{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T13:10:27Z","timestamp":1792242627174,"version":"4.4.0"},"reference-count":66,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2019,9,17]],"date-time":"2019-09-17T00:00:00Z","timestamp":1568678400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0222291","type":"journal-article","created":{"date-parts":[[2019,9,17]],"date-time":"2019-09-17T17:25:32Z","timestamp":1568741132000},"page":"e0222291","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":76,"title":["Using DNA barcoding to improve invasive pest identification at U.S. ports-of-entry"],"prefix":"10.1371","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3898-125X","authenticated-orcid":true,"given":"Mary J. L.","family":"Madden","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6731-2506","authenticated-orcid":true,"given":"Robert G.","family":"Young","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John W.","family":"Brown","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott E.","family":"Miller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J.","family":"Frewin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert H.","family":"Hanner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2019,9,17]]},"reference":[{"issue":"1","key":"pone.0222291.ref001","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3391\/mbi.2014.5.1.01","article-title":"Tackling invasive alien species in Europe: the top 20 issues","volume":"5","author":"JM Caffrey","year":"2014","journal-title":"Manage Biol Invasions"},{"issue":"1","key":"pone.0222291.ref002","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1017\/S0021859605005708","article-title":"Crop losses to pests","volume":"144","author":"EC Oerke","year":"2006","journal-title":"J Agri Sci"},{"key":"pone.0222291.ref003","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1111\/epp.2600","article-title":"A Review of Pest Surveillance Techniques for Detecting Quarantine Pests in Europe","volume":"42","author":"S Augustin","year":"2012","journal-title":"EPPO Bulletin"},{"key":"pone.0222291.ref004","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.cropro.2013.10.022","article-title":"Crop losses and the economic impact of insect pests on Brazilian agriculture","volume":"56","author":"CM Oliveira","year":"2014","journal-title":"Crop Prot"},{"issue":"4","key":"pone.0222291.ref005","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1007\/s10530-007-9138-5","article-title":"The link between international trade and the global distribution of invasive alien species","volume":"10","author":"MI Westphal","year":"2008","journal-title":"Biol Invasions"},{"issue":"1\u20133","key":"pone.0222291.ref006","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1007\/s10493-009-9327-2","article-title":"Adventive eriophyoid mites: a global review of their impact, pathways, prevention and challenges","volume":"51","author":"D Navia","year":"2010","journal-title":"Exp Appl Acarol"},{"key":"pone.0222291.ref007","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1371\/journal.pone.0099786","article-title":"Effect of the internet commerce on dispersal modes of invasive alien species","volume":"9","author":"M Lenda","year":"2014","journal-title":"Plos One"},{"issue":"1","key":"pone.0222291.ref008","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1111\/epp.12462","article-title":"Identification of new pests likely to be introduced into Europe with the fruit trade","volume":"48","author":"M Suffert","year":"2018","journal-title":"EPPO Bulletin"},{"issue":"6","key":"pone.0222291.ref009","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1093\/jof\/107.6.320","article-title":"Impacts of Nonnative Invasive Species on US Forests and Recommendations for Policy and Management","volume":"107","author":"WK Moser","year":"2009","journal-title":"J Forest"},{"issue":"10","key":"pone.0222291.ref010","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0047689","article-title":"Gaps in Border Controls Are Related to Quarantine Alien Insect Invasions in Europe","volume":"7","author":"SJ Bacon","year":"2012","journal-title":"PLoS ONE"},{"issue":"5","key":"pone.0222291.ref011","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1017\/S0007485307005184","article-title":"How can alien species inventories and interception data help us prevent insect invasions?","volume":"97","author":"M Kenis","year":"2007","journal-title":"Bull Entomol Res"},{"key":"pone.0222291.ref012","doi-asserted-by":"crossref","first-page":"3353","DOI":"10.1016\/j.ecolmodel.2009.08.014","article-title":"Epidemiological risk assessment using linked network and grid based modelling: Phytophthora ramorum and Phytophthora kernoviae in the UK","volume":"220","author":"TD Harwood","year":"2009","journal-title":"Ecol Model"},{"key":"pone.0222291.ref013","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1094\/PHYTO-07-10-0192","article-title":"Networks in plant epidemiology: From genes to landscapes, countries, and continents","volume":"101","author":"M Moslonka-Lefebvre","year":"2011","journal-title":"Phytopathology"},{"key":"pone.0222291.ref014","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1111\/ddi.12056","article-title":"A method for designing complex biosecurity surveillance systems: detecting non-indigenous species of invertebrates on Barrow Island","volume":"19","author":"PJL Whittle","year":"2013","journal-title":"Divers and Distrib"},{"key":"pone.0222291.ref015","doi-asserted-by":"crossref","first-page":"96","DOI":"10.2137\/145960611795163033","article-title":"Recorded and potential alien invertebrate pests in Finnish agriculture and horticulture","volume":"20","author":"I Vanninen","year":"2011","journal-title":"Agri Food Sci"},{"issue":"3","key":"pone.0222291.ref016","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1098\/rsos.161002","article-title":"The wing pattern of Moerarchis Durrant, 1914 (Lepidoptera: Tineidae) clarifies transitions between predictive models","volume":"4","author":"SR Schachat","year":"2017","journal-title":"R Soc Open Sci"},{"issue":"1","key":"pone.0222291.ref017","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1899\/10-016.1","article-title":"Can DNA barcodes of stream macroinvertebrates improve descriptions of community structure and water quality?","volume":"30","author":"BW Sweeney","year":"2011","journal-title":"J N Am Benthol Soc"},{"key":"pone.0222291.ref018","volume-title":"Immature insects","author":"FW Stehr","year":"1987"},{"key":"pone.0222291.ref019","first-page":"1","article-title":"Identification of Heliothine (Lepidoptera: Noctuidae) Larvae Intercepted at U.S. Ports of Entry from the New World.","author":"TM Gilligan","year":"2019","journal-title":"J Econ Entomol"},{"key":"pone.0222291.ref020","first-page":"8","article-title":"Evaluating the accuracy of morphological identification of larval fishes by applying DNA barcoding","author":"HL Ko","year":"2013","journal-title":"Plos One"},{"key":"pone.0222291.ref021","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1007\/s10530-005-1798-4","article-title":"Interceptions of nonindigenous plant pests at US ports of entry and border crossings over a 17-year period","volume":"8","author":"DG McCullough","year":"2006","journal-title":"Biol Invasions"},{"key":"pone.0222291.ref022","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1111\/j.1365-2664.2006.01202.x","article-title":"Modelling global insect pest species assemblages to determine risk of invasion","volume":"43","author":"SP Worner","year":"2006","journal-title":"J Appl Ecol"},{"issue":"5","key":"pone.0222291.ref023","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1111\/j.1365-3059.2008.01886.x","article-title":"The biosecurity threat to the UK and global environment from international trade in plants","volume":"57","author":"M. Brasier","year":"2008","journal-title":"Plant Pathol"},{"key":"pone.0222291.ref024","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1098\/rspb.2002.2218","article-title":"Biological identifications through DNA barcodes","volume":"270","author":"PDN Hebert","year":"2003","journal-title":"Proc R Soc B"},{"issue":"18","key":"pone.0222291.ref025","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1079\/PAVSNNR20138018","article-title":"DNA barcoding for plant protection: applications and summary of available data for arthropod pests","volume":"8","author":"A Frewin","year":"2013","journal-title":"CAB Rev"},{"key":"pone.0222291.ref026","first-page":"33","article-title":"A new national unit for invasive species detection, assessment and eradication planning","volume":"109","author":"JRU Wilson","year":"2013","journal-title":"S Afr J Sci"},{"issue":"6","key":"pone.0222291.ref027","doi-asserted-by":"crossref","first-page":"e99546","DOI":"10.1371\/journal.pone.0099546","article-title":"Calibrating Snakehead Diversity with DNA Barcodes: Expanding Taxonomic Coverage to Enable Identification of Potential and Established Invasive Species","volume":"9","author":"NR Serrao","year":"2014","journal-title":"Plos One"},{"key":"pone.0222291.ref028","doi-asserted-by":"crossref","first-page":"60","DOI":"10.3923\/je.2010.60.79","article-title":"Identification of Sirex noctilio and Native North American Woodwasp Larvae using DNA Barcode","volume":"7","author":"AD Wilson","year":"2010","journal-title":"J Entomol"},{"key":"pone.0222291.ref029","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1002\/rra.2669","article-title":"Mass occurrence of bloodsucking blackflies in a regulated river reach: Localization of oviposition habitat of Simulium truncatum using DNA barcoding","volume":"30","author":"A Brabrand","year":"2014","journal-title":"River Res Appl"},{"key":"pone.0222291.ref030","doi-asserted-by":"crossref","first-page":"970","DOI":"10.1603\/EC13403","article-title":"Detection and genetic diversity of a heliothine invader (Lepidoptera: Noctuidae) from north and northeast of Brazil","volume":"107","author":"T Mastrangelo","year":"2014","journal-title":"J Econ Entomol"},{"key":"pone.0222291.ref031","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/j.aspen.2014.05.006","article-title":"Association of black fly (Diptera: Simuliidae) life stages using DNA barcode","volume":"17","author":"P Pramual","year":"2014","journal-title":"J Asia-Pac Entomol"},{"key":"pone.0222291.ref032","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1111\/j.1471-8286.2007.01678.x","article-title":"BOLD: The Barcode of Life Data System (http:\/\/www.barcodinglife.org)","volume":"7","author":"S Ratnasingham","year":"2007","journal-title":"Mol Ecol Notes"},{"key":"pone.0222291.ref033","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1111\/j.1755-0998.2009.02629.x","article-title":"The front-end logistics of DNA barcoding: Challenges and prospects","volume":"9","author":"AV Borisenko","year":"2009","journal-title":"Mol Ecol Resour"},{"key":"pone.0222291.ref034","author":"R. Hanner","year":"2009","journal-title":"Data Standards for BARCODE Records in INSDC (BRIs)"},{"issue":"1","key":"pone.0222291.ref035","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1086\/696613","article-title":"Identifying North American freshwater invertebrates using DNA barcodes: are existing COI sequence libraries fit for purpose?","volume":"37","author":"C. J. Curry","year":"2018","journal-title":"Freshwater Science"},{"issue":"9","key":"pone.0222291.ref036","doi-asserted-by":"crossref","first-page":"e0200177","DOI":"10.1371\/journal.pone.0200177","article-title":"Over 2.5 million COI sequences in GenBank and growing","volume":"13","author":"T. M. Porter","year":"2018","journal-title":"PloS one"},{"issue":"1444","key":"pone.0222291.ref037","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1098\/rstb.2003.1447","article-title":"The promise of a DNA taxonomy","volume":"359","author":"M.L. Blaxter","year":"2004","journal-title":"Philos.Trans. R. Soc. B Biol. Sci"},{"key":"pone.0222291.ref038","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0066213","article-title":"A DNA-Based Registry for All Animal Species: The Barcode Index Number (BIN) System","volume":"8","author":"S Ratnasingham","year":"2013","journal-title":"Plos One"},{"issue":"3","key":"pone.0222291.ref039","doi-asserted-by":"crossref","first-page":"e92797","DOI":"10.1371\/journal.pone.0092797","article-title":"A transcontinental challenge\u2014A test of DNA barcode performance for 1,541 species of Canadian Noctuoidea (Lepidoptera)","volume":"9","author":"RJ Zahiri","year":"2014","journal-title":"Plos One"},{"issue":"3","key":"pone.0222291.ref040","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.aspen.2010.04.003","article-title":"A rapid non-destructive DNA extraction method for insects and other arthropods","volume":"13","author":"MA Castalanelli","year":"2012","journal-title":"J Asia-Pac Entomol"},{"key":"pone.0222291.ref041","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1007\/978-1-61779-591-6_3","article-title":"DNA Barcodes for Insects","volume":"858","author":"JJ Wilson","year":"2012","journal-title":"Method Mol Biol"},{"issue":"s1","key":"pone.0222291.ref042","doi-asserted-by":"crossref","DOI":"10.1111\/j.1755-0998.2009.02630.x","article-title":"Express barcodes: racing from specimen to identification","volume":"9","author":"NV Ivanova","year":"2009","journal-title":"Mol Ecol Resour"},{"issue":"41","key":"pone.0222291.ref043","doi-asserted-by":"crossref","first-page":"14812","DOI":"10.1073\/pnas.0406166101","article-title":"Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator","volume":"101","author":"PDN Hebert","year":"2004","journal-title":"Proc Natl Acad Sci USA"},{"issue":"4","key":"pone.0222291.ref044","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1073\/pnas.0510466103","article-title":"DNA barcodes distinguish species of tropical Lepidoptera","volume":"103","author":"M Hajibabaei","year":"2006","journal-title":"Proceedings of the National Academy of Sciences"},{"issue":"1","key":"pone.0222291.ref045","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1111\/j.1365-2664.2008.01600.x","article-title":"Trade, transport and trouble: managing invasive species pathways in an era of globalization","volume":"46","author":"PE Hulme","year":"2009","journal-title":"J Appl Ecol"},{"key":"pone.0222291.ref046","doi-asserted-by":"crossref","first-page":"12485","DOI":"10.1038\/ncomms12485","article-title":"Global threats from invasive alien species in the twenty-first century and national response capacities","volume":"7","author":"R Early","year":"2016","journal-title":"Nat Commun"},{"issue":"7","key":"pone.0222291.ref047","first-page":"e1000417","volume":"8","author":"C Watts","year":"2019","journal-title":"DNA metabarcoding as a tool for invertebrate community monitoring: A case study comparison with conventional techniques. Austral Entomol"},{"key":"pone.0222291.ref048","doi-asserted-by":"crossref","unstructured":"Bonants PJM. Results of the EU Project QBOL, Focusing on DNA Barcoding of Quarantine Organisms, Added to an International Database (Q-Bank) on Identification of Plant Quarantine Pathogens and Relatives. In: Gullino M, Bonants P, editors. Detection and Diagnostics of Plant Pathogens. Plant Pathology in the 21st Century (Contributions to the 9th International Congress). Dordrecht: Springer; 2014. pp. 119\u2013133.","DOI":"10.1007\/978-94-017-9020-8_8"},{"issue":"5","key":"pone.0222291.ref049","doi-asserted-by":"crossref","first-page":"2996","DOI":"10.1002\/ece3.4757","article-title":"Incomplete estimates of genetic diversity within species: Implications for DNA barcoding","volume":"9","author":"JD Phillips","year":"2019","journal-title":"Ecol Evol"},{"key":"pone.0222291.ref050","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1742-9994-7-16","article-title":"The integrative future of taxonomy","volume":"7","author":"JM Padial","year":"2010","journal-title":"Front Zool"},{"key":"pone.0222291.ref051","doi-asserted-by":"crossref","first-page":"1835","DOI":"10.1098\/rstb.2005.1715","article-title":"Wedding biodiversity inventory of a large and complex Lepidoptera fauna with DNA barcoding","volume":"360","author":"DH Janzen","year":"2005","journal-title":"Philos Trans R Soc Lond B Biol Sci"},{"issue":"6","key":"pone.0222291.ref052","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1071\/IS12038","article-title":"What happens to the traditional taxonomy when a well-known tropical saturniid moth fauna is DNA barcoded?","volume":"26","author":"DH Janzen","year":"2012","journal-title":"Invertebr Syst"},{"key":"pone.0222291.ref053","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1111\/1755-0998.12038","article-title":"DNA barcoding and the taxonomy of Microgastrinae wasps (Hymenoptera, Braconidae): impacts after 8 years and nearly 20 000 sequences","volume":"13","author":"AM Smith","year":"2012","journal-title":"Mol Ecol Resour"},{"issue":"Database issue","key":"pone.0222291.ref054","doi-asserted-by":"crossref","first-page":"D34","DOI":"10.1093\/nar\/gki063","article-title":"GenBank","volume":"33","author":"DA Benson","year":"2005","journal-title":"Nucleic Acids Res"},{"issue":"4","key":"pone.0222291.ref055","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0035858","article-title":"A ranking system for reference libraries of DNA barcodes: Application to marine fish species from Portugal","volume":"7","author":"FO Costa","year":"2012","journal-title":"Plos One"},{"issue":"12","key":"pone.0222291.ref056","doi-asserted-by":"crossref","first-page":"e422","DOI":"10.1371\/journal.pbio.0030422","article-title":"DNA Barcoding: Error Rates Based on Comprehensive Sampling","volume":"3","author":"CP Meyer","year":"2005","journal-title":"PLOS Biology"},{"issue":"1","key":"pone.0222291.ref057","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/1742-9994-4-8","article-title":"Does the DNA barcoding gap exist?\u2013a case study in blue butterflies (Lepidoptera: Lycaenidae)","volume":"4","author":"M. Wiemers","year":"2007","journal-title":"Frontiers in zoology"},{"issue":"4","key":"pone.0222291.ref058","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1071\/IS11022","article-title":"Barcoding type specimens helps to identify synonyms and an unnamed new species in Eumunida Smith, 1883 (Decapoda: Eumunididae)","volume":"25","author":"N. Puillandre","year":"2011","journal-title":"Invertebrate Systematics"},{"key":"pone.0222291.ref059","first-page":"12","article-title":"DNA barcoding evaluation and implications for phylogenetic relationships in Lauraceae from China","author":"Z Liu","year":"2017","journal-title":"Plos One"},{"key":"pone.0222291.ref060","doi-asserted-by":"crossref","first-page":"e1000417","DOI":"10.1371\/journal.pbio.1000417","article-title":"Barcoding life to conserve biological diversity: Beyond the taxonomic imperative","volume":"8","author":"R Vernooy","year":"2010","journal-title":"PLoS Biol"},{"issue":"1","key":"pone.0222291.ref061","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/s10841-018-0111-7","article-title":"The importance of hidden diversity for insect conservation: a case study in hoverflies (the Merodon atratus complex, Syrphidae, Diptera).","volume":"23","author":"D Mili\u0107","year":"2019","journal-title":"J Insect Conserv"},{"issue":"11","key":"pone.0222291.ref062","first-page":"962","article-title":"Museum harvesting in major natural history collections","volume":"60","author":"V. Levesque-Beaudin","year":"2017","journal-title":"Genome"},{"key":"pone.0222291.ref063","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1098\/rsbl.2009.0848","article-title":"DNA barcodes for 1\/1000 of the animal kingdom","volume":"6","author":"PDN Hebert","year":"2009","journal-title":"Biol Lett"},{"key":"pone.0222291.ref064","first-page":"6","article-title":"A comprehensive DNA barcode library for the looper moths (Lepidoptera: Geometridae) of British Columbia, Canada","author":"JR deWaard","year":"2011","journal-title":"Plos One"},{"issue":"7","key":"pone.0222291.ref065","article-title":"A DNA 'barcode blitz': Rapid digitization and sequencing of a natural history collection.","volume":"8","author":"PDN Hebert","year":"2013","journal-title":"Plos One"},{"issue":"6","key":"pone.0222291.ref066","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1111\/1755-0998.12132","article-title":"A new set of primers for COI amplification from freshwater microcrustaceans","volume":"13","author":"S Prosser","year":"2013","journal-title":"Mol. Ecol. Resour"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0222291","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,23]],"date-time":"2024-07-23T16:31:53Z","timestamp":1721752313000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0222291"}},"subtitle":[],"editor":[{"given":"Massimo","family":"Labra","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,9,17]]},"references-count":66,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2019,9,17]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0222291","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,17]]}}}