{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T01:22:49Z","timestamp":1788312169381,"version":"build-2803163510"},"reference-count":71,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2020,3,16]],"date-time":"2020-03-16T00:00:00Z","timestamp":1584316800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81672048"],"award-info":[{"award-number":["81672048"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31572240"],"award-info":[{"award-number":["31572240"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31872959"],"award-info":[{"award-number":["31872959"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31802184"],"award-info":[{"award-number":["31802184"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0230381","type":"journal-article","created":{"date-parts":[[2020,3,16]],"date-time":"2020-03-16T17:27:25Z","timestamp":1584379645000},"page":"e0230381","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":21,"title":["The immunogenicity and protective immunity of multi-epitopes\u00a0DNA prime-protein\u00a0\u00a0boost vaccines encoding Amastin-Kmp-11, Kmp11-Gp63 and Amastin-Gp63 against visceral leishmaniasis"],"prefix":"10.1371","volume":"15","author":[{"given":"Jianhui","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinlei","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiwan","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiwei","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dali","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianping","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2020,3,16]]},"reference":[{"key":"pone.0230381.ref001","unstructured":"World Health Organization. Leishmaniasis. 2019 March 14 [cited 19 August 2019]. In WHO [Internet]. Available from: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/leishmaniasis"},{"key":"pone.0230381.ref002","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jim.2015.03.017","article-title":"Vaccines for visceral leishmaniasis: A review","volume":"422","author":"K Jain","year":"2015","journal-title":"J. Immunol. Methods"},{"issue":"2","key":"pone.0230381.ref003","first-page":"163","article-title":"Recent advances in antileishmanial drug development","volume":"6","author":"AJ Davis","year":"2005","journal-title":"Curr Opin Investig Drugs"},{"issue":"5","key":"pone.0230381.ref004","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1097\/00001432-200310000-00005","article-title":"Current treatment approaches to leishmaniasis","volume":"16","author":"J. Berman","year":"2003","journal-title":"Curr. Opin. Infect. Dis"},{"issue":"3","key":"pone.0230381.ref005","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.trsl.2013.03.006","article-title":"New vaccines for neglected parasitic diseases and dengue","volume":"162","author":"CM Beaumier","year":"2013","journal-title":"Transl. Res"},{"issue":"Suppl 2","key":"pone.0230381.ref006","doi-asserted-by":"crossref","first-page":"B244","DOI":"10.1016\/j.vaccine.2012.11.080","article-title":"Case study for a vaccine against leishmaniasis","volume":"31","author":"J Alvar","year":"2013","journal-title":"Vaccine"},{"issue":"10","key":"pone.0230381.ref007","doi-asserted-by":"crossref","first-page":"e1874","DOI":"10.1371\/journal.pntd.0001874","article-title":"Cytokine responses to novel antigens in an Indian population living in an area endemic for visceral leishmaniasis","volume":"6","author":"OP Singh","year":"2012","journal-title":"PLoS Negl. Trop. Dis"},{"issue":"10","key":"pone.0230381.ref008","doi-asserted-by":"crossref","first-page":"e1004419","DOI":"10.1371\/journal.ppat.1004419","article-title":"Leishmania donovani infection causes distinct epigenetic DNA methylation changes in host macrophages","volume":"10","author":"AK Marr","year":"2014","journal-title":"PLoS Pathog"},{"issue":"2","key":"pone.0230381.ref009","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1038\/sj.icb7100011","article-title":"Balancing immunity and pathology in visceral leishmaniasis","volume":"85","author":"AC Stanley","year":"2007","journal-title":"Immunol. Cell Biol"},{"key":"pone.0230381.ref010","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1186\/s13071-016-1412-x","article-title":"Regulation of immunity during visceral Leishmania infection","volume":"9","author":"V Rodrigues","year":"2016","journal-title":"Parasites & vectors"},{"issue":"4","key":"pone.0230381.ref011","doi-asserted-by":"crossref","first-page":"1773","DOI":"10.1128\/IAI.68.4.1773-1780.2000","article-title":"Interleukin-4 receptor alpha-deficient BALB\/c mice show an unimpaired T helper 2 polarization in response to Leishmania major infection","volume":"68","author":"M Mohrs","year":"2000","journal-title":"Infect. Immun"},{"issue":"8","key":"pone.0230381.ref012","doi-asserted-by":"crossref","first-page":"4804","DOI":"10.1128\/IAI.71.8.4804-4807.2003","article-title":"Both interleukin-4 (IL-4) and IL-4 receptor alpha signaling contribute to the development of hepatic granulomas with optimal antileishmanial activity","volume":"71","author":"S Stager","year":"2003","journal-title":"Infect. Immun"},{"issue":"10","key":"pone.0230381.ref013","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1038\/nm933","article-title":"Natural antibodies and complement are endogenous adjuvants for vaccine-induced CD8+ T-cell responses","volume":"9","author":"S Stager","year":"2003","journal-title":"Nat. Med"},{"issue":"12","key":"pone.0230381.ref014","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1038\/ni861","article-title":"Defective CCR7 expression on dendritic cells contributes to the development of visceral leishmaniasis","volume":"3","author":"M Ato","year":"2002","journal-title":"Nat. Immunol"},{"issue":"10","key":"pone.0230381.ref015","doi-asserted-by":"crossref","first-page":"2848","DOI":"10.1002\/1521-4141(2001010)31:10<2848::AID-IMMU2848>3.0.CO;2-T","article-title":"IL-10 mediates susceptibility to Leishmania donovani infection","volume":"31","author":"ML Murphy","year":"2001","journal-title":"Eur. J. Immunol"},{"issue":"Suppl","key":"pone.0230381.ref016","doi-asserted-by":"crossref","first-page":"S87","DOI":"10.1017\/S0031182006001831","article-title":"Leishmania vaccines: progress and problems","volume":"133","author":"L Kedzierski","year":"2006","journal-title":"Parasitology"},{"issue":"7","key":"pone.0230381.ref017","doi-asserted-by":"crossref","first-page":"901","DOI":"10.2217\/fmb.14.43","article-title":"Immunity to visceral leishmaniasis: implications for immunotherapy","volume":"9","author":"F Khadem","year":"2014","journal-title":"Future Microbiol"},{"issue":"5","key":"pone.0230381.ref018","doi-asserted-by":"crossref","first-page":"1562","DOI":"10.1084\/jem.157.5.1562","article-title":"Surface antigens of Leishmania donovani promastigotes","volume":"157","author":"DA Lepay","year":"1983","journal-title":"J. Exp. Med"},{"issue":"3","key":"pone.0230381.ref019","doi-asserted-by":"crossref","first-page":"902","DOI":"10.1084\/jem.162.3.902","article-title":"A common major surface antigen on amastigotes and promastigotes of Leishmania species","volume":"162","author":"V Colomer-Gould","year":"1985","journal-title":"J. Exp. Med"},{"issue":"32","key":"pone.0230381.ref020","doi-asserted-by":"crossref","first-page":"6107","DOI":"10.1016\/j.vaccine.2007.05.009","article-title":"The role of CpG ODN in enhancement of immune response and protection in BALB\/c mice immunized with recombinant major surface glycoprotein of Leishmania (rgp63) encapsulated in cationic liposome","volume":"25","author":"MR Jaafari","year":"2007","journal-title":"Vaccine"},{"issue":"1","key":"pone.0230381.ref021","first-page":"1","article-title":"Protection against Leishmania major infection in genetically susceptible BALB\/c mice by gp63 delivered orally in attenuated Salmonella typhimurium (AroA- AroD-)","volume":"85","author":"D Xu","year":"1995","journal-title":"Immunology"},{"issue":"2","key":"pone.0230381.ref022","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1111\/j.1365-3024.2010.01253.x","article-title":"Cocktail of gp63 and Hsp70 induces protection against Leishmania donovani in BALB\/c mice","volume":"33","author":"T Kaur","year":"2011","journal-title":"Parasite Immunol"},{"issue":"31","key":"pone.0230381.ref023","doi-asserted-by":"crossref","first-page":"5053","DOI":"10.1016\/j.vaccine.2011.04.089","article-title":"Cellular vaccination with bone marrow-derived dendritic cells pulsed with a peptide of Leishmania infantum KMP-11 and CpG oligonucleotides induces protection in a murine model of visceral leishmaniasis","volume":"29","author":"M Agallou","year":"2011","journal-title":"Vaccine"},{"issue":"11","key":"pone.0230381.ref024","doi-asserted-by":"crossref","first-page":"7160","DOI":"10.4049\/jimmunol.174.11.7160","article-title":"Kinetoplastid membrane protein-11 DNA vaccination induces complete protection against both pentavalent antimonial-sensitive and -resistant strains of Leishmania donovani that correlates with inducible nitric oxide synthase activity and IL-4 generation: evidence for mixed Th1- and Th2-like responses in visceral leishmaniasis","volume":"174","author":"R Basu","year":"2005","journal-title":"J. Immunol"},{"issue":"12","key":"pone.0230381.ref025","doi-asserted-by":"crossref","first-page":"e1005296","DOI":"10.1371\/journal.ppat.1005296","article-title":"Amastin Knockdown in Leishmania braziliensis Affects Parasite-Macrophage Interaction and Results in Impaired Viability of Intracellular Amastigotes","volume":"11","author":"RM de Paiva","year":"2015","journal-title":"PLoS Pathog"},{"issue":"14","key":"pone.0230381.ref026","doi-asserted-by":"crossref","first-page":"2602","DOI":"10.1016\/j.vaccine.2005.12.012","article-title":"From genome to vaccines for leishmaniasis: screening 100 novel vaccine candidates against murine Leishmania major infection","volume":"24","author":"CB Stober","year":"2006","journal-title":"Vaccine"},{"issue":"10","key":"pone.0230381.ref027","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1128\/CVI.00188-06","article-title":"Amastin peptide-binding antibodies as biomarkers of active human visceral leishmaniasis","volume":"13","author":"S Rafati","year":"2006","journal-title":"Clin. Vaccine Immunol"},{"issue":"5","key":"pone.0230381.ref028","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1111\/pim.12315","article-title":"DNA vaccine against visceral leishmaniasis: a promising approach for prevention and control","volume":"38","author":"A Kumar","year":"2016","journal-title":"Parasite Immunol"},{"issue":"3","key":"pone.0230381.ref029","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/j.coi.2009.05.016","article-title":"Heterologous prime-boost vaccination","volume":"21","author":"S. Lu","year":"2009","journal-title":"Curr. Opin. Immunol"},{"issue":"10","key":"pone.0230381.ref030","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1089\/104454999314917","article-title":"DNA priming-protein boosting enhances both antigen-specific antibody and Th1-type cellular immune responses in a murine herpes simplex virus-2 gD vaccine model","volume":"18","author":"JI Sin","year":"1999","journal-title":"DNA Cell Biol"},{"issue":"31","key":"pone.0230381.ref031","doi-asserted-by":"crossref","first-page":"4555","DOI":"10.1016\/S0264-410X(03)00499-7","article-title":"Efficient induction of T helper 1 CD4+ T-cell responses to hepatitis C virus core and E2 by a DNA prime-adenovirus boost","volume":"21","author":"SH Park","year":"2003","journal-title":"Vaccine"},{"issue":"3","key":"pone.0230381.ref032","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.3892\/or.2013.2941","article-title":"Identification of HLA-A2 or HLA-A24-restricted CTL epitopes for potential HSP105-targeted immunotherapy in colorectal cancer","volume":"31","author":"Y Sawada","year":"2014","journal-title":"Oncol. Rep"},{"issue":"24","key":"pone.0230381.ref033","doi-asserted-by":"crossref","first-page":"13281","DOI":"10.1128\/JVI.01841-12","article-title":"Cross-allele cytotoxic T lymphocyte responses against 2009 pandemic H1N1 influenza A virus among HLA-A24 and HLA-A3 supertype-positive individuals","volume":"86","author":"J Liu","year":"2012","journal-title":"J. Virol"},{"issue":"28","key":"pone.0230381.ref034","doi-asserted-by":"crossref","first-page":"3249","DOI":"10.2174\/138161209789105171","article-title":"HLA-DR: molecular insights and vaccine design","volume":"15","author":"LJ Stern","year":"2009","journal-title":"Curr. Pharm. Des"},{"issue":"7","key":"pone.0230381.ref035","doi-asserted-by":"crossref","first-page":"1557","DOI":"10.1016\/j.cell.2012.11.025","article-title":"Crystal structure of the HLA-DM-HLA-DR1 complex defines mechanisms for rapid peptide selection","volume":"151","author":"W Pos","year":"2012","journal-title":"Cell"},{"issue":"14","key":"pone.0230381.ref036","doi-asserted-by":"crossref","first-page":"7608","DOI":"10.1128\/JVI.02834-06","article-title":"Direct ex vivo analyses of HLA-DR1 transgenic mice reveal an exceptionally broad pattern of immunodominance in the primary HLA-DR1-restricted CD4 T-cell response to influenza virus hemagglutinin","volume":"81","author":"KA Richards","year":"2007","journal-title":"J. Virol"},{"issue":"9","key":"pone.0230381.ref037","doi-asserted-by":"crossref","first-page":"e1295","DOI":"10.1371\/journal.pntd.0001295","article-title":"In silico analysis of six known Leishmania major antigens and in vitro evaluation of specific epitopes eliciting HLA-A2 restricted CD8 T cell response","volume":"5","author":"N Seyed","year":"2011","journal-title":"PLoS Negl. Trop. Dis"},{"issue":"9","key":"pone.0230381.ref038","doi-asserted-by":"crossref","first-page":"e24626","DOI":"10.1371\/journal.pone.0024626","article-title":"Cross-protective peptide vaccine against influenza A viruses developed in HLA-A*2402 human immunity model","volume":"6","author":"T Ichihashi","year":"2011","journal-title":"PLoS One"},{"issue":"2","key":"pone.0230381.ref039","first-page":"273","article-title":"Immunogenicity of HLA-DR1 Restricted Peptides Derived from Leishmania major gp63 Using FVB\/N-DR1 Transgenic Mouse Model","volume":"8","author":"H. Rezvan","year":"2013","journal-title":"Iranian journal of parasitology"},{"issue":"8","key":"pone.0230381.ref040","doi-asserted-by":"crossref","first-page":"4565","DOI":"10.4049\/jimmunol.0803893","article-title":"A single-chain H-2Db molecule presenting an influenza virus nucleoprotein epitope shows enhanced ability at stimulating CD8+ T cell responses in vivo","volume":"182","author":"MJ Palmowski","year":"2009","journal-title":"J. Immunol"},{"issue":"1","key":"pone.0230381.ref041","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1111\/imm.13007","article-title":"DNA prime-protein boost vaccine encoding HLA-A2, HLA-A24 and HLA-DR1 restricted epitopes of CaNA2 against visceral leishmaniasis","volume":"156","author":"J He","year":"2019","journal-title":"Immunology"},{"issue":"1","key":"pone.0230381.ref042","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1007\/s12026-015-8746-x","article-title":"Construction of recombinant Mip-FlaA dominant epitope vaccine against Legionella pneumophila and evaluation of the immunogenicity and protective immunity","volume":"64","author":"J He","year":"2016","journal-title":"Immunol. Res"},{"issue":"10","key":"pone.0230381.ref043","doi-asserted-by":"crossref","first-page":"4061","DOI":"10.1007\/s00436-016-5179-y","article-title":"Molecular detection of infection homogeneity and impact of miltefosine treatment in a Syrian golden hamster model of Leishmania donovani and L. infantum visceral leishmaniasis","volume":"115","author":"E Eberhardt","year":"2016","journal-title":"Parasitol. Res"},{"issue":"Pt 3","key":"pone.0230381.ref044","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1017\/S0031182006001600","article-title":"Specific cpb copies within the Leishmania donovani complex: evolutionary interpretations and potential clinical implications in humans","volume":"134","author":"M Hide","year":"2007","journal-title":"Parasitology"},{"key":"pone.0230381.ref045","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.vetpar.2018.03.010","article-title":"Therapeutic vaccine of killed Leishmania amazonensis plus saponin reduced parasite burden in dogs naturally infected with Leishmania infantum","volume":"254","author":"KF Viana","year":"2018","journal-title":"Vet Parasitol"},{"issue":"4","key":"pone.0230381.ref046","doi-asserted-by":"crossref","first-page":"1361","DOI":"10.1007\/s00436-017-5415-0","article-title":"Biochemical properties and vaccine effect of recombinant TPx-3 from Schistosoma japonicum","volume":"116","author":"Y Han","year":"2017","journal-title":"Parasitol Res"},{"key":"pone.0230381.ref047","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.vetpar.2018.02.027","article-title":"Protective immunity against Eimeria maxima induced by vaccines of Em14-3-3 antigen","volume":"253","author":"T Liu","year":"2018","journal-title":"Vet Parasitol"},{"issue":"8","key":"pone.0230381.ref048","doi-asserted-by":"crossref","first-page":"775","DOI":"10.4161\/21505594.2014.960726","article-title":"What is a pathogen? Toward a process view of host-parasite interactions","volume":"5","author":"PO Methot","year":"2014","journal-title":"Virulence"},{"issue":"5","key":"pone.0230381.ref049","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/j.biotechadv.2017.05.002","article-title":"Harnessing self-assembled peptide nanoparticles in epitope vaccine design","volume":"35","author":"M Negahdaripour","year":"2017","journal-title":"Biotechnol. Adv"},{"issue":"5","key":"pone.0230381.ref050","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.pt.2005.03.006","article-title":"Second-generation vaccines against leishmaniasis","volume":"21","author":"RN Coler","year":"2005","journal-title":"Trends Parasitol"},{"issue":"1","key":"pone.0230381.ref051","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.ymeth.2013.07.024","article-title":"Structure of allergens and structure based epitope predictions","volume":"66","author":"F Dall'antonia","year":"2014","journal-title":"Methods"},{"key":"pone.0230381.ref052","doi-asserted-by":"crossref","first-page":"9363750","DOI":"10.1155\/2017\/9363750","article-title":"Computer-Aided Design of an Epitope-Based Vaccine against Epstein-Barr Virus","volume":"2017","author":"J Alonso-Padilla","year":"2017","journal-title":"J Immunol Res"},{"issue":"8","key":"pone.0230381.ref053","doi-asserted-by":"crossref","first-page":"2216","DOI":"10.1021\/acschembio.7b00469","article-title":"Membrane Association Dictates Ligand Specificity for the Innate Immune Receptor NOD2","volume":"12","author":"AK Schaefer","year":"2017","journal-title":"ACS Chem. Biol"},{"key":"pone.0230381.ref054","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1007\/978-1-4939-2486-8_12","article-title":"Protein binding site analysis for drug discovery using a computational fragment-based method","volume":"1289","author":"JL Ludington","year":"2015","journal-title":"Methods Mol. Biol"},{"issue":"6","key":"pone.0230381.ref055","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1586\/14760584.2015.1029919","article-title":"Efficient vaccine against pandemic influenza: combining DNA vaccination and targeted delivery to MHC class II molecules","volume":"14","author":"G Grodeland","year":"2015","journal-title":"Expert Rev Vaccines"},{"issue":"2","key":"pone.0230381.ref056","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1038\/nri.2015.10","article-title":"The multifaceted role of CD4(+) T cells in CD8(+) T cell memory","volume":"16","author":"BJ Laidlaw","year":"2016","journal-title":"Nat. Rev. Immunol"},{"issue":"9","key":"pone.0230381.ref057","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1128\/CVI.00251-16","article-title":"Optimizing Immunization Strategies for the Induction of Antigen-Specific CD4 and CD8 T Cell Responses for Protection against Intracellular Parasites","volume":"23","author":"KA Hofmeyer","year":"2016","journal-title":"Clin. Vaccine Immunol"},{"issue":"4","key":"pone.0230381.ref058","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1111\/j.1365-2567.2010.03255.x","article-title":"T-cell exhaustion: characteristics, causes and conversion","volume":"129","author":"JS Yi","year":"2010","journal-title":"Immunology"},{"issue":"7","key":"pone.0230381.ref059","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1038\/77513","article-title":"Identification of a previously unknown antigen-specific regulatory T cell and its mechanism of suppression","volume":"6","author":"ZX Zhang","year":"2000","journal-title":"Nat. Med"},{"issue":"4","key":"pone.0230381.ref060","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1016\/j.autrev.2018.02.001","article-title":"TCRalphabeta(+)CD3(+)CD4(-)CD8(-) (double negative) T cells in autoimmunity","volume":"17","author":"D Brandt","year":"2018","journal-title":"Autoimmunity reviews"},{"issue":"9","key":"pone.0230381.ref061","doi-asserted-by":"crossref","first-page":"e1004396","DOI":"10.1371\/journal.ppat.1004396","article-title":"MHC class II restricted innate-like double negative T cells contribute to optimal primary and secondary immunity to Leishmania major","volume":"10","author":"Z Mou","year":"2014","journal-title":"PLoS Pathog"},{"key":"pone.0230381.ref062","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1111\/j.0105-2896.2004.00188.x","article-title":"The immunopathology of experimental visceral leishmaniasis","volume":"201","author":"PM Kaye","year":"2004","journal-title":"Immunol. Rev"},{"issue":"11","key":"pone.0230381.ref063","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1016\/j.pt.2004.08.009","article-title":"Macrophages, pathology and parasite persistence in experimental visceral leishmaniasis","volume":"20","author":"CR Engwerda","year":"2004","journal-title":"Trends Parasitol"},{"issue":"7","key":"pone.0230381.ref064","first-page":"e2914","article-title":"Immune regulation during chronic visceral leishmaniasis. PLoS Negl. Trop. Dis","volume":"8","author":"RJ Faleiro","year":"2014"},{"issue":"2","key":"pone.0230381.ref065","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1007\/s00281-015-0548-7","article-title":"Lessons from other diseases: granulomatous inflammation in leishmaniasis","volume":"38","author":"PM Kaye","year":"2016","journal-title":"Semin. Immunopathol"},{"issue":"4","key":"pone.0230381.ref066","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1093\/infdis\/jiy552","article-title":"The Potent ITK\/BTK Inhibitor Ibrutinib Is Effective for the Treatment of Experimental Visceral Leishmaniasis Caused by Leishmania donovani","volume":"219","author":"S Varikuti","year":"2019","journal-title":"J. Infect. Dis"},{"issue":"6","key":"pone.0230381.ref067","doi-asserted-by":"crossref","first-page":"618","DOI":"10.4269\/ajtmh.2004.70.618","article-title":"Organ-specific immunity in canine visceral leishmaniasis: analysis of symptomatic and asymptomatic dogs naturally infected with Leishmania chagasi","volume":"70","author":"MA Sanchez","year":"2004","journal-title":"Am. J. Trop. Med. Hyg"},{"issue":"6","key":"pone.0230381.ref068","doi-asserted-by":"crossref","first-page":"e2853","DOI":"10.1371\/journal.pntd.0002853","article-title":"Clinical and parasitological protection in a Leishmania infantum-macaque model vaccinated with adenovirus and the recombinant A2 antigen","volume":"8","author":"G Grimaldi","year":"2014","journal-title":"PLoS Negl. Trop. Dis"},{"key":"pone.0230381.ref069","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1186\/s13071-016-1413-9","article-title":"Study of Leishmania pathogenesis in mice: experimental considerations","volume":"9","author":"C Loeuillet","year":"2016","journal-title":"Parasites & vectors"},{"key":"pone.0230381.ref070","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1186\/s13071-015-1246-y","article-title":"HisAK70: progress towards a vaccine against different forms of leishmaniosis","volume":"8","author":"G Dominguez-Bernal","year":"2015","journal-title":"Parasites & vectors"},{"issue":"202","key":"pone.0230381.ref071","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1126\/scitranslmed.3006406","article-title":"Vaccination with leishmania hemoglobin receptor-encoding DNA protects against visceral leishmaniasis","volume":"5","author":"R Guha","year":"2013","journal-title":"Sci. Transl. Med"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0230381","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,3,16]],"date-time":"2020-03-16T17:28:02Z","timestamp":1584379682000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0230381"}},"subtitle":[],"editor":[{"given":"Paulo Lee","family":"Ho","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,3,16]]},"references-count":71,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2020,3,16]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0230381","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3,16]]}}}