{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T06:48:46Z","timestamp":1776494926217,"version":"3.51.2"},"reference-count":21,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2020,10,30]],"date-time":"2020-10-30T00:00:00Z","timestamp":1604016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003825","name":"Magyar Tudom\u00e1nyos Akad\u00e9mia","doi-asserted-by":"publisher","award":["LP2012-22"],"award-info":[{"award-number":["LP2012-22"]}],"id":[{"id":"10.13039\/501100003825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011019","name":"Nemzeti Kutat\u00e1si Fejleszt\u00e9si \u00e9s Innov\u00e1ci\u00f3s Hivatal","doi-asserted-by":"publisher","award":["K_16 (119594)"],"award-info":[{"award-number":["K_16 (119594)"]}],"id":[{"id":"10.13039\/501100011019","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011019","name":"Nemzeti Kutat\u00e1si Fejleszt\u00e9si \u00e9s Innov\u00e1ci\u00f3s Hivatal","doi-asserted-by":"publisher","award":["FK_17 (124019)"],"award-info":[{"award-number":["FK_17 (124019)"]}],"id":[{"id":"10.13039\/501100011019","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011019","name":"Nemzeti Kutat\u00e1si Fejleszt\u00e9si \u00e9s Innov\u00e1ci\u00f3s Hivatal","doi-asserted-by":"publisher","award":["KKP19 (129751)"],"award-info":[{"award-number":["KKP19 (129751)"]}],"id":[{"id":"10.13039\/501100011019","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003825","name":"Magyar Tudom\u00e1nyos Akad\u00e9mia","doi-asserted-by":"publisher","award":["Bolyai J\u00e1nos Research Fellowship"],"award-info":[{"award-number":["Bolyai J\u00e1nos Research Fellowship"]}],"id":[{"id":"10.13039\/501100003825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003825","name":"Magyar Tudom\u00e1nyos Akad\u00e9mia","doi-asserted-by":"publisher","award":["Bolyai J\u00e1nos Research Fellowship"],"award-info":[{"award-number":["Bolyai J\u00e1nos Research Fellowship"]}],"id":[{"id":"10.13039\/501100003825","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Innov\u00e1ci\u00f3s \u00e9s Technol\u00f3giai Miniszt\u00e9rium","award":["Bolyai+ Fellowship (\u00daNKP-19-4-\u00c1TE-1)"],"award-info":[{"award-number":["Bolyai+ Fellowship (\u00daNKP-19-4-\u00c1TE-1)"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0241647","type":"journal-article","created":{"date-parts":[[2020,10,30]],"date-time":"2020-10-30T17:40:50Z","timestamp":1604079650000},"page":"e0241647","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Development of molecular assays for the rapid and cost-effective determination of fluoroquinolone, macrolide and lincosamide susceptibility of Mycoplasma synoviae isolates"],"prefix":"10.1371","volume":"15","author":[{"given":"Katinka","family":"Bek\u0151","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zsuzsa","family":"Kreizinger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9cile","family":"Yvon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Orsolya","family":"Saller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3258-172X","authenticated-orcid":true,"given":"Salvatore","family":"Catania","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anneke","family":"Feberwee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5698-5713","authenticated-orcid":true,"given":"Mikl\u00f3s","family":"Gyuranecz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2020,10,30]]},"reference":[{"key":"pone.0241647.ref001","first-page":"900","volume-title":"Diseases of poultry","author":"N Ferguson-Noel","year":"2013","edition":"13"},{"key":"pone.0241647.ref002","first-page":"89","article-title":"Immunology and prophylaxis associated with the use of a Mycoplasma gallisepticum bacterin in chickens","volume":"108","author":"D Hildebrand","year":"1985","journal-title":"Clin Vet"},{"key":"pone.0241647.ref003","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1051\/vetres:2000100","article-title":"Guidelines and recommendations for antimicrobial minimum inhibitory concentration (MIC) testing against veterinary Mycoplasma species","volume":"31","author":"PCT Hannan","year":"2000","journal-title":"Vet Res"},{"key":"pone.0241647.ref004","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1111\/j.1600-0463.2004.apm11211-1208.x","article-title":"Genetic methods for detection of antimicrobial resistance","volume":"112","author":"A Sundsfjord","year":"2004","journal-title":"APMIS"},{"key":"pone.0241647.ref005","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.vetmic.2017.11.026","article-title":"Development of molecular methods for the rapid detection of antibiotic susceptibility of Mycoplasma bovis","volume":"213","author":"KM Sulyok","year":"2018","journal-title":"Vet Microbiol"},{"key":"pone.0241647.ref006","doi-asserted-by":"crossref","first-page":"108697","DOI":"10.1016\/j.vetmic.2020.108697","article-title":"Development of molecular biological tools for the rapid determination of antibiotic susceptibility of Mycoplasma hyopneumoniae isolates","volume":"245","author":"O Felde","year":"2020","journal-title":"Vet Microbiol"},{"key":"pone.0241647.ref007","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1051\/vetres:2005046","article-title":"Persistence of Mycoplasma synoviae in hens after two enrofloxacin treatments and detection of mutations in the parC gene","volume":"37","author":"J Le Carrou","year":"2006","journal-title":"Vet Res"},{"key":"pone.0241647.ref008","doi-asserted-by":"crossref","first-page":"3072","DOI":"10.1128\/AAC.00203-13","article-title":"Molecular characterization of acquired enrofloxacin resistance in Mycoplasma synoviae field isolates","volume":"57","author":"I Lysnyansky","year":"2013","journal-title":"Antimicrob Agents Chemother"},{"key":"pone.0241647.ref009","article-title":"Mutations potentially associated with decreased susceptibility to fluoroquinolones, macrolides and lincomycin in Mycoplasma synoviae","author":"K Bek\u0151","journal-title":"Vet Microbiol"},{"key":"pone.0241647.ref010","doi-asserted-by":"crossref","first-page":"807","DOI":"10.3855\/jidc.7850","article-title":"Mutations of domain V in 23S ribosomal RNA of macrolide resistant Mycoplasma gallisepticum isolates in Egypt","volume":"10","author":"AM Ammar","year":"2016","journal-title":"J Infect Dev Ctries"},{"key":"pone.0241647.ref011","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1089\/mdr.2014.0290","article-title":"Decreased susceptibility to macrolide-lincosamide in Mycoplasma synoviae is associated with mutations in 23S ribosomal RNA","volume":"21","author":"I Lysnyansky","year":"2015","journal-title":"Microb Drug Resist"},{"key":"pone.0241647.ref012","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1186\/s12917-017-1266-2","article-title":"Antibiotic susceptibility profiles of Mycoplasma synoviae strains originating from Central and Eastern Europe","volume":"13","author":"Z Kreizinger","year":"2017","journal-title":"BMC Vet Res"},{"key":"pone.0241647.ref013","doi-asserted-by":"crossref","first-page":"e0224903","DOI":"10.1371\/journal.pone.0224903","article-title":"Evaluation of minimum inhibitory concentrations for 154 Mycoplasma synoviae isolates from Italy collected during 2012\u20132017","volume":"14","author":"S Catania","year":"2019","journal-title":"PloS ONE"},{"key":"pone.0241647.ref014","first-page":"2163","article-title":"A medium for the isolation of avian mycoplasmas","volume":"29","author":"ML Frey","year":"1968","journal-title":"Am J Vet Res"},{"key":"pone.0241647.ref015","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1637\/8469-091508-Reg.1","article-title":"The development of diagnostic real-time Taqman PCRs for the four pathogenic avian mycoplasmas","volume":"53","author":"Z Raviv","year":"2009","journal-title":"Avian Dis"},{"key":"pone.0241647.ref016","doi-asserted-by":"crossref","first-page":"804","DOI":"10.2307\/1592417","article-title":"Avian mycoplasma identification using polymerase chain reaction amplicon and restriction fragment length polymorphism analysis","volume":"39","author":"LH Lauerman","year":"1995","journal-title":"Avian Dis"},{"key":"pone.0241647.ref017","doi-asserted-by":"crossref","first-page":"e32860","DOI":"10.1371\/journal.pone.0032866","article-title":"Melt analysis of mismatch amplification mutation assays (Melt-MAMA): a functional study of a cost-effective SNP genotyping assay in bacterial models","volume":"7","author":"DN Birdsell","year":"2012","journal-title":"PLoS One"},{"key":"pone.0241647.ref018","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1093\/bioinformatics\/bts199","article-title":"Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data","volume":"28","author":"M Kearse","year":"2012","journal-title":"Bioinformatics"},{"key":"pone.0241647.ref019","article-title":"Calculator for determining the number of copies of a template","author":"A Staroscik","year":"2004","journal-title":"URI Genomics & Sequencing Center"},{"key":"pone.0241647.ref020","doi-asserted-by":"crossref","first-page":"24","DOI":"10.32607\/20758251-2016-8-2-24-34","article-title":"Mycoplasmas and their antibiotic resistance: The problems and prospects in controlling infections","volume":"8","author":"OA Chernova","year":"2016","journal-title":"Acta Naturae"},{"key":"pone.0241647.ref021","doi-asserted-by":"crossref","first-page":"11","DOI":"10.2165\/00003495-199958002-00003","article-title":"Mechanism of fluoroquinolone-resistance: an update 1994\u20131998","volume":"58","author":"LJV Piddock","year":"1999","journal-title":"Drugs"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0241647","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,30]],"date-time":"2020-10-30T17:41:07Z","timestamp":1604079667000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0241647"}},"subtitle":[],"editor":[{"given":"Iddya","family":"Karunasagar","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2020,10,30]]},"references-count":21,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2020,10,30]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0241647","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,30]]}}}