{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T20:16:56Z","timestamp":1792441016395,"version":"4.5.0"},"reference-count":34,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2019,9,9]],"date-time":"2019-09-09T00:00:00Z","timestamp":1567987200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006668","name":"Fondo para la Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PICT2012-0145"],"award-info":[{"award-number":["PICT2012-0145"]}],"id":[{"id":"10.13039\/501100006668","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0221960","type":"journal-article","created":{"date-parts":[[2019,9,9]],"date-time":"2019-09-09T17:33:26Z","timestamp":1568050406000},"page":"e0221960","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":23,"title":["Isolation of five Enterobacteriaceae species harbouring blaNDM-1 and mcr-1 plasmids from a single paediatric patient"],"prefix":"10.1371","volume":"14","author":[{"given":"F.","family":"Martino","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Tijet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Melano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Petroni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Heinz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"De Belder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Faccone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Rapoport","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Biondi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Rodrigo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Vazquez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Pasteran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. R.","family":"Thomson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Corso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9106-1762","authenticated-orcid":true,"given":"S. A.","family":"Gomez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2019,9,9]]},"reference":[{"issue":"12","key":"pone.0221960.ref001","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1016\/j.tim.2011.09.005","article-title":"The emerging NDM carbapenemases","volume":"19","author":"P Nordmann","year":"2011","journal-title":"Trends in microbiology"},{"issue":"1","key":"pone.0221960.ref002","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1586\/14787210.2014.856756","article-title":"The spread and acquisition of NDM-1: a multifactorial problem","volume":"12","author":"AM Wailan","year":"2014","journal-title":"Expert Rev Anti Infect Ther"},{"issue":"6","key":"pone.0221960.ref003","doi-asserted-by":"crossref","first-page":"1725","DOI":"10.1093\/gbe\/evx115","article-title":"Genomic Epidemiology of NDM-1-Encoding Plasmids in Latin American Clinical Isolates Reveals Insights into the Evolution of Multidrug Resistance","volume":"9","author":"RA Marquez-Ortiz","year":"2017","journal-title":"Genome biology and evolution"},{"issue":"12","key":"pone.0221960.ref004","doi-asserted-by":"crossref","first-page":"7405","DOI":"10.1128\/AAC.01319-15","article-title":"Genetic contexts of blaNDM-1 in patients carrying multiple NDM-producing strains","volume":"59","author":"AM Wailan","year":"2015","journal-title":"Antimicrob Agents Chemother"},{"key":"pone.0221960.ref005","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.2147\/IDR.S175041","article-title":"Detection and characterization of a clinical Escherichia coli ST3204 strain coproducing NDM-16 and MCR-1","volume":"11","author":"X Li","year":"2018","journal-title":"Infect Drug Resist"},{"issue":"3","key":"pone.0221960.ref006","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1128\/JCM.01298-15","article-title":"Triton Hodge Test: Improved Protocol for Modified Hodge Test for Enhanced Detection of NDM and Other Carbapenemase Producers","volume":"54","author":"F Pasteran","year":"2016","journal-title":"J Clin Microbiol"},{"issue":"12","key":"pone.0221960.ref007","doi-asserted-by":"crossref","first-page":"3908","DOI":"10.1128\/JCM.02032-15","article-title":"Simplified Protocol for Carba NP Test for Enhanced Detection of Carbapenemase Producers Directly from Bacterial Cultures","volume":"53","author":"F Pasteran","year":"2015","journal-title":"J Clin Microbiol"},{"issue":"9","key":"pone.0221960.ref008","doi-asserted-by":"crossref","first-page":"1503","DOI":"10.3201\/eid1809.120355","article-title":"Rapid detection of carbapenemase-producing Enterobacteriaceae","volume":"18","author":"P Nordmann","year":"2012","journal-title":"Emerg Infect Dis"},{"issue":"6","key":"pone.0221960.ref009","doi-asserted-by":"crossref","first-page":"2467","DOI":"10.1128\/AAC.01615-12","article-title":"Differential distribution of plasmid-mediated quinolone resistance genes in clinical enterobacteria with unusual phenotypes of quinolone susceptibility from Argentina","volume":"57","author":"P Andres","year":"2013","journal-title":"Antimicrobial agents and chemotherapy"},{"issue":"2","key":"pone.0221960.ref010","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1006\/abio.1995.1220","article-title":"A general method for detecting and sizing large plasmids","volume":"226","author":"BM Barton","year":"1995","journal-title":"Anal Biochem"},{"issue":"14","key":"pone.0221960.ref011","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","article-title":"Prokka: rapid prokaryotic genome annotation","volume":"30","author":"T Seemann","year":"2014","journal-title":"Bioinformatics"},{"issue":"8","key":"pone.0221960.ref012","first-page":"e000083","article-title":"Robust high-throughput prokaryote de novo assembly and improvement pipeline for Illumina data","volume":"2","author":"AJ Page","year":"2016","journal-title":"Microb Genom"},{"issue":"9","key":"pone.0221960.ref013","doi-asserted-by":"crossref","first-page":"3146","DOI":"10.1099\/ijs.0.062737-0","article-title":"Description of Klebsiella quasipneumoniae sp. nov., isolated from human infections, with two subspecies, Klebsiella quasipneumoniae subsp. quasipneumoniae subsp. nov. and Klebsiella quasipneumoniae subsp. similipneumoniae subsp. nov., and demonstration that Klebsiella singaporensis is a junior heterotypic synonym of Klebsiella variicola","volume":"64","author":"S Brisse","year":"2014","journal-title":"Int J Syst Evol Microbiol"},{"issue":"27","key":"pone.0221960.ref014","doi-asserted-by":"crossref","first-page":"E3574","DOI":"10.1073\/pnas.1501049112","article-title":"Genomic analysis of diversity, population structure, virulence, and antimicrobial resistance in Klebsiella pneumoniae, an urgent threat to public health","volume":"112","author":"KE Holt","year":"2015","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"22","key":"pone.0221960.ref015","doi-asserted-by":"crossref","first-page":"3691","DOI":"10.1093\/bioinformatics\/btv421","article-title":"Roary: rapid large-scale prokaryote pan genome analysis","volume":"31","author":"AJ Page","year":"2015","journal-title":"Bioinformatics (Oxford, England)."},{"issue":"4","key":"pone.0221960.ref016","first-page":"e000056","article-title":"SNP-sites: rapid efficient extraction of SNPs from multi-FASTA alignments","volume":"2","author":"AJ Page","year":"2016","journal-title":"Microb Genom"},{"issue":"9","key":"pone.0221960.ref017","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.1093\/bioinformatics\/btu033","article-title":"RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies","volume":"30","author":"A Stamatakis","year":"2014","journal-title":"Bioinformatics (Oxford, England)."},{"key":"pone.0221960.ref018","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1186\/1471-2105-14-60","article-title":"Genome sequence-based species delimitation with confidence intervals and improved distance functions","volume":"14","author":"JP Meier-Kolthoff","year":"2013","journal-title":"BMC Bioinformatics"},{"issue":"7","key":"pone.0221960.ref019","doi-asserted-by":"crossref","first-page":"4082","DOI":"10.1128\/AAC.00368-16","article-title":"Mechanisms involved in acquisition of blaNDM genes by IncA\/C2 and IncFIIY plasmids","volume":"60","author":"AM Wailan","year":"2016","journal-title":"Antimicrob Agents Chemother"},{"issue":"7","key":"pone.0221960.ref020","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1089\/mdr.2017.0213","article-title":"Genetic diversity of KPC-producing Escherichia coli, Klebsiella oxytoca, Serratia marcescens, and Citrobacter freundii isolates from Argentina","volume":"24","author":"D De Belder","year":"2018","journal-title":"Microbial drug resistance"},{"key":"pone.0221960.ref021","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.plasmid.2018.02.002","article-title":"Compatibility and entry exclusion of IncA and IncC plasmids revisited: IncA and IncC plasmids are compatible","volume":"96\u201397","author":"SJ Ambrose","year":"2018","journal-title":"Plasmid"},{"issue":"4","key":"pone.0221960.ref022","doi-asserted-by":"crossref","first-page":"e01048","DOI":"10.1128\/AAC.01048-16","article-title":"Multiclonal expansion of Klebsiella pneumoniae isolates producing NDM-1 in Rio de Janeiro, Brazil","volume":"61","author":"CA Aires","year":"2017","journal-title":"Antimicrob Agents Chemother"},{"issue":"7","key":"pone.0221960.ref023","doi-asserted-by":"crossref","first-page":"e0180347","DOI":"10.1371\/journal.pone.0180347","article-title":"Molecular characteristics of mcr-1-carrying plasmids and new mcr-1 variant recovered from polyclonal clinical Escherichia coli from Argentina and Canada","volume":"12","author":"N Tijet","year":"2017","journal-title":"PLoS One"},{"issue":"10","key":"pone.0221960.ref024","doi-asserted-by":"crossref","first-page":"5951","DOI":"10.1128\/AAC.00027-15","article-title":"Phylogeny and comparative genomics unveil independent diversification trajectories of qnrB and genetic platforms within particular Citrobacter species","volume":"59","author":"TG Ribeiro","year":"2015","journal-title":"Antimicr Agents Chemother"},{"issue":"2","key":"pone.0221960.ref025","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1089\/mdr.2016.0033","article-title":"Prevalence of plasmid-pediated quinolone resistance genes in clinical enterobacteria from Argentina","volume":"23","author":"E Albornoz","year":"2017","journal-title":"Microbial drug resistance"},{"issue":"3","key":"pone.0221960.ref026","doi-asserted-by":"crossref","first-page":"1794","DOI":"10.1128\/AAC.02591-15","article-title":"Colistin-Nonsusceptible Pseudomonas aeruginosa Sequence Type 654 with blaNDM-1 Arrives in North America","volume":"60","author":"LF Mataseje","year":"2016","journal-title":"Antimicrob Agents Chemother"},{"issue":"3","key":"pone.0221960.ref027","doi-asserted-by":"crossref","first-page":"e89893","DOI":"10.1371\/journal.pone.0089893","article-title":"Sequential isolation in a patient of Raoultella planticola and Escherichia coli bearing a novel ISCR1 element carrying blaNDM-1","volume":"9","author":"J Li","year":"2014","journal-title":"PLoS One"},{"issue":"6","key":"pone.0221960.ref028","doi-asserted-by":"crossref","first-page":"3648","DOI":"10.1128\/AAC.04862-14","article-title":"Characterization of multiple NDM-1-producing Enterobacteriaceae isolates from the same patient","volume":"59","author":"N Tijet","year":"2015","journal-title":"Antimicrob Agents Chemother"},{"key":"pone.0221960.ref029","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.plasmid.2016.06.002","article-title":"pDGO100, a type 1 IncC plasmid from 1981 carrying ARI-A and a Tn1696-like transposon in a novel integrating element","volume":"86","author":"CJ Harmer","year":"2016","journal-title":"Plasmid"},{"issue":"6","key":"pone.0221960.ref030","doi-asserted-by":"crossref","first-page":"2227","DOI":"10.1128\/AAC.01707-08","article-title":"Resistance plasmid families in Enterobacteriaceae","volume":"53","author":"A Carattoli","year":"2009","journal-title":"Antimicrob Agents Chemother"},{"issue":"6","key":"pone.0221960.ref031","doi-asserted-by":"crossref","first-page":"e99209","DOI":"10.1371\/journal.pone.0099209","article-title":"Resistance determinants and mobile genetic elements of an NDM-1-encoding Klebsiella pneumoniae strain","volume":"9","author":"CM Hudson","year":"2014","journal-title":"PLoS One"},{"key":"pone.0221960.ref032","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.meegid.2018.10.020","article-title":"Characterization of a multidrug resistant Citrobacter amalonaticus clinical isolate harboring blaNDM-1 and mcr-1.5 genes","volume":"67","author":"D Faccone","year":"2018","journal-title":"Infect Genet Evol"},{"issue":"8","key":"pone.0221960.ref033","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1093\/cid\/ciw475","article-title":"World Health Organization ranking of antimicrobials according to their importance in human medicine: a critical step for developing risk management strategies to control antimicrobial resistance from food animal production","volume":"63","author":"PC Collignon","year":"2016","journal-title":"Clin Infect Dis"},{"issue":"5","key":"pone.0221960.ref034","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1016\/j.ijantimicag.2018.01.023","article-title":"The prevalence of colistin resistance in Escherichia coli and Klebsiella pneumoniae isolated from food animals in China: coexistence of mcr-1 and blaNDM with low fitness cost","volume":"51","author":"R Wang","year":"2018","journal-title":"Int J Antimicrob Agents"}],"updated-by":[{"DOI":"10.1371\/journal.pone.0224937","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2019,10,31]],"date-time":"2019-10-31T00:00:00Z","timestamp":1572480000000}}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0221960","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,9]],"date-time":"2020-05-09T07:14:04Z","timestamp":1589008444000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0221960"}},"subtitle":[],"editor":[{"given":"Ulrich","family":"N\u00fcbel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,9,9]]},"references-count":34,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2019,9,9]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0221960","relation":{"correction":[{"id-type":"doi","id":"10.1371\/journal.pone.0224937","asserted-by":"object"}]},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,9]]}}}