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R Foundation for Statistical Computing, Vienna, Austria. Version 3.4.4 [software]. 2018 Mar 15 [cited 2018 Aug 23]. <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/www.R-project.org\/\" xlink:type=\"simple\">https:\/\/www.R-project.org\/<\/ext-link>."},{"key":"ref31","unstructured":"Adams DC, Collyer ML, Kaliontzopoulou A, Sherratt E. Geomorph: Software for geometric morphometric analyses. R package version 3.0. 5."},{"key":"ref32","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1007\/BF02291478","article-title":"Generalized Procrustes analysis","volume":"40","author":"JC Gower","year":"1975","journal-title":"Psychometrika"},{"key":"ref33","article-title":"Geometric morphometrics for biologists: a primer","author":"ML Zelditch","year":"2012"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1111\/j.1096-3642.2010.00677.x","article-title":"Dealing with allometry in linear and geometric morphometrics: a taxonomic case study in the <italic>Leporinus cylindriformis<\/italic> group (Characiformes: Anostomidae) with description of a new species from Suriname","volume":"162","author":"BL Sidlauskas","year":"2011","journal-title":"Zoological Journal of the Linnean Society"},{"key":"ref35","unstructured":"Fruciano, C. GeometricMorphometricsMix: Miscellaneous functions useful for geometric morphometrics (R Package Version 0.0.5.0). <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/github.com\/fruciano\/GeometricMorphometricsMix\" xlink:type=\"simple\">https:\/\/github.com\/fruciano\/GeometricMorphometricsMix<\/ext-link>"},{"issue":"3","key":"ref36","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1007\/s00427-016-0537-4","article-title":"Measurement error in geometric morphometrics","volume":"226","author":"C Fruciano","year":"2016","journal-title":"Development Genes and Evolution"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"7034","DOI":"10.1002\/ece3.3256","article-title":"Sharing is caring? 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