{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T17:09:34Z","timestamp":1787764174624,"version":"build-2784847793"},"reference-count":36,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2019,6,26]],"date-time":"2019-06-26T00:00:00Z","timestamp":1561507200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001655","name":"Deutscher Akademischer Austauschdienst","doi-asserted-by":"publisher","award":["E-20\/16"],"award-info":[{"award-number":["E-20\/16"]}],"id":[{"id":"10.13039\/501100001655","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/97540\/2013"],"award-info":[{"award-number":["SFRH\/BD\/97540\/2013"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Bundesanstalt f\u00fcr Materialforschung und -prufung","award":["Ideen_2013_90"],"award-info":[{"award-number":["Ideen_2013_90"]}]},{"name":"European Union","award":["PT2020 UID\/QUI\/50006\/2013"],"award-info":[{"award-number":["PT2020 UID\/QUI\/50006\/2013"]}]},{"DOI":"10.13039\/501100001655","name":"Deutscher Akademischer Austauschdienst","doi-asserted-by":"publisher","award":["E-20\/16"],"award-info":[{"award-number":["E-20\/16"]}],"id":[{"id":"10.13039\/501100001655","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0218686","type":"journal-article","created":{"date-parts":[[2019,6,26]],"date-time":"2019-06-26T17:37:55Z","timestamp":1561570675000},"page":"e0218686","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":19,"title":["Antibody conjugation to carboxyl-modified microspheres through N-hydroxysuccinimide chemistry for automated immunoassay applications: A general procedure"],"prefix":"10.1371","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6233-8133","authenticated-orcid":true,"given":"Peter","family":"Carl","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3049-0015","authenticated-orcid":true,"given":"In\u00eas I.","family":"Ramos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcela A.","family":"Segundo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2228-1248","authenticated-orcid":true,"given":"Rudolf J.","family":"Schneider","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2019,6,26]]},"reference":[{"issue":"11","key":"ref1","doi-asserted-by":"crossref","first-page":"1708","DOI":"10.1016\/j.chemosphere.2011.04.072","article-title":"Application of an ELISA to the quantification of carbamazepine in ground, surface and wastewaters and validation with LC-MS\/MS","volume":"84","author":"V Calisto","year":"2011","journal-title":"Chemosphere"},{"issue":"3","key":"ref2","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1007\/s00604-017-2081-y","article-title":"Ultrasensitive electrochemiluminescent salbutamol immunoassay with dual-signal amplification using CdSe@SiO2 as label and gold nanoparticles as substrate","volume":"184","author":"TT Dong","year":"2017","journal-title":"Microchim Acta"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/j.talanta.2016.08.078","article-title":"Simultaneous capture and sequential detection of two malarial biomarkers on magnetic microparticles","volume":"161","author":"CF Markwalter","year":"2016","journal-title":"Talanta"},{"issue":"3","key":"ref4","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1007\/s00216-017-0601-6","article-title":"Micro-bead injection spectroscopy for label-free automated determination of immunoglobulin G in human serum","volume":"410","author":"II Ramos","year":"2018","journal-title":"Anal Bioanal Chem"},{"issue":"9","key":"ref5","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1039\/b302786d","article-title":"Bead injection ELISA for the determination of antibodies implicated in type 1 diabetes mellitus","volume":"128","author":"AD Carroll","year":"2003","journal-title":"Analyst"},{"issue":"3\u20134","key":"ref6","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1007\/s00604-011-0705-1","article-title":"Particles and microfluidics merged: perspectives of highly sensitive diagnostic detection","volume":"176","author":"T Konry","year":"2012","journal-title":"Microchim Acta"},{"issue":"7","key":"ref7","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1016\/j.bios.2006.06.005","article-title":"Bead-based microfluidic immunoassays: The next generation","volume":"22","author":"CT Lim","year":"2007","journal-title":"Biosens Bioelectron"},{"issue":"3","key":"ref8","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1016\/j.talanta.2007.10.019","article-title":"Real sample analysis on microfluidic devices","volume":"74","author":"AG Crevillen","year":"2007","journal-title":"Talanta"},{"issue":"03","key":"ref9","first-page":"472","article-title":"An Overview of the Synthesis of Highly Versatile N-Hydroxysuccinimide Esters","volume":"49","author":"A Barr\u00e9","year":"2017","journal-title":"Synthesis"},{"issue":"10","key":"ref10","doi-asserted-by":"crossref","first-page":"4159","DOI":"10.1007\/s00604-017-2456-0","article-title":"Rapid single-step upconversion-linked immunosorbent assay for diclofenac","volume":"184","author":"A Hlavacek","year":"2017","journal-title":"Microchim Acta"},{"issue":"42","key":"ref11","doi-asserted-by":"crossref","first-page":"11043","DOI":"10.1021\/acs.langmuir.6b02591","article-title":"Improved Protein Conjugation with Uniform, Macroporous Poly(acrylamide-co-acrylic acid) Hydrogel Microspheres via EDC\/NHS Chemistry","volume":"32","author":"EY Liu","year":"2016","journal-title":"Langmuir"},{"issue":"5","key":"ref12","doi-asserted-by":"crossref","first-page":"1471","DOI":"10.1007\/s00604-017-2116-4","article-title":"Flow cytometric immunoassay for aflatoxin B1 using magnetic microspheres encoded with upconverting fluorescent nanocrystals","volume":"184","author":"Y Zhang","year":"2017","journal-title":"Microchim Acta"},{"issue":"2","key":"ref13","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.colsurfb.2009.02.011","article-title":"Optimized straight forward procedure for covalent surface immobilization of different biomolecules for single molecule applications","volume":"71","author":"R Janissen","year":"2009","journal-title":"Colloids Surf, B"},{"issue":"4\u20135","key":"ref14","doi-asserted-by":"crossref","first-page":"1703","DOI":"10.1016\/j.talanta.2010.03.027","article-title":"A chemometric approach for optimizing protein covalent immobilization on magnetic core-shell nanoparticles in view of an alternative immunoassay","volume":"81","author":"B Teste","year":"2010","journal-title":"Talanta"},{"issue":"10","key":"ref15","doi-asserted-by":"crossref","first-page":"1736","DOI":"10.1080\/01496395.2016.1174264","article-title":"Covalent immobilization of antibodies for the preparation of immunoaffinity chromatographic supports","volume":"51","author":"LA Mejia-Manzano","year":"2016","journal-title":"Sep Sci Technol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.molstruc.2015.02.038","article-title":"Adsorption and desorption studies of lysozyme by Fe3O4-polymer nanocomposite via fluorescence spectroscopy","volume":"1089","author":"K Koc","year":"2015","journal-title":"J Mol Struct"},{"issue":"1","key":"ref17","doi-asserted-by":"crossref","first-page":"907","DOI":"10.3390\/ijms16010907","article-title":"Development and Application of a Label-Free Fluorescence Method for Determining the Composition of Gold Nanoparticle-Protein Conjugates","volume":"16","author":"DV Sotnikov","year":"2015","journal-title":"Int J Mol Sci"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1520","DOI":"10.1126\/science.274.5292.1520","article-title":"Parallel Synthesis and Screening of a Solid Phase Carbohydrate Library","volume":"274","author":"R Liang","year":"1996","journal-title":"Science"},{"issue":"6","key":"ref19","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1021\/co500030f","article-title":"Utility of redundant combinatorial libraries in distinguishing high and lowquality screening hits","volume":"16","author":"TM Doran","year":"2014","journal-title":"ACS Comb Sci"},{"issue":"12","key":"ref20","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1016\/S1002-0721(12)60206-3","article-title":"Polystyrene microsphere-based lanthanide luminescent chemosensor for detection of organophosphate pesticides","volume":"30","author":"HX Zhang","year":"2012","journal-title":"J Rare Earths"},{"issue":"22","key":"ref21","doi-asserted-by":"crossref","first-page":"5358","DOI":"10.1039\/c2an35724k","article-title":"Colorimetric detection of copper ions in sub-micromolar concentrations using a triarylamine-linked resin bead","volume":"137","author":"TG Thomas","year":"2012","journal-title":"Analyst"},{"issue":"12","key":"ref22","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1007\/s40263-015-0289-0","article-title":"Intravenous and Intramuscular Formulations of Antiseizure Drugs in the Treatment of Epilepsy","volume":"29","author":"SI Patel","year":"2015","journal-title":"CNS Drugs"},{"issue":"12","key":"ref23","doi-asserted-by":"crossref","first-page":"3462","DOI":"10.1016\/j.envpol.2010.08.009","article-title":"Prioritizing research for trace pollutants and emerging contaminants in the freshwater environment","volume":"158","author":"KE Murray","year":"2010","journal-title":"Environ Pollut"},{"issue":"4","key":"ref24","first-page":"383","article-title":"Population pharmacokinetic of antiepileptic drugs in different populations","volume":"8","author":"JR Milovanovic","year":"2013","journal-title":"Cent Eur J Med"},{"issue":"38","key":"ref25","doi-asserted-by":"crossref","first-page":"6883","DOI":"10.1039\/C6AY01968D","article-title":"Improved strategies for selection and characterization of new monoclonal anti-carbamazepine antibodies during the screening process using feces and fluorescence polarization immunoassay","volume":"8","author":"L Oberleitner","year":"2016","journal-title":"Anal Methods"},{"issue":"1\u20132","key":"ref26","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/S0022-1759(99)00166-0","article-title":"A stable and highly sensitive 3,3\u2032,5,5\u2032-tetramethylbenzidine-based substrate reagent for enzyme-linked immunosorbent assays","volume":"233","author":"A Frey","year":"2000","journal-title":"J Immunol Methods"},{"issue":"19","key":"ref27","doi-asserted-by":"crossref","first-page":"12058","DOI":"10.1021\/la202267p","article-title":"Different EDC\/NHS Activation Mechanisms between PAA and PMAA Brushes and the Following Amidation Reactions","volume":"27","author":"C Wang","year":"2011","journal-title":"Langmuir"},{"issue":"2","key":"ref28","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1021\/la902220a","article-title":"Semiquantitative study of the EDC\/NHS activation of acid terminal groups at modified porous silicon surfaces","volume":"26","author":"S Sam","year":"2010","journal-title":"Langmuir"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1016\/j.ces.2015.07.031","article-title":"Kinetic modeling of protein PEGylation","volume":"137","author":"D Pfister","year":"2015","journal-title":"Chem Eng Sci"},{"issue":"3","key":"ref30","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1248\/bpb.32.523","article-title":"The pH-Dependent Formation of PEGylated Bovine Lactoferrin by Branched Polyethylene Glycol (PEG)-N-Hydroxysuccinimide (NHS) Active Esters","volume":"32","author":"Y Nojima","year":"2009","journal-title":"Biol Pharm Bull"},{"issue":"15","key":"ref31","doi-asserted-by":"crossref","first-page":"6443","DOI":"10.1039\/C5AY00042D","article-title":"Quantification of N-hydroxysuccinimide and N-hydroxysulfosuccinimide by hydrophilic interaction chromatography (HILIC)","volume":"7","author":"O Klykov","year":"2015","journal-title":"Anal Methods"},{"issue":"1","key":"ref32","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/0003-2697(86)90176-4","article-title":"Enhancement by N-hydroxysuccinimide of water soluble carbodiimide-mediated coupling reactions","volume":"156","author":"JV Staros","year":"1986","journal-title":"Anal Biochem"},{"issue":"12","key":"ref33","doi-asserted-by":"crossref","first-page":"1283","DOI":"10.1002\/jlcr.2580291204","article-title":"Synthesis of a bis(aminoethanethiol)ligand with an activated ester group for protein conjugation and TC-99M labeling","volume":"29","author":"M Eisenhut","year":"1991","journal-title":"J Label Compd Radiopharm"},{"issue":"12","key":"ref34","doi-asserted-by":"crossref","first-page":"1699","DOI":"10.1177\/002215540305101214","article-title":"Quantitative Comparison of Long-wavelength Alexa Fluor Dyes to Cy Dyes: Fluorescence of the Dyes and Their Bioconjugates","volume":"51","author":"JE Berlier","year":"2003","journal-title":"J Histochem Cytochem"},{"issue":"1\u20132","key":"ref35","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.aca.2007.02.035","article-title":"Miniaturization of environmental chemical assays in flowing systems: The lab-on-a-valve approach vis-a-vis lab-on-a-chip microfluidic devices","volume":"600","author":"M Miro","year":"2007","journal-title":"Anal Chim Acta"},{"issue":"3","key":"ref36","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1016\/j.talanta.2011.02.011","article-title":"Universal approach for mesofluidic handling of bead suspensions in lab-on-valve format","volume":"84","author":"HM Oliveira","year":"2011","journal-title":"Talanta"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0218686","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T19:04:48Z","timestamp":1588964688000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0218686"}},"subtitle":[],"editor":[{"given":"Sabato","family":"D'Auria","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,6,26]]},"references-count":36,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2019,6,26]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0218686","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,6,26]]}}}