{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T13:47:15Z","timestamp":1789652835315,"version":"4.0.0"},"reference-count":28,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2020,8,21]],"date-time":"2020-08-21T00:00:00Z","timestamp":1597968000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0237791","type":"journal-article","created":{"date-parts":[[2020,8,21]],"date-time":"2020-08-21T17:48:12Z","timestamp":1598032092000},"page":"e0237791","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":12,"title":["The lack of K13-propeller mutations associated with artemisinin resistance in Plasmodium falciparum in Democratic Republic of Congo (DRC)"],"prefix":"10.1371","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8703-1269","authenticated-orcid":true,"given":"Doudou Malekita","family":"Yobi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadine Kalenda","family":"Kayiba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dieudonn\u00e9 Makaba","family":"Mvumbi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raphael","family":"Boreux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastien","family":"Bontems","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pius Zakayi","family":"Kabututu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"De Mol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niko","family":"Speybroeck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georges Lelo","family":"Mvumbi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Pierre","family":"Hayette","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2020,8,21]]},"reference":[{"issue":"10","key":"pone.0237791.ref001","doi-asserted-by":"crossref","first-page":"1503","DOI":"10.1111\/j.1365-3156.2006.01710.x","article-title":"Artesunate+amodiaquine and artesunate+sulphadoxine-pyrimethamine for treatment of uncomplicated malaria in Democratic Republic of Congo: a clinical trial with determination of sulphadoxine and pyrimethamine-resistant haplotypes","volume":"11","author":"TD Swarthout","year":"2006","journal-title":"Trop Med Int Health"},{"key":"pone.0237791.ref002","doi-asserted-by":"crossref","first-page":"153","DOI":"10.4269\/ajtmh.2007.77.153","article-title":"Responding to the challenge of antimalarial drug resistance by routine monitoring to update national malaria treatment policies","volume":"77","author":"LS Vestergaard","year":"2007","journal-title":"Am J Trop Med Hyg"},{"issue":"3_Suppl","key":"pone.0237791.ref003","doi-asserted-by":"crossref","first-page":"57","DOI":"10.4269\/ajtmh.15-0007","article-title":"Antimalarial Drug Resistance: Literature Review and Activities and Findings of the ICEMR Network","volume":"93","author":"L Cui","year":"2015","journal-title":"Am J Trop Med Hyg"},{"issue":"5","key":"pone.0237791.ref004","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1056\/NEJMoa1314981","article-title":"Spread of artemisinin resistance in Plasmodium falciparum malaria","volume":"371","author":"EA Ashley","year":"2014","journal-title":"N Engl J Med"},{"issue":"7481","key":"pone.0237791.ref005","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1038\/nature12876","article-title":"A molecular marker of artemisinin-resistant Plasmodium falciparum malaria","volume":"505","author":"F Ariey","year":"2014","journal-title":"Nature"},{"issue":"25","key":"pone.0237791.ref006","doi-asserted-by":"crossref","first-page":"2453","DOI":"10.1056\/NEJMoa1513137","article-title":"A Worldwide Map of Plasmodium falciparum K13-Propeller Polymorphisms","volume":"374","author":"D M\u00e9nard","year":"2016","journal-title":"N Engl J Med"},{"key":"pone.0237791.ref007","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12916-018-1207-3","article-title":"Association of mutations in the Plasmodium falciparum (Pf3D7_1343700) with parasite clearance rates after artemisinin-based treatments\u2013a WWARN individual patient data meta-analysis","volume":"17","author":"WWARN K13 Genotype-Phenotype Study Group","year":"2019","journal-title":"BMC Medicine"},{"key":"pone.0237791.ref008","unstructured":"World Health Organization\/Global Malaria programme. Status report on artemisinin resistance and ACT efficacy (August 2018). https:\/\/www.who.int\/malaria\/publications\/atoz\/artemisinin-resistance-august2018\/en\/. Accessed April 2019."},{"key":"pone.0237791.ref009","first-page":"1352","article-title":"K13 Propeller Polymorphisms in Plasmodium falciparum Parasites from Sub-Saharan Africa: Brief repport","volume":"211","author":"E Kamau","year":"2015","journal-title":"J Infect Dis"},{"key":"pone.0237791.ref010","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1051\/parasite\/2016028","article-title":"Lack of artemisinin resistance in Plasmodium falciparum in northwest Benin after 10 years of use of artemisinin-based combination therapy","volume":"23","author":"A Ogouy\u00e8mi-Hounto","year":"2016","journal-title":"Parasite"},{"issue":"5","key":"pone.0237791.ref011","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1093\/infdis\/jiu467","article-title":"Absence of Putative Artemisinin Resistance Mutations Among Plasmodium falciparum in Sub-Saharan Africa: A Molecular Epidemiologic Study","volume":"211","author":"S Taylor","year":"2015","journal-title":"J Infect Dis"},{"issue":"5","key":"pone.0237791.ref012","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.4269\/ajtmh.16-0483","article-title":"Artemisinin Resistance\u2013Associated K13 Polymorphisms of Plasmodium falciparum in Southern Rwanda, 2010\u20132015","volume":"95","author":"C Tacoli","year":"2016","journal-title":"Am J Trop Med Hyg"},{"key":"pone.0237791.ref013","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1186\/s12936-018-2524-x","article-title":"Efficacy and safety of artemisinin-based combination therapy, and molecular markers for artemisinin and piperaquine resistance in Mainland Tanzania","volume":"17","author":"M Kakolwa","year":"2018","journal-title":"Malar J"},{"issue":"4","key":"pone.0237791.ref014","doi-asserted-by":"crossref","first-page":"718","DOI":"10.3201\/eid2404.170141","article-title":"Artemisinin-Resistant Plasmodium falciparum with High Survival Rates, Uganda, 2014\u20132016","volume":"24","author":"M Ikeda","year":"2018","journal-title":"Emerg Infect Dis"},{"issue":"6895","key":"pone.0237791.ref015","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1038\/nature00813","article-title":"Genetic diversity and chloroquine selective sweeps in Plasmodium falciparum","volume":"418","author":"JC Wootton","year":"2002","journal-title":"Nature"},{"issue":"12","key":"pone.0237791.ref016","doi-asserted-by":"crossref","first-page":"1980","DOI":"10.1093\/infdis\/jir664","article-title":"Limited geographical origin and global spread of sulfadoxine-resistant dhps alleles in Plasmodium falciparum populations","volume":"204","author":"T Mita","year":"2011","journal-title":"J Infect Dis"},{"issue":"6","key":"pone.0237791.ref017","doi-asserted-by":"crossref","first-page":"e0179142","DOI":"10.1371\/journal.pone.0179142","article-title":"Molecular surveillance of Plasmodium falciparum resistance to artemisinin-based combination therapies in the Democratic Republic of Congo","volume":"12","author":"DM Mvumbi","year":"2017","journal-title":"PLOS ONE"},{"issue":"7","key":"pone.0237791.ref018","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1111\/j.1469-0691.2010.03344.x","article-title":"Validation of a four-primer real-time PCR as a diagnostic tool for single and mixed Plasmodium infections","volume":"17","author":"L Cnops","year":"2011","journal-title":"Clin Microbiol Infect"},{"issue":"1","key":"pone.0237791.ref019","doi-asserted-by":"crossref","first-page":"1980","DOI":"10.1186\/s12936-016-1622-x","article-title":"Insight into k13-propeller gene polymorphism and ex vivo DHA-response profiles from Cameroonian isolates","volume":"15","author":"S Menard","year":"2016","journal-title":"Malar J"},{"key":"pone.0237791.ref020","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1186\/s12879-018-3453-6","article-title":"No polymorphisms in K13-propeller gene associated with artemisinin resistance in Plasmodium falciparum isolated from Brazzaville, Republic of Congo","volume":"18","author":"P Mayengue","year":"2018","journal-title":"BMC Infect Dis"},{"key":"pone.0237791.ref021","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1186\/s12936-015-1018-3","article-title":"Evaluation of artemether-lumefantrine efficacy in the treatment of uncomplicated malaria and its association with pfmdr1, pfatpase6 and K13-propeller polymorphisms in Luanda, Angola","volume":"14","author":"K Kiaco","year":"2015","journal-title":"Malar J"},{"issue":"1","key":"pone.0237791.ref022","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1186\/1475-2875-13-431","article-title":"Mutations in Plasmodium falciparum K13 propeller gene from Bangladesh (2009\u20132013)","volume":"13","author":"A Mohon","year":"2014","journal-title":"Malar J"},{"key":"pone.0237791.ref023","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.drup.2004.08.003","article-title":"Withdrawing antimalarial drugs: impact on parasite resistance and implications for malaria treatment policies","volume":"7","author":"M Laufer","year":"2004","journal-title":"Drug Resist Updates"},{"issue":"4","key":"pone.0237791.ref024","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/S1473-3099(02)00239-6","article-title":"Epidemiology of drug-resistant malaria","volume":"2","author":"C Wongsrichanalai","year":"2002","journal-title":"Lancet Infect Dis"},{"issue":"9","key":"pone.0237791.ref025","doi-asserted-by":"crossref","first-page":"e0221895","DOI":"10.1371\/journal.pone.0221895","article-title":"K-13 propeller gene polymorphisms isolated between 2014 and 2017 from Cameroonian Plasmodium falciparum malaria patients","volume":"14","author":"CE Eboumbou Moukoko","year":"2019","journal-title":"PLOS ONE"},{"issue":"3","key":"pone.0237791.ref026","doi-asserted-by":"crossref","first-page":"490","DOI":"10.3201\/eid2103.140898","article-title":"Novel Mutations in K13 Propeller Gene of artemisinin-resistant Plasmodium falciparum","volume":"21","author":"R Isozumi","year":"2015","journal-title":"Emerg Infect Dis"},{"issue":"6220","key":"pone.0237791.ref027","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1126\/science.1260867","article-title":"K13-propeller mutations confer artemisinin resistance in Plasmodium falciparum clinical isolates","volume":"347","author":"J Straimer","year":"2014","journal-title":"Science"},{"key":"pone.0237791.ref028","unstructured":"President's Malaria Initiative: Democratic Republic of the Congo\u2014Malaria Operational Plan FY2018. reliefweb.int\/report\/democratic-republic-congo\/presidents-malaria-initiative-democratic-republic-congo-malaria-0. Accessed May 2020."}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0237791","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,21]],"date-time":"2020-08-21T17:48:35Z","timestamp":1598032115000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0237791"}},"subtitle":[],"editor":[{"given":"Md Atique","family":"Ahmed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,8,21]]},"references-count":28,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2020,8,21]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0237791","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,21]]}}}