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Relationship between insecticide sprays and huanglongbing progress in a citrus orchard in Sao Paulo, Brazil. Proceedings of the International Research Conference on Huanglongbing, Orlando, Florida, USA, December; 2008."},{"issue":"5","key":"ref13","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1094\/PHYTO-98-5-0592","article-title":"In planta distribution of \u2018Candidatus Liberibacter asiaticus\u2019 as revealed by polymerase chain reaction (PCR) and real-time PCR","volume":"98","author":"S Tatineni","year":"2008","journal-title":"Phytopathology"},{"issue":"3","key":"ref14","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.mcp.2007.12.006","article-title":"Distribution and quantification of Candidatus Liberibacter americanus, agent of huanglongbing disease of citrus in Sao Paulo State, Brasil, in leaves of an affected sweet orange tree as determined by PCR","volume":"22","author":"DC Teixeira","year":"2008","journal-title":"Molecular and Cellular Probes"},{"article-title":"editors. 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Ternary agrichemical compositions and related methods. Google Patents; 2018."},{"issue":"6","key":"ref28","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1071\/EA9910831","article-title":"Effectiveness of zinc sulfate and zinc chelate as foliar sprays in alleviating zinc deficiency of wheat grown on zinc-deficient soils in Western Australia","volume":"31","author":"R Brennan","year":"1991","journal-title":"Australian Journal of Experimental Agriculture"},{"key":"ref29","first-page":"91","article-title":"A response in spring cereals to foliar sprays of zinc in Ireland","author":"F MacNaeidhe","year":"1988","journal-title":"Irish Journal of Agricultural Research"},{"issue":"1","key":"ref30","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.carbpol.2003.11.007","article-title":"Preparation, characterization and antimicrobial activity of chitosan\u2013Zn complex","volume":"56","author":"X Wang","year":"2004","journal-title":"Carbohydrate polymers"},{"issue":"19","key":"ref31","doi-asserted-by":"crossref","first-page":"3527","DOI":"10.1016\/j.watres.2006.08.004","article-title":"Comparative eco-toxicity of nanoscale TiO2, SiO2, and ZnO water suspensions","volume":"40","author":"LK Adams","year":"2006","journal-title":"Water research"},{"issue":"1\u20133","key":"ref32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.colsurfa.2010.10.015","article-title":"Synthesis and characterization of zinc\/iron oxide composite nanoparticles and their antibacterial properties","volume":"374","author":"T Gordon","year":"2011","journal-title":"Colloids and Surfaces A: Physicochemical and Engineering Aspects"},{"issue":"21","key":"ref33","doi-asserted-by":"crossref","first-page":"2139021","DOI":"10.1063\/1.2742324","article-title":"Selective toxicity of zinc oxide nanoparticles to prokaryotic and eukaryotic systems","volume":"90","author":"KM Reddy","year":"2007","journal-title":"Applied physics letters"},{"issue":"2","key":"ref34","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/S0167-7012(03)00037-X","article-title":"Quantitative evaluation of antibacterial activities of metallic oxide powders (ZnO, MgO and CaO) by conductimetric assay","volume":"54","author":"J Sawai","year":"2003","journal-title":"Journal of microbiological methods"},{"issue":"5","key":"ref35","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/S0922-338X(98)80165-7","article-title":"Hydrogen peroxide as an antibacterial factor in zinc oxide powder slurry","volume":"86","author":"J Sawai","year":"1998","journal-title":"Journal of Fermentation and Bioengineering"},{"issue":"4","key":"ref36","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1021\/nl052326h","article-title":"Toxicological impact studies based on Escherichia coli bacteria in ultrafine ZnO nanoparticles colloidal medium","volume":"6","author":"R Brayner","year":"2006","journal-title":"Nano letters"},{"issue":"3","key":"ref37","first-page":"1407","article-title":"Nakagawa Z-e. Antibacterial activity of ZnO powder with crystallographic orientation","volume":"19","author":"T Ohira","year":"2008","journal-title":"Journal of Materials Science: Materials in Medicine"},{"issue":"2","key":"ref38","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.nano.2010.10.001","article-title":"Selective toxicity of ZnO nanoparticles toward Gram-positive bacteria and cancer cells by apoptosis through lipid peroxidation","volume":"7","author":"M Premanathan","year":"2011","journal-title":"Nanomedicine: Nanotechnology, Biology and Medicine"},{"issue":"6","key":"ref39","doi-asserted-by":"crossref","first-page":"1116","DOI":"10.1016\/j.tiv.2009.05.015","article-title":"Toxicity of nanoparticles of ZnO, CuO and TiO2 to yeast Saccharomyces cerevisiae","volume":"23","author":"K Kasemets","year":"2009","journal-title":"Toxicology in 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One"},{"issue":"12","key":"ref43","doi-asserted-by":"crossref","first-page":"3950","DOI":"10.1128\/jb.179.12.3950-3955.1997","article-title":"Characterization of transcription organization and analysis of unique expression patterns of an alkyl hydroperoxide reductase C gene (ahpC) and the peroxide regulator operon ahpF-oxyR-orfX from Xanthomonas campestris pv. phaseoli","volume":"179","author":"S Mongkolsuk","year":"1997","journal-title":"Journal of bacteriology"},{"key":"ref44","article-title":"Antimicrobial Zn-Based &quot;TSOL&quot; for Citrus Greening Management: Insights from Spectroscopy and Molecular Simulation","author":"SH Liu","year":"2019","journal-title":"Journal of agricultural and food chemistry"},{"issue":"2","key":"ref45","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1080\/00945718608057530","article-title":"Preparation, characterization and antimicrobial activity studies on some ternary complexes of Cu (II) with acetylacetone and various salicylic acids","volume":"16","author":"Y Anjaneyulu","year":"1986","journal-title":"Synthesis and Reactivity in Inorganic and Metal-Organic Chemistry"},{"issue":"3","key":"ref46","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1046\/j.1365-2958.1998.00797.x","article-title":"Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis","volume":"28","author":"GA O'toole","year":"1998","journal-title":"Molecular microbiology"},{"issue":"2","key":"ref47","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1094\/PHYTO-04-13-0100-R","article-title":"Foliar Application of Biofilm Formation\u2013Inhibiting Compounds Enhances Control of Citrus Canker Caused by Xanthomonas citri subsp. citri","volume":"104","author":"J Li","year":"2014","journal-title":"Phytopathology"},{"issue":"8","key":"ref48","doi-asserted-by":"crossref","first-page":"2690","DOI":"10.1128\/AEM.02649-06","article-title":"Assessing adhesion forces of type I and type IV pili of Xylella fastidiosa bacteria by use of a microfluidic flow chamber","volume":"73","author":"L De La Fuente","year":"2007","journal-title":"Applied and environmental microbiology"},{"key":"ref49","first-page":"9","article-title":"<italic>Liberibacter crescens<\/italic> biofilm formation in vitro: establishment of a model system for pathogenic &apos;<italic>Candidatus<\/italic> Liberibacter spp","author":"E Naranjo","year":"2019","journal-title":"Scientific Reports"},{"issue":"4","key":"ref50","doi-asserted-by":"crossref","first-page":"474","DOI":"10.21273\/JASHS.104.4.474","article-title":"Zinc concentration of pecan leaflets and yield as influenced by zinc source and adjuvants","volume":"104","author":"M Smith","year":"1979","journal-title":"J Amer Soc Hort Sci"},{"key":"ref51","unstructured":"\u201cMethod 3050B: Acid Digestion of Sediments, Sludges, and Soils,\u201d US EPA Washington, DC1996."},{"issue":"2","key":"ref52","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1038\/nprot.2007.521","article-title":"Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances","volume":"3","author":"I Wiegand","year":"2008","journal-title":"Nature protocols"},{"issue":"9","key":"ref53","doi-asserted-by":"crossref","first-page":"2324","DOI":"10.1128\/JCM.33.9.2324-2327.1995","article-title":"Colorimetric method for determining MICs of antimicrobial agents for Mycobacterium tuberculosis","volume":"33","author":"DM Yajko","year":"1995","journal-title":"Journal of clinical microbiology"},{"issue":"9","key":"ref54","doi-asserted-by":"crossref","first-page":"12347","DOI":"10.3390\/s120912347","article-title":"Multiple applications of Alamar Blue as an indicator of metabolic function and cellular health in cell viability bioassays","volume":"12","author":"SN Rampersad","year":"2012","journal-title":"Sensors"},{"issue":"5","key":"ref55","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1038\/nrmicro.2016.34","article-title":"Distinguishing between resistance, tolerance and persistence to antibiotic treatment","volume":"14","author":"A Brauner","year":"2016","journal-title":"Nature Reviews Microbiology"},{"issue":"6","key":"ref56","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1016\/j.mib.2004.10.014","article-title":"Biofilm formation in plant\u2013microbe associations","volume":"7","author":"BE Ramey","year":"2004","journal-title":"Current opinion in microbiology"},{"issue":"1","key":"ref57","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1146\/annurev.micro.56.012302.160705","article-title":"Biofilms as complex differentiated communities","volume":"56","author":"P Stoodley","year":"2002","journal-title":"Annual Reviews in Microbiology"},{"issue":"1","key":"ref58","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1094\/PHYTO-06-12-0138-R","article-title":"Heat treatment eliminates \u2018Candidatus Liberibacter asiaticus\u2019 from infected citrus trees under controlled conditions","volume":"103","author":"MT Hoffman","year":"2013","journal-title":"Phytopathology"},{"issue":"12","key":"ref59","doi-asserted-by":"crossref","first-page":"1495","DOI":"10.1094\/PHYTO-02-16-0114-R","article-title":"Evaluation of the spatiotemporal dynamics of oxytetracycline and its control effect against citrus Huanglongbing via trunk injection","volume":"106","author":"J Hu","year":"2016","journal-title":"Phytopathology"},{"issue":"9","key":"ref60","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.1094\/PHYTO-09-10-0262","article-title":"Chemical compounds effective against the citrus Huanglongbing bacterium \u2018Candidatus Liberibacter asiaticus\u2019 in planta","volume":"101","author":"M Zhang","year":"2011","journal-title":"Phytopathology"},{"issue":"2","key":"ref61","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1094\/PHYTO-05-17-0175-R","article-title":"Control of Citrus Huanglongbing via Trunk Injection of Plant Defense Activators and Antibiotics","volume":"108","author":"J Hu","year":"2017","journal-title":"Phytopathology"},{"issue":"1\u20102","key":"ref62","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1111\/j.1439-0418.2010.01592.x","article-title":"Repellency and toxicity of plant\u2010based essential oils and their constituents against Diaphorina citri Kuwayama (Hemiptera: Psyllidae)","volume":"136","author":"R Mann","year":"2012","journal-title":"Journal of applied entomology"},{"issue":"6","key":"ref63","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1111\/j.1439-0418.2010.01565.x","article-title":"Guava leaf volatiles and dimethyl disulphide inhibit response of Diaphorina citri Kuwayama to host plant volatiles","volume":"135","author":"E Onagbola","year":"2011","journal-title":"Journal of Applied Entomology"},{"key":"ref64","first-page":"651416","article-title":"Impacts of horticultural mineral oils and two insecticide practices on population fluctuation of Diaphorina citri and spread of huanglongbing in a citrus orchard in Sarawak","volume":"2012","author":"SCT Leong","year":"2012","journal-title":"The Scientific World Journal"},{"issue":"1","key":"ref65","doi-asserted-by":"crossref","DOI":"10.1093\/amt\/27.1.D8","article-title":"Efficacy of Surround for control of Asian citrus psyllid on citrus, 2000","volume":"27","author":"C McKenzie","year":"2002","journal-title":"Arthropod Management Tests"},{"issue":"3","key":"ref66","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1603\/0022-0493(2007)100[847:EOAPFO]2.0.CO;2","article-title":"Effects of a particle film on biology and behavior of Diaphorina citri (Hemiptera: Psyllidae) and its infestations in citrus","volume":"100","author":"DG Hall","year":"2007","journal-title":"Journal of Economic Entomology"},{"key":"ref67","unstructured":"FDA. PART 182\u2014SUBSTANCES GENERALLY RECOGNIZED AS SAFE 2018. Available from: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/www.ecfr.gov\/cgi-bin\/text-idx?SID=e956d645a8b4e6b3e34e4e5d1b690209&mc=true&node=pt21.3.182&rgn=div5#se21.3.182_18991\" xlink:type=\"simple\">https:\/\/www.ecfr.gov\/cgi-bin\/text-idx?SID=e956d645a8b4e6b3e34e4e5d1b690209&amp;mc=true&amp;node=pt21.3.182&amp;rgn=div5#se21.3.182_18991<\/ext-link>."},{"issue":"5","key":"ref68","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.1007\/s11947-012-0797-6","article-title":"Zinc oxide nanoparticles: synthesis, antimicrobial activity and food packaging applications","volume":"5","author":"PJP Espitia","year":"2012","journal-title":"Food and Bioprocess Technology"},{"key":"ref69","unstructured":"Quarles W. IPM for Asian citrus psyllid and huanglongbing disease. IPM Practitioner, XXXIV (1\/2). 2013."},{"key":"ref70","unstructured":"Santra S, Young M. Non-phytotoxic composite polymer film barrier as a repellent for controlling infection. Google Patents; 2018."},{"issue":"7","key":"ref71","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1016\/S1466-6049(01)00197-0","article-title":"Influence of particle size on the antibacterial activity of zinc oxide","volume":"3","author":"O Yamamoto","year":"2001","journal-title":"International Journal of Inorganic Materials"},{"issue":"3","key":"ref72","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1007\/s11051-006-9150-1","article-title":"Investigation into the antibacterial behaviour of suspensions of ZnO nanoparticles (ZnO nanofluids)","volume":"9","author":"L Zhang","year":"2007","journal-title":"Journal of Nanoparticle Research"},{"issue":"6","key":"ref73","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1002\/adfm.200801081","article-title":"Enhanced antibacterial activity of nanocrystalline ZnO due to increased ROS\u2010mediated cell injury","volume":"19","author":"G Applerot","year":"2009","journal-title":"Advanced Functional Materials"},{"issue":"3","key":"ref74","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1007\/BF02704255","article-title":"Synthesis, characterisation and electrochemical behaviour of Cu (II), Co (II), Ni (II) and Zn (II) complexes derived from acetylacetone andp-anisidine and their antimicrobial activity","volume":"115","author":"N Raman","year":"2003","journal-title":"Journal of Chemical sciences"},{"issue":"4","key":"ref75","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1007\/s12039-007-0041-5","article-title":"Synthesis, spectral characterization of Schiff base transition metal complexes: DNA cleavage and antimicrobial activity studies","volume":"119","author":"N Raman","year":"2007","journal-title":"Journal of Chemical sciences"},{"key":"ref76","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1146\/annurev-phyto-080516-035513","article-title":"The Candidatus Liberibacter\u2013host interface: insights into pathogenesis mechanisms and disease control","volume":"55","author":"N Wang","year":"2017","journal-title":"Annual review of phytopathology"},{"issue":"1","key":"ref77","first-page":"93","article-title":"The viable but nonculturable state in bacteria","volume":"43","author":"JD Oliver","year":"2005","journal-title":"The Journal of Microbiology"},{"key":"ref78","first-page":"277","article-title":"Nonculturable microorganisms in the environment","author":"JD Oliver","year":"2000"},{"issue":"4","key":"ref79","doi-asserted-by":"crossref","first-page":"1988","DOI":"10.1128\/AEM.68.4.1988-1993.2002","article-title":"Inducible gene expression by nonculturable bacteria in milk after pasteurization","volume":"68","author":"TS Gunasekera","year":"2002","journal-title":"Applied and environmental microbiology"},{"issue":"2","key":"ref80","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1111\/j.1574-6968.2009.01709.x","article-title":"Xanthomonas axonopodis pv. citri enters the VBNC state after copper treatment and retains its virulence","volume":"298","author":"R Del Campo","year":"2009","journal-title":"FEMS microbiology letters"},{"issue":"3","key":"ref81","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1139\/m79-063","article-title":"A tentative direct microscopic method for counting living marine bacteria","volume":"25","author":"K Kogure","year":"1979","journal-title":"Canadian Journal of Microbiology"},{"issue":"6","key":"ref82","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1128\/AEM.36.6.926-935.1978","article-title":"Simultaneous determination of the total number of aquatic bacteria and the number thereof involved in respiration","volume":"36","author":"R Zimmermann","year":"1978","journal-title":"Applied and Environmental Microbiology"},{"issue":"6","key":"ref83","doi-asserted-by":"crossref","first-page":"1801","DOI":"10.1128\/AEM.58.6.1801-1808.1992","article-title":"Use of a fluorescent redox probe for direct visualization of actively respiring bacteria","volume":"58","author":"G Rodriguez","year":"1992","journal-title":"Applied and Environmental Microbiology"},{"issue":"2","key":"ref84","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1128\/JCM.36.2.362-366.1998","article-title":"Rapid, low-technology MIC determination with clinical Mycobacterium tuberculosis isolates by using the microplate Alamar Blue assay","volume":"36","author":"SG Franzblau","year":"1998","journal-title":"Journal of clinical microbiology"},{"issue":"4","key":"ref85","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1562\/0031-8655(2002)0760385TESIOR2.0.CO2","article-title":"Encinas aV. The excited-state interaction of resazurin and resorufin with aminesin aqueous solutions. Photophysics and photochemical reaction","volume":"76","author":"C Bueno","year":"2002","journal-title":"Photochemistry and photobiology"},{"issue":"5","key":"ref86","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.1128\/AAC.41.5.1004","article-title":"Microplate alamar blue assay versus BACTEC 460 system for high-throughput screening of compounds against Mycobacterium tuberculosis and Mycobacterium avium","volume":"41","author":"L Collins","year":"1997","journal-title":"Antimicrobial agents and chemotherapy"},{"issue":"3","key":"ref87","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1252\/jcej.28.288","article-title":"Evaluation of growth inhibitory effect of ceramics powder slurry on bacteria by conductance method","volume":"28","author":"J Sawai","year":"1995","journal-title":"Journal of Chemical Engineering of Japan"},{"issue":"6","key":"ref88","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1016\/j.mib.2003.10.014","article-title":"Bacterial biofilms: prokaryotic adventures in multicellularity","volume":"6","author":"JS Webb","year":"2003","journal-title":"Current opinion in microbiology"},{"issue":"14","key":"ref89","doi-asserted-by":"crossref","first-page":"4427","DOI":"10.1128\/JB.186.14.4427-4440.2004","article-title":"Biofilms 2003: emerging themes and challenges in studies of surface-associated microbial life","volume":"186","author":"MR Parsek","year":"2004","journal-title":"Journal of bacteriology"},{"issue":"4","key":"ref90","doi-asserted-by":"crossref","first-page":"1140","DOI":"10.1128\/jb.184.4.1140-1154.2002","article-title":"Pseudomonas aeruginosa displays multiple phenotypes during development as a biofilm","volume":"184","author":"K Sauer","year":"2002","journal-title":"Journal of bacteriology"},{"issue":"6858","key":"ref91","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1038\/35101627","article-title":"Gene expression in Pseudomonas aeruginosa biofilms","volume":"413","author":"M Whiteley","year":"2001","journal-title":"Nature"},{"issue":"1","key":"ref92","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1128\/AEM.70.1.346-355.2004","article-title":"Frequency, size, and localization of bacterial aggregates on bean leaf surfaces","volume":"70","author":"J-M Monier","year":"2004","journal-title":"Applied and environmental microbiology"},{"issue":"1","key":"ref93","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1146\/annurev.phyto.41.022103.134521","article-title":"The ecological significance of biofilm formation by plant-associated bacteria","volume":"41","author":"CE Morris","year":"2003","journal-title":"Annual review of phytopathology"},{"key":"ref94","first-page":"141","article-title":"Microbial biofilms","author":"GV Bloemberg","year":"2004"},{"key":"ref95","first-page":"46","article-title":"Living in two worlds: the plant and insect lifestyles of Xylella fastidiosa","author":"S Chatterjee","year":"2008","journal-title":"Annual review of phytopathology"},{"issue":"6","key":"ref96","doi-asserted-by":"crossref","first-page":"e0130328","DOI":"10.1371\/journal.pone.0130328","article-title":"Asian citrus psyllid expression profiles suggest Candidatus Liberibacter asiaticus-mediated alteration of adult nutrition and metabolism, and of nymphal development and immunity","volume":"10","author":"M Vyas","year":"2015","journal-title":"PLoS One"},{"issue":"7","key":"ref97","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1094\/PHYTO-12-12-0331-RVW","article-title":"Citrus huanglongbing: a newly relevant disease presents unprecedented challenges","volume":"103","author":"N Wang","year":"2013","journal-title":"Phytopathology"},{"issue":"17","key":"ref98","doi-asserted-by":"crossref","first-page":"5269","DOI":"10.1128\/AEM.01412-16","article-title":"Natural competence of Xylella fastidiosa occurs at a high frequency inside microfluidic chambers mimicking the bacterium&apos;s natural habitats","volume":"82","author":"PP Kandel","year":"2016","journal-title":"Applied and environmental microbiology"},{"issue":"12","key":"ref99","doi-asserted-by":"crossref","first-page":"2161","DOI":"10.1007\/s11538-015-0115-3","article-title":"A Two-Dimensional Multiphase Model of Biofilm Formation in Microfluidic Chambers","volume":"77","author":"M Whidden","year":"2015","journal-title":"Bulletin of mathematical biology"},{"issue":"3","key":"ref100","doi-asserted-by":"crossref","first-page":"e0116903","DOI":"10.1371\/journal.pone.0116903","article-title":"Uptake and distribution of soil applied zinc by citrus trees\u2014addressing fertilizer use efficiency with 68Zn labeling","volume":"10","author":"FWR Hippler","year":"2015","journal-title":"PLoS One"},{"key":"ref101","unstructured":"Zink nitrate; MSDS No. S25901 [Online]; AquaPhoenix Scientific, Hanover, PA; 10.24.2014; <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/beta-static.fishersci.com\/content\/dam\/fishersci\/en_US\/documents\/programs\/education\/regulatory-documents\/sds\/chemicals\/chemicals-z\/S25901.pdf\" xlink:type=\"simple\">https:\/\/beta-static.fishersci.com\/content\/dam\/fishersci\/en_US\/documents\/programs\/education\/regulatory-documents\/sds\/chemicals\/chemicals-z\/S25901.pdf<\/ext-link>; (Accessed 4.9.2019)."},{"key":"ref102","unstructured":"Copper(II) sulfate; MSDS No. AD422870050 [Online]; Fisher Scientific, Fair Lawn, NJ; 4.9.2010; <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/www.fishersci.com\/msds?productName=AC422870050\" xlink:type=\"simple\">https:\/\/www.fishersci.com\/msds?productName=AC422870050<\/ext-link>; (Accessed 4.9.2019)."}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0218900","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,21]],"date-time":"2022-09-21T20:56:24Z","timestamp":1663793784000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0218900"}},"subtitle":[],"editor":[{"given":"Z. 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