{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T16:50:16Z","timestamp":1791910216607,"version":"4.3.4"},"reference-count":37,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2020,6,5]],"date-time":"2020-06-05T00:00:00Z","timestamp":1591315200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010665","name":"H2020 Marie Sk\u0142odowska-Curie Actions","doi-asserted-by":"publisher","award":["658126"],"award-info":[{"award-number":["658126"]}],"id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010666","name":"H2020 Research Infrastructures","doi-asserted-by":"publisher","award":["730924"],"award-info":[{"award-number":["730924"]}],"id":[{"id":"10.13039\/100010666","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0234344","type":"journal-article","created":{"date-parts":[[2020,6,5]],"date-time":"2020-06-05T17:39:28Z","timestamp":1591378768000},"page":"e0234344","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":11,"title":["The origin of N isotopic discrimination and its relationship with feed efficiency in fattening yearling bulls is diet-dependent"],"prefix":"10.1371","volume":"15","author":[{"given":"Sayyed Mahmoud","family":"Nasrollahi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah Jade","family":"Meale","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3883-0937","authenticated-orcid":true,"given":"Diego P.","family":"Morgavi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne Marie","family":"Schiphorst","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard J.","family":"Robins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Ortigues-Marty","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9486-8238","authenticated-orcid":true,"given":"Gonzalo","family":"Cantalapiedra-Hijar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2020,6,5]]},"reference":[{"key":"pone.0234344.ref001","doi-asserted-by":"crossref","first-page":"1705","DOI":"10.1017\/S0007114513004078","article-title":"Plasma nitrogen isotopic fractionation and feed efficiency in growing beef heifers","volume":"111","author":"N Wheadon","year":"2014","journal-title":"Br J Nutr"},{"key":"pone.0234344.ref002","doi-asserted-by":"crossref","first-page":"1158","DOI":"10.1017\/S0007114514004449","article-title":"Diet\u2013animal fractionation of nitrogen stable isotopes reflects the efficiency of nitrogen assimilation in ruminants","volume":"113","author":"G Cantalapiedra-Hijar","year":"2015","journal-title":"Br J Nutr"},{"key":"pone.0234344.ref003","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/0016-7037(81)90244-1","article-title":"Influence of diet on the distribution of nitrogen isotopes in animals","volume":"45","author":"MJ DeNiro","year":"1981","journal-title":"Geochim Cosmochim Acta"},{"key":"pone.0234344.ref004","doi-asserted-by":"crossref","first-page":"1827","DOI":"10.1017\/S1751731117003391","article-title":"Nitrogen isotopic fractionation as a biomarker for nitrogen use efficiency in ruminants: a meta-analysis","volume":"12","author":"G Cantalapiedra-Hijar","year":"2018","journal-title":"Animal"},{"key":"pone.0234344.ref005","doi-asserted-by":"crossref","first-page":"2143","DOI":"10.1016\/0016-7037(86)90068-2","article-title":"Kinetic fractionation of stable nitrogen isotopes during amino acid transamination","volume":"50","author":"SA Macko","year":"1986","journal-title":"Geochim Cosmochim Acta"},{"key":"pone.0234344.ref006","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1016\/S1095-6433(98)01016-2","article-title":"Natural abundance variations in stable isotopes and their potential uses in animal physiological ecology","volume":"119","author":"LZ Gannes","year":"1998","journal-title":"Comp Biochem Physiol A Mol Integr Physiol"},{"key":"pone.0234344.ref007","doi-asserted-by":"crossref","first-page":"4814","DOI":"10.1021\/bi00413a035","article-title":"Use of primary deuterium and nitrogen-15 isotope effects to deduce the relative rates of steps in the mechanisms of alanine and glutamate dehydrogenases","volume":"27","author":"PM Weiss","year":"1988","journal-title":"Biochemistry"},{"key":"pone.0234344.ref008","volume-title":"Harper's Illustrated Biochemistry","author":"RK Murray","year":"2009","edition":"28"},{"key":"pone.0234344.ref009","doi-asserted-by":"crossref","first-page":"8696","DOI":"10.1073\/pnas.1216683110","article-title":"Mechanism for nitrogen isotope fractionation during ammonium assimilation by Escherichia coli K12","volume":"110","author":"J Vo","year":"2013","journal-title":"Proc Natl Acad Sci USA"},{"key":"pone.0234344.ref010","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1016\/0146-6380(84)90100-1","article-title":"Microbial alteration of stable nitrogen and carbon isotopic compositions of organic matter","volume":"6","author":"SA Macko","year":"1984","journal-title":"Organic Geochemistry"},{"key":"pone.0234344.ref011","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.4319\/lo.1992.37.7.1447","article-title":"Isotope fractionation associated with ammonium uptake by a marine bacterium","volume":"37","author":"MP Hoch","year":"1992","journal-title":"Limnol Oceanogr"},{"key":"pone.0234344.ref012","doi-asserted-by":"crossref","first-page":"257","DOI":"10.2527\/1995.731257x","article-title":"Fractionation of nitrogen isotopes by mixed ruminal bacteria","volume":"73","author":"MA Wattiaux","year":"1995","journal-title":"J Anim Sci"},{"key":"pone.0234344.ref013","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1264\/jsme2.ME18082","article-title":"Control of the nitrogen isotope composition of the fungal biomass: evidence of microbial nitrogen use efficiency","volume":"34","author":"K Shinoda","year":"2019","journal-title":"Microbes Environ"},{"key":"pone.0234344.ref014","doi-asserted-by":"crossref","first-page":"3182","DOI":"10.3168\/jds.2014-8215","article-title":"Evaluation of between-cow variation in milk urea and rumen ammonia nitrogen concentrations and the association with nitrogen utilization and diet digestibility in lactating cows","volume":"98","author":"P Huhtanen","year":"2015","journal-title":"J Dairy Sci"},{"key":"pone.0234344.ref015","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1017\/S1751731115002025","article-title":"Relationship between efficiency of nitrogen utilization and isotopic nitrogen fractionation in dairy cows: contribution of digestion v. metabolism?","volume":"10","author":"G Cantalapiedra-Hijar","year":"2016","journal-title":"Animal"},{"key":"pone.0234344.ref016","doi-asserted-by":"crossref","first-page":"640","DOI":"10.3920\/978-90-8686-292-4","volume-title":"INRA feeding system for ruminants","author":"INRA","year":"2018"},{"key":"pone.0234344.ref017","unstructured":"AOAC. Official methods of analysis. Assoc Anal Chem. 1990."},{"key":"pone.0234344.ref018","doi-asserted-by":"crossref","first-page":"3583","DOI":"10.3168\/jds.S0022-0302(91)78551-2","article-title":"Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition","volume":"74","author":"PJ Van Soest","year":"1991","journal-title":"J Dairy Sci"},{"key":"pone.0234344.ref019","first-page":"83","article-title":"Resistant starch determination adapted to product containing high level of resistant starch","volume":"15","author":"N Faisant","year":"1995","journal-title":"Sci Aliments"},{"key":"pone.0234344.ref020","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1051\/animres:19880403","article-title":"Mise au point d'une m\u00e9thode de laboratoire de pr\u00e9vision de la d\u00e9gradabilit\u00e9 des prot\u00e9ines alimentaires des aliments concentr\u00e9s dans le rumen","volume":"37","author":"J Aufrere","year":"1988","journal-title":"Ann Zootech"},{"key":"pone.0234344.ref021","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1017\/S002185960800779X","article-title":"CompoCow: a predictive model to estimate variations in body composition and the energy requirements of cull cows during finishing","volume":"146","author":"F Garcia","year":"2008","journal-title":"J Agric Sci"},{"key":"pone.0234344.ref022","first-page":"41","article-title":"Estimation de la composition de la carcasse des taurillons \u00e0 partir de la composition de la 6\u00e8me c\u00f4te","volume":"22","author":"J Robelin","year":"1975","journal-title":"Bull Tech CRZV Theix INRA"},{"key":"pone.0234344.ref023","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1079\/BJN19900143","article-title":"Lipid metabolism of liquid-associated and solid-adherent bacteria in rumen contents of dairy cows offered lipid-supplemented diets","volume":"63","author":"D Bauchart","year":"1990","journal-title":"Br J Nutr"},{"key":"pone.0234344.ref024","first-page":"306","volume-title":"Microdiffusion analysis and volumetric error","author":"EJ Conway","year":"1947"},{"key":"pone.0234344.ref025","doi-asserted-by":"crossref","first-page":"2962","DOI":"10.2527\/1994.72112962x","article-title":"Isolation and characteristics of the protozoal and bacterial fractions from bovine ruminal contents","volume":"72","author":"C Martin","year":"1994","journal-title":"J Anim Sci"},{"key":"pone.0234344.ref026","doi-asserted-by":"crossref","first-page":"905","DOI":"10.2527\/jas.2008-1360","article-title":"Turnover of carbon, nitrogen, and sulfur in bovine longissimus dorsi and psoas major muscles: Implications for isotopic authentication of meat","volume":"87","author":"B Bahar","year":"2009","journal-title":"J Anim Sci"},{"key":"pone.0234344.ref027","doi-asserted-by":"crossref","first-page":"74","DOI":"10.3769\/radioisotopes.36.2_74","article-title":"Variations of natural 15N abundances in the tissues and digesta of domestic animals","volume":"36","author":"M Sutoh","year":"1987","journal-title":"Radioisotopes"},{"key":"pone.0234344.ref028","doi-asserted-by":"crossref","first-page":"495","DOI":"10.3168\/jds.S0022-0302(82)82223-6","article-title":"A dairy cow body condition scoring system and its relationship to selected production characteristics","volume":"65","author":"E Wildman","year":"1982","journal-title":"J Dairy Sci"},{"key":"pone.0234344.ref029","first-page":"476","volume-title":"Nutritional ecology of the ruminant","author":"PJ Van Soest","year":"2018"},{"key":"pone.0234344.ref030","doi-asserted-by":"crossref","first-page":"3551","DOI":"10.2527\/1992.70113551x","article-title":"A net carbohydrate and protein system for evaluating cattle diets: I. Ruminal fermentation","volume":"70","author":"JB Russell","year":"1992","journal-title":"J Anim Sci"},{"key":"pone.0234344.ref031","doi-asserted-by":"crossref","first-page":"3013","DOI":"10.3168\/jds.S0022-0302(02)74387-7","article-title":"Effect of dietary fiber on endogenous nitrogen flows in lactating dairy cows","volume":"85","author":"D Ouellet","year":"2002","journal-title":"J Dairy Sci"},{"key":"pone.0234344.ref032","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1007\/BF01617819","article-title":"Amino acid utilization and isotope discrimination of amino nitrogen in nitrogen metabolism of rat liverin vivo","volume":"36","author":"H Sick","year":"1997","journal-title":"Z Ernahrungswiss"},{"key":"pone.0234344.ref033","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.3168\/jds.S0022-0302(84)81410-1","article-title":"Protein-energy interrelationships in dairy cows","volume":"67","author":"J Oldham","year":"1984","journal-title":"J Dairy Sci"},{"key":"pone.0234344.ref034","doi-asserted-by":"crossref","first-page":"5151","DOI":"10.3168\/jds.2014-8019","article-title":"Diets rich in starch increase the posthepatic availability of amino acids in dairy cows fed diets at low and normal protein levels","volume":"97","author":"G Cantalapiedra-Hijar","year":"2014","journal-title":"J Dairy Sci"},{"key":"pone.0234344.ref035","doi-asserted-by":"crossref","first-page":"5785","DOI":"10.2527\/jas.2012-5378","article-title":"Nitrogen isotopic fractionation can be used to predict nitrogen-use efficiency in dairy cows fed temperate pasture","volume":"91","author":"L Cheng","year":"2013","journal-title":"J Anim Sci"},{"key":"pone.0234344.ref036","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1007\/BF01692880","article-title":"Postprandial variations in the activity of polysaccharide-degrading enzymes of fluid- and particle-associated ruminal microbial populations","volume":"27","author":"C Martin","year":"1993","journal-title":"Curr Microbiol"},{"key":"pone.0234344.ref037","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1051\/rnd:2001122","article-title":"Fibrolytic activities and cellulolytic bacterial community structure in the solid and liquid phases of rumen contents","volume":"41","author":"B Michalet-Doreau","year":"2001","journal-title":"Reprod Nutr Dev"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0234344","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,6,5]],"date-time":"2020-06-05T17:39:42Z","timestamp":1591378782000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0234344"}},"subtitle":[],"editor":[{"given":"Marcio de Souza","family":"Duarte","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,6,5]]},"references-count":37,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2020,6,5]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0234344","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,5]]}}}