{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T16:22:51Z","timestamp":1780935771070,"version":"3.54.1"},"reference-count":33,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2019,9,26]],"date-time":"2019-09-26T00:00:00Z","timestamp":1569456000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0222602","type":"journal-article","created":{"date-parts":[[2019,9,26]],"date-time":"2019-09-26T13:33:26Z","timestamp":1569504806000},"page":"e0222602","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":23,"title":["Human osteocyte expression of Nerve Growth Factor: The effect of Pentosan Polysulphate Sodium (PPS) and implications for pain associated with knee osteoarthritis"],"prefix":"10.1371","volume":"14","author":[{"given":"Catherine J. M.","family":"Stapledon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen","family":"Tsangari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucian B.","family":"Solomon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David G.","family":"Campbell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Plinio","family":"Hurtado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravi","family":"Krishnan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3123-9861","authenticated-orcid":true,"given":"Gerald J.","family":"Atkins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2019,9,26]]},"reference":[{"issue":"7","key":"pone.0222602.ref001","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1038\/nrrheum.2013.44","article-title":"Biologic agents in osteoarthritis: hopes and disappointments","volume":"9","author":"X Chevalier","year":"2013","journal-title":"Nat Rev Rheumatol"},{"issue":"1","key":"pone.0222602.ref002","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1007\/s11999-014-3716-5","article-title":"Preoperative pain and function profiles reflect consistent TKA patient selection among US surgeons","volume":"473","author":"DC Ayers","year":"2015","journal-title":"Clin Orthop Relat Res"},{"issue":"18","key":"pone.0222602.ref003","doi-asserted-by":"crossref","first-page":"E3632","DOI":"10.1073\/pnas.1701054114","article-title":"NGF-TrkA signaling in sensory nerves is required for skeletal adaptation to mechanical loads in mice","volume":"114","author":"RE Tomlinson","year":"2017","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"16","key":"pone.0222602.ref004","doi-asserted-by":"crossref","first-page":"1521","DOI":"10.1056\/NEJMoa0901510","article-title":"Tanezumab for the treatment of pain from osteoarthritis of the knee","volume":"363","author":"NE Lane","year":"2010","journal-title":"N Engl J Med"},{"issue":"6","key":"pone.0222602.ref005","doi-asserted-by":"crossref","first-page":"1202","DOI":"10.1136\/annrheumdis-2013-204905","article-title":"Efficacy and safety of tanezumab monotherapy or combined with non-steroidal anti-inflammatory drugs in the treatment of knee or hip osteoarthritis pain","volume":"74","author":"TJ Schnitzer","year":"2015","journal-title":"Ann Rheum Dis"},{"issue":"11","key":"pone.0222602.ref006","doi-asserted-by":"crossref","first-page":"2249","DOI":"10.3899\/jrheum.131294","article-title":"Efficacy and safety of intravenous tanezumab for the symptomatic treatment of osteoarthritis: 2 randomized controlled trials versus naproxen","volume":"41","author":"EF Ekman","year":"2014","journal-title":"J Rheumatol"},{"key":"pone.0222602.ref007","doi-asserted-by":"crossref","first-page":"5706359","DOI":"10.1155\/2016\/5706359","article-title":"Nerve Growth Factor Regulation by TNF-alpha and IL-1beta in Synovial Macrophages and Fibroblasts in Osteoarthritic Mice","volume":"2016","author":"S Takano","year":"2016","journal-title":"J Immunol Res"},{"key":"pone.0222602.ref008","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.jbior.2014.11.003","article-title":"NGF and ProNGF: Regulation of neuronal and neoplastic responses through receptor signaling","volume":"58","author":"RA Bradshaw","year":"2015","journal-title":"Adv Biol Regul"},{"key":"pone.0222602.ref009","doi-asserted-by":"crossref","first-page":"63","DOI":"10.3389\/fnmol.2016.00063","article-title":"NGF and proNGF Reciprocal Interference in Immunoassays: Open Questions, Criticalities, and Ways Forward","volume":"9","author":"F Malerba","year":"2016","journal-title":"Front Mol Neurosci"},{"issue":"10","key":"pone.0222602.ref010","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.1080\/14656566.2018.1491968","article-title":"An evaluation of the pharmacotherapy for interstitial cystitis","volume":"19","author":"LL Giusto","year":"2018","journal-title":"Expert Opin Pharmacother"},{"issue":"1","key":"pone.0222602.ref011","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1186\/s12891-017-1754-3","article-title":"Improved clinical outcome measures of knee pain and function with concurrent resolution of subchondral Bone Marrow Edema Lesion and joint effusion in an osteoarthritic patient following Pentosan Polysulphate Sodium treatment: a case report","volume":"18","author":"MJ Sampson","year":"2017","journal-title":"BMC Musculoskelet Disord"},{"key":"pone.0222602.ref012","unstructured":"Kumagai K, Shirabe S, Miyata N, Murata M, Yamauchi A, Kataoka Y, et al. Sodium pentosan polysulfate resulted in cartilage improvement in knee osteoarthritis\u2014an open clinical trial. (1472\u20136904 (Electronic))."},{"issue":"6","key":"pone.0222602.ref013","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1292\/jvms.11-0511","article-title":"Inhibitory effects of pentosan polysulfate sodium on MAP-kinase pathway and NF-kappaB nuclear translocation in canine chondrocytes in vitro","volume":"74","author":"T Sunaga","year":"2012","journal-title":"The Journal of veterinary medical science \/ the Japanese Society of Veterinary Science"},{"issue":"5","key":"pone.0222602.ref014","doi-asserted-by":"crossref","first-page":"e0177144","DOI":"10.1371\/journal.pone.0177144","article-title":"Pentosan polysulfate inhibits IL-1beta-induced iNOS, c-Jun and HIF-1alpha upregulation in canine articular chondrocytes","volume":"12","author":"EC Bwalya","year":"2017","journal-title":"PLoS One"},{"issue":"8","key":"pone.0222602.ref015","doi-asserted-by":"crossref","first-page":"2067","DOI":"10.1007\/s00198-012-1915-z","article-title":"Osteocyte regulation of bone mineral: a little give and take","volume":"23","author":"GJ Atkins","year":"2012","journal-title":"Osteoporos Int"},{"key":"pone.0222602.ref016","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.coph.2016.02.003","article-title":"Osteocytes: The master cells in bone remodelling","volume":"28","author":"M Prideaux","year":"2016","journal-title":"Curr Opin Pharmacol"},{"key":"pone.0222602.ref017","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.actbio.2016.01.016","article-title":"Evidence that osteocyte perilacunar remodelling contributes to polyethylene wear particle induced osteolysis","volume":"33","author":"RT Ormsby","year":"2016","journal-title":"Acta Biomater"},{"issue":"2","key":"pone.0222602.ref018","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00415-18","article-title":"Novel Insights into Staphylococcus aureus Deep Bone Infections: the Involvement of Osteocytes","volume":"9","author":"D Yang","year":"2018","journal-title":"MBio"},{"issue":"7","key":"pone.0222602.ref019","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1002\/jbmr.345","article-title":"Sclerostin is a locally acting regulator of late-osteoblast\/preosteocyte differentiation and regulates mineralization through a MEPE-ASARM-dependent mechanism","volume":"26","author":"GJ Atkins","year":"2011","journal-title":"J Bone Miner Res"},{"issue":"6","key":"pone.0222602.ref020","doi-asserted-by":"crossref","first-page":"C1358","DOI":"10.1152\/ajpcell.00216.2009","article-title":"Vitamin K promotes mineralization, osteoblast-to-osteocyte transition, and an anticatabolic phenotype by {gamma}-carboxylation-dependent and -independent mechanisms","volume":"297","author":"GJ Atkins","year":"2009","journal-title":"Am J Physiol Cell Physiol"},{"issue":"12","key":"pone.0222602.ref021","doi-asserted-by":"crossref","first-page":"2436","DOI":"10.1002\/jbmr.2003","article-title":"Sclerostin Regulates Release of Bone Mineral by Osteocytes by Induction of Carbonic Anhydrase 2","volume":"28","author":"M Kogawa","year":"2013","journal-title":"J Bone Miner Res"},{"key":"pone.0222602.ref022","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1016\/j.joca.2012.07.005","article-title":"Evidence for the dysregulated expression of TWIST1, TGF\u03b21 and SMAD3 in differentiating osteoblasts from primary hip osteoarthritis patients","volume":"20","author":"DD Kumarasinghe","year":"2012","journal-title":"Osteoarthritis & Cartilage"},{"issue":"10","key":"pone.0222602.ref023","doi-asserted-by":"crossref","first-page":"e25900","DOI":"10.1371\/journal.pone.0025900","article-title":"Sclerostin stimulates osteocyte support of osteoclast activity by a RANKL-dependent pathway","volume":"6","author":"AR Wijenayaka","year":"2011","journal-title":"PLoS One"},{"issue":"1","key":"pone.0222602.ref024","doi-asserted-by":"crossref","first-page":"R28","DOI":"10.1186\/ar2935","article-title":"Pentosan polysulfate promotes proliferation and chondrogenic differentiation of adult human bone marrow-derived mesenchymal precursor cells","volume":"12","author":"P Ghosh","year":"2010","journal-title":"Arthritis Res Ther"},{"key":"pone.0222602.ref025","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.bone.2016.04.017","article-title":"Isolation of osteocytes from human trabecular bone","volume":"88","author":"M Prideaux","year":"2016","journal-title":"Bone"},{"issue":"12","key":"pone.0222602.ref026","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.1093\/rheumatology\/41.12.1413","article-title":"Increased expression of nerve growth factor (NGF) and high affinity NGF receptor (p140 TrkA) in human osteoarthritic chondrocytes","volume":"41","author":"F Iannone","year":"2002","journal-title":"Rheumatology (Oxford)"},{"issue":"1","key":"pone.0222602.ref027","first-page":"634","article-title":"Blockage of tropomyosin receptor kinase a (TrkA) enhances chemo-sensitivity in breast cancer cells and inhibits metastasis in vivo","volume":"8","author":"J Zhang","year":"2015","journal-title":"Int J Clin Exp Med"},{"key":"pone.0222602.ref028","doi-asserted-by":"crossref","unstructured":"Rouillard AD, Gundersen GW, Fernandez NF, Wang Z, Monteiro CD, McDermott MG, et al. The harmonizome: a collection of processed datasets gathered to serve and mine knowledge about genes and proteins. LID\u201410.1093\/database\/baw100 [doi] LID\u2014baw100 [pii]. (1758\u20130463 (Electronic)).","DOI":"10.1093\/database\/baw100"},{"issue":"4","key":"pone.0222602.ref029","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1007\/s00198-008-0728-6","article-title":"Strontium ranelate treatment of human primary osteoblasts promotes an osteocyte-like phenotype while eliciting an osteoprotegerin response","volume":"20","author":"GJ Atkins","year":"2009","journal-title":"Osteoporos Int"},{"issue":"2","key":"pone.0222602.ref030","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1002\/jbmr.320","article-title":"The amazing osteocyte","volume":"26","author":"LF Bonewald","year":"2011","journal-title":"J Bone Miner Res"},{"issue":"3","key":"pone.0222602.ref031","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1007\/s002960050065","article-title":"Role of IL-1 beta and TNF-alpha in the regulation of NGF in experimentally induced arthritis in mice","volume":"18","author":"L Manni","year":"1998","journal-title":"Rheumatol Int"},{"key":"pone.0222602.ref032","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1016\/0049-3848(86)90169-6","article-title":"Absorption of heparin, LMW heparin and SP54 after subcutaneous injection, assessed by competitive binding assay","volume":"44","author":"J Dawes","year":"1986","journal-title":"Thrombosis Research"},{"key":"pone.0222602.ref033","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.neuroscience.2011.01.039","article-title":"The majority of myelinated and unmyelinated sensory nerve fibers that innervate bone express the tropomyosin receptor kinase A","volume":"178","author":"G Castaneda-Corral","year":"2011","journal-title":"Neuroscience"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0222602","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T19:32:31Z","timestamp":1588966351000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0222602"}},"subtitle":[],"editor":[{"given":"Dominique","family":"Heymann","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,9,26]]},"references-count":33,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2019,9,26]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0222602","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.736671650.793573707","asserted-by":"object"}]},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,26]]}}}