{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T12:29:36Z","timestamp":1792067376552,"version":"4.4.0"},"reference-count":33,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2020,1,30]],"date-time":"2020-01-30T00:00:00Z","timestamp":1580342400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"JSPS Kakenhi","award":["JP30594296"],"award-info":[{"award-number":["JP30594296"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0228565","type":"journal-article","created":{"date-parts":[[2020,1,30]],"date-time":"2020-01-30T13:39:56Z","timestamp":1580391596000},"page":"e0228565","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":13,"title":["Usefulness of rapid on-site evaluation specimens from endoscopic ultrasound-guided fine-needle aspiration for cancer gene panel testing: A retrospective study"],"prefix":"10.1371","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5137-3868","authenticated-orcid":true,"given":"Tetsuya","family":"Ishizawa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naohiko","family":"Makino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akiko","family":"Matsuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuharu","family":"Kakizaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshikazu","family":"Kobayashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chisaki","family":"Ikeda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinpei","family":"Sugahara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michihiko","family":"Tsunoda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5623-4250","authenticated-orcid":true,"given":"Yoshiyuki","family":"Ueno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2020,1,30]]},"reference":[{"issue":"10039","key":"pone.0228565.ref001","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/S0140-6736(16)00141-0","article-title":"Pancreatic cancer","volume":"388","author":"T Kamisawa","year":"2016","journal-title":"The Lancet"},{"issue":"11","key":"pone.0228565.ref002","doi-asserted-by":"crossref","first-page":"2913","DOI":"10.1158\/0008-5472.CAN-14-0155","article-title":"Projecting cancer incidence and deaths to 2030: the unexpected burden of thyroid, liver, and pancreas cancers in the United States","volume":"74","author":"L Rahib","year":"2014","journal-title":"Cancer Res"},{"issue":"17","key":"pone.0228565.ref003","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1056\/NEJMra0901557","article-title":"Pancreatic cancer","volume":"362","author":"M. Hidalgo","year":"2010","journal-title":"N Engl J Med"},{"issue":"19","key":"pone.0228565.ref004","doi-asserted-by":"crossref","first-page":"1817","DOI":"10.1056\/NEJMoa1011923","article-title":"FOLFIRINOX versus gemcitabine for metastatic pancreatic cancer","volume":"364","author":"T Conroy","year":"2011","journal-title":"N Engl J Med"},{"issue":"18","key":"pone.0228565.ref005","doi-asserted-by":"crossref","first-page":"1691","DOI":"10.1056\/NEJMoa1304369","article-title":"Increased survival in pancreatic cancer with nab-paclitaxel plus gemcitabine","volume":"369","author":"DD Von Hoff","year":"2013","journal-title":"N Engl J Med"},{"issue":"2","key":"pone.0228565.ref006","doi-asserted-by":"crossref","DOI":"10.3390\/cancers10020035","article-title":"Reviewing the Utility of EUS FNA to Advance Precision Medicine in Pancreatic Cancer","volume":"10","author":"W Berry","year":"2018","journal-title":"Cancers (Basel)"},{"issue":"7","key":"pone.0228565.ref007","doi-asserted-by":"crossref","first-page":"4553","DOI":"10.18632\/oncotarget.2972","article-title":"Molecular landscape of pancreatic cancer: implications for current clinical trials","volume":"6","author":"GM Heestand","year":"2015","journal-title":"Oncotarget"},{"issue":"3","key":"pone.0228565.ref008","doi-asserted-by":"crossref","first-page":"e0193692","DOI":"10.1371\/journal.pone.0193692","article-title":"K-ras mutation analysis of residual liquid-based cytology specimens from endoscopic ultrasound-guided fine needle aspiration improves cell block diagnosis of pancreatic ductal adenocarcinoma","volume":"13","author":"Y Sekita-Hatakeyama","year":"2018","journal-title":"PLoS One"},{"issue":"5897","key":"pone.0228565.ref009","doi-asserted-by":"crossref","first-page":"1801","DOI":"10.1126\/science.1164368","article-title":"Core signaling pathways in human pancreatic cancers revealed by global genomic analyses","volume":"321","author":"S Jones","year":"2008","journal-title":"Science"},{"issue":"6349","key":"pone.0228565.ref010","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1126\/science.aan6733","article-title":"Mismatch repair deficiency predicts response of solid tumors to PD-1 blockade","volume":"357","author":"DT Le","year":"2017","journal-title":"Science"},{"issue":"4","key":"pone.0228565.ref011","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1056\/NEJMoa1903387","article-title":"Maintenance Olaparib for Germline BRCA-Mutated Metastatic Pancreatic Cancer","volume":"381","author":"T Golan","year":"2019","journal-title":"N Engl J Med"},{"issue":"3","key":"pone.0228565.ref012","first-page":"305","article-title":"Response to a third-line mitomycin C (MMC)-based chemotherapy in a patient with metastatic pancreatic adenocarcinoma carrying germline BRCA2 mutation","volume":"9","author":"P Chalasani","year":"2008","journal-title":"JOP"},{"issue":"1","key":"pone.0228565.ref013","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1158\/1535-7163.MCT-10-0893","article-title":"Personalizing cancer treatment in the age of global genomic analyses: PALB2 gene mutations and the response to DNA damaging agents in pancreatic cancer","volume":"10","author":"MC Villarroel","year":"2011","journal-title":"Mol Cancer Ther"},{"issue":"4","key":"pone.0228565.ref014","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1038\/s41591-019-0367-9","article-title":"Protective autophagy elicited by RAF\u2014>MEK\u2014>ERK inhibition suggests a treatment strategy for RAS-driven cancers","volume":"25","author":"CG Kinsey","year":"2019","journal-title":"Nat Med"},{"issue":"34","key":"pone.0228565.ref015","doi-asserted-by":"crossref","first-page":"54526","DOI":"10.18632\/oncotarget.9440","article-title":"Targeted next generation sequencing of endoscopic ultrasound acquired cytology from ampullary and pancreatic adenocarcinoma has the potential to aid patient stratification for optimal therapy selection","volume":"7","author":"FC Gleeson","year":"2016","journal-title":"Oncotarget"},{"issue":"42","key":"pone.0228565.ref016","doi-asserted-by":"crossref","first-page":"26900","DOI":"10.18632\/oncotarget.24809","article-title":"Liquid biopsy in pancreatic cancer: the beginning of a new era","volume":"9","author":"DK Yadav","year":"2018","journal-title":"Oncotarget"},{"issue":"9","key":"pone.0228565.ref017","doi-asserted-by":"crossref","first-page":"2263","DOI":"10.1111\/j.1572-0241.2002.05980.x","article-title":"Quantitative analysis of K-ras gene mutation in pancreatic tissue obtained by endoscopic ultrasonography-guided fine needle aspiration: clinical utility for diagnosis of pancreatic tumor","volume":"97","author":"M Tada","year":"2002","journal-title":"Am J Gastroenterol"},{"issue":"7","key":"pone.0228565.ref018","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1111\/j.1349-7006.2006.00216.x","article-title":"High sensitivity detection of epidermal growth factor receptor mutations in the pleural effusion of non-small cell lung cancer patients","volume":"97","author":"H Kimura","year":"2006","journal-title":"Cancer Sci"},{"key":"pone.0228565.ref019","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1186\/1471-2407-13-405","article-title":"Simultaneous identification of 36 mutations in KRAS codons 61 and 146, BRAF, NRAS, and PIK3CA in a single reaction by multiplex assay kit","volume":"13","author":"H Bando","year":"2013","journal-title":"BMC Cancer"},{"issue":"7616","key":"pone.0228565.ref020","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1038\/nature19057","article-title":"Analysis of protein-coding genetic variation in 60,706 humans","volume":"536","author":"M Lek","year":"2016","journal-title":"Nature"},{"issue":"12","key":"pone.0228565.ref021","doi-asserted-by":"crossref","first-page":"e1001025","DOI":"10.1371\/journal.pcbi.1001025","article-title":"Identifying a high fraction of the human genome to be under selective constraint using GERP++","volume":"6","author":"EV Davydov","year":"2010","journal-title":"PLoS Comput Biol"},{"issue":"28","key":"pone.0228565.ref022","doi-asserted-by":"crossref","first-page":"9451","DOI":"10.3748\/wjg.v20.i28.9451","article-title":"Rapid on-site evaluation of endoscopic-ultrasound-guided fine-needle aspiration diagnosis of pancreatic masses","volume":"20","author":"J Iglesias-Garcia","year":"2014","journal-title":"World J Gastroenterol"},{"issue":"5","key":"pone.0228565.ref023","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1002\/dc.24139","article-title":"Prospective comparison of cytological specimen adequacy assessment by different rapid staining techniques for rapid on-site evaluation in fine needle aspiration cytology and their cost-effectiveness","volume":"47","author":"P Agarwal","year":"2019","journal-title":"Diagn Cytopathol"},{"issue":"1","key":"pone.0228565.ref024","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1309\/AJCP28LAOOKSZSVW","article-title":"No evidence for interference of h&e staining in DNA testing: usefulness of DNA extraction from H&E-stained archival tissue sections","volume":"138","author":"T Morikawa","year":"2012","journal-title":"Am J Clin Pathol"},{"issue":"7","key":"pone.0228565.ref025","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1016\/j.gie.2006.11.014","article-title":"Can endosonographers evaluate on-site cytologic adequacy? A comparison with cytotechnologists","volume":"65","author":"AD Savoy","year":"2007","journal-title":"Gastrointest Endosc"},{"issue":"11","key":"pone.0228565.ref026","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1002\/cncy.21935","article-title":"FNA smears of pancreatic ductal adenocarcinoma are superior to formalin-fixed paraffin-embedded tissue as a source of DNA: Comparison of targeted KRAS amplification and genotyping in matched preresection and postresection samples","volume":"125","author":"CP Hartley","year":"2017","journal-title":"Cancer Cytopathol"},{"issue":"1","key":"pone.0228565.ref027","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1002\/cncy.21476","article-title":"Multiplatform comparison of molecular oncology tests performed on cytology specimens and formalin-fixed, paraffin-embedded tissue","volume":"123","author":"MP Gailey","year":"2015","journal-title":"Cancer Cytopathol"},{"issue":"6","key":"pone.0228565.ref028","doi-asserted-by":"crossref","first-page":"1961","DOI":"10.1016\/S0002-9440(10)64472-0","article-title":"Effect of Fixatives and Tissue Processing on the Content and Integrity of Nucleic Acids","volume":"161","author":"M Srinivasan","year":"2002","journal-title":"The American Journal of Pathology"},{"issue":"5","key":"pone.0228565.ref029","doi-asserted-by":"crossref","first-page":"3875","DOI":"10.3892\/ol.2016.5168","article-title":"Diagnosis of pancreatic lesions collected by endoscopic ultrasound-guided fine-needle aspiration using next-generation sequencing","volume":"12","author":"E Kameta","year":"2016","journal-title":"Oncol Lett"},{"issue":"4","key":"pone.0228565.ref030","doi-asserted-by":"crossref","first-page":"518","DOI":"10.5858\/arpa.2011-0678-OA","article-title":"Validation of whole slide imaging for primary diagnosis in surgical pathology","volume":"137","author":"TW Bauer","year":"2013","journal-title":"Arch Pathol Lab Med"},{"issue":"7","key":"pone.0228565.ref031","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1111\/his.12879","article-title":"Validation of digital pathology imaging for primary histopathological diagnosis","volume":"68","author":"DR Snead","year":"2016","journal-title":"Histopathology"},{"issue":"5","key":"pone.0228565.ref032","doi-asserted-by":"crossref","first-page":"618","DOI":"10.5858\/arpa.2012-0430-OA","article-title":"Digital slide imaging in cervicovaginal cytology: a pilot study","volume":"137","author":"AM Wright","year":"2013","journal-title":"Arch Pathol Lab Med"},{"issue":"12","key":"pone.0228565.ref033","doi-asserted-by":"crossref","first-page":"2081","DOI":"10.1136\/gutjnl-2017-315154","article-title":"EUS-guided fine needle biopsy of pancreatic masses can yield true histology","volume":"67","author":"JY Bang","year":"2018","journal-title":"Gut"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0228565","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,30]],"date-time":"2020-01-30T13:40:13Z","timestamp":1580391613000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0228565"}},"subtitle":[],"editor":[{"given":"Giancarlo","family":"Troncone","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,1,30]]},"references-count":33,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2020,1,30]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0228565","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,30]]}}}