{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T21:23:30Z","timestamp":1790717010608,"version":"4.1.0"},"reference-count":33,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2020,1,7]],"date-time":"2020-01-07T00:00:00Z","timestamp":1578355200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["2014-05517"],"award-info":[{"award-number":["2014-05517"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0225481","type":"journal-article","created":{"date-parts":[[2020,1,7]],"date-time":"2020-01-07T23:05:39Z","timestamp":1578438339000},"page":"e0225481","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":65,"title":["Microvesicles from Lactobacillus reuteri (DSM-17938) completely reproduce modulation of gut motility by bacteria in mice"],"prefix":"10.1371","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6518-8137","authenticated-orcid":true,"given":"Christine L.","family":"West","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew M.","family":"Stanisz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Kang","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Champagne-Jorgensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Bienenstock","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang A.","family":"Kunze","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2020,1,7]]},"reference":[{"key":"pone.0225481.ref001","doi-asserted-by":"crossref","first-page":"1948","DOI":"10.1128\/IAI.06014-11","article-title":"Membrane vesicle release in bacteria, eukaryotes, and archaea: A conserved yet underappreciated aspect of microbial life","volume":"80","author":"BL Deatherage","year":"2012","journal-title":"Infect Immun"},{"key":"pone.0225481.ref002","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1038\/s41579-018-0112-2","article-title":"Types and origins of bacterial membrane vesicles","volume":"17","author":"M Toyofuku","year":"2019","journal-title":"Nat Rev Microbiol"},{"key":"pone.0225481.ref003","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1038\/nrmicro3480","article-title":"Through the wall: extracellular vesicles in Gram-positive bacteria, mycobacteria and fungi","volume":"13","author":"L Brown","year":"2015","journal-title":"Nat Rev Microbiol"},{"key":"pone.0225481.ref004","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/ijms18061287","article-title":"The therapeutic benefit of bacterial membrane vesicles","volume":"18","author":"NJ Bitto","year":"2017","journal-title":"Int J Mol Sci"},{"key":"pone.0225481.ref005","doi-asserted-by":"crossref","first-page":"2645","DOI":"10.1101\/gad.1299905","article-title":"Bacterial outer membrane vesicles and the host\u2013pathogen interaction","volume":"19","author":"MJ Kuehn","year":"2005","journal-title":"Genes Dev"},{"key":"pone.0225481.ref006","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1016\/0005-2736(76)90058-4","article-title":"Release of Outer Membrane Fragments from Normally Growing Escherichia coli","volume":"455","author":"D Hoekstra","year":"1976","journal-title":"Biochem Biophys Acta"},{"key":"pone.0225481.ref007","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/j.chom.2012.08.004","article-title":"Outer Membrane Vesicles of a Human Commensal Mediate Immune Regulation and Disease Protection","volume":"12","author":"Y Shen","year":"2012","journal-title":"Cell Host Microbe"},{"key":"pone.0225481.ref008","doi-asserted-by":"crossref","first-page":"5425","DOI":"10.1002\/pmic.200900338","article-title":"Gram-positive bacteria produce membrane vesicles: Proteomics-based characterization of Staphylococcus aureus-derived membrane vesicles","volume":"9","author":"EY Lee","year":"2009","journal-title":"Proteomics"},{"key":"pone.0225481.ref009","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1096\/fj.14-259721","article-title":"Gut commensal microvesicles reproduce parent bacterial signals to host immune and enteric nervous systems","volume":"29","author":"K Al-Nedawi","year":"2015","journal-title":"FASEB J"},{"key":"pone.0225481.ref010","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1186\/s12866-017-0977-7","article-title":"Lactobacillus-derived extracellular vesicles enhance host immune responses against vancomycin-resistant enterococci","volume":"17","author":"M Li","year":"2017","journal-title":"BMC Microbiol"},{"key":"pone.0225481.ref011","first-page":"1","article-title":"Isolation and characterization of Lactobacillus -derived membrane vesicles","volume":"9","author":"SN Dean","year":"2019","journal-title":"Sci Rep"},{"key":"pone.0225481.ref012","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fmicb.2017.01040","article-title":"Detection and Physicochemical Characterization of Membrane Vesicles (MVs) of Lactobacillus reuteri DSM 17938","volume":"8","author":"R Grande","year":"2017","journal-title":"Front Microbiol"},{"key":"pone.0225481.ref013","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.1007\/s00431-014-2328-0","article-title":"The efficacy of Lactobacillus reuteri DSM 17938 in infants and children: a review of the current evidence","volume":"173","author":"M Urba\u0144ska","year":"2014","journal-title":"Eur J Pediatr"},{"key":"pone.0225481.ref014","doi-asserted-by":"crossref","first-page":"6032","DOI":"10.1128\/AEM.00991-08","article-title":"Removal of antibiotic resistance gene-carrying plasmids from Lactobacillus reuteri ATCC 55730 and characterization of the resulting daughter strain, L. reuteri DSM 17938","volume":"74","author":"A Rosander","year":"2008","journal-title":"Appl Environ Microbiol"},{"key":"pone.0225481.ref015","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.jpeds.2014.09.020","article-title":"Probiotics for infantile colic: a randomized, double-blind, placebo-controlled trial investigating Lactobacillus reuteri DSM 17938","volume":"166","author":"K Chau","year":"2015","journal-title":"J Pediatr"},{"key":"pone.0225481.ref016","doi-asserted-by":"crossref","first-page":"e526","DOI":"10.1542\/peds.2010-0433","article-title":"Lactobacillus reuteri DSM 17938 in infantile colic: a randomized, double-blind, placebo-controlled trial","volume":"126","author":"F Savino","year":"2010","journal-title":"Pediatrics"},{"key":"pone.0225481.ref017","first-page":"e300","article-title":"Study Group, Vandenplas Y. Lactobacillus reuteri DSM 17938 effectively reduces the duration of acute diarrhoea in hospitalised children","volume":"103","author":"PROBAGE Dinleyici EC","year":"2014","journal-title":"Acta Paediatr"},{"key":"pone.0225481.ref018","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1016\/j.jpeds.2010.04.066","article-title":"Lactobacillus reuteri (DSM 17938) in infants with functional chronic constipation: A double-blind, randomized, placebo-controlled study","volume":"157","author":"P Coccorullo","year":"2010","journal-title":"J Pediatr"},{"key":"pone.0225481.ref019","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1111\/j.1365-2362.2010.02425.x","article-title":"Lactobacillus reuteri accelerates gastric emptying and improves regurgitation in infants","volume":"41","author":"F Indrio","year":"2011","journal-title":"Eur J Clin Invest"},{"key":"pone.0225481.ref020","doi-asserted-by":"crossref","first-page":"e205","DOI":"10.1111\/nmo.12072","article-title":"Spatiotemporal maps reveal regional differences in the effects on gut motility for Lactobacillus reuteri and rhamnosus strains","volume":"25","author":"RY Wu","year":"2013","journal-title":"Neurogastroenterol Motil"},{"key":"pone.0225481.ref021","doi-asserted-by":"crossref","first-page":"338","DOI":"10.3748\/wjg.v24.i3.338","article-title":"Effect of Lactobacillus rhamnosus GG supernatant on serotonin transporter expression in rats with post- infectious irritable bowel syndrome","volume":"24","author":"Y-N Cao","year":"2018","journal-title":"World J Gastroenterol"},{"key":"pone.0225481.ref022","first-page":"1","article-title":"Lactobacillus rhamnosus GG supernatant enhance neonatal resistance to systemic Escherichia coli K1 infection by accelerating development of intestinal defense","volume":"7","author":"X He","year":"2017","journal-title":"Sci Rep"},{"key":"pone.0225481.ref023","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1152\/ajpgi.00024.2012","article-title":"Lactobacillus rhamnosus GG culture supernatant ameliorates acute alcohol-induced intestinal permeability and liver injury","volume":"303","author":"Y Wang","year":"2012","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"key":"pone.0225481.ref024","first-page":"559","article-title":"Dual Effects of Cell Free Supernatants from Lactobacillus acidophilus and Lactobacillus rhamnosus GG in Regulation of MMP-9 by Up-Regulating TIMP-1 and Down-Regulating CD147 in PMA- Differentiated THP-1 Cells","volume":"19","author":"F Maghsood","year":"2018","journal-title":"Cell J"},{"key":"pone.0225481.ref025","doi-asserted-by":"crossref","first-page":"1537","DOI":"10.1002\/ibd.20930","article-title":"Bacteria-free solution derived from Lactobacillus plantarum inhibits multiple NF-kappaB pathways and inhibits proteasome function","volume":"15","author":"EO Petrof","year":"2009","journal-title":"Inflamm Bowel Dis"},{"key":"pone.0225481.ref026","doi-asserted-by":"crossref","first-page":"10532","DOI":"10.3748\/wjg.v22.i48.10532","article-title":"Effects of Saccharomyces cerevisiae or boulardii yeasts on acute stress induced intestinal dysmotility","volume":"22","author":"C West","year":"2016","journal-title":"World J Gastroenterol"},{"key":"pone.0225481.ref027","first-page":"1","article-title":"Lactobacillus casei BL23 Produces Microvesicles Carrying Proteins That Have Been Associated with Its Probiotic Effect","volume":"8","author":"APD Rubio","year":"2017","journal-title":"Front Microbiol"},{"key":"pone.0225481.ref028","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1159\/000322233","article-title":"Functional Analysis of the p40 and p75 Proteins from Lactobacillus casei BL23","volume":"19","author":"C B\u00e4uerl","year":"2010","journal-title":"J Mol Microbiol Biotechnol"},{"key":"pone.0225481.ref029","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1111\/j.1365-2982.2009.01384.x","article-title":"Lactobacillus reuteri ingestion and IKCa channel blockade have similar effects on rat colon motility and myenteric neurones","volume":"22","author":"B Wang","year":"2010","journal-title":"Neurogastroenterol Motil"},{"key":"pone.0225481.ref030","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fnins.2017.00588","article-title":"Antibiotic driven changes in gut motility suggest direct modulation of enteric nervous system","volume":"11","author":"T Delungahawatta","year":"2017","journal-title":"Front Neurosci"},{"key":"pone.0225481.ref031","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/j.tig.2004.05.007","article-title":"Evolution of cell-cell signaling in animals: Did late horizontal gene transfer from bacteria have a role?","author":"LM Iyer","year":"2004","journal-title":"Trends in Genetics"},{"key":"pone.0225481.ref032","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1002\/bies.201100024","article-title":"Probiotics function mechanistically as delivery vehicles for neuroactive compounds: Microbial endocrinology in the design and use of probiotics","volume":"33","author":"M. Lyte","year":"2011","journal-title":"Bioessays"},{"key":"pone.0225481.ref033","doi-asserted-by":"crossref","first-page":"2261","DOI":"10.1111\/j.1582-4934.2009.00686.x","article-title":"Lactobacillus reuteri enhances excitability of colonic AH neurons by inhibiting calcium-dependent potassium channel opening","volume":"13","author":"WA Kunze","year":"2009","journal-title":"J Cell Mol Med"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0225481","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,29]],"date-time":"2024-07-29T21:04:40Z","timestamp":1722287080000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0225481"}},"subtitle":[],"editor":[{"given":"Franck","family":"Carbonero","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,1,7]]},"references-count":33,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2020,1,7]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0225481","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,7]]}}}