{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T19:06:00Z","timestamp":1783191960324,"version":"3.54.6"},"reference-count":61,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2019,7,29]],"date-time":"2019-07-29T00:00:00Z","timestamp":1564358400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0220321","type":"journal-article","created":{"date-parts":[[2019,7,29]],"date-time":"2019-07-29T17:36:57Z","timestamp":1564421817000},"page":"e0220321","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":18,"title":["Internalin AB-expressing recombinant Lactobacillus casei protects Caco-2 cells from Listeria monocytogenes-induced damages under simulated intestinal conditions"],"prefix":"10.1371","volume":"14","author":[{"given":"Moloko G.","family":"Mathipa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arun K.","family":"Bhunia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6212-2770","authenticated-orcid":true,"given":"Mapitsi S.","family":"Thantsha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2019,7,29]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1139\/cjm-2012-0392","article-title":"How does <italic>Listeria monocytogenes<\/italic> combat acid conditions?","volume":"59","author":"JL Smith","year":"2012","journal-title":"Can J Miccrobiol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1962","DOI":"10.3389\/fmicb.2018.01962","article-title":"Food-Associated Stress Primes Foodborne Pathogens for the Gastrointestinal Phase of Infection","volume":"9","author":"N Horn","year":"2018","journal-title":"Front Microbiol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1038\/nrmicro1413","article-title":"<italic>Listeria monocytogenes<\/italic>: a multifaceted model","volume":"4","author":"M Hamon","year":"2006","journal-title":"Nat Rev Microbiol"},{"key":"ref4","first-page":"704321","article-title":"The opportunistic pathogen <italic>Listeria monocytogenes<\/italic>: pathogenicity and interaction with the mucosal immune system","volume":"2010","author":"M Schuppler","year":"2010","journal-title":"Int J Inflamation"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1016\/j.tim.2018.12.007","article-title":"Crossing the intestinal barrier via <italic>Listeria<\/italic> adhesion protein and internalin A","volume":"27","author":"R Drolia","year":"2019","journal-title":"Trends Microbiol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1016\/j.chom.2018.03.004","article-title":"<italic>Listeria<\/italic> adhesion protein induces intestinal epithelial barrier dysfunction for bacterial translocation","volume":"23","author":"R Drolia","year":"2018","journal-title":"Cell Host & Microbe"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"2782","DOI":"10.1099\/mic.0.036509-0","article-title":"LAP, an alcohol acetaldehyde dehydrogenase enzyme in <italic>Listeria<\/italic> promotes bacterial adhesion to enterocyte-like Caco-2 cells only in pathogenic species","volume":"156","author":"B Jagadeesan","year":"2010","journal-title":"Microbiology"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1016\/0092-8674(91)90009-N","article-title":"Entry of <italic>L<\/italic>. <italic>monocytogenes<\/italic> into cells is mediated by internalin, a repeat protein reminiscent of surface-antigens from Gram-positive cocci","volume":"65","author":"JL Gaillard","year":"1991","journal-title":"Cell"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1016\/S0092-8674(00)81070-3","article-title":"E-cadherin is the receptor for internalin, a surface protein required for entry of <italic>L<\/italic>. <italic>monocytogenes<\/italic> into epithelial cells","volume":"84","author":"J Mengaud","year":"1996","journal-title":"Cell"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1046\/j.1365-2958.1998.00750.x","article-title":"The InlB protein of <italic>Listeria monocytogenes<\/italic> is sufficient to promote entry into mammalian cells","volume":"27","author":"L Braun","year":"1998","journal-title":"Mol Microbiol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1111\/j.1348-0421.2010.00292.x","article-title":"Listerial invasion protein internalin B promotes entry into ileal Peyer&apos;s patches in vivo","volume":"55","author":"S Chiba","year":"2011","journal-title":"Microbiol Immunol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"3758","DOI":"10.1128\/IAI.66.8.3758-3766.1998","article-title":"Interactions of the invasive pathogens <italic>Salmonella typhimurium<\/italic>, <italic>Listeria monocytogenes<\/italic>, and <italic>Shigella flexneri<\/italic> with M cells and murine Peyer&apos;s patches","volume":"66","author":"VB Jensen","year":"1998","journal-title":"Infect Immun"},{"key":"ref13","first-page":"17","article-title":"Lactic acid bacteria as live vaccines","volume":"2","author":"A Mercenier","year":"2000","journal-title":"Curr Issues Mol Biol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.jviromet.2011.04.011","article-title":"Oral immunization with live <italic>Lactococcus lactis<\/italic> expressing rotavirus VP8* subunit induces specific immune response in mice","volume":"175","author":"B Marelli","year":"2011","journal-title":"J Virol Methods"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1007\/s12033-011-9450-2","article-title":"Development of vaccine delivery vehicles based on lactic acid bacteria","volume":"51","author":"S Tarahomjoo","year":"2012","journal-title":"Mol Biotechnol"},{"key":"ref16","unstructured":"FAO\/WHO (2002) Guidelines for the evaluation of probiotics in food. London, Ontario."},{"key":"ref17","first-page":"a010074","article-title":"Probiotics: properties, examples, and specific applications","volume":"3","author":"J Behnsen","year":"2013","journal-title":"Cold Spring Harbor Perspect Biol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/B978-0-12-394598-3.00005-8","article-title":"Modern approaches in probiotics research to control foodborne pathogens","volume":"67","author":"MAR Amalaradjou","year":"2012","journal-title":"Adv Food Nutr Res"},{"key":"ref19","first-page":"291","article-title":"Bioengineered probiotics, a strategic approach to control enteric infections","volume":"4","author":"MAR Amalaradjou","year":"2013","journal-title":"Bioengineered"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1136\/gut.52.7.975","article-title":"Double blind, placebo controlled trial of two probiotic strains in interleukin 10 knockout mice and mechanistic link with cytokine balance","volume":"52","author":"J McCarthy","year":"2003","journal-title":"Gut"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"e29277","DOI":"10.1371\/journal.pone.0029277","article-title":"Recombinant probiotic expressing <italic>Listeria<\/italic> adhesion protein attenuates <italic>Listeria monocytogenes<\/italic> virulence in vitro","volume":"7","author":"OK Koo","year":"2012","journal-title":"PLoS One"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1146\/annurev-food-030216-030256","article-title":"Engineered Probiotics: Applications and Biological Containment","volume":"8","author":"B Sola-Oladokun","year":"2017","journal-title":"Annu Rev Food Sci Technol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1111\/j.1348-0421.2005.tb03687.x","article-title":"<italic>Lactobacillus acidophilus<\/italic> expressing recombinant K99 adhesive fimbriae has an inhibitory effect on adhesion of enterotoxigenic <italic>Escherichia coli<\/italic>","volume":"49","author":"H Chu","year":"2005","journal-title":"Microbiol Immunol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1111\/j.1574-6968.2011.02244.x","article-title":"A flagellin-producing <italic>Lactococcus<\/italic> strain: interactions with mucin and enteropathogens","volume":"318","author":"B S\u00e1nchez","year":"2011","journal-title":"FEMS Microbiol Lett"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1111\/j.1574-695X.2007.00255.x","article-title":"Mice protected by oral immunization with <italic>Lactobacillus reuteri<\/italic> secreting fusion protein of <italic>Escherichia coli<\/italic> enterotoxin subunit protein","volume":"50","author":"C-M Wu","year":"2007","journal-title":"FEMS Immunol Med Microbiol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1111\/1751-7915.13407","article-title":"<italic>Lactobacillus casei<\/italic> expressing Internalins A and B reduces <italic>Listeria monocytogenes<\/italic> interaction with Caco\u20102 cells in vitro","volume":"12","author":"MG Mathipa","year":"2019","journal-title":"Microb Biotechnol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/S0378-5173(01)00897-3","article-title":"Simulated intestinal fluid as transport medium in the Caco-2 cell culture model","volume":"232","author":"F Ingels","year":"2002","journal-title":"Int J Pharm"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.ijpharm.2008.04.034","article-title":"Use of simulated intestinal fluid for Caco-2 permeability assay of lipophilic drugs","volume":"360","author":"L Fossati","year":"2008","journal-title":"Int J Pharm"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.ejpb.2006.08.010","article-title":"Absorption of poorly water soluble drugs subject to apical efflux using phospholipids as solubilizers in the Caco-2 cell model","volume":"66","author":"SB Kapitza","year":"2007","journal-title":"Eur J Pharm Biopharm"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.ejps.2007.08.002","article-title":"Development of simulated intestinal fluids containing nutrients as transport media in the Caco-2 cell culture model: assessment of cell viability, monolayer integrity and transport of a poorly aqueous soluble drug and a substrate of efflux mechanisms","volume":"32","author":"ML Lind","year":"2007","journal-title":"Eur J Pharm Sci"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1023\/A:1011910801212","article-title":"Evaluation of various dissolution media for predicting in vivo performance of class I and II drugs","volume":"15","author":"E Galia","year":"1998","journal-title":"Pharm Res"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.ijpharm.2004.01.014","article-title":"Effect of simulated intestinal fluid on drug permeability estimation across Caco-2 monolayers","volume":"274","author":"F Ingels","year":"2004","journal-title":"Int J Pharm"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1002\/jps.20553","article-title":"Intraluminal drug and formulation behavior and integration in in vitro permeability estimation: a case study with amprenavir","volume":"95","author":"J Brouwers","year":"2006","journal-title":"J Pharm Sci"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/S0076-6879(01)36602-8","article-title":"Lactobacilli as vehicles for targeting antigens to mucosal tissues by surface exposition of foreign antigens","volume":"336","author":"PH Pouwels","year":"2001","journal-title":"Methods Enzymol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1023\/A:1011984216775","article-title":"Dissolution testing as a prognostic tool for oral drug absorption: immediate release dosage forms","volume":"15","author":"JB Dressman","year":"1998","journal-title":"Pharm Res"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1016\/j.tiv.2012.03.009","article-title":"Good Caco-2 cell culture practices","volume":"26","author":"M Natoli","year":"2012","journal-title":"Toxicol In Vitro"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1056\/NEJMra1204186","article-title":"Designing Tomorrow&apos;s Vaccines","volume":"368","author":"GJ Nabel","year":"2013","journal-title":"N Engl J Med"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"2967","DOI":"10.1007\/s00253-015-6498-0","article-title":"Lactic acid bacteria\u201420 years exploring their potential as live vectors for mucosal vaccination","volume":"99","author":"A Wyszy\u0144ska","year":"2015","journal-title":"Appl Microbiol Biotechnol"},{"key":"ref39","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2017.01553","article-title":"Probiotic strain <italic>Lactobacillus casei<\/italic> BL23 prevents colitis-associated colorectal cancer","volume":"8","author":"E Jacouton","year":"2017","journal-title":"Front Immunol"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"2727","DOI":"10.1128\/AEM.01916-08","article-title":"Reduction of tumor necrosis factor alpha-inducing capacity of recombinant <italic>Lactobacillus casei<\/italic> via expression of <italic>Salmonella<\/italic> OmpC","volume":"75","author":"A Kajikawa","year":"2009","journal-title":"Appl Environ Microbiol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"585","DOI":"10.2217\/fmb.16.4","article-title":"Bioengineered probiotics as a new hope for health and diseases: an overview of potential and prospects","volume":"11","author":"M Kumar","year":"2016","journal-title":"Future Microbiol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/S0962-8924(02)00006-5","article-title":"Invasion of mammalian cells by <italic>Listeria monocytogenes<\/italic>: functional mimicry to subvert cellular functions","volume":"13","author":"P Cossart","year":"2003","journal-title":"Trends Cell Biol"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"9","DOI":"10.3389\/fcimb.2014.00009","article-title":"<italic>Listeria monocytogenes<\/italic>: survival and adaptation in the gastrointestinal tract","volume":"4","author":"C Gahan","year":"2014","journal-title":"Front Cell Infect Microbiol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"800","DOI":"10.1016\/j.addr.2013.01.003","article-title":"In vitro and in vivo models for the study of oral delivery of nanoparticles","volume":"65","author":"JM Gamboa","year":"2013","journal-title":"Adv Drug Delivary Rev"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1016\/j.micinf.2005.02.012","article-title":"Internalin-expressing <italic>Lactococcus lactis<\/italic> is able to invade small intestine of guinea pigs and deliver DNA into mammalian epithelial cells","volume":"7","author":"VD Guimaraes","year":"2005","journal-title":"Microbes Infect"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"6005","DOI":"10.1128\/AEM.68.12.6005-6012.2002","article-title":"Bile stress response in <italic>Listeria monocytogenes<\/italic> LO28: adaptation, cross-protection, and identification of genetic loci involved in bile resistance","volume":"68","author":"M Begley","year":"2002","journal-title":"Appl Environ Microbiol"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/S0168-1605(02)00404-X","article-title":"The effect of growth atmosphere on the ability of <italic>Listeria monocytogenes<\/italic> to survive exposure to acid, proteolytic enzymes and bile salts","volume":"84","author":"T King","year":"2003","journal-title":"Int J Food Microbiol"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"65","DOI":"10.4315\/0362-028X-70.1.65","article-title":"Effect of acid adaptation on growth during storage at 10 C and resistance to simulated gastric fluid of <italic>Listeria monocytogenes<\/italic> inoculated onto bologna formulated with or without antimicrobials","volume":"70","author":"G Formato","year":"2007","journal-title":"J Food Prot"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/S0168-1605(01)00432-9","article-title":"Inhibition of the binding of enterotoxigenic <italic>Escherichia coli<\/italic> Pb176 to human intestinal epithelial cell line HCT-8 by an extracellular protein fraction containing BIF of Bifidobacterium longum SBT2928: suggestive evidence of blocking of the binding receptor gangliotetraosylceramide on the cell surface","volume":"67","author":"S Fujiwara","year":"2001","journal-title":"Int J Food Microbiol"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.1093\/glycob\/10.11.1193","article-title":"<italic>Lactobacillus johnsonii<\/italic> La1 shares carbohydrate-binding specificities with several enteropathogenic bacteria","volume":"10","author":"JR Neeser","year":"2000","journal-title":"Glycobiology"},{"key":"ref51","first-page":"353","article-title":"Bioactive Foods in Promoting Health","author":"MC Collado","year":"2010"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"S101","DOI":"10.1079\/BJN2002635","article-title":"Competition for adhesion between probiotics and human gastrointestinal pathogens in the presence of carbohydrate","volume":"88","author":"YK Lee","year":"2002","journal-title":"Brit J Nutr"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1099\/jmm.0.05009-0","article-title":"Displacement of bacterial pathogens from mucus and Caco-2 cell surface by lactobacilli","volume":"52","author":"Y-K Lee","year":"2003","journal-title":"J Med Microbiol"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1016\/j.foodres.2005.10.003","article-title":"Adhesion and competitive inhibition and displacement of human enteropathogens by selected lactobacilli","volume":"39","author":"M Gueimonde","year":"2006","journal-title":"Food Res Int"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"5563","DOI":"10.1128\/AEM.00319-08","article-title":"Differences in survival among 13 <italic>Listeria monocytogenes<\/italic> strains in a dynamic model of the stomach and small intestine","volume":"74","author":"IM Barmpalia-Davis","year":"2008","journal-title":"Appl Environ Microbiol"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1128\/AEM.72.1.239-244.2006","article-title":"Effects of <italic>Lactococcus lactis<\/italic> on composition of intestinal microbiota: Role of nisin","volume":"72","author":"N Bernbom","year":"2006","journal-title":"Appl Environ Microbiol"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1128\/IAI.65.2.630-635.1997","article-title":"Critical roles of neutrophils in host defense against experimental systemic infections of mice by <italic>Listeria monocytogenes<\/italic>, <italic>Salmonella<\/italic> Typhimurium, and <italic>Yersinia enterocolitica<\/italic>","volume":"65","author":"JW Conlan","year":"1997","journal-title":"Infect Immun"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1128\/iai.28.2.516-523.1980","article-title":"Cell-mediated immunity to intestinal infection","volume":"28","author":"TT Macdonald","year":"1980","journal-title":"Infect Immun"},{"key":"ref59","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0021-9975(92)90090-H","article-title":"A microbiological, histopathological and immunohistological study of the intragastric inoculation of <italic>Listeria monocytogenes<\/italic> in mice","volume":"107","author":"AJ Marco","year":"1992","journal-title":"J Comp Pathol"},{"key":"ref60","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1128\/IAI.66.2.747-755.1998","article-title":"Comprehensive study of the intestinal stage of listeriosis in a rat ligated ileal loop system","volume":"66","author":"B Pron","year":"1998","journal-title":"Infect Immun"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1111\/j.1348-0421.1998.tb02290.x","article-title":"Systemic dissemination by intrarectal infection with <italic>Listeria monocytogenes<\/italic> in mice","volume":"42","author":"S Nishikawa","year":"1998","journal-title":"Microbiol Immunol"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0220321","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,24]],"date-time":"2022-09-24T20:20:13Z","timestamp":1664050813000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0220321"}},"subtitle":[],"editor":[{"given":"Milan","family":"Kojic","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,7,29]]},"references-count":61,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2019,7,29]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0220321","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,7,29]]}}}