{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T14:05:29Z","timestamp":1792677929595,"version":"4.5.0"},"reference-count":51,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2019,9,30]],"date-time":"2019-09-30T00:00:00Z","timestamp":1569801600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0222775","type":"journal-article","created":{"date-parts":[[2019,9,30]],"date-time":"2019-09-30T18:24:35Z","timestamp":1569867875000},"page":"e0222775","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":23,"title":["Antifungal effects of a 1,3,4-thiadiazole derivative determined by cytochemical and vibrational spectroscopic studies"],"prefix":"10.1371","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5091-8846","authenticated-orcid":true,"given":"Barbara","family":"Chudzik","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Bonio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0449-2375","authenticated-orcid":true,"given":"Wojciech","family":"Dabrowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9814-5061","authenticated-orcid":true,"given":"Daniel","family":"Pietrzak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrzej","family":"Niewiadomy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alina","family":"Olender","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5559-8546","authenticated-orcid":true,"given":"Bo\u017cena","family":"Pawlikowska-Pawl\u0119ga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariusz","family":"Gago\u015b","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2019,9,30]]},"reference":[{"key":"pone.0222775.ref001","first-page":"14","article-title":"Emerging fungal infections in immunocompromised patients","volume":"3","author":"CY Low","year":"2011","journal-title":"F1000 Med Rep"},{"issue":"9","key":"pone.0222775.ref002","doi-asserted-by":"crossref","first-page":"2414","DOI":"10.1111\/j.1600-6143.2012.04100.x","article-title":"Donor-Derived Fungal Infections in Organ Transplant Recipients: Guidelines of the American Society of Transplantation, Infectious Diseases Community of Practice","volume":"12","author":"N Singh","year":"2012","journal-title":"American Journal of Transplantation"},{"key":"pone.0222775.ref003","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1111\/ajt.12118","article-title":"Emerging Fungal Infections in Solid Organ Transplantation.","volume":"13","author":"Practice AIDC","year":"2013","journal-title":"American Journal of Transplantation"},{"issue":"4","key":"pone.0222775.ref004","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1080\/13543776.2017.1261113","article-title":"Novel antifungal agents: a patent review (2013-present).","volume":"27","author":"MV Castelli","year":"2017","journal-title":"Expert Opinion on Therapeutic Patents"},{"issue":"9312","key":"pone.0222775.ref005","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1016\/S0140-6736(02)08162-X","article-title":"Antifungal drug resistance of pathogenic fungi","volume":"359","author":"DP Kontoyiannis","year":"2002","journal-title":"Lancet"},{"key":"pone.0222775.ref006","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1111\/j.1469-0691.2008.01978.x","article-title":"Changing epidemiology of systemic fungal infections","volume":"14","author":"M Richardson","year":"2008","journal-title":"Clinical Microbiology and Infection"},{"issue":"3","key":"pone.0222775.ref007","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1097\/MAJ.0b013e3181e99c88","article-title":"Opportunistic Fungi: A View to the Future.","volume":"340","author":"PG Pappas","year":"2010","journal-title":"American Journal of the Medical Sciences"},{"issue":"3","key":"pone.0222775.ref008","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.drup.2011.01.004","article-title":"Clinical breakpoints for the echinocandins and Candida revisited: Integration of molecular, clinical, and microbiological data to arrive at species-specific interpretive criteria","volume":"14","author":"MA Pfaller","year":"2011","journal-title":"Drug Resistance Updates"},{"issue":"8","key":"pone.0222775.ref009","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1586\/eri.09.67","article-title":"New triazoles and echinocandins: mode of action, in vitro activity and mechanisms of resistance","volume":"7","author":"MT Fera","year":"2009","journal-title":"Expert Review of Anti-Infective Therapy"},{"issue":"2\u20133","key":"pone.0222775.ref010","first-page":"93","article-title":"Fungal Diseases: the Last Frontier?","volume":"155","author":"MJ Day","year":"2016","journal-title":"J Comp Pathol"},{"issue":"5","key":"pone.0222775.ref011","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1111\/cbdd.12125","article-title":"1,3,4-Thiadiazole and its Derivatives: A Review on Recent Progress in Biological Activities. Chemical Biology &","volume":"81","author":"AK Jain","year":"2013","journal-title":"Drug Design"},{"key":"pone.0222775.ref012","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.2147\/DDDT.S155958","article-title":"2-Amino-1,3,4-thiadiazole as a potential scaffold for promising antimicrobial agents","volume":"12","author":"G Serban","year":"2018","journal-title":"Drug Design Development and Therapy"},{"issue":"1","key":"pone.0222775.ref013","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1002\/jhet.5570430108","article-title":"Synthesis of 5-substituted 2-(2,4-dihydroxyphenyl)-1,3,4-thiadiazoles","volume":"43","author":"J. Matysiak","year":"2006","journal-title":"Journal of Heterocyclic Chemistry"},{"issue":"6","key":"pone.0222775.ref014","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1134\/S1068162007060106","article-title":"2-(2,4-dihydroxyphenyl)-1,3,4-thiadiazole analogues: Antifungal activity in vitro against Candida species","volume":"33","author":"J Matysiak","year":"2007","journal-title":"Russian Journal of Bioorganic Chemistry"},{"issue":"4","key":"pone.0222775.ref015","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/j.ejmech.2005.12.007","article-title":"Synthesis and antiproliferative activity of some 5-substituted 2-(2,4-dihydroxyphenyl)-1,3,4-thiadiazoles","volume":"41","author":"J Matysiak","year":"2006","journal-title":"European Journal of Medicinal Chemistry"},{"issue":"7","key":"pone.0222775.ref016","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1002\/hc.20645","article-title":"Synthesis and Antifungal Activity of Novel 5-Substituted 4-(1,3,4-Thiadiazol-2-yl) benzene-1,3-Diols","volume":"21","author":"J Matysiak","year":"2010","journal-title":"Heteroatom Chemistry"},{"key":"pone.0222775.ref017","volume-title":"Approved standard","author":"CLSI","year":"2008"},{"key":"pone.0222775.ref018","volume-title":"CLSI document M38-A2","author":"CLSI","year":"2008"},{"issue":"2","key":"pone.0222775.ref019","doi-asserted-by":"crossref","first-page":"236","DOI":"10.4161\/auto.8.2.18600","article-title":"Autophagy-dependent senescence in response to DNA damage and chronic apoptotic stress","volume":"8","author":"K Singh","year":"2012","journal-title":"Autophagy"},{"key":"pone.0222775.ref020","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/S0091-679X(08)61717-3","article-title":"Lysosomal Proton Pump Activity\u2014Supravital Cell Staining with Acridine-Orange Differentiates Leukocyte Subpopulations","volume":"41","author":"F Traganos","year":"1994","journal-title":"Methods in Cell Biology"},{"issue":"13","key":"pone.0222775.ref021","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1002\/(SICI)1097-0061(19980930)14:13<1159::AID-YEA317>3.0.CO;2-3","article-title":"A spheroplast rate assay for determination of cell wall integrity in yeast","volume":"14","author":"R Ovalle","year":"1998","journal-title":"Yeast"},{"issue":"5","key":"pone.0222775.ref022","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1021\/jf60075a005","article-title":"Spectrophotometric semi-microdetermination of ergosterol in yeast","volume":"5","author":"J.L. Breivik ONO","year":"1957","journal-title":"Jour Agric And Food Chem"},{"issue":"10","key":"pone.0222775.ref023","doi-asserted-by":"crossref","first-page":"3332","DOI":"10.1128\/JCM.37.10.3332-3337.1999","article-title":"Quantitation of ergosterol content: Novel method for determination of fluconazole susceptibility of Candida albicans","volume":"37","author":"BA Arthington-Skaggs","year":"1999","journal-title":"Journal of Clinical Microbiology"},{"issue":"12","key":"pone.0222775.ref024","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1074\/mcp.M200062-MCP200","article-title":"Sequential fractionation and two-dimensional gel analysis unravels the complexity of the dimorphic fungus Candida albicans cell wall proteome","volume":"1","author":"A Pitarch","year":"2002","journal-title":"Mol Cell Proteomics"},{"issue":"4","key":"pone.0222775.ref025","doi-asserted-by":"crossref","first-page":"1876","DOI":"10.1002\/app.20647","article-title":"Chitin characterization by SEM, FTIR, XRD, and C-13 cross polarization\/mass angle spinning NMR","volume":"93","author":"G Cardenas","year":"2004","journal-title":"J Appl Polym Sci"},{"issue":"4","key":"pone.0222775.ref026","doi-asserted-by":"crossref","first-page":"2496","DOI":"10.1016\/j.carbpol.2011.11.031","article-title":"Yeast beta(1\u20133),(1\u20136)-D-glucan films: Preparation and characterization of some structural and physical properties","volume":"87","author":"M Novak","year":"2012","journal-title":"Carbohyd Polym"},{"issue":"4","key":"pone.0222775.ref027","first-page":"1129","article-title":"The Study of Infrared Spectrum of Chitin and Chitosan Extract as Potential Sources of Biomass","volume":"10","author":"P Negrea","year":"2015","journal-title":"Dig J Nanomater Bios"},{"issue":"4","key":"pone.0222775.ref028","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1016\/j.carbpol.2004.08.004","article-title":"Structure analysis and degree of substitution of chitin, chitosan and dibutyrylchitin by FT-IR spectroscopy and solid state C-13 NMR","volume":"58","author":"K Van de Velde","year":"2004","journal-title":"Carbohyd Polym"},{"issue":"4","key":"pone.0222775.ref029","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1007\/s00203-006-0094-8","article-title":"FTIR spectroscopy as a potential tool to analyse structural modifications during morphogenesis of Candida albicans","volume":"185","author":"I Adt","year":"2006","journal-title":"Archives of Microbiology"},{"issue":"3","key":"pone.0222775.ref030","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/S0144-8617(98)00099-X","article-title":"Microbial (1 -> 3)-beta-D-glucans, their preparation, physico-chemical characterization and immunomodulatory activity","volume":"38","author":"J Sandula","year":"1999","journal-title":"Carbohyd Polym"},{"issue":"4","key":"pone.0222775.ref031","doi-asserted-by":"crossref","first-page":"2585","DOI":"10.3892\/mmr.2014.3088","article-title":"Fourier transform infrared microspectroscopy monitoring of 5-fluorouracil-induced apoptosis in SW620 colon cancer cells","volume":"11","author":"YF Gao","year":"2015","journal-title":"Molecular Medicine Reports"},{"issue":"8","key":"pone.0222775.ref032","first-page":"724","article-title":"Li+ effect on the cell wall of the yeast Saccharomyces cerevisiae as probed by FT-IR spectroscopy","volume":"8","author":"A Zimkus","year":"2013","journal-title":"Central European Journal of Biology"},{"issue":"5","key":"pone.0222775.ref033","doi-asserted-by":"crossref","first-page":"1779","DOI":"10.1007\/s00216-006-0850-2","article-title":"A synchrotron FTIR microspectroscopy investigation of fungal hyphae grown under optimal and stressed conditions","volume":"387","author":"A Szeghalmi","year":"2007","journal-title":"Analytical and Bioanalytical Chemistry"},{"issue":"2","key":"pone.0222775.ref034","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1111\/j.1574-6968.2001.tb10601.x","article-title":"FTIR spectroscopic analysis of Saccharomyces cerevisiae cell walls: study of an anomalous strain exhibiting a pink-colored cell phenotype","volume":"197","author":"A Galichet","year":"2001","journal-title":"Fems Microbiology Letters"},{"issue":"5","key":"pone.0222775.ref035","doi-asserted-by":"crossref","DOI":"10.1093\/femsyr\/foy053","article-title":"Role of protein phosphomannosylation in the Candida tropicalis-macrophage interaction","volume":"18","author":"MJ Hernandez-Chavez","year":"2018","journal-title":"Fems Yeast Res"},{"issue":"1\u20132","key":"pone.0222775.ref036","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/S1383-5718(01)00257-1","article-title":"Antioxidative and antimutagenic activity of yeast cell wall mannans in vitro","volume":"497","author":"L Krizkova","year":"2001","journal-title":"Mutat Res-Gen Tox En"},{"issue":"2","key":"pone.0222775.ref037","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1091\/mbc.e13-07-0396","article-title":"Distinct roles of cell wall biogenesis in yeast morphogenesis as revealed by multivariate analysis of high-dimensional morphometric data","volume":"25","author":"H Okada","year":"2014","journal-title":"Molecular Biology of the Cell"},{"issue":"12","key":"pone.0222775.ref038","doi-asserted-by":"crossref","first-page":"E1490","DOI":"10.1073\/pnas.1410400112","article-title":"Plant-derived antifungal agent poacic acid targets beta-1,3-glucan","volume":"112","author":"JS Piotrowski","year":"2015","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"2","key":"pone.0222775.ref039","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1080\/00362178185380161","article-title":"Lysis of Growing Yeast-Form Cells of Candida-Albicans by Echinocandin\u2014a Cytological Study","volume":"19","author":"A Cassone","year":"1981","journal-title":"Sabouraudia-Journal of Medical and Veterinary Mycology"},{"key":"pone.0222775.ref040","first-page":"7","article-title":"The Cell Wall Protein Ecm33 of Candida albicans is Involved in Chronological Life Span, Morphogenesis, Cell Wall Regeneration, Stress Tolerance, and Host-Cell Interaction","author":"A Gil-Bona","year":"2016","journal-title":"Frontiers in Microbiology"},{"key":"pone.0222775.ref041","doi-asserted-by":"crossref","first-page":"3341","DOI":"10.1099\/mic.0.27320-0","article-title":"The GPI-anchored protein CaEcm33p is required for cell wall integrity, morphogenesis and virulence in Candida albicans","volume":"150","author":"R Martinez-Lopez","year":"2004","journal-title":"Microbiol-Sgm"},{"issue":"47","key":"pone.0222775.ref042","doi-asserted-by":"crossref","first-page":"12047","DOI":"10.1021\/acs.jpcb.6b09371","article-title":"Molecular Organization of Dipalmitoylphosphatidylcholine Bilayers Containing Bioactive Compounds 4-(5-Heptyl-1,3,4-thiadiazol-2-yl)Benzene-1,3-diol and 4-(5-Methyl-1,3,4-thiadiazol-2-yl) Benzene-1,3-diols","volume":"120","author":"D Kluczyk","year":"2016","journal-title":"Journal of Physical Chemistry B"},{"issue":"13","key":"pone.0222775.ref043","doi-asserted-by":"crossref","first-page":"8461","DOI":"10.1074\/jbc.M807990200","article-title":"Molecular mechanisms of yeast cell wall glucan remodeling","volume":"284","author":"R Hurtado-Guerrero","year":"2009","journal-title":"J Biol Chem"},{"issue":"20","key":"pone.0222775.ref044","doi-asserted-by":"crossref","first-page":"13401","DOI":"10.1074\/jbc.M807667200","article-title":"Cell Wall beta-(1,6)-Glucan of Saccharomyces cerevisiae STRUCTURAL CHARACTERIZATION AND IN SITU SYNTHESIS","volume":"284","author":"V Aimanianda","year":"2009","journal-title":"Journal of Biological Chemistry"},{"issue":"2","key":"pone.0222775.ref045","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1128\/MMBR.00038-05","article-title":"Cell wall assembly in Saccharomyces cerevisiae","volume":"70","author":"G Lesage","year":"2006","journal-title":"Microbiology and Molecular Biology Reviews"},{"issue":"9","key":"pone.0222775.ref046","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1038\/nrmicro3090","article-title":"How carbohydrates sculpt cells: chemical control of morphogenesis in the yeast cell wall","volume":"11","author":"ECJ Arroyo","year":"2013","journal-title":"Nature Reviews Microbiology"},{"issue":"3","key":"pone.0222775.ref047","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1111\/j.1574-6976.2002.tb00613.x","article-title":"Dynamics of cell wall structure in Saccharomyces cerevisiae","volume":"26","author":"FM Klis","year":"2002","journal-title":"Fems Microbiology Reviews"},{"issue":"8","key":"pone.0222775.ref048","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1099\/mic.0.000499","article-title":"Microbe Profile: Candida albicans: a shape-changing, opportunistic pathogenic fungus of humans.","volume":"163","author":"NAR Gow","year":"2017","journal-title":"Microbiol-Sgm"},{"issue":"10","key":"pone.0222775.ref049","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1038\/nri3897","article-title":"Immune defence against Candida fungal infections","volume":"15","author":"MG Netea","year":"2015","journal-title":"Nature Reviews Immunology"},{"issue":"1","key":"pone.0222775.ref050","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1128\/EC.5.1.140-147.2006","article-title":"Candida albicans Ecm33p is important for normal cell wall architecture and interactions with host cells","volume":"5","author":"R Martinez-Lopez","year":"2006","journal-title":"Eukaryot Cell"},{"issue":"2","key":"pone.0222775.ref051","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1038\/nrmicro.2016.157","article-title":"Candida albicans cell-type switching and functional plasticity in the mammalian host","volume":"15","author":"SM Noble","year":"2017","journal-title":"Nat Rev Microbiol"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0222775","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,23]],"date-time":"2021-01-23T15:19:24Z","timestamp":1611415164000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0222775"}},"subtitle":[],"editor":[{"given":"Megan D.","family":"Lenardon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,9,30]]},"references-count":51,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2019,9,30]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0222775","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,30]]}}}