{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T04:22:08Z","timestamp":1768969328596,"version":"3.49.0"},"reference-count":22,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2020,3,3]],"date-time":"2020-03-03T00:00:00Z","timestamp":1583193600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0229527","type":"journal-article","created":{"date-parts":[[2020,3,3]],"date-time":"2020-03-03T14:07:28Z","timestamp":1583244448000},"page":"e0229527","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":9,"title":["A simple and affordable kinetic assay of nucleic acids with SYBR Gold gel staining"],"prefix":"10.1371","volume":"15","author":[{"given":"Danielle","family":"Guillen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mika","family":"Schievelbein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kushkumar","family":"Patel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Davis","family":"Jose","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7181-6304","authenticated-orcid":true,"given":"Jonathan","family":"Ouellet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2020,3,3]]},"reference":[{"key":"pone.0229527.ref001","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.copbio.2017.02.007","article-title":"Lessons on enzyme kinetics from quantitative proteomics","volume":"46","author":"D Davidi","year":"2017","journal-title":"Curr Opin Biotechnol"},{"key":"pone.0229527.ref002","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1007\/978-1-62703-667-2_12","article-title":"The kinetics of ribozyme cleavage: a tool to analyze RNA folding as a function of catalysis","volume":"1086","author":"N. Zingler","year":"2014","journal-title":"Methods Mol Biol Clifton NJ"},{"key":"pone.0229527.ref003","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1080\/bih.76.3.127.132","article-title":"Staining nucleic acids and proteins in electrophoresis gels","volume":"76","author":"LR Williams","year":"2001","journal-title":"Biotech Histochem"},{"key":"pone.0229527.ref004","first-page":"651","article-title":"Monitoring Hammerhead Ribozyme-Catalyzed Cleavage with a Fluorescein-Labeled Substrate: Effects of Magnesium Ions and Antibiotic Inhibitors","volume":"76","author":"CS Chow","year":"1999","journal-title":"A Biochemistry Laboratory: Part 2. J Chem Educ"},{"key":"pone.0229527.ref005","doi-asserted-by":"crossref","first-page":"2940","DOI":"10.1021\/acschembio.7b00621","article-title":"Large Scale Mutational and Kinetic Analysis of a Self-Hydrolyzing Deoxyribozyme","volume":"12","author":"V Dhamodharan","year":"2017","journal-title":"ACS Chem Biol"},{"key":"pone.0229527.ref006","volume-title":"Chapter 3 Kinetic analysis of ribozyme cleavage","author":"PC Bevilacqua","year":"2003"},{"key":"pone.0229527.ref007","doi-asserted-by":"crossref","first-page":"1348","DOI":"10.1017\/S1355838299991185","article-title":"Rapid kinetic characterization of hammerhead ribozymes by real-time monitoring of fluorescence resonance energy transfer (FRET)","volume":"5","author":"KK Singh","year":"1999","journal-title":"RNA"},{"key":"pone.0229527.ref008","doi-asserted-by":"crossref","first-page":"e112","DOI":"10.1093\/nar\/gnf111","article-title":"A continuous kinetic assay for RNA\u2010cleaving deoxyribozymes, exploiting ethidium bromide as an extrinsic fluorescent probe","volume":"30","author":"D Ferrari","year":"2002","journal-title":"Nucleic Acids Res"},{"key":"pone.0229527.ref009","doi-asserted-by":"crossref","first-page":"e0135984","DOI":"10.1371\/journal.pone.0135984","article-title":"A High-Throughput Kinetic Assay for RNA-Cleaving Deoxyribozymes","volume":"10","author":"J Eriksson","year":"2015","journal-title":"PLOS ONE"},{"key":"pone.0229527.ref010","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1006\/abio.1998.3067","article-title":"Characterization of SYBR Gold Nucleic Acid Gel Stain: A Dye Optimized for Use with 300-nm Ultraviolet Transilluminators","volume":"268","author":"RS Tuma","year":"1999","journal-title":"Anal Biochem"},{"key":"pone.0229527.ref011","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/bs.pmbts.2018.07.006","article-title":"Structural Simplicity and Mechanistic Complexity in the Hammerhead Ribozyme","volume":"159","author":"SM O\u2019Rourke","year":"2018","journal-title":"Prog Mol Biol Transl Sci"},{"key":"pone.0229527.ref012","doi-asserted-by":"crossref","first-page":"9121","DOI":"10.1021\/ja403585e","article-title":"Small, highly active DNAs that hydrolyze DNA","volume":"135","author":"H Gu","year":"2013","journal-title":"J Am Chem Soc"},{"key":"pone.0229527.ref013","doi-asserted-by":"crossref","first-page":"1916","DOI":"10.1261\/rna.7159504","article-title":"Artificial tertiary motifs stabilize trans-cleaving hammerhead ribozymes under conditions of submillimolar divalent ions and high temperatures","volume":"10","author":"V Saksmerprome","year":"2004","journal-title":"RNA N Y N"},{"key":"pone.0229527.ref014","doi-asserted-by":"crossref","first-page":"8783","DOI":"10.1093\/nar\/15.21.8783","article-title":"Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates","volume":"15","author":"JF Milligan","year":"1987","journal-title":"Nucleic Acids Res"},{"key":"pone.0229527.ref015","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/0003-2697(66)90012-1","article-title":"A new fluorometric method for RNA and DNA determination","volume":"17","author":"JB Le Pecq","year":"1966","journal-title":"Anal Biochem"},{"key":"pone.0229527.ref016","doi-asserted-by":"crossref","first-page":"5869","DOI":"10.1093\/nar\/14.14.5869","article-title":"Synthesis and properties of defined DNA oligomers containing base mispairs involving 2-aminopurine","volume":"14","author":"R Eritja","year":"1986","journal-title":"Nucleic Acids Res"},{"key":"pone.0229527.ref017","doi-asserted-by":"crossref","first-page":"4231","DOI":"10.1073\/pnas.0900803106","article-title":"Spectroscopic studies of position-specific DNA \u201cbreathing\u201d fluctuations at replication forks and primer-template junctions","volume":"106","author":"D Jose","year":"2009","journal-title":"Proc Natl Acad Sci"},{"key":"pone.0229527.ref018","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1016\/j.tibs.2016.04.010","article-title":"Catalytic DNA: Scope, Applications, and Biochemistry of Deoxyribozymes","volume":"41","author":"SK Silverman","year":"2016","journal-title":"Trends Biochem Sci"},{"key":"pone.0229527.ref019","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1021\/ed062p924","article-title":"Zinc enzymes","volume":"62","author":"I Bertini","year":"1985","journal-title":"J Chem Educ"},{"key":"pone.0229527.ref020","first-page":"85","article-title":"Luchinat C. The Reaction Pathways of Zinc Enzymes and Related Biological Catalysts. Bioinorganic Chemistry. Mill Valley","author":"Bertini Ivan","year":"1994","journal-title":"CA: University Science Books"},{"key":"pone.0229527.ref021","doi-asserted-by":"crossref","first-page":"1437S","DOI":"10.1093\/jn\/130.5.1437S","article-title":"Function and Mechanism of Zinc Metalloenzymes","volume":"130","author":"KA McCall","year":"2000","journal-title":"J Nutr"},{"key":"pone.0229527.ref022","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S0277-5387(00)81239-5","article-title":"The biochemistry of zinc","volume":"6","author":"RJP Williams","year":"1987","journal-title":"Polyhedron"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0229527","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,3,3]],"date-time":"2020-03-03T14:07:41Z","timestamp":1583244461000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0229527"}},"subtitle":[],"editor":[{"given":"Ruijie","family":"Deng","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2020,3,3]]},"references-count":22,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2020,3,3]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0229527","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3,3]]}}}