{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:01:13Z","timestamp":1787022073348,"version":"build-2736575974"},"reference-count":22,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2020,11,2]],"date-time":"2020-11-02T00:00:00Z","timestamp":1604275200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["SPP 1784"],"award-info":[{"award-number":["SPP 1784"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0241740","type":"journal-article","created":{"date-parts":[[2020,11,2]],"date-time":"2020-11-02T13:39:08Z","timestamp":1604324348000},"page":"e0241740","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":11,"title":["Detection of SARS-CoV-2 from raw patient samples by coupled high temperature reverse transcription and amplification"],"prefix":"10.1371","volume":"15","author":[{"given":"Johannes W. P.","family":"Kuiper","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timo","family":"Baade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcel","family":"Kremer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramon","family":"Kranaster","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Irmisch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus","family":"Schuchmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"Zander","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Marx","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1005-2141","authenticated-orcid":true,"given":"Christof R.","family":"Hauck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2020,11,2]]},"reference":[{"key":"pone.0241740.ref001","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1016\/S0140-6736(20)30251-8","article-title":"Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding.","volume":"395","author":"R Lu","year":"2020","journal-title":"Lancet"},{"key":"pone.0241740.ref002","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1038\/s41586-020-2012-7","article-title":"A pneumonia outbreak associated with a new coronavirus of probable bat origin","volume":"579","author":"P Zhou","year":"2020","journal-title":"Nature"},{"key":"pone.0241740.ref003","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/bs.aivir.2016.08.008","article-title":"The Nonstructural Proteins Directing Coronavirus RNA Synthesis and Processing.","volume":"96","author":"EJ Snijder","year":"2016","journal-title":"Advances in Virus Research"},{"key":"pone.0241740.ref004","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1006\/viro.1995.0009","article-title":"Equine arteritis virus subgenomic RNA transcription: UV inactivation and translation inhibition studies","volume":"213","author":"JA Den Boon","year":"1995","journal-title":"Virology"},{"key":"pone.0241740.ref005","doi-asserted-by":"crossref","first-page":"e1000772","DOI":"10.1371\/journal.ppat.1000772","article-title":"Arterivirus Nsp1 modulates the accumulation of minus-strand templates to control the relative abundance of viral mRNAs.","volume":"6","author":"DD Nedialkova","year":"2010","journal-title":"PLoS pathogens."},{"key":"pone.0241740.ref006","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.1016\/S1473-3099(14)70827-8","article-title":"Rapid point of care diagnostic tests for viral and bacterial respiratory tract infections\u2014needs, advances, and future prospects","volume":"14","author":"A Zumla","year":"2014","journal-title":"The Lancet Infectious Diseases"},{"key":"pone.0241740.ref007","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1038\/s41586-020-2008-3","article-title":"A new coronavirus associated with human respiratory disease in China","volume":"579","author":"F Wu","year":"2020","journal-title":"Nature"},{"issue":"3","key":"pone.0241740.ref008","article-title":"Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR.","volume":"25","author":"VM Corman","year":"2020","journal-title":"Euro surveillance: bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin."},{"key":"pone.0241740.ref009","doi-asserted-by":"crossref","first-page":"7633","DOI":"10.1002\/anie.200602772","article-title":"Evolving thermostable reverse transcriptase activity in a DNA polymerase scaffold","volume":"45","author":"KB Sauter","year":"2006","journal-title":"Angew Chem Int Ed Engl"},{"key":"pone.0241740.ref010","doi-asserted-by":"crossref","first-page":"11935","DOI":"10.1002\/anie.201306655","article-title":"Structure and function of an RNA-reading thermostable DNA polymerase","volume":"52","author":"N Blatter","year":"2013","journal-title":"Angew Chem Int Ed Engl"},{"key":"pone.0241740.ref011","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/0092-8674(89)90124-4","article-title":"Characterization of an efficient coronavirus ribosomal frameshifting signal: requirement for an RNA pseudoknot","volume":"57","author":"I Brierley","year":"1989","journal-title":"Cell"},{"key":"pone.0241740.ref012","doi-asserted-by":"crossref","first-page":"e172","DOI":"10.1371\/journal.pbio.0030172","article-title":"A three-stemmed mRNA pseudoknot in the SARS coronavirus frameshift signal","volume":"3","author":"EP Plant","year":"2005","journal-title":"PLoS Biol"},{"key":"pone.0241740.ref013","doi-asserted-by":"crossref","first-page":"e1005473","DOI":"10.1371\/journal.ppat.1005473","article-title":"High-Resolution Analysis of Coronavirus Gene Expression by RNA Sequencing and Ribosome Profiling.","volume":"12","author":"N Irigoyen","year":"2016","journal-title":"PLoS Path"},{"key":"pone.0241740.ref014","doi-asserted-by":"crossref","first-page":"1698","DOI":"10.7150\/ijbs.45357","article-title":"Evaluation of SARS-CoV-2 RNA shedding in clinical specimens and clinical characteristics of 10 patients with COVID-19 in Macau.","volume":"16","author":"IL Lo","year":"2020","journal-title":"Int J Biol Sci."},{"key":"pone.0241740.ref015","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/S1473-3099(20)30113-4","article-title":"Viral load of SARS-CoV-2 in clinical samples","volume":"20","author":"Y Pan","year":"2020","journal-title":"The Lancet Infectious Diseases"},{"key":"pone.0241740.ref016","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1056\/NEJMc2001737","article-title":"SARS-CoV-2 Viral Load in Upper Respiratory Specimens of Infected Patients","volume":"382","author":"L Zou","year":"2020","journal-title":"The New England Journal of Medicine"},{"key":"pone.0241740.ref017","article-title":"Quantitative Detection and Viral Load Analysis of SARS-CoV-2 in Infected Patients","author":"F Yu","year":"2020","journal-title":"Clinical infectious Diseases"},{"key":"pone.0241740.ref018","article-title":"Clinical and virological data of the first cases of COVID-19 in Europe: a case series","author":"FX Lescure","year":"2020","journal-title":"The Lancet Infectious Diseases"},{"key":"pone.0241740.ref019","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1038\/s41591-020-0869-5","article-title":"Temporal dynamics in viral shedding and transmissibility of COVID-19","volume":"26","author":"X He","year":"2020","journal-title":"Nature Medicine"},{"key":"pone.0241740.ref020","article-title":"Predicting infectious SARS-CoV-2 from diagnostic samples","author":"J Bullard","year":"2020","journal-title":"Clinical Infectious Diseases"},{"key":"pone.0241740.ref021","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1038\/s41586-020-2196-x","article-title":"Virological assessment of hospitalized patients with COVID-2019.","volume":"581","author":"R Wolfel","year":"2020","journal-title":"Nature"},{"key":"pone.0241740.ref022","doi-asserted-by":"crossref","first-page":"3838","DOI":"10.1128\/JCM.40.10.3838-3840.2002","article-title":"Impact of nasopharyngeal swab types on detection of Bordetella pertussis by PCR and culture","volume":"40","author":"JL Cloud","year":"2002","journal-title":"Journal of Clinical Microbiology"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0241740","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,2]],"date-time":"2020-11-02T13:39:16Z","timestamp":1604324356000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0241740"}},"subtitle":[],"editor":[{"given":"Massimo","family":"Caputi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,11,2]]},"references-count":22,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2020,11,2]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0241740","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.05.19.20103150","asserted-by":"object"}]},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,2]]}}}