{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T14:49:42Z","timestamp":1781534982958,"version":"3.54.5"},"reference-count":12,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2020,11,17]],"date-time":"2020-11-17T00:00:00Z","timestamp":1605571200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    We previously identified a diazaborine series with potential for development as a new tuberculosis drug. This series has activity\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    and targets cell wall biosynthesis via inhibition of InhA. The overall aim of this study was to determine whether InhA inhibitors have activity against non-replicating\n                    <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                    . We tested the ability of two molecules of the diazaborine series to kill non-replicating\n                    <jats:italic>M<\/jats:italic>\n                    .\n                    <jats:italic>tuberculosis<\/jats:italic>\n                    in the nutrient starvation model; both molecules were bactericidal, reducing viability by &gt;3 logs in 21 days. Activity showed similar kill rates to other InhA inhibitors (isoniazid and NITD-916). We conclude that inhibition of InhA is bactericidal against nutrient-starved non-replicating\n                    <jats:italic>M<\/jats:italic>\n                    .\n                    <jats:italic>tuberculosis<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0239354","type":"journal-article","created":{"date-parts":[[2020,11,17]],"date-time":"2020-11-17T13:32:22Z","timestamp":1605619942000},"page":"e0239354","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":12,"title":["InhA inhibitors have activity against non-replicating Mycobacterium tuberculosis"],"prefix":"10.1371","volume":"15","author":[{"given":"Lindsay","family":"Flint","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron","family":"Korkegian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7507-0423","authenticated-orcid":true,"given":"Tanya","family":"Parish","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2020,11,17]]},"reference":[{"issue":"4","key":"pone.0239354.ref001","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1099\/mic.0.000601","article-title":"Microbe profile: Mycobacterium tuberculosis: Humanity\u2019s deadly microbial foe","volume":"164","author":"SV Gordon","year":"2018","journal-title":"Microbiol"},{"key":"pone.0239354.ref002","unstructured":"World Health Organization. Global tuberculosis report 2019. 2020."},{"issue":"5","key":"pone.0239354.ref003","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1099\/mic.0.000760","article-title":"The relevance of persisters in tuberculosis drug discovery","volume":"165","author":"S Mandal","year":"2019","journal-title":"Microbiol"},{"issue":"3","key":"pone.0239354.ref004","doi-asserted-by":"crossref","first-page":"e201800025","DOI":"10.26508\/lsa.201800025","article-title":"Discovery of a cofactor-independent inhibitor of Mycobacterium tuberculosis InhA","volume":"1","author":"Y Xia","year":"2018","journal-title":"Life Sci Alliance"},{"issue":"4","key":"pone.0239354.ref005","doi-asserted-by":"crossref","DOI":"10.1128\/AAC.02071-18","article-title":"Efficacy and Improved resistance potential of a cofactor-independent InhA inhibitor of Mycobacterium tuberculosis in the C3HeB\/FeJ Mouse Model","volume":"63","author":"GT Robertson","year":"2019","journal-title":"Antimicrob Agents Chemother"},{"issue":"14","key":"pone.0239354.ref006","doi-asserted-by":"crossref","DOI":"10.1128\/JB.00166-10","article-title":"Variation among genome sequences of H37Rv strains of Mycobacterium tuberculosis from multiple laboratories","volume":"192","author":"TR Ioerger","year":"2010","journal-title":"J Bacteriol"},{"key":"pone.0239354.ref007","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1007\/978-1-4939-2450-9_16","article-title":"Determination of compound kill kinetics against Mycobacterium tuberculosis","volume":"1285","author":"J Early","year":"2015","journal-title":"Methods Mol Biol"},{"issue":"3","key":"pone.0239354.ref008","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1046\/j.1365-2958.2002.02779.x","article-title":"Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling","volume":"43","author":"JC Betts","year":"2002","journal-title":"Mol Microbiol"},{"issue":"10","key":"pone.0239354.ref009","doi-asserted-by":"crossref","first-page":"e0198059","DOI":"10.1371\/journal.pone.0198059","article-title":"A class of hydrazones are active against nonreplicating Mycobacterium tuberculosis","volume":"13","author":"SA Bonnett","year":"2018","journal-title":"PLoS One"},{"issue":"10","key":"pone.0239354.ref010","doi-asserted-by":"crossref","first-page":"6521","DOI":"10.1128\/AAC.00803-15","article-title":"Rapid, semiquantitative assay to discriminate among compounds with activity against replicating or nonreplicating Mycobacterium tuberculosis","volume":"59","author":"B Gold","year":"2015","journal-title":"Antimicrob Agents Chemother"},{"issue":"9","key":"pone.0239354.ref011","doi-asserted-by":"crossref","first-page":"2054","DOI":"10.1128\/AAC.38.9.2054","article-title":"Metronidazole is bactericidal to dormant cells of Mycobacterium tuberculosis","volume":"38","author":"LG Wayne","year":"1994","journal-title":"Antimicrob Agents Chemother"},{"issue":"269","key":"pone.0239354.ref012","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.3010597","article-title":"Direct inhibitors of InhA are active against Mycobacterium tuberculosis","volume":"7","author":"UH Manjunatha","year":"2015","journal-title":"Sci Transl Med"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0239354","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,17]],"date-time":"2020-11-17T13:32:46Z","timestamp":1605619966000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0239354"}},"subtitle":[],"editor":[{"given":"Olivier","family":"Neyrolles","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,11,17]]},"references-count":12,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2020,11,17]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0239354","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.08.19.257782","asserted-by":"object"}]},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,17]]}}}