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High speed fluoroscopic evaluation of laryngopharyngeal movement during exercise in clinically normal horses and in horses effected with dorsal displacement of the soft palate. M.Sc. Thesis, Royal Veterinary College 2013."},{"key":"pone.0224524.ref046","first-page":"577","volume-title":"Equine Surgery","author":"NG Ducharme","year":"2012"},{"key":"pone.0224524.ref047","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1164\/ajrccm\/142.2.474","article-title":"Respiratory muscle fatigue. 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