{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T03:31:26Z","timestamp":1782963086456,"version":"3.54.5"},"reference-count":32,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2020,5,1]],"date-time":"2020-05-01T00:00:00Z","timestamp":1588291200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["OPP1154992"],"award-info":[{"award-number":["OPP1154992"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["OPP1053230"],"award-info":[{"award-number":["OPP1053230"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0232325","type":"journal-article","created":{"date-parts":[[2020,5,1]],"date-time":"2020-05-01T17:29:45Z","timestamp":1588354185000},"page":"e0232325","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":15,"title":["Selection and exploitation of prevalent, tandemly repeated\u00a0genomic targets for improved real-time PCR-based detection of Wuchereria bancrofti and Plasmodium falciparum\u00a0in mosquitoes"],"prefix":"10.1371","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5703-5675","authenticated-orcid":true,"given":"Michael F.","family":"Zulch","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8447-7425","authenticated-orcid":true,"given":"Nils","family":"Pilotte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica R.","family":"Grant","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7862-4874","authenticated-orcid":true,"given":"Corrado","family":"Minetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa J.","family":"Reimer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven A.","family":"Williams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2020,5,1]]},"reference":[{"issue":"1","key":"pone.0232325.ref001","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1186\/s40249-016-0151-8","article-title":"Malaria: Global progress 2000\u20132015 and future challenges","volume":"5","author":"RE Cibulskis","year":"2016","journal-title":"Infect Dis Poverty"},{"issue":"11","key":"pone.0232325.ref002","doi-asserted-by":"crossref","first-page":"e1002450","DOI":"10.1371\/journal.pmed.1002456","article-title":"malERA: An updated research agenda for malaria elimination and eradication","volume":"14","author":"RN Rabinovich","year":"2017","journal-title":"PLoS Med"},{"issue":"40","key":"pone.0232325.ref003","first-page":"594","article-title":"Global programme to eliminate lymphatic filariasis: progress report, 2017","volume":"92","author":"WHO\/Department of Control of Neglected Tropical Diseases","year":"2018","journal-title":"Wkly Epidemiol Rec"},{"key":"pone.0232325.ref004","volume-title":"Lymphatic Filariasis: Monitoring and Epidemiological Assessment of Mass Drug Administration\u2013A Manual for National Elimination Programmes","author":"World Health Organization","year":"2011"},{"key":"pone.0232325.ref005","volume-title":"Universal Access to Malaria Diagnostic Testing: An Operational Manual","author":"World Health Organization","year":"2013"},{"issue":"7","key":"pone.0232325.ref006","doi-asserted-by":"crossref","first-page":"2009","DOI":"10.1128\/JCM.02562-16","article-title":"Update on Malaria Diagnostics and Test Utilization","volume":"55","author":"BA Mathison","year":"2017","journal-title":"J Clin Microbiol"},{"issue":"12","key":"pone.0232325.ref007","doi-asserted-by":"crossref","first-page":"e2584","DOI":"10.1371\/journal.pntd.0002584","article-title":"Transmission assessment surveys (TAS) to define endpoints for lymphatic filariasis mass drug administration: a multicenter evaluation","volume":"7","author":"BK Chu","year":"2013","journal-title":"PLoS Negl Trop Dis"},{"issue":"7580","key":"pone.0232325.ref008","doi-asserted-by":"crossref","first-page":"S86","DOI":"10.1038\/nature16039","article-title":"Comparison of diagnostics for the detection of asymptomatic Plasmodium falciparum infections to inform control and elimination strategies","volume":"528","author":"L Wu","year":"2015","journal-title":"Nature"},{"issue":"2","key":"pone.0232325.ref009","doi-asserted-by":"crossref","first-page":"e0005097","DOI":"10.1371\/journal.pntd.0005097","article-title":"Costs of Transmission Assessment Surveys to Provide Evidence for the Elimination of Lymphatic Filariasis","volume":"11","author":"MA Brady","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"issue":"6","key":"pone.0232325.ref010","doi-asserted-by":"crossref","first-page":"64","DOI":"10.4103\/nmj.NMJ_165_18","article-title":"Evaluation of Diagnostic Accuracy of Rapid Diagnostic Test for Malaria Diagnosis among Febrile Children in Calabar, Nigeria","volume":"59","author":"AA Iwuafor","year":"2018","journal-title":"Niger Med J"},{"issue":"4","key":"pone.0232325.ref011","doi-asserted-by":"crossref","first-page":"809","DOI":"10.4269\/ajtmh.19-0394","article-title":"Diagnostic Performance of Malaria Rapid Diagnostic Test and Microscopy Compared with PCR for Detection of Plasmodium falciparum Infections among Primary Schoolchildren in Kibiti District, Eastern Tanzania: An Area with Moderate Malaria Transmission","volume":"101","author":"B Ngasala","year":"2019","journal-title":"Am J Trop Med Hyg"},{"key":"pone.0232325.ref012","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1186\/s13071-016-1556-8","article-title":"Further evidence of the cross-reactivity of the Binax NOW Filariasis ICT cards to non-Wuchereria bancrofti filariae: experimental studies with Loa loa and Onchocerca ochengi","volume":"9","author":"S Wanji","year":"2016","journal-title":"Parasit Vectors"},{"key":"pone.0232325.ref013","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1186\/1756-3305-7-146","article-title":"Real-time PCR detection of the HhaI tandem DNA repeat in pre- and post-patent Brugia malayi infections: a study in Indonesian transmigrants","volume":"7","author":"A Albers","year":"2014","journal-title":"Parasit Vectors"},{"issue":"8","key":"pone.0232325.ref014","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/j.pt.2015.04.006","article-title":"Expanding the MDx toolbox for filarial diagnosis and surveillance","volume":"31","author":"A Alhassan","year":"2015","journal-title":"Trends Parasitol"},{"issue":"4","key":"pone.0232325.ref015","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/j.trstmh.2008.07.012","article-title":"A qPCR-based Multiplex Assay for Detection of Wuchereria bancrofti, Plasmodium falciparum, and Plasmodium vivax DNA","volume":"103","author":"RU Rao","year":"2009","journal-title":"Trans R Soc Trop Med Hyg"},{"issue":"5","key":"pone.0232325.ref016","doi-asserted-by":"crossref","first-page":"826","DOI":"10.4269\/ajtmh.2006.74.826","article-title":"A Real-time PCR-based Assay for Detection of Wuchereria bancrofti DNA in Blood and Mosquitoes","volume":"74","author":"RU Rao","year":"2006","journal-title":"Am J Trop Med Hyg"},{"key":"pone.0232325.ref017","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1186\/1475-2875-7-177","article-title":"PCR-based detection of Plasmodium in Anopheles mosquitoes: a comparison of a new high-throughput assay with existing methods","volume":"7","author":"C Bass","year":"2008","journal-title":"Malar J"},{"issue":"4","key":"pone.0232325.ref018","doi-asserted-by":"crossref","first-page":"e0004641","DOI":"10.1371\/journal.pntd.0004641","article-title":"A Novel Xenomonitoring Technique Using Mosquito Excreta\/Feces for the Detection of Filarial Parasites and Malaria","volume":"10","author":"N Pilotte","year":"2016","journal-title":"PLoS Negl Trop Dis"},{"issue":"4","key":"pone.0232325.ref019","doi-asserted-by":"crossref","first-page":"357","DOI":"10.4269\/ajtmh.1996.54.357","article-title":"A polymerase chain reaction assay for detection of the parasite W. bancrofti in human blood samples","volume":"54","author":"M Zhong","year":"1996","journal-title":"Am J Trop Med Hyg"},{"issue":"5","key":"pone.0232325.ref020","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1016\/0890-8508(90)90031-T","article-title":"PCR detection of Plasmodium falciparum by oligonucleotide probes","volume":"4","author":"G Jaureguiberry","year":"1990","journal-title":"Mol Cell Probes"},{"key":"pone.0232325.ref021","doi-asserted-by":"crossref","first-page":"883","DOI":"10.3389\/fgene.2019.00883","article-title":"A Case for Using Genomics and a Bioinformatics Pipeline to Develop Sensitive and Species-Specific PCR-Based Diagnostics for Soil-Transmitted Helminths","volume":"10","author":"JR Grant","year":"2019","journal-title":"Front Genet"},{"issue":"6","key":"pone.0232325.ref022","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1093\/bioinformatics\/btt054","article-title":"RepeatExplorer: a Galaxy-based web server for genome-wide characterization of eukaryotic repetitive elements from next-generation sequence reads","volume":"29","author":"P Nov\u00e1k","year":"2013","journal-title":"Bioinformatics"},{"issue":"12","key":"pone.0232325.ref023","doi-asserted-by":"crossref","first-page":"e111","DOI":"10.1093\/nar\/gkx257","article-title":"TAREAN: a computational tool for identification and characterization of satellite DNA from unassembled short reads","volume":"45","author":"P Nov\u00e1k","year":"2017","journal-title":"Nucleic Acids Res"},{"issue":"3","key":"pone.0232325.ref024","doi-asserted-by":"crossref","first-page":"e0004578","DOI":"10.1371\/journal.pntd.0004578","article-title":"Improved PCR-Based Detection of Soil Transmitted Helminth Infections Using a Next-Generation Sequencing Approach to Assay Design","volume":"10","author":"N Pilotte","year":"2016","journal-title":"PLoS Negl Trop Dis"},{"issue":"7","key":"pone.0232325.ref025","doi-asserted-by":"crossref","first-page":"e0005734","DOI":"10.1371\/journal.pntd.0005734","article-title":"A novel, species-specific, real-time PCR assay for the detection of the emerging zoonotic parasite Ancylostoma ceylanicum in human stool","volume":"11","author":"M Papaiakovou","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"issue":"7","key":"pone.0232325.ref026","doi-asserted-by":"crossref","first-page":"e0007593","DOI":"10.1371\/journal.pntd.0007593","article-title":"Targeting a highly repeated germline DNA sequence for improved real-time PCR-based detection of Ascaris infection in human stool","volume":"13","author":"N Pilotte","year":"2019","journal-title":"PLoS Negl Trop Dis"},{"issue":"4","key":"pone.0232325.ref027","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1016\/S0035-9203(96)90515-5","article-title":"A polymerase chain reaction assay for the detection of Wuchereria bancrofti in blood samples from French Polynesia","volume":"90","author":"L Nicolas","year":"1996","journal-title":"Trans R Soc Trop Med Hyg"},{"issue":"8","key":"pone.0232325.ref028","doi-asserted-by":"crossref","first-page":"e71539","DOI":"10.1371\/journal.pone.0071539","article-title":"Multiplex qPCR for Detection and Absolute Quantification of Malaria","volume":"8","author":"E Kamau","year":"2013","journal-title":"PLoS One"},{"issue":"1\u20132","key":"pone.0232325.ref029","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.molbiopara.2013.05.001","article-title":"A TaqMan-based multiplex real-time PCR assay for the simultaneous detection of Wuchereria bancrofti and Brugia malayi","volume":"189","author":"N Pilotte","year":"2013","journal-title":"Mol Biochem Parasitol"},{"issue":"3","key":"pone.0232325.ref030","doi-asserted-by":"crossref","first-page":"314","DOI":"10.4269\/ajtmh.1994.51.314","article-title":"A polymerase chain reaction assay for the detection of Brugia malayi in blood","volume":"51","author":"MR Lizotte","year":"1994","journal-title":"Am J Trop Med Hyg"},{"issue":"2","key":"pone.0232325.ref031","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.ijppaw.2018.04.001","article-title":"A novel quantitative real-time PCR diagnostic assay for seal heartworm (Acanthocheilonema spirocauda) provides evidence for possible infection in the grey seal (Halichoerus grypus)","volume":"7","author":"CD Keroack","year":"2018","journal-title":"Int J Parasitol Parasites Wildl"},{"key":"pone.0232325.ref032","doi-asserted-by":"crossref","DOI":"10.30875\/f309483f-en","volume-title":"World Malaria Report: 2018","author":"World Health Organization","year":"2018"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0232325","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,1]],"date-time":"2020-05-01T17:30:08Z","timestamp":1588354208000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0232325"}},"subtitle":[],"editor":[{"given":"Luzia Helena","family":"Carvalho","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,5,1]]},"references-count":32,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2020,5,1]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0232325","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,1]]}}}