{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T04:12:09Z","timestamp":1747195929656,"version":"3.40.5"},"reference-count":33,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2020,11,6]],"date-time":"2020-11-06T00:00:00Z","timestamp":1604620800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Yunnan Applied Basic Research Projects","award":["2019FB018"],"award-info":[{"award-number":["2019FB018"]}]},{"name":"Yunnan Applied Basic Research Projects","award":["2018ZF006"],"award-info":[{"award-number":["2018ZF006"]}]},{"name":"National Natural Sciences Foundations of China","award":["31700153"],"award-info":[{"award-number":["31700153"]}]},{"name":"Fundamental Research Funds for the Central Universities and PUMC Youth Fund","award":["3332019004"],"award-info":[{"award-number":["3332019004"]}]},{"name":"Medical Reserve Talents of Yunnan Province Health and Family Planning","award":["H-2017034"],"award-info":[{"award-number":["H-2017034"]}]},{"name":"Top young talents of Yunnan province ten thousand talents plan"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Coxsackievirus A16 (CV-A16) is one of the viruses that is most frequently associated with hand-foot-and-mouth disease (HFMD). Previous studies have shown that CV-A16 infections are mostly self-limiting, but in recent years, it has been gradually found that CV-A16 infections can also induce neurological complications and eventually cause death in children with HFMD. Moreover, no curative drugs or preventative vaccines have been developed for CV-A16 infection. Therefore, it is particularly important to investigate the mechanism of CV-A16 infection-induced neuropathy. In the current study, transcriptome sequencing technology was used to identify changes in the transcriptome of SH-SY5Y cells infected with CV-A16, which might hide the mechanism of CV-A16-induced neuropathology. The transcriptome profiling showed that 82,406,974, 108,652,260 and 97,753,565 clean reads were obtained in the Control, CV-A16-12 h and CV-A16-24 h groups, respectively. And it was further detected that a total of 136 and 161 differentially expressed genes in CV-A16-12 h and CV-A16-24 h groups, respectively, when compared with Control group. Then, to explore the mechanism of CV-A16 infection, we focused on the common differentially expressed genes at different time points of CV-A16 infection and found that there were 34 differentially expressed genes based on which clustering analysis and functional category enrichment analysis were performed. The results indicated that changes in oxidation levels were particularly evident in the GO term analysis, while only the \u201cGonadotropin-releasing hormone receptor pathway\u201d was enriched in the KEGG pathway analysis, which might be closely related to the neurotoxicity caused by CV-A16 infection. Meanwhile, the ID2 closely related to nervous system has been demonstrated to be increased during CV-A16 infection. Additionally, the data on differentially expressed non-protein-coding genes of different types within the transcriptome sequencing results were analyzed, and it was speculated that these dysregulated non-protein-coding genes played a pivotal role in CV-A16 infection. Ultimately, qRT-PCR was utilized to validate the transcriptome sequencing findings, and the results of qRT-PCR were in agreement with the transcriptome sequencing data. In conclusion, transcriptome profiling was carried out to analyze response of SH-SY5Y cells to CV-A16 infection. And our findings provide important information to elucidate the possible molecular mechanisms which were linked to the neuropathogenesis of CV-A16 infection.<\/jats:p>","DOI":"10.1371\/journal.pone.0241174","type":"journal-article","created":{"date-parts":[[2020,11,6]],"date-time":"2020-11-06T18:33:22Z","timestamp":1604687602000},"page":"e0241174","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Comprehensive analysis of differential expression profiles via transcriptome sequencing in SH-SY5Y cells infected with CV-A16"],"prefix":"10.1371","volume":"15","author":[{"given":"Yajie","family":"Hu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shenglan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danxiong","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingmei","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mudong","family":"Wen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9971-0528","authenticated-orcid":true,"given":"Yunhui","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2020,11,6]]},"reference":[{"issue":"3","key":"pone.0241174.ref001","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1007\/s10096-018-3206-x","article-title":"Hand, foot and mouth disease: current knowledge on clinical manifestations, epidemiology, aetiology and prevention","volume":"37","author":"S Esposito","year":"2018","journal-title":"Eur J Clin Microbiol Infect Dis"},{"volume-title":"Hand Foot And Mouth Disease","year":"2019","author":"AM Guerra","key":"pone.0241174.ref002"},{"key":"pone.0241174.ref003","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.ijid.2017.09.020","article-title":"Risk factors for neurogenic pulmonary edema in patients with severe hand, foot, and mouth disease: A meta-analysis","volume":"65","author":"L Peng","year":"2017","journal-title":"Int J Infect Dis"},{"issue":"5","key":"pone.0241174.ref004","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1111\/bpa.12279","article-title":"Understanding Enterovirus 71 Neuropathogenesis and Its Impact on Other Neurotropic Enteroviruses","volume":"25","author":"KC Ong","year":"2015","journal-title":"Brain Pathol"},{"issue":"10","key":"pone.0241174.ref005","doi-asserted-by":"crossref","first-page":"395","DOI":"10.3345\/kjp.2016.59.10.395","article-title":"Enterovirus 71 infection and neurological complications","volume":"59","author":"KY Lee","year":"2016","journal-title":"Korean J Pediatr"},{"issue":"5","key":"pone.0241174.ref006","first-page":"599","article-title":"EV71 vaccine, a new tool to control outbreaks of hand, foot and mouth disease (HFMD). 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