{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T05:59:01Z","timestamp":1780984741615,"version":"3.54.1"},"reference-count":67,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2019,3,11]],"date-time":"2019-03-11T00:00:00Z","timestamp":1552262400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007225","name":"Ministry of Science and Technology","doi-asserted-by":"publisher","award":["MOST 99-2320-B-002-017-MY3, 100-2325-B-002-039, 101-2325-B-002-036-, 103-2628-B-002-006-MY3-, and 106-2320-B-002 -038 -MY2"],"award-info":[{"award-number":["MOST 99-2320-B-002-017-MY3, 100-2325-B-002-039, 101-2325-B-002-036-, 103-2628-B-002-006-MY3-, and 106-2320-B-002 -038 -MY2"]}],"id":[{"id":"10.13039\/100007225","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Health Research Institutes grant","award":["NHRI-EX99-9510NC"],"award-info":[{"award-number":["NHRI-EX99-9510NC"]}]},{"name":"Department of Medical Research in National Taiwan University Hospital and the Aim for the Top University Program of National Taiwan University","award":["grant number 33474"],"award-info":[{"award-number":["grant number 33474"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0213594","type":"journal-article","created":{"date-parts":[[2019,3,11]],"date-time":"2019-03-11T13:35:05Z","timestamp":1552311305000},"page":"e0213594","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":21,"title":["Development of CRISPR\/Cas9-mediated gene disruption systems in Giardia lamblia"],"prefix":"10.1371","volume":"14","author":[{"given":"Zi-Qi","family":"Lin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8420-0303","authenticated-orcid":true,"given":"Soo-Wah","family":"Gan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Szu-Yu","family":"Tung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun-Che","family":"Ho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Hsin","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8604-8085","authenticated-orcid":true,"given":"Chin-Hung","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2019,3,11]]},"reference":[{"issue":"3","key":"ref1","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1128\/CMR.14.3.447-475.2001","article-title":"Biology of Giardia lamblia","volume":"14","author":"RD Adam","year":"2001","journal-title":"Clin Microbiol Rev"},{"issue":"11\u201312","key":"ref2","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1016\/j.ijpara.2005.07.009","article-title":"The zoonotic transmission of Giardia and Cryptosporidium","volume":"35","author":"P.R Hunter","year":"2005","journal-title":"Int J Parasitol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1186\/1471-230X-9-27","article-title":"Development of functional gastrointestinal disorders after Giardia lamblia infection","volume":"9","author":"K Hanevik","year":"2009","journal-title":"BMC Gastroenterol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1208","DOI":"10.3389\/fmicb.2015.01208","article-title":"Drug Development Against the Major Diarrhea-Causing Parasites of the Small Intestine, Cryptosporidium and Giardia","volume":"6","author":"Y Miyamoto","year":"2015","journal-title":"Front Microbiol"},{"issue":"9036","key":"ref5","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1016\/S0140-6736(02)07744-9","article-title":"Effects of stunting, diarrhoeal disease, and parasitic infection during infancy on cognition in late childhood: a follow-up study","volume":"359","author":"DS Berkman","year":"2002","journal-title":"Lancet"},{"issue":"5","key":"ref6","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1111\/j.1442-200x.2005.02110.x","article-title":"Effects of giardiasis on school success, weight and height indices of primary school children in Turkey","volume":"47","author":"A Celiksoz","year":"2005","journal-title":"Pediatr Int"},{"issue":"6","key":"ref7","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1038\/nrmicro2317","article-title":"Behind the smile: cell biology and disease mechanisms of Giardia species","volume":"8","author":"J Ankarklev","year":"2010","journal-title":"Nat Rev Microbiol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.mib.2016.07.019","article-title":"An up-date on Giardia and giardiasis","volume":"34","author":"E Einarsson","year":"2016","journal-title":"Curr Opin Microbiol"},{"issue":"49","key":"ref9","doi-asserted-by":"crossref","first-page":"29307","DOI":"10.1074\/jbc.270.49.29307","article-title":"Identification of a novel Giardia lamblia cyst wall protein with leucine-rich repeats. Implications for secretory granule formation and protein assembly into the cyst wall","volume":"270","author":"HD Luj\u00e1n","year":"1995","journal-title":"J Biol Chem"},{"issue":"5","key":"ref10","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1111\/j.1365-2958.1995.tb02364.x","article-title":"Developmentally regulated expression of a Giardia lamblia cyst wall protein gene","volume":"15","author":"MR Mowatt","year":"1995","journal-title":"Mol Microb"},{"issue":"24","key":"ref11","doi-asserted-by":"crossref","first-page":"21701","DOI":"10.1074\/jbc.M302023200","article-title":"Mining the Giardia lamblia genome for new cyst wall proteins","volume":"278","author":"CH Sun","year":"2003","journal-title":"J Biol Chem"},{"issue":"6","key":"ref12","doi-asserted-by":"crossref","first-page":"3733","DOI":"10.1074\/jbc.M111.298893","article-title":"Regulation of a Myb transcription factor by cyclin dependent kinase 2 in Giardia lamblia","volume":"287","author":"CC Cho","year":"2012","journal-title":"J Biol Chem"},{"issue":"45","key":"ref13","doi-asserted-by":"crossref","first-page":"31021","DOI":"10.1074\/jbc.M805023200","article-title":"Regulation of Cyst Wall Protein Promoters by Myb2 in Giardia lamblia","volume":"283","author":"YC Huang","year":"2008","journal-title":"J Biol Chem"},{"issue":"27","key":"ref14","doi-asserted-by":"crossref","first-page":"17975","DOI":"10.1074\/jbc.M109.012047","article-title":"A novel WRKY-like protein involved in transcriptional activation of cyst wall protein genes in Giardia lamblia","volume":"284","author":"YJ Pan","year":"2009","journal-title":"J Biol Chem"},{"issue":"42","key":"ref15","doi-asserted-by":"crossref","first-page":"32213","DOI":"10.1074\/jbc.M110.156620","article-title":"A novel pax-like protein involved in transcriptional activation of cyst wall protein genes in Giardia lamblia","volume":"285","author":"YT Wang","year":"2010","journal-title":"J Biol Chem"},{"issue":"39","key":"ref16","doi-asserted-by":"crossref","first-page":"34101","DOI":"10.1074\/jbc.M111.280206","article-title":"A Novel E2F-like Protein Involved in Transcriptional Activation of Cyst Wall Protein Genes in Giardia lamblia","volume":"286","author":"LH Su","year":"2011","journal-title":"J Biol Chem"},{"issue":"Pt A","key":"ref17","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.biocel.2016.10.010","article-title":"Specific histone modifications play critical roles in the control of encystation and antigenic variation in the early-branching eukaryote Giardia lamblia","volume":"81","author":"PG Carranza","year":"2016","journal-title":"Int J Biochem Cell Biol"},{"issue":"1","key":"ref18","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1111\/j.1365-2958.2010.07062.x","article-title":"Epigenetic mechanisms regulate stage differentiation in the minimized protozoan Giardia lamblia","volume":"76","author":"S Sonda","year":"2010","journal-title":"Mol Microbiol"},{"issue":"1","key":"ref19","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/S0166-6851(97)00239-9","article-title":"Stable DNA transfection of the primitive protozoan pathogen Giardia lamblia","volume":"92","author":"CH Sun","year":"1998","journal-title":"Mol Biochem Parasitol"},{"issue":"1","key":"ref20","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/S0166-6851(97)00225-9","article-title":"Episomal and integrated maintenance of foreign DNA in Giardia lamblia","volume":"92","author":"SM Singer","year":"1998","journal-title":"Mol Biochem Parasitol"},{"issue":"8","key":"ref21","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/j.ijpara.2014.03.008","article-title":"The Cre\/loxP system in Giardia lamblia: genetic manipulations in a binucleate tetraploid protozoan","volume":"44","author":"PB Wampfler","year":"2014","journal-title":"Int J Parasitol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"13859","DOI":"10.1038\/ncomms13859","article-title":"Cyst-Wall-Protein-1 is fundamental for Golgi-like organelle neogenesis and cyst-wall biosynthesis in Giardia lamblia","volume":"7","author":"JA Ebneter","year":"2016","journal-title":"Nat Commun"},{"issue":"1","key":"ref23","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1046\/j.1462-5822.2001.00094.x","article-title":"Genome ploidy in different stages of the Giardia lamblia life cycle","volume":"3","author":"R Bernander","year":"2001","journal-title":"Cell Microbiol"},{"issue":"2","key":"ref24","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1046\/j.1365-2958.2000.01863.x","article-title":"Inhibition of pyruvate-ferredoxin oxidoreductase gene expression in Giardia lamblia by a virus-mediated hammerhead ribozyme","volume":"36","author":"M Dan","year":"2000","journal-title":"Mol Microbiol"},{"issue":"7","key":"ref25","doi-asserted-by":"crossref","first-page":"3053","DOI":"10.1091\/mbc.e03-10-0744","article-title":"Adaptor protein complex 1 mediates the transport of lysosomal proteins from a Golgi-like organelle to peripheral vacuoles in the primitive eukaryote Giardia lamblia","volume":"15","author":"MC Touz","year":"2004","journal-title":"Mol Biol Cell"},{"issue":"6","key":"ref26","doi-asserted-by":"crossref","first-page":"916","DOI":"10.1128\/EC.00041-09","article-title":"Using morpholinos for gene knockdown in Giardia intestinalis","volume":"8","author":"ML Carpenter","year":"2009","journal-title":"Eukaryot Cell"},{"key":"ref27","article-title":"Robust and stable transcriptional repression in Giardia using CRISPRi","author":"SG McInally","year":"2018","journal-title":"Mol Biol Cell"},{"issue":"8","key":"ref28","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1038\/nbt.2925","article-title":"Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system","volume":"32","author":"M Ghorbal","year":"2014","journal-title":"Nat Biotechnol"},{"issue":"3","key":"ref29","doi-asserted-by":"crossref","first-page":"e01114","DOI":"10.1128\/mBio.01114-14","article-title":"Efficient gene disruption in diverse strains of Toxoplasma gondii using CRISPR\/CAS9","volume":"5","author":"B Shen","year":"2014","journal-title":"MBio"},{"issue":"1","key":"ref30","doi-asserted-by":"crossref","first-page":"e02097","DOI":"10.1128\/mBio.02097-14","article-title":"CRISPR-Cas9-mediated single-gene and gene family disruption in Trypanosoma cruzi","volume":"6","author":"D Peng","year":"2014","journal-title":"MBio"},{"issue":"10","key":"ref31","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1111\/cmi.12456","article-title":"First efficient CRISPR-Cas9-mediated genome editing in Leishmania parasites","volume":"17","author":"L Sollelis","year":"2015","journal-title":"Cell Microbiol"},{"issue":"1","key":"ref32","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1038\/s41598-017-18442-3","article-title":"CRISPR\/Cas9-mediated gene modification and gene knock out in the human-infective parasite Trichomonas vaginalis","volume":"8","author":"BD Janssen","year":"2018","journal-title":"Sci Rep"},{"issue":"10","key":"ref33","doi-asserted-by":"crossref","first-page":"6091","DOI":"10.1093\/nar\/gku241","article-title":"Classification and evolution of type II CRISPR-Cas systems","volume":"42","author":"K Chylinski","year":"2014","journal-title":"Nucleic Acids Res"},{"issue":"6096","key":"ref34","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1126\/science.1225829","article-title":"A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity","volume":"337","author":"M Jinek","year":"2012","journal-title":"Science"},{"issue":"6121","key":"ref35","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1126\/science.1232033","article-title":"RNA-guided human genome engineering via Cas9","volume":"339","author":"P Mali","year":"2013","journal-title":"Science"},{"issue":"4","key":"ref36","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/j.molcel.2012.07.029","article-title":"Playing the end game: DNA double-strand break repair pathway choice","volume":"47","author":"JR Chapman","year":"2012","journal-title":"Mol Cell"},{"issue":"1","key":"ref37","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.biotechadv.2014.12.006","article-title":"The CRISPR\/Cas9 system for plant genome editing and beyond","volume":"33","author":"L Bortesi","year":"2015","journal-title":"Biotechnol Adv"},{"issue":"9","key":"ref38","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1002\/em.20481","article-title":"Dependence of DNA double strand break repair pathways on cell cycle phase in human lymphoblastoid cells","volume":"50","author":"Y Takashima","year":"2009","journal-title":"Environ Mol Mutagen"},{"issue":"5","key":"ref39","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1038\/nbt.3198","article-title":"Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells","volume":"33","author":"VT Chu","year":"2015","journal-title":"Nat Biotechnol"},{"issue":"5","key":"ref40","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1038\/nbt.3190","article-title":"Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining","volume":"33","author":"T Maruyama","year":"2015","journal-title":"Nat Biotechnol"},{"issue":"21","key":"ref41","doi-asserted-by":"crossref","first-page":"3959","DOI":"10.1111\/febs.14661","article-title":"Autocyclized and oxidized forms of SCR7 induce cancer cell death by inhibiting nonhomologous DNA end joining in a Ligase IV dependent manner","volume":"285","author":"SV Vartak","year":"2018","journal-title":"FEBS J"},{"issue":"29","key":"ref42","doi-asserted-by":"crossref","first-page":"5105","DOI":"10.1038\/sj.onc.1207661","article-title":"Myeloid Leukemia Factor 1 inhibits erythropoietin-induced differentiation, cell cycle exit and p27Kip1 accumulation","volume":"23","author":"LN Winteringham","year":"2004","journal-title":"Oncogene"},{"issue":"13","key":"ref43","doi-asserted-by":"crossref","first-page":"4986","DOI":"10.1073\/pnas.1117317109","article-title":"Myeloid leukemia factor is a conserved regulator of RUNX transcription factor activity involved in hematopoiesis","volume":"109","author":"S Bras","year":"2012","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"4","key":"ref44","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1016\/S1534-5807(02)00297-6","article-title":"A genomic switch at the transition from cell proliferation to terminal differentiation in the Drosophila eye","volume":"3","author":"H Jasper","year":"2002","journal-title":"Dev Cell"},{"issue":"20","key":"ref45","doi-asserted-by":"crossref","first-page":"5559","DOI":"10.1093\/emboj\/18.20.5559","article-title":"HLS7, a hemopoietic lineage switch gene homologous to the leukemia-inducing gene MLF1","volume":"18","author":"JH Williams","year":"1999","journal-title":"EMBO J"},{"issue":"7","key":"ref46","doi-asserted-by":"crossref","first-page":"2196","DOI":"10.1039\/c0mb00349b","article-title":"Identification of a high-affinity network of secretagogin-binding proteins involved in vesicle secretion","volume":"7","author":"MC Bauer","year":"2011","journal-title":"Mol Biosyst"},{"issue":"2","key":"ref47","first-page":"265","article-title":"The t(3;5)(q25.1;q34) of myelodysplastic syndrome and acute myeloid leukemia produces a novel fusion gene, NPM-MLF1","volume":"12","author":"N Yoneda-Kato","year":"1996","journal-title":"Oncogene"},{"issue":"2","key":"ref48","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.molbiopara.2007.07.015","article-title":"Neomycin and puromycin affect gene expression in Giardia lamblia stable transfection","volume":"156","author":"LH Su","year":"2007","journal-title":"Mol Biochem Parasitol"},{"issue":"4","key":"ref49","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/0035-9203(83)90120-7","article-title":"Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile","volume":"77","author":"DB Keister","year":"1983","journal-title":"Trans R Soc Trop Med Hyg"},{"issue":"5","key":"ref50","doi-asserted-by":"crossref","first-page":"e2218","DOI":"10.1371\/journal.pntd.0002218","article-title":"DNA topoisomerase II is involved in regulation of cyst wall protein genes and differentiation in Giardia lamblia","volume":"7","author":"BC Lin","year":"2013","journal-title":"PLoS Negl Trop Dis"},{"key":"ref51","article-title":"Molecular Cloning: A Laboratory Manual","author":"J Sambrook","year":"2001"},{"issue":"11","key":"ref52","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1016\/j.ijpara.2008.02.006","article-title":"Incomplete nonsense-mediated mRNA decay in Giardia lamblia","volume":"38","author":"YH Chen","year":"2008","journal-title":"Int J Parasitol"},{"issue":"2","key":"ref53","doi-asserted-by":"crossref","first-page":"e30614","DOI":"10.1371\/journal.pone.0030614","article-title":"Functional redundancy of two Pax-like proteins in transcriptional activation of cyst wall protein genes in Giardia lamblia","volume":"7","author":"SF Chuang","year":"2012","journal-title":"PLoS One"},{"issue":"2","key":"ref54","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1046\/j.1365-2958.1999.01602.x","article-title":"Developmental gene regulation in Giardia lamblia: first evidence for an encystation-specific promoter and differential 5' mRNA processing","volume":"34","author":"LA Knodler","year":"1999","journal-title":"Mol Microbiol"},{"issue":"12","key":"ref55","doi-asserted-by":"crossref","first-page":"e1442","DOI":"10.1371\/journal.pntd.0001442","article-title":"Novel structural components of the ventral disc and lateral crest in Giardia intestinalis","volume":"5","author":"KD Hagen","year":"2011","journal-title":"PLoS Negl Trop Dis"},{"issue":"12","key":"ref56","doi-asserted-by":"crossref","first-page":"5615","DOI":"10.1073\/pnas.92.12.5615","article-title":"Transient transfection and expression of firefly luciferase in Giardia lamblia","volume":"92","author":"J Yee","year":"1995","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"28","key":"ref57","doi-asserted-by":"crossref","first-page":"19699","DOI":"10.1074\/jbc.274.28.19699","article-title":"Identification and characterization of a ran gene promoter in the protozoan pathogen Giardia lamblia","volume":"274","author":"CH Sun","year":"1999","journal-title":"J Biol Chem"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.dnarep.2016.04.004","article-title":"SCR7 is neither a selective nor a potent inhibitor of human DNA ligase IV","volume":"43","author":"GE Greco","year":"2016","journal-title":"DNA Repair (Amst)"},{"issue":"12","key":"ref59","doi-asserted-by":"crossref","first-page":"6630","DOI":"10.1073\/pnas.110152897","article-title":"The nonhomologous end-joining pathway of DNA repair is required for genomic stability and the suppression of translocations","volume":"97","author":"DO Ferguson","year":"2000","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"1","key":"ref60","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.molbiopara.2005.03.003","article-title":"Influence of 5' sequences on expression of the Tet repressor in Giardia lamblia","volume":"142","author":"CH Sun","year":"2005","journal-title":"Mol Biochem Parasitol"},{"issue":"6","key":"ref61","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1016\/j.cell.2016.08.019","article-title":"A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes","volume":"166","author":"SM Sidik","year":"2016","journal-title":"Cell"},{"key":"ref62","doi-asserted-by":"crossref","first-page":"e264","DOI":"10.1038\/mtna.2015.37","article-title":"Off-target Effects in CRISPR\/Cas9-mediated Genome Engineering","volume":"4","author":"XH Zhang","year":"2015","journal-title":"Mol Ther Nucleic Acids"},{"issue":"1","key":"ref63","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1128\/EC.00190-10","article-title":"Rapid tagging and integration of genes in Giardia intestinalis","volume":"10","author":"S Gourguechon","year":"2011","journal-title":"Eukaryot Cell"},{"key":"ref64","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1111\/j.1365-2443.2006.01023.x","article-title":"Characterization of the Drosophila myeloid leukemia factor","volume":"11","author":"S Martin-Lanner\u00e9e","year":"2006","journal-title":"Genes Cells"},{"issue":"1","key":"ref65","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/S0166-6851(99)00163-2","article-title":"Development of a tetracycline controlled gene expression system in the parasitic protozoan Giardia lamblia","volume":"105","author":"CH Sun","year":"2000","journal-title":"Mol Biochem Parasitol"},{"key":"ref66","doi-asserted-by":"crossref","first-page":"e17285","DOI":"10.1371\/journal.pone.0017285","article-title":"The minimal proteome in the reduced mitochondrion of the parasitic protist Giardia intestinalis","volume":"6","author":"PL Jedelsk\u00fd","year":"2011","journal-title":"PLoS One"},{"issue":"16","key":"ref67","doi-asserted-by":"crossref","first-page":"2864","DOI":"10.1128\/MCB.00448-15","article-title":"Probing the Biology of Giardia intestinalis Mitosomes Using In Vivo Enzymatic Tagging","volume":"35","author":"E Martincov\u00e1","year":"2015","journal-title":"Mol Cell Biol"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0213594","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T19:47:56Z","timestamp":1588967276000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0213594"}},"subtitle":[],"editor":[{"given":"Rodney D.","family":"Adam","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,3,11]]},"references-count":67,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2019,3,11]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0213594","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,11]]}}}