insights into the activation of the helicase Prp43 by biochemical studies and structural mass spectrometry","volume":"42","author":"H Christian","year":"2014","journal-title":"Nucleic acids research"},{"issue":"10","key":"ref32","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1007\/s13238-010-0117-8","article-title":"DEXH-Box protein DHX30 is required for optimal function of the zinc-finger antiviral protein","volume":"1","author":"P Ye","year":"2010","journal-title":"Protein & cell"},{"issue":"23","key":"ref33","doi-asserted-by":"crossref","first-page":"12781","DOI":"10.1128\/JVI.78.23.12781-12787.2004","article-title":"The zinc finger antiviral protein directly binds to specific viral mRNAs through the CCCH zinc finger motifs","volume":"78","author":"X Guo","year":"2004","journal-title":"J Virol"},{"issue":"8","key":"ref34","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1016\/j.bbagrm.2013.04.003","article-title":"RNA helicases involved in U-insertion\/deletion-type RNA editing","volume":"1829","author":"E Kruse","year":"2013","journal-title":"Biochimica et biophysica acta"},{"issue":"22","key":"ref35","doi-asserted-by":"crossref","first-page":"19320","DOI":"10.1074\/jbc.M111.228965","article-title":"Endonuclease associations with three distinct editosomes in Trypanosoma brucei","volume":"286","author":"J Carnes","year":"2011","journal-title":"J Biol Chem"},{"issue":"42","key":"ref36","doi-asserted-by":"crossref","first-page":"E6476","DOI":"10.1073\/pnas.1610177113","article-title":"The Architecture of Trypanosoma brucei editosomes","volume":"113","author":"SM McDermott","year":"2016","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"8","key":"ref37","doi-asserted-by":"crossref","first-page":"4667","DOI":"10.1093\/nar\/gkx116","article-title":"In vivo cleavage specificity of Trypanosoma brucei editosome endonucleases","volume":"45","author":"J Carnes","year":"2017","journal-title":"Nucleic acids research"},{"issue":"5","key":"ref38","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1261\/rna.2446311","article-title":"MRB3010 is a core component of the MRB1 complex that facilitates an early step of the kinetoplastid RNA editing process","volume":"17","author":"ML Ammerman","year":"2011","journal-title":"RNA"},{"issue":"1","key":"ref39","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/S0166-6851(99)00002-X","article-title":"A tightly regulated inducible expression system for conditional gene knock-outs and dominant-negative genetics in Trypanosoma brucei","volume":"99","author":"E Wirtz","year":"1999","journal-title":"Molecular and biochemical parasitology"},{"issue":"1","key":"ref40","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.jmb.2008.05.003","article-title":"Determinants for association and guide RNA-directed endonuclease cleavage by purified RNA editing complexes from Trypanosoma brucei","volume":"381","author":"A Hernandez","year":"2008","journal-title":"J Mol Biol"},{"issue":"4","key":"ref41","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1261\/rna.2194603","article-title":"Identification of novel components of Trypanosoma brucei editosomes","volume":"9","author":"AK Panigrahi","year":"2003","journal-title":"RNA"},{"issue":"12","key":"ref42","doi-asserted-by":"crossref","first-page":"5637","DOI":"10.1093\/nar\/gks211","article-title":"Architecture of the trypanosome RNA editing accessory complex, MRB1","volume":"40","author":"ML Ammerman","year":"2012","journal-title":"Nucleic acids research"},{"issue":"2","key":"ref43","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.molcel.2008.08.023","article-title":"Guide RNA-binding complex from mitochondria of trypanosomatids","volume":"32","author":"J Weng","year":"2008","journal-title":"Mol Cell"},{"issue":"13","key":"ref44","doi-asserted-by":"crossref","first-page":"7233","DOI":"10.1074\/jbc.270.13.7233","article-title":"RNA ligase and its involvement in guide RNA\/mRNA chimera formation. Evidence for a cleavage-ligation mechanism of Trypanosoma brucei mRNA editing","volume":"270","author":"R Sabatini","year":"1995","journal-title":"J Biol Chem"},{"issue":"5","key":"ref45","doi-asserted-by":"crossref","first-page":"970","DOI":"10.1261\/rna.888808","article-title":"TbRGG1, an essential protein involved in kinetoplastid RNA metabolism that is associated with a novel multiprotein complex","volume":"14","author":"H Hashimi","year":"2008","journal-title":"RNA"},{"issue":"6","key":"ref46","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1016\/0092-8674(92)90080-V","article-title":"Kinetoplastid RNA editing: in vitro formation of cytochrome b gRNA-mRNA chimeras from synthetic substrate RNAs","volume":"68","author":"ME Harris","year":"1992","journal-title":"Cell"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0076-6879(07)24002-9","article-title":"Uridine insertion\/deletion editing activities","volume":"424","author":"J Carnes","year":"2007","journal-title":"Methods in enzymology"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/978-1-62703-646-7_6","article-title":"Clustal Omega, accurate alignment of very large numbers of sequences","volume":"1079","author":"F Sievers","year":"2014","journal-title":"Methods Mol Biol"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"D222","DOI":"10.1093\/nar\/gku1221","article-title":"CDD: NCBI's conserved domain database","volume":"43","author":"A Marchler-Bauer","year":"2015","journal-title":"Nucleic acids research"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0211525","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,9]],"date-time":"2020-05-09T03:11:58Z","timestamp":1588993918000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0211525"}},"subtitle":[],"editor":[{"given":"Monika","family":"Oberer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,4,29]]},"references-count":49,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2019,4,29]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0211525","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/524702","asserted-by":"object"}]},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,29]]}}}