{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T03:45:20Z","timestamp":1792813520254,"version":"4.5.0"},"reference-count":27,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2019,3,13]],"date-time":"2019-03-13T00:00:00Z","timestamp":1552435200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"crossref","award":["Creation of Innovation Centers for Advanced Interdisciplinary Research Areas Program"],"award-info":[{"award-number":["Creation of Innovation Centers for Advanced Interdisciplinary Research Areas Program"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0212369","type":"journal-article","created":{"date-parts":[[2019,3,13]],"date-time":"2019-03-13T17:25:47Z","timestamp":1552497947000},"page":"e0212369","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":39,"title":["Fabricating retinal pigment epithelial cell sheets derived from human induced pluripotent stem cells in an automated closed culture system for regenerative medicine"],"prefix":"10.1371","volume":"14","author":[{"given":"Erino","family":"Matsumoto","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoshi","family":"Koide","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroko","family":"Hanzawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaharu","family":"Kiyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari","family":"Ohta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junichi","family":"Kuwabara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6616-8402","authenticated-orcid":true,"given":"Shizu","family":"Takeda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masayo","family":"Takahashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2019,3,13]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"14452","DOI":"10.1073\/pnas.1508520112","article-title":"Regenerative medicine: current therapies and future directions","volume":"112","author":"AS Mao","year":"2015","journal-title":"PNAS"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1474","DOI":"10.1056\/NEJMra062326","article-title":"Age-related macular degeneration","volume":"355","author":"PTVM de Jong","year":"2006","journal-title":"N Engl J Med"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"102","DOI":"10.3928\/1542-8877-19910201-12","article-title":"A technique for retinal pigment epithelium transplantation for age-related macular degeneration secondary to extensive subfoveal scarring","volume":"22","author":"GA Peyman","year":"1991","journal-title":"Ophthalmic Surg Lasers Imaging Retina"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1177\/112067219900900310","article-title":"Long-term outcome of RPE allografts in non-immunosuppressed patients with AMD","volume":"9","author":"PV Algvere","year":"1999","journal-title":"Eur J Ophthalmol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"4151","DOI":"10.1167\/iovs.04-0118","article-title":"Outcome of transplantation of autologous retinal pigment epithelium in age-related macular degeneration: a prospective trial","volume":"45","author":"S Binder","year":"2004","journal-title":"Invest Ophthalmol Visual Sci"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1136\/bjo.88.1.110","article-title":"Autologous peripheral retinal pigment epithelium translocation in patients with subfoveal neovascular membranes","volume":"88","author":"JC van Meurs","year":"2004","journal-title":"Br J Ophthalmol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"8309","DOI":"10.1167\/iovs.14-14694","article-title":"Objective evaluation of the degree of pigmentation in human induced pluripotent stem cell-derived RPE","volume":"55","author":"H Kamao","year":"2014","journal-title":"Invest Ophthalmol Visual Sci"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.stemcr.2013.12.007","article-title":"Characterization of human induced pluripotent stem cell-derived retinal pigment epithelium cell sheets aiming for clinical application","volume":"2","author":"H Kamao","year":"2014","journal-title":"Stem Cell Rep"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1038","DOI":"10.1056\/NEJMoa1608368","article-title":"Autologous induced stem-cell-derived retinal cells for macular degeneration","volume":"376","author":"M Mandai","year":"2017","journal-title":"N Engl J Med"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.procbio.2016.09.032","article-title":"Progress and challenges in large-scale expansion of human pluripotent stem cells","volume":"59","author":"C Kropp","year":"2017","journal-title":"Process Biochem"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1177\/2472630317712220","article-title":"Automated cell culture systems and their applications to human pluripotent stem cell studies","volume":"23","author":"M Daniszewski","year":"2017","journal-title":"SLAS Technol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1089\/ten.tea.2009.0454","article-title":"Scalable stirred-suspension bioreactor culture of human pluripotent stem cells","volume":"16","author":"DE Kehoe","year":"2010","journal-title":"Tissue Eng: Part A"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1186\/1472-6750-13-102","article-title":"Large-scale cell production of stem cells for clinical application using the automated cell processing machine","volume":"13","author":"D Kami","year":"2013","journal-title":"BMC Biotechnol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.jbiotec.2013.12.009","article-title":"Investigating the feasibility of scale up and automation of human induced pluripotent stem cells cultured in aggregates in feeder free conditions","volume":"173","author":"FAC Soares","year":"2014","journal-title":"J Biotechnol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"16647","DOI":"10.1038\/srep16647","article-title":"Long-term maintenance of human induced pluripotent stem cells by automated cell culture system","volume":"5","author":"S Konagaya","year":"2015","journal-title":"Sci Rep"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"9010","DOI":"10.1016\/j.biomaterials.2013.07.065","article-title":"Corneal regeneration by transplantation of corneal epithelial cell sheets fabricated with automated cell culture system in rabbit model","volume":"34","author":"T Kobayashi","year":"2013","journal-title":"Biomaterials"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1002\/term.1639","article-title":"A novel closed cell culture device for fabrication of corneal epithelial cell sheets","volume":"9","author":"R Nakajima","year":"2015","journal-title":"J Tissue Eng Regen Med"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1089\/gen.34.09.15","article-title":"Closed processing for cell therapies: engineering risk reduction and patient safety during manufacturing","volume":"34","author":"B Hampson","year":"2014","journal-title":"Genet Eng Biotechnol News"},{"key":"ref19","first-page":"693","article-title":"Wider adoption of regenerative medicine driven by open innovation","volume":"64","author":"K Shu","year":"2016","journal-title":"Hitachi Rev"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1083\/jcb.103.3.755","article-title":"Identification of ZO-I: a high molecular weight polypeptide associated with the tight junction (zonula occludens) in a variety of epithelia","volume":"103","author":"BR Stevenson","year":"1986","journal-title":"J Cell Biol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1152\/physrev.00021.2004","article-title":"The retinal pigment epithelium in visual function","volume":"85","author":"O Strauss","year":"2005","journal-title":"Physiol Rev"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/S0002-9440(10)65138-3","article-title":"Polarized vascular endothelial growth factor secretion by human retinal pigment epithelium and localization of vascular endothelial growth factor receptors on the inner choriocapillaris","volume":"155","author":"HGT Blaauwgeers","year":"1999","journal-title":"Am J Pathol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1392","DOI":"10.1167\/iovs.10-5984","article-title":"Claudin-19 and the barrier properties of the human retinal pigment epithelium","volume":"52","author":"S Peng","year":"2011","journal-title":"Invest Ophthalmol Visual Sci"},{"key":"ref24","first-page":"558","article-title":"Toward the defined and xeno-free differentiation of functional human pluripotent stem cell-derived retinal pigment epithelial cells","volume":"17","author":"H Vaajasaari","year":"2011","journal-title":"Mol Vision"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"C1497","DOI":"10.1152\/ajpcell.00355.2002","article-title":"Na,K-ATPase inhibition alters tight junction structure and permeability in human retinal pigment epithelial cells","volume":"284","author":"SA Rajasekaran","year":"2003","journal-title":"Am J Physiol Cell Physiol"},{"key":"ref26","first-page":"33792","article-title":"Mislocalisation of BEST1 in iPSC-derived retinal pigment epithelial cells from a family with autosomal dominant vitreoretinochoroidopathy (ADVIRC)","volume":"6","author":"A David","journal-title":"Sci Rep"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1177\/2041731416650838","article-title":"Methods for culturing retinal pigment epithelial cells: a review of current protocols and future recommendations","volume":"7","author":"AH Fronk","year":"2016","journal-title":"J Tissue Eng"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0212369","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,13]],"date-time":"2022-09-13T19:38:37Z","timestamp":1663097917000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0212369"}},"subtitle":[],"editor":[{"given":"Majlinda","family":"Lako","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,3,13]]},"references-count":27,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2019,3,13]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0212369","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,13]]}}}