{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T10:32:53Z","timestamp":1787740373635,"version":"build-2784847793"},"reference-count":51,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2019,4,3]],"date-time":"2019-04-03T00:00:00Z","timestamp":1554249600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Smithfield Foods and Murphy-Brown"},{"name":"USDA IPM Enhancement Grants"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0213601","type":"journal-article","created":{"date-parts":[[2019,4,3]],"date-time":"2019-04-03T17:30:31Z","timestamp":1554312631000},"page":"e0213601","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":11,"title":["Intracellular invasion and survival of Brucella neotomae, another possible zoonotic Brucella species"],"prefix":"10.1371","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8534-5002","authenticated-orcid":true,"given":"Steven Grant","family":"Waldrop","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9232-2888","authenticated-orcid":true,"given":"Nammalwar","family":"Sriranganathan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2019,4,3]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1186\/s40249-015-0086-5","article-title":"Narrative overview of animal and human brucellosis in Morocco: intensification of livestock production as a driver for emergence?","volume":"4","author":"MJ Ducrotoy","year":"2015","journal-title":"Infect Dis poverty. BioMed Central"},{"key":"ref2","unstructured":"Center for Disease Control and Prevention. CDC\u2014Home\u2014Brucellosis [Internet]. 2012 [cited 16 Mar 2016]. http:\/\/www.cdc.gov\/brucellosis\/index.html<\/ext-link>"},{"key":"ref3","unstructured":"Iowa State. Brucellosis. 2009; http:\/\/www.cfsph.iastate.edu\/Factsheets\/pdfs\/brucellosis.pdf<\/ext-link>"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1186\/1471-2148-10-249","article-title":"Genomic comparisons of Brucella spp. and closely related bacteria using base compositional and proteome based methods","volume":"10","author":"J Bohlin","year":"2010","journal-title":"BMC Evol Biol. BioMed Central"},{"key":"ref5","first-page":"116","article-title":"A Brucella spp. Isolate from a Pac-Man Frog (Ceratophrys ornata) Reveals Characteristics Departing from Classical Brucellae","volume":"6","author":"PF Soler-Llor\u00e9ns","year":"2016","journal-title":"Front Cell Infect Microbiol. Frontiers Media SA"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/S1473-3099(06)70382-6","article-title":"The new global map of human brucellosis","volume":"6","author":"G Pappas","year":"2006","journal-title":"Lancet Infect Dis"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"517","DOI":"10.3201\/eid1403.070829","article-title":"Marine Mammal Brucella<\/italic> Genotype Associated with Zoonotic Infection","volume":"14","author":"AM Whatmore","year":"2008","journal-title":"Emerg Infect Dis"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"997","DOI":"10.3201\/eid2306.162018","article-title":"Brucella neotomae Infection in Humans, Costa Rica","volume":"23","author":"M Su\u00e1rez-Esquivel","year":"2017","journal-title":"Emerg Infect Dis. Centers for Disease Control and Prevention"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1186\/s13256-017-1496-8","article-title":"Brucellosis caused by the wood rat pathogen Brucella neotomae: two case reports","volume":"11","author":"JM Villalobos-Vindas","year":"2017","journal-title":"J Med Case Rep. BioMed Central"},{"key":"ref10","unstructured":"LSU. Brucella species Presumptive Test Protocol: Biochemical Tests for Oxidase, Catalase, and Urease. LSU; 2009. pp. 1\u20135."},{"key":"ref11","article-title":"Promotion and Rescue of Intracellular Brucella neotomae<\/italic> Replication During Co-Infection With Legionella pneumophila<\/italic>","volume":"85","author":"Y-S Kang","year":"2017","journal-title":"Infect Immun"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0014112","article-title":"Identification of a Single-nucleotide insertion in the promoter region affecting the sodc promoter activity in brucella neotomae","volume":"5","author":"DA Moustafa","year":"2010","journal-title":"PLoS One"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1450","DOI":"10.3181\/0904-RM-124","article-title":"Brucella melitensis, B. neotomae and B. ovis elicit common and distinctive macrophage defense transcriptional responses","volume":"234","author":"J Covert","year":"2009","journal-title":"Exp Biol Med (Maywood). NIH Public Access"},{"key":"ref14","unstructured":"Dee TA. ORAL BRUCELLA NEOTOMAE STUDIES IN BISON, SWINE AND MICE [Internet]. Texas A&M Masters Thesis. 1990."},{"key":"ref15","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1016\/j.vaccine.2010.11.018","article-title":"Immunization of mice with gamma-irradiated Brucella neotomae and its recombinant strains induces protection against virulent B. abortus, B. melitensis, and B. suis challenge","volume":"29","author":"D Moustafa","year":"2011","journal-title":"Vaccine"},{"key":"ref16","article-title":"Brucella neotomae Recapitulates Attributes of Zoonotic Human Disease in a Murine Infection Model","volume":"87","author":"Yoon-Suk Kang","year":"2019","journal-title":"Infect Immun"},{"key":"ref17","first-page":"947","article-title":"A new species of Brucella isolated from the desert wood rat, Neotoma lepida Thomas","volume":"18","author":"HG STOENNER","year":"1957","journal-title":"Am J Vet Res"},{"key":"ref18","unstructured":"Cameron HS, Meyer ME. METABOLIC STUDIES ON BRUCELLA NEOTOMAE (STOENNER AND LACKMAN)."},{"key":"ref19","first-page":"376","article-title":"The behavior of Brucella neotomae and Brucella suis in reciprocal superinfection experiments in mice and guinea pigs","volume":"24","author":"HG STOENNER","year":"1963","journal-title":"Am J Vet Res"},{"key":"ref20","first-page":"9","article-title":"HOST-PARASITE RELATIONSHIPS WITH BRUCELLA NEOTOMAE","volume":"89","author":"IW GIBBY","year":"1965","journal-title":"J Bacteriol. American Society for Microbiology (ASM)"},{"key":"ref21","unstructured":"Turner KE. ATTEMPTED ORAL VACCINATION OF SWINE WITH Brucella neotoruae FOR PROTECTION AGAINST SUBSEQUENT CHALLENGE WITH Brucella suis. Texas A&M Masters Thesis. 1990;25: 62\u201368."},{"key":"ref22","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1016\/j.vetmic.2009.06.021","article-title":"Brucellosis: A re-emerging zoonosis","volume":"140","author":"MN Seleem","year":"2010","journal-title":"Vet Microbiol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"e84861","DOI":"10.1371\/journal.pone.0084861","article-title":"Entrance and Survival of Brucella pinnipedialis Hooded Seal Strain in Human Macrophages and Epithelial Cells","volume":"8","author":"AK Larsen","year":"2013","journal-title":"PLoS One. Public Library of Science"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"e107180","DOI":"10.1371\/journal.pone.0107180","article-title":"Oral immunization of mice with gamma-irradiated Brucella neotomae induces protection against intraperitoneal and intranasal challenge with virulent B. abortus 2308","volume":"9","author":"N Dabral","year":"2014","journal-title":"PLoS One"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"3484","DOI":"10.1128\/JCM.00837-08","article-title":"Evaluation of a multiplex PCR assay (Bruce-ladder) for molecular typing of all Brucella species, including the vaccine strains","volume":"46","author":"I L\u00f3pez-Go\u00f1i","year":"2008","journal-title":"J Clin Microbiol. American Society for Microbiology"},{"key":"ref26","unstructured":"J774A.1 ATCC \u00ae<\/sup> TIB-67\u2122<\/sup> Mus musculus ascites reticulum cell. In: ATCC [Internet]. [cited 4 Jan 2018]. https:\/\/www.atcc.org\/Products\/All\/TIB-67.aspx#culturemethod<\/ext-link>"},{"key":"ref27","unstructured":"HeLa S3 ATCC \u00ae<\/sup> CCL-2.2\u2122<\/sup> Homo sapiens cervix adenocarcinoma. In: ATCC [Internet]. [cited 4 Jan 2018]. https:\/\/atcc.org\/Products\/All\/CCL-2.2.aspx#culturemethod<\/ext-link>"},{"key":"ref28","article-title":"Bovine macrophage (BM) culture methods","author":"DS Lindsay","year":"2016"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"515","DOI":"10.3168\/jds.S0022-0302(95)76662-0","article-title":"Subcloning the MAC-T Bovine Mammary Epithelial Cell Line: Morphology, Growth Properties, and Cytogenetic Analysis of Clonal Cells","volume":"78","author":"B Zavizion","year":"1995","journal-title":"J Dairy Sci"},{"key":"ref30","unstructured":"DH82 ATCC \u00ae<\/sup> CRL-10389\u2122<\/sup> Canis familiaris malignant histiocyt. In: ATCC [Internet]. [cited 4 Jan 2018]. https:\/\/www.atcc.org\/Products\/All\/CRL-10389.aspx#culturemethod<\/ext-link>"},{"key":"ref31","unstructured":"THP-1 ATCC\u00ae<\/sup> TIB-202\u2122<\/sup>. In: ATCC [Internet]. [cited 4 Jan 2018]. https:\/\/www.atcc.org\/products\/all\/TIB-202.aspx#culturemethod<\/ext-link>"},{"key":"ref32","unstructured":"3D4\/31 ATCC \u00ae<\/sup> CRL-2844\u2122 Sus scrofa lung. In: ATCC [Internet]. [cited 4 Jan 2018]. https:\/\/www.atcc.org\/products\/all\/CRL-2844.aspx#culturemethod<\/ext-link>"},{"key":"ref33","first-page":"109","article-title":"The Integrins Involved in Soybean Agglutinin-Induced Cell Cycle Alterations in IPEC-J2","volume":"40","author":"L Pan","year":"2017","journal-title":"Mol Cells. Korean Society for Molecular and Cellular Biology"},{"key":"ref34","first-page":"200","article-title":"Biodefense Research Methodology and Animal Models","author":"JR Swearengen","year":"2012"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1111\/j.1574-6976.2012.00334.x","article-title":"Internal affairs: Investigating the Brucella intracellular lifestyle","volume":"36","author":"K von Bargen","year":"2012","journal-title":"FEMS Microbiol Rev"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"3244","DOI":"10.1128\/jb.179.10.3244-3249.1997","article-title":"Genome structure and phylogeny in the genus Brucella","volume":"179","author":"S Michaux-Charachon","year":"1997","journal-title":"J Bacteriol"},{"key":"ref37","first-page":"872","article-title":"Experimental infection of swine with Brucella neotomae","volume":"20","author":"GA BEAL","year":"1959","journal-title":"Am J Vet Res"},{"key":"ref38","first-page":"1524","article-title":"Delta-pgm, a new live-attenuated vaccine against Brucella suis","volume":"34","author":"C Czibener","year":"2016","journal-title":"Vaccine. Elsevier Ltd"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1007\/s00203-017-1360-7","article-title":"Omp31 plays an important role on outer membrane properties and intracellular survival of Brucella melitensis in murine macrophages and HeLa cells","volume":"199","author":"L Verdiguel-Fern\u00e1ndez","year":"2017","journal-title":"Arch Microbiol"},{"key":"ref40","unstructured":"CDC. Brucella suis Infection Associated with Feral Swine Hunting\u2014Three States, 2007\u20132008. In: Morbidity and Mortality Weekly Report [Internet]. 2009 [cited 4 Feb 2017]. https:\/\/www.cdc.gov\/mmwr\/preview\/mmwrhtml\/mm5822a3.htm<\/ext-link>"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1016\/j.vetimm.2012.12.001","article-title":"Monocyte-derived macrophages from Zebu (Bos taurus indicus) are more efficient to control Brucella abortus intracellular survival than macrophages from European cattle (Bos taurus taurus)","volume":"151","author":"AA Macedo","year":"2013","journal-title":"Vet Immunol Immunopathol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1111\/j.1574-695X.2002.tb00590.x","article-title":"Major histocompatibility complex class I and II expression on macrophages containing a virulent strain of Brucella abortus measured using green fluorescent protein-expressing brucellae and flow cytometry","volume":"33","author":"E Murphy","year":"2002","journal-title":"FEMS Immunol Med Microbiol"},{"key":"ref43","article-title":"Animal Brucellosis","author":"RJ Duncan","year":"1990"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1111\/j.1462-5822.2004.00372.x","article-title":"Adherence of Brucella to human epithelial cells and macrophages is mediated by sialic acid residues","volume":"6","author":"EI Castaneda-Roldan","year":"2004","journal-title":"Cell Microbiol. Wiley\/Blackwell (10.1111)"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.3168\/jds.S0022-0302(96)76454-8","article-title":"Staphylococcus aureus Invasion of Bovine Mammary Epithelial Cells","volume":"79","author":"RA Almeida","year":"1996","journal-title":"J Dairy Sci"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/j.micinf.2009.12.008","article-title":"Cholesterol, ganglioside GM1 and class A scavenger receptor contribute to infection by Brucella ovis and Brucella canis in murine macrophages","volume":"12","author":"AI Mart\u00edn-Mart\u00edn","year":"2010","journal-title":"Microbes Infect"},{"key":"ref47","doi-asserted-by":"crossref","DOI":"10.5772\/61463","article-title":"The Interaction Between Brucella and the Host Cell in Phagocytosis","author":"S Kim","year":"2015"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"e631","DOI":"10.1371\/journal.pone.0000631","article-title":"Brucella abortus Uses a Stealthy Strategy to Avoid Activation of the Innate Immune System during the Onset of Infection","volume":"2","author":"E Barquero-Calvo","year":"2007","journal-title":"PLoS One"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"1450","DOI":"10.3181\/0904-RM-124","article-title":"Brucella melitensis, B. neotomae and B. ovis elicit common and distinctive macrophage defense transcriptional responses","volume":"234","author":"J Covert","year":"2009","journal-title":"Exp Biol Med (Maywood). NIH Public Access"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4161\/spmg.19885","article-title":"Cell lines: Valuable tools or useless artifacts","volume":"2","author":"G Kaur","year":"2012","journal-title":"Spermatogenesis. Taylor & Francis"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/s13028-016-0188-5","article-title":"Brucella pinnipedialis in hooded seal (Cystophora cristata) primary epithelial cells","volume":"58","author":"AK Larsen","year":"2015","journal-title":"Acta Vet Scand. BioMed Central"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0213601","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T19:32:34Z","timestamp":1588966354000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0213601"}},"subtitle":[],"editor":[{"given":"Abdallah M.","family":"Samy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,4,3]]},"references-count":51,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2019,4,3]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0213601","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,3]]}}}