{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T19:11:49Z","timestamp":1792177909275,"version":"4.4.0"},"reference-count":85,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2020,4,24]],"date-time":"2020-04-24T00:00:00Z","timestamp":1587686400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0231902","type":"journal-article","created":{"date-parts":[[2020,4,24]],"date-time":"2020-04-24T17:38:00Z","timestamp":1587749880000},"page":"e0231902","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":14,"title":["Phylogenetic relationships of Pseudo-nitzschia subpacifica\u00a0(Bacillariophyceae) from the Mexican Pacific, and its production of domoic acid in culture"],"prefix":"10.1371","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1499-8337","authenticated-orcid":true,"given":"Sonia Isabel","family":"Quijano-Scheggia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aramis","family":"Olivos-Ortiz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernesto","family":"Garcia-Mendoza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaireb","family":"S\u00e1nchez-Bravo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramon","family":"Sosa-Avalos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalli","family":"Salas Marias","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong Chang","family":"Lim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2020,4,24]]},"reference":[{"issue":"2","key":"pone.0231902.ref001","doi-asserted-by":"crossref","first-page":"273","DOI":"10.5670\/oceanog.2016.32","article-title":"Biological impacts of the 2013\u20132015 warm-water anomaly in the Northeast Pacific: Winners, losers, and the future.","volume":"29","author":"LM Cavole","year":"2016","journal-title":"Oceanography"},{"issue":"9","key":"pone.0231902.ref002","doi-asserted-by":"crossref","first-page":"3414","DOI":"10.1002\/2015GL063306","article-title":"Causes and impacts of the 2014 warm anomaly in the NE Pacific","volume":"42","author":"NA Bond","year":"2015","journal-title":"Geophysical Research Letters"},{"issue":"22","key":"pone.0231902.ref003","doi-asserted-by":"crossref","first-page":"9999","DOI":"10.1002\/2015GL066628","article-title":"A tale of two California droughts: Lessons amidst record warmth and dryness in a region of complex physical and human geography","volume":"42","author":"DL Swain","year":"2015","journal-title":"Geophysical Research Letters"},{"key":"pone.0231902.ref004","article-title":"An unprecedented coastwide toxic algal bloom linked to anomalous ocean conditions","author":"RM McCabe","year":"2016","journal-title":"Geophysical Research Letters"},{"issue":"DEC","key":"pone.0231902.ref005","doi-asserted-by":"crossref","first-page":"454","DOI":"10.3389\/fmars.2018.00454","article-title":"Mass mortality of cultivated northern bluefin tuna Thunnus thynnus orientalis associated with Chattonella species in Baja California, Mexico","volume":"5","author":"E Garc\u00eda-Mendoza","year":"2018","journal-title":"Frontiers in Marine Science"},{"key":"pone.0231902.ref006","first-page":"26","volume-title":"Florecimientos algales nocivos y su impacto ecol\u00f3gico, econ\u00f3mico y a la salud p\u00fablica en la costa occidental de la pen\u00ednsula de Baja California.","author":"J Medina-Elizalde","year":"2016"},{"issue":"5","key":"pone.0231902.ref007","doi-asserted-by":"crossref","first-page":"902","DOI":"10.1111\/jpy.12101","article-title":"Three novel species in the Pseudo-nitzschia pseudodelicatissima complex: P. batesiana sp. nov., P. lundholmiae sp. nov., and P. fukuyoi sp. nov. (Bacillariophyceae) from the Strait of Malacca, Malaysia.","volume":"49","author":"HC Lim","year":"2013","journal-title":"J Phycol"},{"key":"pone.0231902.ref008","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1111\/jpy.12620","article-title":"Phylogeny and species delineation in the marine diatom Pseudo\u2010nitzchia (Bacillariophyta) using cox1, LSU and ITS2 rRNA genes: a perspective in character evolution.","volume":"54","author":"HC Lim","year":"2018","journal-title":"J Phycol"},{"key":"pone.0231902.ref009","unstructured":"AlgaeBase-World-wide electronic publication, National University of Ireland, Galway [Internet]. 2018 [cited 17 June 2018.]."},{"issue":"4","key":"pone.0231902.ref010","doi-asserted-by":"crossref","first-page":"e0195622","DOI":"10.1371\/journal.pone.0195622","article-title":"A new diatom species P. hallegraeffii sp. nov. belonging to the toxic genus Pseudo-nitzschia (Bacillariophyceae) from the East Australian Current.","volume":"13","author":"PA Ajani","year":"2018","journal-title":"Plos One."},{"key":"pone.0231902.ref011","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.hal.2018.06.001","article-title":"Pseudo-nitzschia, Nitzschia, and domoic acid: new research since 2011","volume":"79","author":"SS Bates","year":"2018","journal-title":"Harmful Algae"},{"key":"pone.0231902.ref012","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.hal.2018.05.003","article-title":"Morphological, molecular and toxigenic characteristics of Namibian Pseudo-nitzschia species\u2013including Pseudo-nitzschia bucculenta sp. nov","volume":"76","author":"FF Gai","year":"2018","journal-title":"Harmful Algae"},{"issue":"6","key":"pone.0231902.ref013","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1111\/jpy.12791","article-title":"Pseudo\u2010nitzschia nanaoensis sp. nov. (Bacillariophyceae) from the Chinese coast of the South China Sea.","volume":"54","author":"Y Li","year":"2018","journal-title":"J Phycol"},{"key":"pone.0231902.ref014","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.hal.2018.06.005","article-title":"Induction of domoic acid production in diatoms\u2014Types of grazers and diatoms are important","volume":"79","author":"N Lundholm","year":"2018","journal-title":"Harmful Algae"},{"issue":"2","key":"pone.0231902.ref015","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1002\/lno.11077","article-title":"Induction of defensive traits in marine plankton\u2014new copepodamide structures","volume":"64","author":"W Grebner","year":"2019","journal-title":"Limnology and Oceanography"},{"issue":"2","key":"pone.0231902.ref016","doi-asserted-by":"crossref","first-page":"168","DOI":"10.2216\/11-37.1","article-title":"Pseudo-nitzschia (Bacillariophyceae) species, domoic acid and amnesic shellfish poisoning: revisiting previous paradigms.","volume":"51","author":"A Lelong","year":"2012","journal-title":"Phycologia"},{"key":"pone.0231902.ref017","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/j.hal.2011.10.025","article-title":"Pseudo-nitzschia physiological ecology, phylogeny, toxicity, monitoring and impacts on ecosystem health.","volume":"14","author":"VL Trainer","year":"2012","journal-title":"Harmful Algae."},{"issue":"2","key":"pone.0231902.ref018","doi-asserted-by":"crossref","first-page":"eaat5096","DOI":"10.1126\/sciadv.aat5096","article-title":"Copepods drive large-scale trait-mediated effects in marine plankton.","volume":"5","author":"E Selander","year":"2019","journal-title":"Sci Adv."},{"issue":"1","key":"pone.0231902.ref019","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1078\/1434-4610-00002","article-title":"The significance of a coincidence between evolutionary landmarks found in mating affinity and a DNA sequence","volume":"151","author":"AW Coleman","year":"2000","journal-title":"Protist"},{"issue":"1","key":"pone.0231902.ref020","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.ympev.2008.10.008","article-title":"Is there a molecular key to the level of \u201cbiological species\u201d in eukaryotes? A DNA guide.","volume":"50","author":"AW Coleman","year":"2009","journal-title":"Mol Phyl Evol"},{"issue":"5","key":"pone.0231902.ref021","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.1111\/j.1529-8817.2012.01213.x","article-title":"Morphology and molecular characterization of Pseudo-nitzschia (Bacillariophyceae) from Malaysian Borneo, including the new species Pseudo-nitzschia circumpora sp. nov","volume":"48","author":"HC Lim","year":"2012","journal-title":"J Phycol"},{"issue":"6","key":"pone.0231902.ref022","doi-asserted-by":"crossref","first-page":"e66726","DOI":"10.1371\/journal.pone.0066726","article-title":"Compensatory base changes in ITS2 secondary structures correlate with the biological species concept despite intragenomic variability in ITS2 sequences\u2013a proof of concept.","volume":"8","author":"M Wolf","year":"2013","journal-title":"PloS One."},{"issue":"4","key":"pone.0231902.ref023","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1111\/jpy.12313","article-title":"A non-toxigenic but morphologically and phylogenetically distinct new species of Pseudo-nitzschia, P. sabit sp. nov. (Bacillariophyceae).","volume":"51","author":"ST Teng","year":"2015","journal-title":"J Phycol"},{"key":"pone.0231902.ref024","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1023\/A:1003471828302","article-title":"Species of the planktonic diatom genus Pseudo-nitzschia of the Pacific coasts of Mexico","volume":"379","author":"DU Hernandez-Becerril","year":"1998","journal-title":"Hydrobiologia"},{"key":"pone.0231902.ref025","unstructured":"Licea S, G\u00f3mez-Aguirre S, Moreno J, editors. The occurrence of the diatom genus Pseudo-nitzschia Peragallo in Mexican coastal waters, 1979\u201320002000; Proc. 16th Int. Diatom Symposium: Athens & Aegean Islands"},{"key":"pone.0231902.ref026","unstructured":"Cabrales-Talavera G. Distribuci\u00f3n espacial y temporal de diatomeas del g\u00e9nero Pseudo-nitzschia spp y concentraci\u00f3n de \u00e1cido domoico en la regi\u00f3n de la Bah\u00eda de Todos Santos, BC, M\u00e9xico: MS thesis. Centro de Investigaci\u00f3n Cient\u00edfica y de Educaci\u00f3n Superior de Ensenada (CICESE), Ensenada; 2010."},{"key":"pone.0231902.ref027","article-title":"Seasonal diversity of Pseudo-nitzschia spp in the central zone of the Mexican Tropical Pacific, Manzanillo, Colima","author":"S Quijano-Scheggia","year":"2010","journal-title":"14th Int Conf Harmful Algae"},{"issue":"2","key":"pone.0231902.ref028","doi-asserted-by":"crossref","first-page":"189","DOI":"10.4067\/S0718-19572011000200008","article-title":"First report of Pseudo-nitzschia brasiliana and P. micropora (Bacillariophycea) found in Cuyutlan Lagoon, Mexico","volume":"46","author":"S Quijano-Scheggia","year":"2011","journal-title":"Rev Biol Mar Oceanog"},{"key":"pone.0231902.ref029","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.hal.2011.09.004","article-title":"Growth and domoic acid content of Pseudo-nitzschia australis isolated from northwestern Baja California, Mexico, cultured under batch conditions at different temperatures and two Si:NO3 ratios","volume":"12","author":"IS Santiago-Morales","year":"2011","journal-title":"Harmful Algae"},{"issue":"4","key":"pone.0231902.ref030","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1515\/bot-2012-0176","article-title":"The genus Pseudo-nitzschia (Bacillariophyceae) in Manzanillo and Santiago Bays, Colima, Mexico.","volume":"56","author":"M Rivera-Vilarelle","year":"2013","journal-title":"Bot Mar"},{"issue":"1","key":"pone.0231902.ref031","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1080\/0269249X.2018.1426633","article-title":"Description of Pseudo-nitzschia cuspidata var. manzanillensis var. nov. (Bacillariophyceae): morphology and molecular characterization of a variety from the central Mexican pacific.","volume":"33","author":"M Rivera-Vilarelle","year":"2018","journal-title":"Diatom Res"},{"key":"pone.0231902.ref032","first-page":"1","article-title":"Nitschia and Fragilariopsis species studied in the light and electron microscopes. II The group Pseudonitzschia.","volume":"18","author":"GR Hasle","year":"1965","journal-title":"Skrifter utgitt av Det Norske Videnskaps-Akademi i Oslo I Matematisk-Naturvidenskapelige Klasse Ny Serie."},{"key":"pone.0231902.ref033","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1515\/botm.1994.37.5.397","article-title":"Species of the diatom genus Pseudo-nitzschia in Australian waters.","volume":"37","author":"GM Hallegraeff","year":"1994","journal-title":"Bot Mar"},{"key":"pone.0231902.ref034","first-page":"364","article-title":"Monthly monitoring of domoic acid producer Pseudo-nitzschia multiseries (Hasle) Hasle using species-specific DNA probes and WGA lectins and abundance of Pseudo-nitzschia species (Bacillariophyceae) from Chinhae Bay, Korea.","volume":"45","author":"ES Cho","year":"2002","journal-title":"Bot Mar"},{"issue":"1\u20132","key":"pone.0231902.ref035","first-page":"123","article-title":"Diversity, taxonomy and biogeographical distribution of the genus Pseudo-nitzschia (Bacillariophyceae) in Guangdong coastal waters, South China Sea","volume":"95","author":"SH L\u00fc","year":"2012","journal-title":"Nova Hedwigia"},{"key":"pone.0231902.ref036","unstructured":"Moschandreou K, Nikolaidis G, editors. Pseudo-nitzschia Peragallo (Bacillariophyceae) in Greek coastal waters. 12th International Conference on Harmful Algae 2006; Copenhagen, Denmark."},{"key":"pone.0231902.ref037","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/0269249X.2016.1247018","article-title":"Morphological and toxicological studies of Pseudo-nitzschia species from the central coast of Peru.","volume":"31","author":"C Tenorio","year":"2016","journal-title":"Diatom Res."},{"issue":"2","key":"pone.0231902.ref038","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1007\/s10152-015-0428-5","article-title":"Diversity and seasonality of Pseudo-nitzschia (Peragallo) at two North Sea time-series monitoring sites.","volume":"69","author":"E Bresnan","year":"2015","journal-title":"Helgoland Mar Res"},{"issue":"103","key":"pone.0231902.ref039","first-page":"103:39","article-title":"Diversity and toxicity of the diatom Pseudo-nitzschia Peragallo in the Gulf of Maine, Northwestern Atlantic Ocean","volume":"II","author":"LF Fernandes","year":"2014","journal-title":"Deep-Sea Res"},{"issue":"2","key":"pone.0231902.ref040","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1007\/BF02367149","article-title":"A review of Pseudo-nitzschia, with special reference to the Skagerrak, North Atlantic, and adjacent waters.","volume":"50","author":"G Hasle","year":"1996","journal-title":"Helgol\u00e4nder Meeresuntersuchungen."},{"issue":"1","key":"pone.0231902.ref041","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1080\/0269249X.2009.9705782","article-title":"Diversity and abundance of potentially toxic Pseudo-nitzschia Peragallo in Aveiro coastal lagoon, Portugal and description of a new variety, P. pungens var. aveirensis var. nov","volume":"24","author":"CI Churro","year":"2009","journal-title":"Diatom Res"},{"key":"pone.0231902.ref042","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.hal.2003.07.001","article-title":"Diversity of the diatom genus Pseudo-nitzschia Peragallo in the Quoddy Region of the Bay of Fundy, Canada","volume":"4","author":"I Kaczmarska","year":"2005","journal-title":"Harmful Algae"},{"issue":"3","key":"pone.0231902.ref043","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1080\/09670262.2012.713998","article-title":"Inter- and intra-specific diversity of Pseudo-nitzschia (Bacillariophyceae) in the northeastern Mediterranean.","volume":"47","author":"KK Moschandreou","year":"2012","journal-title":"European Journal of Phycology"},{"key":"pone.0231902.ref044","first-page":"5","article-title":"Identification of Pseudo-nitzschia multistriata and P. subpacifica from French waters. Were they part of the cryptic flora?","volume":"35","author":"E Nezan","year":"2007","journal-title":"Harmful Algae News"},{"key":"pone.0231902.ref045","doi-asserted-by":"crossref","first-page":"1192","DOI":"10.1111\/jpy.12130","article-title":"The genus Pseudo-nitzschia (Bacillariophyceae) in a temperate estuary with description of two new species: Pseudo-nitzschia plurisecta sp. nov. and Pseudo-nitzschia abrensis sp. nov","volume":"49","author":"E Orive","year":"2013","journal-title":"J Phycol"},{"issue":"52","key":"pone.0231902.ref046","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1111\/jpy.12448","article-title":"High diversity of Pseudo-nitzschia along the northern coast of Sarawak (Malaysian Borneo), with descriptions of P. bipertita sp. nov. and P. limii sp. nov. (Bacillariophyceae).","author":"ST Teng","year":"2016","journal-title":"J Phycol"},{"issue":"1","key":"pone.0231902.ref047","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1046\/j.1529-8817.2002.01106.x","article-title":"Morphological, toxicological, and genetic differences among Pseudo-nitzschia (Bacillariophyceae) species in inland embayments and outer coastal waters of Washington State, USA","volume":"38","author":"CM Stehr","year":"2002","journal-title":"J Phycol"},{"key":"pone.0231902.ref048","doi-asserted-by":"crossref","first-page":"55","DOI":"10.3318\/BIOE.2004.104.1.55","article-title":"Occurrence of species from the genus Pseudo-nitzschia Peragallo in Irish waters","volume":"104B","author":"C Cusack","year":"2004","journal-title":"Proc Royal Irish Acad"},{"issue":"3","key":"pone.0231902.ref049","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1111\/j.1529-8817.2008.00526.x","article-title":"Identification and assessment of domoic acid production in oceanic Pseudo-nitzschia (Bacillariophyceae) from iron-limited waters in the Northeast Subarctic Pacific.","volume":"44","author":"A Marchetti","year":"2008","journal-title":"Journal of Phycology"},{"issue":"6","key":"pone.0231902.ref050","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1007\/s10811-012-9803-7","article-title":"Genetic diversity of Pseudo-nitzschia brasiliana (Bacillariophyceae) from Malaysia.","volume":"24","author":"H-C Lim","year":"2012","journal-title":"J Appl Phycol"},{"key":"pone.0231902.ref051","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/S1568-9883(02)00014-8","article-title":"Are most of the domoic acid-producing Pseudo-nitzschia, bloom dynamics, phytoplankton communityspecies of the diatom genus Pseudo-nitzschia cosmopolites?","volume":"1","author":"GR Hasle","year":"2002","journal-title":"Harmful Algae"},{"key":"pone.0231902.ref052","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1046\/j.1529-8817.2003.02031.x","article-title":"A study of the Pseudo-nitzschia pseudodelicatissima\/cuspidata complex (Bacillariophyceae): What is P. pseudodelicatissima?","volume":"39","author":"N Lundholm","year":"2003","journal-title":"J Phycol"},{"key":"pone.0231902.ref053","first-page":"1","author":"A Amato","year":"2019","journal-title":"Cryptic Diversity: a Long-lasting Issue for Diatomologists"},{"issue":"10","key":"pone.0231902.ref054","doi-asserted-by":"crossref","first-page":"3322","DOI":"10.1093\/nar\/gkm233","article-title":"Pan-eukaryote ITS2 homologies revealed by RNA secondary structure","volume":"35","author":"AW Coleman","year":"2007","journal-title":"Nucleic Acids Research"},{"issue":"5","key":"pone.0231902.ref055","doi-asserted-by":"crossref","first-page":"487","DOI":"10.2216\/07-92.1","article-title":"Morphology, phylogeny, and sexual cycle of Pseudo-nitzschia mannii sp. nov. (Bacillariophyceae): a pseudo-cryptic species within the P. pseudodelicatissima complex.","volume":"47","author":"A Amato","year":"2008","journal-title":"Phycologia"},{"key":"pone.0231902.ref056","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.toxicon.2009.07.005","article-title":"Intracellular domoic acid production in Pseudo-nitzschia multistriata isolated from the Gulf of Naples (Tyrrhenian Sea, Italy).","volume":"55","author":"A Amato","year":"2010","journal-title":"Toxicon"},{"key":"pone.0231902.ref057","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.ecss.2018.05.013","article-title":"Coastal eutrophication and freshening: Impacts on Pseudo-nitzschia abundance and domoic acid allelopathy.","volume":"209","author":"E Van Meerssche","year":"2018","journal-title":"Estuar Coast Shelf Sci"},{"key":"pone.0231902.ref058","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.hal.2017.03.001","article-title":"Inter and intra-specific growth and domoic acid production in relation to nutrient ratios and concentrations in Pseudo-nitzschia: phosphate an important factor","volume":"64","author":"KA Lema","year":"2017","journal-title":"Harmful algae"},{"key":"pone.0231902.ref059","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.hal.2017.06.004","article-title":"Understanding the blob bloom: Warming increases toxicity and abundance of the harmful bloom diatom Pseudo-nitzschia in California coastal waters","volume":"67","author":"Z Zhu","year":"2017","journal-title":"Harmful Algae"},{"issue":"6409","key":"pone.0231902.ref060","doi-asserted-by":"crossref","first-page":"1356","DOI":"10.1126\/science.aau0382","article-title":"Biosynthesis of the neurotoxin domoic acid in a bloom-forming diatom","volume":"361","author":"JK Brunson","year":"2018","journal-title":"Science"},{"key":"pone.0231902.ref061","doi-asserted-by":"crossref","first-page":"83","DOI":"10.3354\/meps220083","article-title":"Pseudo-nitzschia sp cf. pseudodelicatissima\u2014a confirmed producer of domoic acid from the northern Gulf of Mexico","volume":"220","author":"YL Pan","year":"2001","journal-title":"Mar Ecol Prog Ser"},{"key":"pone.0231902.ref062","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.toxicon.2018.10.003","article-title":"Evaluation of intracellular and extracellular domoic acid content in Pseudo-nitzchia multiseries cell cultures under different light regimes","volume":"155","author":"L Godinho","year":"2018","journal-title":"Toxicon"},{"key":"pone.0231902.ref063","doi-asserted-by":"crossref","first-page":"234","DOI":"10.2216\/i0031-8884-32-3-234.1","article-title":"Stichochrysis immobilis is a diatom, not a chrysophyte.","volume":"32","author":"RRL Guillard","year":"1993","journal-title":"Phycologia"},{"key":"pone.0231902.ref064","author":"B Karlson","year":"2010","journal-title":"Microscopic and molecular methods for quantitative phytoplankton analysis"},{"issue":"5","key":"pone.0231902.ref065","doi-asserted-by":"crossref","first-page":"480","DOI":"10.2216\/i0031-8884-41-5-480.1","article-title":"Morphology, phylogeny and taxonomy of species within the Pseudo-nitzschia americana complex (Bacillariophyceae) with descriptions of two new species, Pseudo-nitzschia brasiliana and Pseudo-nitzschia linea.","volume":"41","author":"N Lundholm","year":"2002","journal-title":"Phycologia"},{"key":"pone.0231902.ref066","author":"JJ Doyle","year":"1990","journal-title":"Isolation of plant DNA from fresh tissue"},{"key":"pone.0231902.ref067","first-page":"315","volume-title":"Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics.","author":"TJ White","year":"1990"},{"issue":"2","key":"pone.0231902.ref068","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.gene.2014.05.065","article-title":"ITS2, 18S, 16S or any other RNA\u2014simply aligning sequences and their individual secondary structures simultaneously by an automatic approach","volume":"546","author":"M Wolf","year":"2014","journal-title":"Gene"},{"issue":"4","key":"pone.0231902.ref069","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1093\/bioinformatics\/btq706","article-title":"Phangorn: phylogenetic analysis in R","volume":"27","author":"KP Schliep","year":"2011","journal-title":"Bioinformatics"},{"key":"pone.0231902.ref070","doi-asserted-by":"crossref","first-page":"1572","DOI":"10.1093\/bioinformatics\/btg180","article-title":"MrBayes 3: Bayesian phylogenetic inference under mixed models","volume":"19","author":"F Ronquist","year":"2003","journal-title":"Bioinformatics"},{"issue":"6","key":"pone.0231902.ref071","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms","volume":"35","author":"S Kumar","year":"2018","journal-title":"Mol Biol Evol"},{"issue":"11","key":"pone.0231902.ref072","doi-asserted-by":"crossref","first-page":"1616","DOI":"10.1261\/rna.2144205","article-title":"Homology modeling revealed more than 20,000 rRNA internal transcribed spacer 2 (ITS2) secondary structures.","volume":"11","author":"M Wolf","year":"2005","journal-title":"RNA"},{"issue":"suppl 2","key":"pone.0231902.ref073","doi-asserted-by":"crossref","first-page":"W704","DOI":"10.1093\/nar\/gkl129","article-title":"The internal transcribed spacer 2 database\u2014a web server for (not only) low level phylogenetic analyses.","volume":"34","author":"J Schultz","year":"2006","journal-title":"Nucleic Acids Res"},{"issue":"suppl 1","key":"pone.0231902.ref074","first-page":"D377","article-title":"The ITS2 Database II: homology modelling RNA structure for molecular systematics","volume":"36","author":"C Selig","year":"2008","journal-title":"Nucleic Acids Res"},{"issue":"suppl 1","key":"pone.0231902.ref075","doi-asserted-by":"crossref","first-page":"D275","DOI":"10.1093\/nar\/gkp966","article-title":"The ITS2 Database III\u2014sequences and structures for phylogeny","volume":"38","author":"C Koetschan","year":"2010","journal-title":"Nucleic Acids Res"},{"issue":"11","key":"pone.0231902.ref076","doi-asserted-by":"crossref","first-page":"3030","DOI":"10.1093\/molbev\/msv174","article-title":"ITS2 database V: Twice as much","volume":"32","author":"MJ Ankenbrand","year":"2015","journal-title":"Mol Biol Evol"},{"issue":"15","key":"pone.0231902.ref077","doi-asserted-by":"crossref","first-page":"1974","DOI":"10.1093\/bioinformatics\/btp250","article-title":"VARNA: Interactive drawing and editing of the RNA secondary structure","volume":"25","author":"K Darty","year":"2009","journal-title":"Bioinformatics"},{"key":"pone.0231902.ref078","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1186\/1471-2105-7-498","article-title":"4SALE\u2013A tool for synchronous RNA sequence and secondary structure alignment and editing","volume":"7","author":"PN Seibel","year":"2006","journal-title":"BMC Bioinformatics"},{"key":"pone.0231902.ref079","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1007\/PL00006143","article-title":"The internal transcriber spacer 2 exhibits a common secondary structure in green algae and flowering plants","volume":"44","author":"JC Mai","year":"1997","journal-title":"J Mol Evol"},{"issue":"2","key":"pone.0231902.ref080","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.protis.2006.10.001","article-title":"Reproductive isolation among sympatric cryptic species in marine diatoms","volume":"158","author":"A Amato","year":"2007","journal-title":"Protist"},{"key":"pone.0231902.ref081","volume-title":"A practical handbook of sea water analysis.","author":"JD Strickland","year":"1972"},{"key":"pone.0231902.ref082","first-page":"345","volume-title":"Methods of sea water analysis","author":"K Grasshoff","year":"1983"},{"issue":"3","key":"pone.0231902.ref083","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/S0272-7714(05)80014-9","article-title":"Changes in nutrient structure of river-dominated coastal waters: stoichiometric nutrient balance and its consequences","volume":"40","author":"D Justic","year":"1995","journal-title":"Estuar Coast Shelf Sci"},{"issue":"32","key":"pone.0231902.ref084","doi-asserted-by":"crossref","first-page":"6003","DOI":"10.1016\/j.chroma.2009.06.050","article-title":"Analysis of trace levels of domoic acid in seawater and plankton by liquid chromatography without derivatization, using UV or mass spectrometry detection","volume":"1216","author":"LLJ Mafra","year":"2009","journal-title":"J Chromatogr A"},{"key":"pone.0231902.ref085","unstructured":"StatSoft I. STATISTICA (data analysis software system), version 6. StatSoft, Inc 2003 www.statsoft.com.; 2003."}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0231902","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,3,10]],"date-time":"2021-03-10T22:57:36Z","timestamp":1615417056000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0231902"}},"subtitle":[],"editor":[{"given":"Alberto","family":"Amato","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,4,24]]},"references-count":85,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2020,4,24]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0231902","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,24]]}}}