{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:35:46Z","timestamp":1790696146412,"version":"4.1.0"},"reference-count":21,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2019,5,31]],"date-time":"2019-05-31T00:00:00Z","timestamp":1559260800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["DK085535"],"award-info":[{"award-number":["DK085535"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["DK085535"],"award-info":[{"award-number":["DK085535"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["DK085535"],"award-info":[{"award-number":["DK085535"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000900","name":"California Institute for Regenerative Medicine","doi-asserted-by":"publisher","award":["RT2-01985"],"award-info":[{"award-number":["RT2-01985"]}],"id":[{"id":"10.13039\/100000900","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["DK041301"],"award-info":[{"award-number":["DK041301"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0216326","type":"journal-article","created":{"date-parts":[[2019,5,31]],"date-time":"2019-05-31T17:33:56Z","timestamp":1559324036000},"page":"e0216326","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":17,"title":["Intestinal epithelial replacement by transplantation of cultured murine and human cells into the small intestine"],"prefix":"10.1371","volume":"14","author":[{"given":"Hassan A.","family":"Khalil","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung Noh","family":"Hong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua D.","family":"Rouch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Scott","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonghoon","family":"Cho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiafang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael S.","family":"Lewis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin G.","family":"Martin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James C. Y.","family":"Dunn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6988-847X","authenticated-orcid":true,"given":"Matthias G.","family":"Stelzner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2019,5,31]]},"reference":[{"issue":"8","key":"ref1","doi-asserted-by":"crossref","first-page":"2045","DOI":"10.1053\/j.gastro.2018.03.067","article-title":"Advances in Evaluation of Chronic Diarrhea in Infants","volume":"154","author":"JR Thiagarajah","year":"2018","journal-title":"Gastroenterology"},{"key":"ref2","first-page":"47","article-title":"Overview of pediatric short bowel syndrome","volume":"1","author":"D Duro","year":"2008","journal-title":"J Pediatr Gastroenterol Nutr"},{"issue":"2","key":"ref3","doi-asserted-by":"crossref","first-page":"666","DOI":"10.5966\/sctm.2016-0153","article-title":"Concise Review: The Potential Use of Intestinal Stem Cells to Treat Patients with Intestinal Failure.","volume":"6","author":"SN Hong","year":"2017","journal-title":"Stem Cells Transl Med"},{"issue":"6","key":"ref4","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1016\/j.stem.2013.09.015","article-title":"Transplantation of expanded fetal intestinal progenitors contributes to colon regeneration after injury","volume":"13","author":"RP Fordham","year":"2013","journal-title":"Cell Stem Cell"},{"issue":"4","key":"ref5","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1038\/nm.2695","article-title":"Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5(+) stem cell","volume":"18","author":"S Yui","year":"2012","journal-title":"Nat Med"},{"issue":"16","key":"ref6","doi-asserted-by":"crossref","first-page":"1752","DOI":"10.1101\/gad.245233.114","article-title":"Small intestinal stem cell identity is maintained with functional Paneth cells in heterotopically grafted epithelium onto the colon","volume":"28","author":"M Fukuda","year":"2014","journal-title":"Genes Dev"},{"issue":"5","key":"ref7","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1016\/j.jamcollsurg.2005.06.270","article-title":"Treatment of bile acid malabsorption using ileal stem cell transplantation","volume":"201","author":"JR Avansino","year":"2005","journal-title":"J Am Coll Surg"},{"issue":"3","key":"ref8","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1016\/j.surg.2006.03.012","article-title":"Orthotopic transplantation of intestinal mucosal organoids in rodents","volume":"140","author":"JR Avansino","year":"2006","journal-title":"Surgery"},{"issue":"11","key":"ref9","doi-asserted-by":"crossref","first-page":"e26898","DOI":"10.1371\/journal.pone.0026898","article-title":"Intestinal subepithelial myofibroblasts support in vitro and in vivo growth of human small intestinal epithelium","volume":"6","author":"N Lahar","year":"2011","journal-title":"PLoS One"},{"issue":"2","key":"ref10","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.stem.2017.11.012","article-title":"Reconstruction of the Human Colon Epithelium In Vivo.","volume":"22","author":"S Sugimoto","year":"2018","journal-title":"Cell Stem Cell"},{"issue":"9","key":"ref11","doi-asserted-by":"crossref","first-page":"e107814","DOI":"10.1371\/journal.pone.0107814","article-title":"Type I collagen as an extracellular matrix for the in vitro growth of human small intestinal epithelium","volume":"9","author":"Z Jabaji","year":"2014","journal-title":"PLoS One"},{"issue":"12","key":"ref12","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1089\/ten.tec.2012.0710","article-title":"Use of collagen gel as an alternative extracellular matrix for the in vitro and in vivo growth of murine small intestinal epithelium","volume":"19","author":"Z Jabaji","year":"2013","journal-title":"Tissue Eng Part C Methods"},{"issue":"1","key":"ref13","doi-asserted-by":"crossref","first-page":"e84651","DOI":"10.1371\/journal.pone.0084651","article-title":"Intestinal subepithelial myofibroblasts support the growth of intestinal epithelial stem cells","volume":"9","author":"NY Lei","year":"2014","journal-title":"PLoS One"},{"issue":"1","key":"ref14","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/s00441-016-2367-0","article-title":"A novel culture system for adult porcine intestinal crypts","volume":"365","author":"HA Khalil","year":"2016","journal-title":"Cell Tissue Res"},{"issue":"7244","key":"ref15","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1038\/nature07935","article-title":"Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche","volume":"459","author":"T Sato","year":"2009","journal-title":"Nature"},{"issue":"6","key":"ref16","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1016\/j.jpedsurg.2016.02.074","article-title":"Long-term renewable human intestinal epithelial stem cells as monolayers: A potential for clinical use","volume":"51","author":"A Scott","year":"2016","journal-title":"J Pediatr Surg"},{"issue":"1","key":"ref17","first-page":"81","article-title":"Controlled gene expression in primary Lgr5 organoid cultures","volume":"9","author":"BK Koo","year":"2011","journal-title":"Nat Methods"},{"issue":"11","key":"ref18","doi-asserted-by":"crossref","first-page":"2281","DOI":"10.1038\/nprot.2013.143","article-title":"Genome engineering using the CRISPR-Cas9 system","volume":"8","author":"FA Ran","year":"2013","journal-title":"Nat Protoc"},{"issue":"6","key":"ref19","doi-asserted-by":"crossref","first-page":"807","DOI":"10.2217\/rme.12.91","article-title":"Human tissue-engineered colon forms from postnatal progenitor cells: an in vivo murine model","volume":"7","author":"ER Barthel","year":"2012","journal-title":"Regen Med"},{"issue":"5","key":"ref20","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1007\/s11605-009-0806-x","article-title":"Intestinal stem cell organoid transplantation generates neomucosa in dogs","volume":"13","author":"VG Agopian","year":"2009","journal-title":"J Gastrointest Surg"},{"issue":"12","key":"ref21","doi-asserted-by":"crossref","first-page":"G1359","DOI":"10.1152\/ajpgi.00493.2011","article-title":"A nomenclature for intestinal in vitro cultures","volume":"302","author":"M Stelzner","year":"2012","journal-title":"Am J Physiol Gastrointest Liver Physiol"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0216326","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T18:47:32Z","timestamp":1588963652000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0216326"}},"subtitle":[],"editor":[{"given":"Michelina","family":"Plateroti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,5,31]]},"references-count":21,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2019,5,31]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0216326","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,5,31]]}}}