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Laboratory Methods\u2014BAM Media M147: Thiosulfate-Citrate-Bile Salts-Sucrose (TCBS) Agar [Internet]. [cited 23 Jul 2015]. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/www.fda.gov\/Food\/FoodScienceResearch\/LaboratoryMethods\/ucm063692.htm\" xlink:type=\"simple\">https:\/\/www.fda.gov\/Food\/FoodScienceResearch\/LaboratoryMethods\/ucm063692.htm<\/ext-link>"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1089\/fpd.2008.0120","article-title":"Multiplex PCR assay for detection and simultaneous differentiation of genotypes of <italic>Vibrio vulnificus<\/italic> biotype 1","volume":"5","author":"EB Warner","year":"2008","journal-title":"Foodborne Pathog Dis"},{"key":"ref45","first-page":"AEM.00333","article-title":"Characterizing the ecology of Vibrio in the Neuse River Estuary, North Carolina using heat shock protein 60 (hsp60) next-generation amplicon sequencing","author":"KJ Jesser","year":"2018","journal-title":"Appl Environ 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