{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T20:11:20Z","timestamp":1762459880857,"version":"3.37.3"},"reference-count":27,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2020,8,21]],"date-time":"2020-08-21T00:00:00Z","timestamp":1597968000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0237985","type":"journal-article","created":{"date-parts":[[2020,8,21]],"date-time":"2020-08-21T17:39:11Z","timestamp":1598031551000},"page":"e0237985","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["Human cultured epidermis accelerates wound healing regardless of its viability in a diabetic mouse model"],"prefix":"10.1371","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0024-6807","authenticated-orcid":true,"given":"Michiharu","family":"Sakamoto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuichi","family":"Ogino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihiro","family":"Shimizu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masukazu","family":"Inoie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sunghee","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroki","family":"Yamanaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Itaru","family":"Tsuge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susumu","family":"Saito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0712-3172","authenticated-orcid":true,"given":"Naoki","family":"Morimoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2020,8,21]]},"reference":[{"key":"pone.0237985.ref001","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1111\/j.1742-481X.2012.01083.x","article-title":"Keratinocytes in the treatment of severe burn injury: an update","volume":"10","author":"L Lootens","year":"2013","journal-title":"Int Wound J"},{"key":"pone.0237985.ref002","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1016\/j.burns.2016.01.019","article-title":"Application of the cultured epidermal autograft \u201cJACE\u00ae\u201d for treatment of severe burns: Results of a 6-year multicenter surveillance in Japan","volume":"42","author":"H Matsumura","year":"2016","journal-title":"Burns"},{"key":"pone.0237985.ref003","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1097\/00004630-199111000-00008","article-title":"Cultured epithelial autografts: three years of clinical experience with eighteen patients","volume":"12","author":"PA Clugston","year":"1991","journal-title":"J Burn Care Rehabil"},{"key":"pone.0237985.ref004","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1016\/S0140-6736(83)90392-6","article-title":"Grafting of burn patients with allografts of cultured epidermal cells","volume":"2","author":"JM Hefton","year":"1983","journal-title":"Lancet"},{"key":"pone.0237985.ref005","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1097\/00005373-198611000-00001","article-title":"Grafting of cultured allogeneic epidermis on second- and third-degree burn wounds on 26 patients","volume":"26","author":"MR Madden","year":"1986","journal-title":"J Trauma"},{"key":"pone.0237985.ref006","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/0305-4179(89)90007-7","article-title":"Multicentre experience in the treatment of burns with autologous and allogenic cultured epithelium, fresh or preserved in a frozen state","volume":"15","author":"M De Luca","year":"1989","journal-title":"Burns"},{"key":"pone.0237985.ref007","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/0305-4179(90)90038-X","article-title":"Allografted keratinocytes used to accelerate the treatment of burn wounds are replaced by recipient cells","volume":"16","author":"AE van der Merwe","year":"1990","journal-title":"Burns"},{"issue":"Suppl 1","key":"pone.0237985.ref008","doi-asserted-by":"crossref","first-page":"S16","DOI":"10.1016\/0305-4179(92)90105-4","article-title":"Permanent coverage of full skin thickness burns with autologous cultured epidermis and reepithelialization of partial skin thickness lesions induced by allogeneic cultured epidermis: a multicentre study in the treatment of children","volume":"18","author":"M De Luca","year":"1992","journal-title":"Burns"},{"key":"pone.0237985.ref009","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1016\/0190-9622(93)70278-2","article-title":"Randomized trial comparing cryopreserved cultured epidermal allografts with hydrocolloid dressings in healing chronic venous ulcers","volume":"29","author":"RG Teepe","year":"1993","journal-title":"J Am Acad Dermatol"},{"key":"pone.0237985.ref010","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1001\/archderm.1993.01680280067013","article-title":"A randomized single-blind controlled study of cultured epidermal allografts in the treatment of split-thickness skin graft donor sites","volume":"129","author":"TJ Phillips","year":"1993","journal-title":"Arch Dermatol"},{"key":"pone.0237985.ref011","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1097\/00006534-199608000-00011","article-title":"Controlled clinical study of skin donor sites and deep partial-thickness burns treated with cultured epidermal allografts","volume":"98","author":"MT Rivas-Torres","year":"1996","journal-title":"Plast Reconstr Surg"},{"key":"pone.0237985.ref012","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1097\/00004630-199711000-00013","article-title":"Cultured allogeneic keratinocyte sheets accelerate healing compared to Op-site treatment of donor sites in burns","volume":"18","author":"LA Duinslaeger","year":"1997","journal-title":"J Burn Care Rehabil"},{"key":"pone.0237985.ref013","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1159\/000247628","article-title":"Repeated cultured epidermal allografts in the treatment of chronic leg ulcers of various origins","volume":"183","author":"H Beele","year":"1991","journal-title":"Dermatologica"},{"key":"pone.0237985.ref014","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1046\/j.1365-2133.1998.02364.x","article-title":"Treatment of venous leg ulcers with cryopreserved cultured allogeneic keratinocytes: a prospective open controlled study","volume":"139","author":"C Lindgren","year":"1998","journal-title":"Br J Dermatol"},{"key":"pone.0237985.ref015","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1111\/j.1365-2133.1987.tb07491.x","article-title":"Treatment of chronic venous ulcers with sheets of cultured allogenic keratinocytes","volume":"117","author":"IM Leigh","year":"1987","journal-title":"Br J Dermatol"},{"key":"pone.0237985.ref016","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1007\/s004410051319","article-title":"Frozen cultured sheets of human epidermal keratinocytes enhance healing of full-thickness wounds in mice","volume":"296","author":"E Tamariz","year":"1999","journal-title":"Cell Tissue Res"},{"key":"pone.0237985.ref017","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/0305-4179(90)90197-5","article-title":"Use of cultured human epidermal keratinocytes for allografting burns and conditions for temporary banking of the cultured allografts","volume":"16","author":"J Bol\u00edvar-Flores","year":"1990","journal-title":"Burns"},{"key":"pone.0237985.ref018","doi-asserted-by":"crossref","first-page":"5665","DOI":"10.1073\/pnas.76.11.5665","article-title":"Growth of cultured human epidermal cells into multiple epithelia suitable for grafting","volume":"76","author":"H Green","year":"1979","journal-title":"Proc Natl Acad Sci U S A"},{"key":"pone.0237985.ref019","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/S0092-8674(75)80001-8","article-title":"Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells","volume":"6","author":"JG Rheinwald","year":"1975","journal-title":"Cell"},{"key":"pone.0237985.ref020","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1136\/bmj.298.6678.917","article-title":"Survival of cultured allogeneic keratinocytes transplanted to deep dermal bed assessed with probe specific for Y chromosome","volume":"298","author":"A Brain","year":"1989","journal-title":"BMJ"},{"key":"pone.0237985.ref021","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1093\/ilar.41.4.187","article-title":"Mechanisms of cryoinjury in living cells","volume":"41","author":"D Gao","year":"2000","journal-title":"ILAR J"},{"key":"pone.0237985.ref022","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1186\/s12896-016-0315-4","article-title":"Cryopreservation of dermal fibroblasts and keratinocytes in hydroxyethyl starch-based cryoprotectants","volume":"16","author":"Y Naaldijk","year":"2016","journal-title":"BMC Biotechnol"},{"key":"pone.0237985.ref023","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/B978-0-12-411604-7.00012-X","volume-title":"Biochemical Ecotoxicology. Principles and Methods","author":"F Gagn\u00e9","year":"2014"},{"key":"pone.0237985.ref024","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1111\/j.1524-475X.2010.00608.x","article-title":"Delayed wound healing in diabetic (db\/db) mice with pseudomonas aeruginosa biofilm challenge: a model for the study of chronic wounds","volume":"18","author":"G Zhao","year":"2011","journal-title":"Wound Repair Regen"},{"key":"pone.0237985.ref025","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1002\/term.1492","article-title":"Evaluation of a novel collagen-gelatin scaffold for achieving the sustained release of basic fibroblast growth factor in a diabetic mouse model","volume":"8","author":"N Kanda","year":"2014","journal-title":"J Tissue Eng Regen Med"},{"key":"pone.0237985.ref026","doi-asserted-by":"crossref","first-page":"907209","DOI":"10.1155\/2013\/907209","article-title":"Wound-healing potential of cultured epidermal sheets is unaltered after lyophilization: a preclinical study in comparison to cryopreserved CES","volume":"2013","author":"H Jang","year":"2013","journal-title":"Biomed Res Int"},{"key":"pone.0237985.ref027","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1097\/00007890-198707000-00022","article-title":"Prolonged survival of cultured keratinocyte allografts in the nonimmunosuppressed mouse","volume":"44","author":"EJ Hammond","year":"1987","journal-title":"Transplantation"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0237985","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,21]],"date-time":"2020-08-21T17:39:19Z","timestamp":1598031559000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0237985"}},"subtitle":[],"editor":[{"given":"Feng","family":"Zhao","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2020,8,21]]},"references-count":27,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2020,8,21]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0237985","relation":{},"ISSN":["1932-6203"],"issn-type":[{"type":"electronic","value":"1932-6203"}],"subject":[],"published":{"date-parts":[[2020,8,21]]}}}