{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T21:41:04Z","timestamp":1776289264989,"version":"3.50.1"},"reference-count":25,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2019,4,8]],"date-time":"2019-04-08T00:00:00Z","timestamp":1554681600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Universidad Europea de Madrid","award":["2015\/UEM21"],"award-info":[{"award-number":["2015\/UEM21"]}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["CP12\/03081"],"award-info":[{"award-number":["CP12\/03081"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI13\/01106"],"award-info":[{"award-number":["PI13\/01106"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0215068","type":"journal-article","created":{"date-parts":[[2019,4,8]],"date-time":"2019-04-08T17:26:45Z","timestamp":1554744405000},"page":"e0215068","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":33,"title":["Comparative performance evaluation of four commercial multiplex real-time PCR assays for the detection of the diarrhoea-causing protozoa Cryptosporidium hominis\/parvum, Giardia duodenalis and Entamoeba histolytica"],"prefix":"10.1371","volume":"14","author":[{"given":"Silvia","family":"Paulos","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Mar\u00eda","family":"Saugar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aida","family":"de Lucio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabel","family":"Fuentes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"Mateo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4015-8553","authenticated-orcid":true,"given":"David","family":"Carmena","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2019,4,8]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1016\/j.pcl.2017.03.006","article-title":"The burden and etiology of diarrheal illness in developing countries","volume":"64","author":"KL Kotloff","year":"2017","journal-title":"Pediatr Clin North Am"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1128\/CMR.05038-11","article-title":"Enteric protozoa in the developed world: a public health perspective","volume":"25","author":"SM Fletcher","year":"2012","journal-title":"Clin Microbiol Rev"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"190.e1-190","DOI":"10.1016\/j.cmi.2015.10.019","article-title":"Is real-time PCR-based diagnosis similar in performance to routine parasitological examination for the identification of <italic>Giardia intestinalis<\/italic>, <italic>Cryptosporidium parvum<\/italic>\/<italic>Cryptosporidium hominis<\/italic> and <italic>Entamoeba histolytica<\/italic> from stool samples? Evaluation of a new commercial multiplex PCR assay and literature review","volume":"22","author":"A Laude","year":"2016","journal-title":"Clin Microbiol Infect"},{"key":"ref4","unstructured":"Food and Agriculture Organization of the United Nations\/World Health Organization. Multicriteria-based ranking for risk management of food-borne parasites. Microbiological Risk Assessment Series, Rome 2014; 23: 302."},{"key":"ref5","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.watres.2017.01.036","article-title":"Waterborne transmission of protozoan parasites: Review of worldwide outbreaks\u2014An update 2011\u20132016","volume":"114","author":"A Efstratiou","year":"2017","journal-title":"Water Res"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1016\/j.ijheh.2017.03.008","article-title":"Water-borne protozoa parasites: The Latin American perspective","volume":"220","author":"FM Rosado-Garc\u00eda","year":"2017","journal-title":"Int J Hyg Environ Health"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1863","DOI":"10.1017\/S0031182014000419","article-title":"Application of PCR-based methods for diagnosis of intestinal parasitic infections in the clinical laboratory","volume":"141","author":"JJ Verweij","year":"2014","journal-title":"Parasitology"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1016\/j.cmi.2015.03.015","article-title":"Clinical consequences of new diagnostic tools for intestinal parasites","volume":"21","author":"L van Lieshout","year":"2015","journal-title":"Clin Microbiol Infect"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1016\/j.pt.2017.03.005","article-title":"New technologies for detection of enteric parasites","volume":"33","author":"U Ryan","year":"2017","journal-title":"Trends Parasitol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1097\/QCO.0b013e32833de0eb","article-title":"Newer diagnostic approaches to intestinal protozoa","volume":"23","author":"L van Lieshout","year":"2010","journal-title":"Curr Opin Infect Dis"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1128\/CMR.00122-13","article-title":"Molecular testing for clinical diagnosis and epidemiological investigations of intestinal parasitic infections","volume":"27","author":"JJ Verweij","year":"2014","journal-title":"Clin Microbiol Rev"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"3723","DOI":"10.1128\/JCM.02103-15","article-title":"Multiplex molecular panels for diagnosis of gastrointestinal infection: performance, result interpretation, and cost-effectiveness","volume":"53","author":"MJ Binnicker","year":"2015","journal-title":"J Clin Microbiol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.jinf.2013.04.009","article-title":"Systematic application of multiplex PCR enhances the detection of bacteria, parasites, and viruses in stool samples","volume":"67","author":"GN McAuliffe","year":"2013","journal-title":"J Infect"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"E458","DOI":"10.1111\/1469-0691.12255","article-title":"Simultaneous detection of gastrointestinal pathogens with a multiplex Luminex-based molecular assay in stool samples from diarrhoeic patients","volume":"19","author":"C Mengelle","year":"2013","journal-title":"Clin Microbiol Infect"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"188","DOI":"10.2143\/ACB.3170","article-title":"Detection of <italic>Giardia lamblia<\/italic>, <italic>Cryptosporidium<\/italic> spp. and <italic>Entamoeba histolytica<\/italic> in clinical stool samples by using multiplex real-time PCR after automated DNA isolation","volume":"68","author":"P Van Lint","year":"2013","journal-title":"Acta Clin Belg"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.diagmicrobio.2013.10.013","article-title":"Evaluation of the EasyScreen enteric parasite detection kit for the detection of <italic>Blastocystis<\/italic> spp., <italic>Cryptosporidium<\/italic> spp., <italic>Dientamoeba fragilis<\/italic>, <italic>Entamoeba complex<\/italic>, and <italic>Giardia intestinalis<\/italic> from clinical stool samples","volume":"78","author":"D Stark","year":"2014","journal-title":"Diagn Microbiol Infect Dis"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1128\/JCM.02674-14","article-title":"Multicenter evaluation of the BioFire FilmArray gastrointestinal panel for etiologic diagnosis of infectious gastroenteritis","volume":"53","author":"SN Buss","year":"2015","journal-title":"J Clin Microbiol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"e0159440","DOI":"10.1371\/journal.pone.0159440","article-title":"Evaluation of the NanoCHIP Gastrointestinal Panel (GIP) test for simultaneous detection of parasitic and bacterial enteric pathogens in fecal specimens","volume":"11","author":"S Ken Dror","year":"2016","journal-title":"PLoS One"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"2681","DOI":"10.1128\/JCM.00765-16","article-title":"Multicenter evaluation of BD Max enteric parasite real-time PCR assay for detection of <italic>Giardia duodenalis<\/italic>, <italic>Cryptosporidium hominis<\/italic>, <italic>Cryptosporidium parvum<\/italic>, and <italic>Entamoeba histolytica<\/italic>","volume":"54","author":"S Madison-Antenucci","year":"2016","journal-title":"J Clin Microbiol"},{"issue":"10","key":"ref20","doi-asserted-by":"crossref","first-page":"1722","DOI":"10.1515\/cclm-2017-1057","article-title":"Harmonization of PCR-based detection of intestinal pathogens: experiences from the Dutch external quality assessment scheme on molecular diagnosis of protozoa in stool samples","volume":"56","author":"TA Schuurs","year":"2018","journal-title":"Clin Chem Lab Med"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.mimet.2016.05.020","article-title":"Evaluation of five commercial methods for the extraction and purification of DNA from human faecal samples for downstream molecular detection of the enteric protozoan parasites <italic>Cryptosporidium<\/italic> spp., <italic>Giardia duodenalis<\/italic>, and <italic>Entamoeba<\/italic> spp","volume":"127","author":"S Paulos","year":"2016","journal-title":"J Microbiol Methods"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1111\/j.1469-0691.2007.01788.x","article-title":"Detection of diarrhoea-causing protozoa in general practice patients in The Netherlands by multiplex real-time PCR","volume":"13","author":"R ten Hove","year":"2007","journal-title":"Clin Microbiol Infect"},{"key":"ref23","article-title":"Evaluation of the Roche LightMix Gastro parasites multiplex PCR assay detecting <italic>Giardia duodenalis<\/italic>, <italic>Entamoeba histolytica<\/italic>, cryptosporidia, <italic>Dientamoeba fragilis<\/italic>, and <italic>Blastocystis hominis<\/italic>","author":"J Friesen","journal-title":"Clin Microbiol Infect"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1016\/j.cmi.2018.02.007","article-title":"Evaluation of a new multiplex PCR assay (ParaGENIE G-Amoeba Real-Time PCR kit) targeting <italic>Giardia intestinalis<\/italic>, <italic>Entamoeba histolytica<\/italic> and <italic>Entamoeba dispar<\/italic>\/<italic>Entamoeba moshkovskii<\/italic> from stool specimens: evidence for the limited performances of microscopy-based approach for amoeba species identification","volume":"24","author":"F Morio","year":"2018","journal-title":"Clin Microbiol Infect"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1016\/j.jinf.2014.11.009","article-title":"A cost benefit analysis of the Luminex xTAG Gastrointestinal Pathogen Panel for detection of infectious gastroenteritis in hospitalised patients","volume":"70","author":"SD Goldenberg","year":"2015","journal-title":"J Infect"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0215068","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,9]],"date-time":"2020-05-09T01:48:17Z","timestamp":1588988897000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0215068"}},"subtitle":[],"editor":[{"given":"Rodney D.","family":"Adam","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2019,4,8]]},"references-count":25,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2019,4,8]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0215068","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,8]]}}}