{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T14:58:38Z","timestamp":1787669918619,"version":"build-2736575974"},"reference-count":62,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2019,2,26]],"date-time":"2019-02-26T00:00:00Z","timestamp":1551139200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0211985","type":"journal-article","created":{"date-parts":[[2019,2,26]],"date-time":"2019-02-26T18:58:03Z","timestamp":1551207483000},"page":"e0211985","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":54,"title":["Mobile genomic element diversity in world collection of safflower (Carthamus tinctorius L.) panel using iPBS-retrotransposon markers"],"prefix":"10.1371","volume":"14","author":[{"given":"Fawad","family":"Ali","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdurrahim","family":"Y\u0131lmaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Azhar","family":"Nadeem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ephrem","family":"Habyarimana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7237-937X","authenticated-orcid":true,"given":"Ilhan","family":"Suba\u015f\u0131","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Amjad","family":"Nawaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hassan Javed","family":"Chaudhary","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Qasim","family":"Shahid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sezai","family":"Erci\u015fli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Abu Bakar","family":"Zia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8053-689X","authenticated-orcid":true,"given":"Gyuhwa","family":"Chung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7470-0080","authenticated-orcid":true,"given":"Faheem Shehzad","family":"Baloch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2019,2,26]]},"reference":[{"issue":"5","key":"ref1","first-page":"2723","article-title":"Assessment of genetic diversity in safflower (<italic>Carthamus tinct<\/italic>o<italic>rius<\/italic> L.) genotypes through morphological and SSR marker","volume":"6","author":"S Kumari","year":"2017","journal-title":"J Pharmacogn Phytochem"},{"key":"ref2","article-title":"Safflower: Oil seed crops","author":"EA Weiss","year":"2000"},{"issue":"4","key":"ref3","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1007\/s00334-009-0212-z","article-title":"<italic>Carthamus<\/italic> species in the ancient Near East and south-eastern Europe: Archaeobotanical evidence for their distribution and use as a source of oil","volume":"18","author":"E Marinova","year":"2009","journal-title":"Veg Hist Archaeobot"},{"issue":"5","key":"ref4","doi-asserted-by":"crossref","first-page":"831","DOI":"10.3732\/ajb.0900137","article-title":"Population genetic analysis of safflower (<italic>Carthamus tinctorius<\/italic> L.; Asteraceae) reveals a near Eastern origin and five centers of diversity","volume":"97","author":"MA Chapman","year":"2010","journal-title":"Am J Bot"},{"issue":"4","key":"ref5","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1007\/BF02860678","article-title":"Centers of plant diversity and conservation of crop germplasm: Safflower","volume":"23","author":"PF Knowles","year":"1969","journal-title":"Econ Bot"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1007\/BF00132903","article-title":"Evaluation of the germ plasm collection of safflower, <italic>carthamus tinctorius<\/italic> L. V. distribution and regional divergence for morphological characters","volume":"24","author":"A. Ashri","year":"1975","journal-title":"Euphytica"},{"key":"ref7","unstructured":"fao.org\/faostat\/en\/#data\/QD"},{"key":"ref8","unstructured":"Nimbkar N. Issues in safflower production in India. In: Safflower: Unexploited potential and world adaptability. Proceedings of the Seventh International Safflower Conference, Wagga Wagga, New South Wales, Australia. (2008)"},{"key":"ref9","article-title":"Characterization of genetic diversity in Turkish common bean gene pool using phenotypic and whole-genome DArTseq-generated silicoDArT marker information","author":"MA Nadeem","year":"2018","journal-title":"PlosONE"},{"issue":"1\u20132","key":"ref10","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1007\/s10681-005-1681-5","article-title":"An introduction to markers, quantitative trait loci (QTL) mapping and marker-assisted selection for crop improvement: The basic concepts","volume":"142","author":"BCY Collard","year":"2005","journal-title":"Euphytica"},{"issue":"5","key":"ref11","doi-asserted-by":"crossref","first-page":"1299","DOI":"10.1007\/s11105-014-0828-8","article-title":"Assessment of genetic diversity and population structure in a global reference collection of 531 accessions of <italic>Carthamus tinctorius<\/italic> L. (Safflower) using AFLP markers","volume":"33","author":"S Kumar","year":"2015","journal-title":"Plant Mol Biol Rep"},{"issue":"9","key":"ref12","article-title":"Association mapping for important agronomic traits in safflower (<italic>Carthamus tinctorius<\/italic> L.) core collection using microsatellite markers","volume":"402","author":"H Ambreen","year":"2018","journal-title":"Front Plant Sci"},{"issue":"8","key":"ref13","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0135443","article-title":"Development of genomic microsatellite markers in <italic>Carthamus tinctorius<\/italic> L.(Safflower) using next generation sequencing and assessment of their cross-species transferability and utility for diversity analysis","volume":"10","author":"H Ambreen","year":"2015","journal-title":"PLoSONE"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/s10681-007-9556-6","article-title":"Study of genetic diversity in safflower genotypes using agro-morphological traits and RAPD markers","volume":"163","author":"F Amini","year":"2008","journal-title":"Euphytica"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1728","DOI":"10.2135\/cropsci2006.12.0757","article-title":"Characterizing safflower germplasm with AFLP molecular markers","volume":"47","author":"RC Johnson","year":"2007","journal-title":"Crop Sci"},{"issue":"1","key":"ref16","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/s10722-008-9338-6","article-title":"Relationships among different geographical groups, agro-morphology, fatty acid composition and RAPD marker diversity in safflower (<italic>Carthamus tinctorius<\/italic> L.)","volume":"56","author":"MA Khan","year":"2009","journal-title":"Genet Resour Crop Evol"},{"issue":"1","key":"ref17","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1111\/1755-0998.12146","article-title":"Genetic assessment of safflower (<italic>Carthamus tinctorius<\/italic> L.) collection with microsatellite markers acquired via pyrosequencing method","volume":"14","author":"GA Lee","year":"2014","journal-title":"Mol Ecol Resour"},{"issue":"10","key":"ref18","doi-asserted-by":"crossref","first-page":"1640","DOI":"10.3732\/ajb.1400079","article-title":"Genetic diversity in <italic>Carthamus tinctorius<\/italic> (Asteraceae; safflower), an underutilized oilseed crop","volume":"10","author":"SA Pearl","year":"2014","journal-title":"Am J Bot"},{"issue":"3","key":"ref19","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1007\/s10709-008-9292-4","article-title":"Assaying polymorphism at DNA level for genetic diversity diagnostics of the safflower (<italic>Carthamus tinctorius<\/italic> L.) world germplasm resources","volume":"135","author":"D Sehgal","year":"2009","journal-title":"Genetica"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1007\/s10722-006-9192-3","article-title":"Genetic diversity and relationships among safflower (<italic>Carthamus tinctorius<\/italic> L.) analyzed by inter-simple sequence repeats (ISSRs)","volume":"54","author":"YX Yang","year":"2007","journal-title":"Genet Resour Crop Evol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/0168-9525(89)90039-5","article-title":"Eukaryotic transposable elements and genome evolution","volume":"5","author":"DJ Finnegan","year":"1989","journal-title":"Trends Genet"},{"issue":"274","key":"ref22","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1126\/science.274.5288.765","article-title":"Nested retrotransposons in the intergenic regions of the maize genome","volume":"5288","author":"P SanMiguel","year":"1996","journal-title":"Science"},{"issue":"2","key":"ref23","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1080\/13102818.2017.1400401","article-title":"DNA molecular markers in plant breeding: current status and recent advancements in the genomic selection and genome editing","volume":"32","author":"MA Nadeem","year":"2018","journal-title":"Biotechnol Biotechnol Equip"},{"key":"ref24","article-title":"Phylogenetic and taxonomic relationship of turkish wild and cultivated emmer (<italic>triticum turgidum<\/italic> ssp. <italic>dicoccoides<\/italic>) revealed by iPBSretrotransposons markers","author":"M Arystanbekkyzy","year":"2018","journal-title":"Int J Agric Biol"},{"issue":"8","key":"ref25","doi-asserted-by":"crossref","first-page":"1419","DOI":"10.1007\/s00122-010-1398-2","article-title":"iPBS: a universal method for DNA fingerprinting and retrotransposon isolation","volume":"121","author":"R Kalendar","year":"2010","journal-title":"Theor Appl Genet"},{"issue":"4","key":"ref26","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1007\/s13562-012-0175-5","article-title":"IPBS-Retrotransposon-based genetic diversity and relationship among wild annual <italic>Cicer<\/italic> species","volume":"22","author":"EE Andeden","year":"2013","journal-title":"J Plant Biochem Biotechnol"},{"key":"ref27","article-title":"Exploring the genetic diversity and population structure of Turkish common bean germplasm by the iPBS-retrotransposons markers","author":"MF Ayd\u0131n","year":"2018","journal-title":"Legume Res"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.bse.2015.06.017","article-title":"DNA based iPBS-retrotransposon markers for investigating the population structure of pea (<italic>Pisum sativum<\/italic>) germplasm from Turkey","volume":"61","author":"FS Baloch","year":"2015","journal-title":"Biochem Syst Ecol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.bse.2014.12.002","article-title":"Inter-primer binding site retrotransposon and inter-simple sequence repeat diversity among wild Lens species","volume":"58","author":"FS Baloch","year":"2015","journal-title":"Biochem Syst Ecol"},{"issue":"3","key":"ref30","doi-asserted-by":"crossref","first-page":"10588","DOI":"10.4238\/2015.September.8.20","article-title":"Genetic bottlenecks in Turkish okra germplasm and utility of iPBS retrotransposon markers for genetic diversity assessment","volume":"14","author":"M Y\u0131ld\u0131z","year":"2015","journal-title":"Genet Mol Res"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1007\/978-1-4020-6295-7_2","article-title":"Genic molecular markers in plants: development and applications","author":"RK Varshney","year":"2007","journal-title":"Genomics-assisted crop improvement: genomics approaches and platforms"},{"issue":"2","key":"ref32","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1007\/s10681-010-0139-6","article-title":"Transferability of non-genic microsatellite and gene-based sunflower markers to safflower","volume":"175","author":"MJ Garcia-Moreno","year":"2010","journal-title":"Euphytica"},{"issue":"2","key":"ref33","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1111\/j.1439-0523.2010.01826.x","article-title":"Development and characterization of genomic microsatellite markers in safflower (<italic>Carthamus tinctorius<\/italic> L.)","volume":"130","author":"YAS Hamdan","year":"2011","journal-title":"Plant Breed"},{"key":"ref34","first-page":"13","article-title":"Isolation of plant DNA from fresh tissue","volume":"12","author":"JJ Doyle","year":"1990","journal-title":"Focus"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"352","DOI":"10.3906\/tar-1508-27","article-title":"High levels of segregation distortion in the molecular linkage map of bread wheat representing West Asia and North Africa region","volume":"40","author":"FS Baloch","year":"2016","journal-title":"Turk J Agric For"},{"key":"ref36","author":"FC Yeh","year":"2000"},{"key":"ref37","article-title":"R: A Language and Environment for Statistical Computing","year":"2013"},{"issue":"4","key":"ref38","doi-asserted-by":"crossref","first-page":"1933","DOI":"10.1093\/genetics\/144.4.1933","article-title":"Testing differentiation in diploid populations","volume":"144","author":"J Goudet","year":"1996","journal-title":"Genetics"},{"issue":"4","key":"ref39","first-page":"950","article-title":"Estimating hierarchical f statistics","volume":"52","author":"RC Yang","year":"1998","journal-title":"Evolution"},{"key":"ref40","first-page":"223","article-title":"Nouvelles recherches sur la distribution florale","volume":"144","author":"P. Jaccard","year":"1908","journal-title":"Bull Soc Vaud Sci Nat"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"2611","DOI":"10.1111\/j.1365-294X.2005.02553.x","article-title":"Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study","volume":"14","author":"G Evanno","year":"2005","journal-title":"Mol Ecol"},{"issue":"1","key":"ref42","first-page":"127","article-title":"Genomic prediction for yield improvement and safeguarding of genetic diversity in CIMMYT spring wheat (&apos;<italic>Triticum aestivum<\/italic> L.)","volume":"10","author":"E. Habyarimana","year":"2016","journal-title":"Aust J Crop Sci"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1146\/annurev.genet.33.1.479","article-title":"Plant retrotransposons","volume":"33","author":"A Kumar","year":"1999","journal-title":"Annu Rev Genet"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1007\/978-94-011-4898-6_10","article-title":"Quasispecies in retrotransposons: a role for sequence variability in Tnt1 evolution","author":"JM Casacuberta","year":"1997","journal-title":"Evolution and Impact of Transposable Elements"},{"issue":"10","key":"ref45","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1038\/nrg2640","article-title":"The impact of retrotransposons on human genome evolution","volume":"10","author":"R Cordaux","year":"2009","journal-title":"Nat Rev Genet"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.bse.2013.10.008","article-title":"Molecular diversity analysis of grape varieties based on iPBS markers","volume":"52","author":"DL Guo","year":"2014","journal-title":"Biochem Syst Ecol"},{"issue":"1","key":"ref47","first-page":"1","article-title":"Molecular diversity analysis of <italic>Tetradium ruticarpum<\/italic> (WuZhuYu) in China based on inter-primer binding site (iPBS) markers and inter-simple sequence repeat (ISSR) markers","volume":"16","author":"XU Jing-Yuan","year":"2018","journal-title":"Chin J Nat Med"},{"issue":"1","key":"ref48","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1139\/g93-024","article-title":"Optimizing parental selection for genetic linkage maps","volume":"36","author":"JA Anderson","year":"1993","journal-title":"Genome"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.bse.2012.04.013","article-title":"Genetic diversity revealed by EST-SSR markers in cultivated and wild safflower","volume":"44","author":"M Barati","year":"2012","journal-title":"Biochem Syst Ecol"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.bse.2014.01.003","article-title":"Discrimination and genetic diversity of cultivated and wild safflowers (<italic>Carthamus<\/italic> spp.) using EST-microsatellites markers","volume":"54","author":"E Derakhshan","year":"2014","journal-title":"Biochem Syst Ecol"},{"issue":"6","key":"ref51","first-page":"881","article-title":"Genetic diversity analysis of safflower (<italic>Carthamus tinctorius<\/italic>) accessions from different geographic origins using ISSR markers","volume":"18","author":"K Houmanat","year":"2016","journal-title":"Int J Agric Biol"},{"issue":"2","key":"ref52","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1007\/s12298-012-0155-1","article-title":"Genetic characterization of Iranian safflower (<italic>Carthamus tinctorius<\/italic> L.) using Inter Simple Sequence Repeats (ISSR) markers","volume":"19","author":"B Panahi","year":"2013","journal-title":"Physiol Mol Bio Plants"},{"issue":"12","key":"ref53","doi-asserted-by":"crossref","first-page":"1764","DOI":"10.5352\/JLS.2010.20.12.1764","article-title":"Phylogenetic Relationships and Genetic Diversity in Collected Resources of <italic>Carthamus tinctorius<\/italic> by Random Amplified Polymorphic DNA Markers","volume":"20","author":"JS Sung","year":"2010","journal-title":"J Life Sci"},{"key":"ref54","article-title":"Assessment of genetic diversity and population structure in a global reference collection of 531 accessions of <italic>Carthamus tinctorius<\/italic> (safflower) using AFLP markers","author":"S Kumar","year":"2014","journal-title":"Plant Mol Biol Rep"},{"key":"ref55","first-page":"1","article-title":"Assessment of genetic diversity and population genetic structure of <italic>Carthamus<\/italic> species and Iranian cultivar collection using developed SSR markers","author":"N Mokhtari","year":"2018","journal-title":"J Genet"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1111\/j.1365-294X.1994.tb00109.x","article-title":"Analysis of population genetic structure with RAPD markers","volume":"3","author":"M Lynch","year":"1994","journal-title":"Mol Ecol"},{"issue":"3","key":"ref57","first-page":"434","article-title":"Clustering analysis of Ethiopian safflower (<italic>Carthamus tinctorius<\/italic>) using ISSR Markers","volume":"5","author":"B Wodajo","year":"2015","journal-title":"Int J Sci Res Publ"},{"issue":"3","key":"ref58","doi-asserted-by":"crossref","first-page":"e33470","DOI":"10.1371\/journal.pone.0033470","article-title":"Genetic structure, linkage disequilibrium and signature of selection in sorghum: lessons from physically anchored DArT markers","volume":"7","author":"S Bouchet","year":"2012","journal-title":"PLoS ONE"},{"key":"ref59","doi-asserted-by":"crossref","first-page":"1898","DOI":"10.1214\/13-AOAS671","article-title":"An algorithm for deciding the number of clusters and validation using simulated data with application to exploring crop population structure","volume":"7","author":"MA Newell","year":"2013","journal-title":"Ann App Stat"},{"issue":"4","key":"ref60","doi-asserted-by":"crossref","first-page":"2664","DOI":"10.3390\/ijms12042664","article-title":"Genetic variation in safflower (<italic>Carthamus tinctorious<\/italic> L.) for seed quality-related traits and inter-simple sequence repeat (ISSR) markers","volume":"12","author":"P Golkar","year":"2011","journal-title":"Int J Mol Sci"},{"issue":"4","key":"ref61","first-page":"6","article-title":"Studies on genetic diversity and classification of safflower (<italic>Carthamus tinctorius<\/italic> L.) germplasm by isozyme techniques","volume":"1","author":"ZW Zhang","year":"2000","journal-title":"J Plant Gene Res"},{"key":"ref62","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1186\/1471-2229-7-60","article-title":"DNA sequence diversity and the origin of cultivated safflower (<italic>Carthamus tinctorius<\/italic> L.; Asteraceae)","volume":"7","author":"MA Chapman","year":"2007","journal-title":"BMC Plant Biol"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0211985","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T17:36:25Z","timestamp":1663004185000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0211985"}},"subtitle":[],"editor":[{"given":"Allah","family":"Bakhsh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,2,26]]},"references-count":62,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2019,2,26]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0211985","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,2,26]]}}}