{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T07:03:50Z","timestamp":1777100630255,"version":"3.51.4"},"reference-count":40,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2021,2,19]],"date-time":"2021-02-19T00:00:00Z","timestamp":1613692800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000094","name":"Ontario Ministry of Agriculture, Food and Rural Affairs","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000094","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Boehringer-Ingelheim Vetmedica"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    This study evaluated the effects of treatment with meloxicam (a non-steroidal anti-inflammatory drug), parity, and blood progesterone concentration on the dynamics of the uterine microbiota of 16 clinically healthy postpartum dairy cows. Seven primiparous and 9 multiparous postpartum Holstein cows either received meloxicam (0.5 mg\/kg SC, n = 7 cows) once daily for 4 days (10 to 13 days in milk (DIM)) or were untreated (n = 9 cows). Endometrial cytology samples were collected by cytobrush at 10, 21, and 35 DIM, from which the microbiota analysis was conducted using 16S rRNA gene sequence analysis. A radioimmunoassay was used to measure progesterone concentration in blood serum samples at 35 DIM and cows were classified as \u02c3 1 ng\/mL (n = 10) or \u2264 1 ng\/mL (n = 6). Alpha diversity for bacterial genera (Chao1, Shannon-Weiner, and Camargo\u2019s evenness indices) were not affected by DIM, meloxicam treatment, parity, or progesterone category. For beta diversity (genera level), principal coordinate analysis (Bray-Curtis) showed differences in microbiota between parity groups. At the phylum level, the relative abundance of Actinobacteria was greater in primiparous than multiparous cows. At the genus level, there was lesser relative abundance of\n                    <jats:italic>Bifidobacterium<\/jats:italic>\n                    ,\n                    <jats:italic>Lactobacillus<\/jats:italic>\n                    ,\n                    <jats:italic>Neisseriaceae<\/jats:italic>\n                    ,\n                    <jats:italic>Paracoccus<\/jats:italic>\n                    ,\n                    <jats:italic>Staphylococcus<\/jats:italic>\n                    , and\n                    <jats:italic>Streptococcus<\/jats:italic>\n                    and greater relative abundance of\n                    <jats:italic>Bacillus<\/jats:italic>\n                    and\n                    <jats:italic>Fusobacterium<\/jats:italic>\n                    in primiparous than multiparous cows. Bray-Curtis dissimilarity did not differ by DIM at sampling, meloxicam treatment, or progesterone category at 35 DIM. In conclusion, uterine bacterial composition was not different at 10, 21, or 35 DIM, and meloxicam treatment or progesterone category did not affect the uterine microbiota in clinically healthy postpartum dairy cows. Primiparous cows presented a different composition of uterine bacteria than multiparous cows. The differences in microbiota associated with parity might be attributable to changes that occur consequent to the first calving, but this hypothesis should be investigated further.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0233943","type":"journal-article","created":{"date-parts":[[2021,2,19]],"date-time":"2021-02-19T22:37:42Z","timestamp":1613774262000},"page":"e0233943","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":26,"title":["Effects of parity, blood progesterone, and non-steroidal anti-inflammatory treatment on the dynamics of the uterine microbiota of healthy postpartum dairy cows"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5305-2133","authenticated-orcid":true,"given":"O.","family":"Bogado Pascottini","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. F. 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