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paintings","volume":"55","author":"L Beck","year":"2013","journal-title":"Radiocarbon"},{"key":"pone.0230496.ref021","doi-asserted-by":"crossref","first-page":"8769","DOI":"10.1039\/c3cs60144g","article-title":"Portable nucleic acid thermocyclers","volume":"42","author":"DR Almassian","year":"2013","journal-title":"Chem Soc Rev"},{"key":"pone.0230496.ref022","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.forsciint.2018.02.002","article-title":"Field-based detection of biological samples for forensic analysis: Established techniques, novel tools, and future innovations","volume":"285","author":"J Morrison","year":"2018","journal-title":"Forensic Sci Int"},{"key":"pone.0230496.ref023","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1073\/pnas.0610257104","article-title":"Freshly excavated fossil bones are best for amplification of ancient DNA","volume":"104","author":"M Pruvost","year":"2007","journal-title":"Proc Natl Acad Sci 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B\u00e9gou\u00ebn","year":"2014"},{"key":"pone.0230496.ref029","unstructured":"B\u00e9gou\u00ebn L. Notre campagne de fouilles dans la caverne des Trois-Fr\u00e8res (Ari\u00e8ge) en 1930\u20131931. In: XVe congr\u00e8s international d\u2019anthropologie et d\u2019arch\u00e9ologie pr\u00e9historique, Ve session de l\u2019Institut international d\u2019anthropologie, Paris, 1931."},{"key":"pone.0230496.ref030","author":"R B\u00e9gou\u00ebn","year":"2019"},{"key":"pone.0230496.ref031","unstructured":"Leroi-Gourhan Arl. Les pollens dans la caverne d\u2019Enl\u00e8ne. In: Congr\u00e8s Pr\u00e9historique de France, XXIe session, Quercy-Montauban-Cahors; 1979. vol 1: pp. 58\u201359,"},{"key":"pone.0230496.ref032","author":"H. Breuil","year":"1952"},{"key":"pone.0230496.ref033","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1002\/ajpa.23719","article-title":"Neandertal features of the deciduous and permanent teeth from Portel-Ouest Cave (Ari\u00e8ge, France)","volume":"168","author":"G Becam","year":"2019","journal-title":"Am J Phys Anthropol"},{"key":"pone.0230496.ref034","first-page":"225","article-title":"Les fouilles \u00e0 l\u2019entr\u00e9e du Portel Ouest (Loubens, Ari\u00e8ge): stratigraphie g\u00e9n\u00e9rale et passage du pal\u00e9olithique moyen au pal\u00e9olithique sup\u00e9rieur","volume":"44","author":"J. V\u00e9zian","year":"1989","journal-title":"Bull Soc Pr\u00e9historique Ari\u00e8ge"},{"key":"pone.0230496.ref035","unstructured":"Ajaja O. Datation de quelques sites moust\u00e9riens de Catalogne et du Languedoc par la m\u00e9thode U-Th. Comparaisons avec la m\u00e9thode ESR. Thesis, Institut de Pal\u00e9ontologie humaine, Paris, France. 1994."},{"key":"pone.0230496.ref036","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1006\/jasc.1999.0366","article-title":"Middle Palaeolithic subsistence in the West Cave of \"Le Portel\" (Pyr\u00e9n\u00e9es, France)","volume":"26","author":"A. Gardeisen","year":"1999","journal-title":"J Archaeol Sci"},{"key":"pone.0230496.ref037","unstructured":"Testu A. \u00c9tude pal\u00e9ontologique et biostratigraphique des Felidae et Hyaenidae pl\u00e9istoc\u00e8nes de l\u2019Europe m\u00e9diterran\u00e9enne. Thesis, Universit\u00e9 de Perpignan, Perpignan, France. 2006."},{"key":"pone.0230496.ref038","unstructured":"Prince G. Contribution \u00e0 l'\u00e9tude des industries de la grotte du Portel-Ouest (commune de Loubens, Ari\u00e8ge, France): \u00e9tude techno-typologique des industries moust\u00e9riennes. Thesis, Universit\u00e9 de Perpignan, Perpignan, France. 2000."},{"key":"pone.0230496.ref039","doi-asserted-by":"crossref","first-page":"303","DOI":"10.3406\/quate.1998.1612","article-title":"Le Portel-Ouest: associations fauniques et pal\u00e9oenvironnements sur la frange septentrionale des Pyr\u00e9n\u00e9es ari\u00e8geoises au W\u00fcrm ancien","volume":"9","author":"JL Marquet","year":"1998","journal-title":"Quaternaire"},{"key":"pone.0230496.ref040","unstructured":"V\u00e9zian R. \u00c9tude pal\u00e9ontologique des Bovinae et des Equidae de la grotte moust\u00e9rienne du Portel-Ouest (Ari\u00e8ge, France). Cadres biostatigraphique, biochronologique et pal\u00e9o-environnemental. Thesis, Universit\u00e9 de Perpignan Via Domitia. Perpignan, France. 2014. Available from http:\/\/www.theses.fr\/2014PERP1202\/document"},{"key":"pone.0230496.ref041","doi-asserted-by":"crossref","first-page":"185","DOI":"10.3406\/quate.1998.1602","article-title":"Palynologie des grottes de Montmaurin (Haute-Garonne) et du versant nord pyr\u00e9n\u00e9en. Corr\u00e9lations inters\u00e9quentielles du Pl\u00e9istoc\u00e8ne moyen \u00e0 l\u2019Holoc\u00e8ne","volume":"9","author":"J Renault-Miskovsky","year":"1998","journal-title":"Quaternaire"},{"key":"pone.0230496.ref042","first-page":"40","volume-title":"Congr\u00e8s arch\u00e9ologique de France, XXXVIIIe session, Angers","author":"D. Farge","year":"1872"},{"key":"pone.0230496.ref043","doi-asserted-by":"crossref","first-page":"13","DOI":"10.3406\/quate.1984.1473","article-title":"L\u2019apport de deux sites angevins \u00e0 la chronologie des terrasses fluviales: Roc-en-Pail en Chalonnes sur Loire et Port-Launay sur la Sarthe","volume":"21","author":"M. Gruet","year":"1984","journal-title":"Bulletin de l\u2019Association fran\u00e7aise pour l\u2019\u00e9tude du Quaternaire"},{"key":"pone.0230496.ref044","unstructured":"Roucoules R. Contribution \u00e0 l\u2019\u00e9tude du Quaternaire dans le Maine-et-Loire. L\u2019abri-sous-roche de Roc-en-Pail. Thesis, Facult\u00e9 des sciences, Paris, France. 1967."},{"key":"pone.0230496.ref045","first-page":"87","article-title":"\u00c9tude palynologique de l\u2019abri-sous-roche de Roc-en-Pail en Chalonnes-sur-Loire (Maine et Loire)","volume":"4","author":"L. 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