{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T15:57:46Z","timestamp":1765382266111,"version":"3.37.3"},"reference-count":50,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2020,11,5]],"date-time":"2020-11-05T00:00:00Z","timestamp":1604534400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["UMO-2015\/18\/E\/NZ6\/00502"],"award-info":[{"award-number":["UMO-2015\/18\/E\/NZ6\/00502"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The present study compares the immunogenic patterns of muscle larvae excretory-secretory proteins (ML E-S) from <jats:italic>T<\/jats:italic>. <jats:italic>spiralis<\/jats:italic> and <jats:italic>T<\/jats:italic>. <jats:italic>britovi<\/jats:italic> recognized by <jats:italic>Trichinella<\/jats:italic>-infected human sera. Samples were analyzed using two-dimensional electrophoresis (2-DE) coupled with 2D-immunoblot and liquid chromatography-tandem mass spectrometry LC-MS\/MS analysis, two ELISA procedures and a confirmatory 1D-immunoblot test. Sera were obtained from nine patients with a history of ingestion of raw or undercooked meat who presented typical clinical manifestations of trichinellosis and from eleven healthy people. Specific anti-<jats:italic>Trichinella<\/jats:italic> IgG antibodies were detected in all samples tested with the Home-ELISA kits, but in only four samples for the commercially-available kit. The 1D-immunoblot results indicated that all nine serum samples were positive for <jats:italic>T<\/jats:italic>. <jats:italic>spiralis<\/jats:italic> ML E-S antigens, expressed as the presence of specific bands. In contrast, eight of the serum samples with <jats:italic>T<\/jats:italic>. <jats:italic>britovi<\/jats:italic> E-S ML antigens were positive, with one serum sample taken from a patient at 33dpi (days post infection) being negative. To identify immunoreactive proteins that are specifically recognized by host antibodies, both species of ML E-S proteins were subjected to 2D-immunoblotting with human serum taken at 49 dpi. The sera recognized 22 protein spots for <jats:italic>T<\/jats:italic>. <jats:italic>spiralis<\/jats:italic> and 18 for <jats:italic>T<\/jats:italic>. <jats:italic>britovi<\/jats:italic> in 2D-immunoblot analysis. Their molecular weights (MW) ranged from 50 to 60 kDa. LC-MS\/MS analysis identified both common and specifically-recognized immunoreactive proteins: transmembrane serine protease 9, serine protease, antigen targeted by protective antibodies and Actin-1 partial were shared for both <jats:italic>Trichinella<\/jats:italic> species; hypothetical protein T01_7775 and P49 antigen, partial those specific to <jats:italic>T<\/jats:italic>. <jats:italic>spiralis<\/jats:italic>; deoxyribonuclease-2-alpha and hypothetical protein T03_17187\/T12_7360 were specific to <jats:italic>T<\/jats:italic>. <jats:italic>britovi<\/jats:italic>. Our results demonstrate the value of 2-DE and 2D-immunblot as versatile tools for pinpointing factors contributing to the parasite-host relationship by comparing the secretomes of different <jats:italic>Trichinella<\/jats:italic> species.<\/jats:p>","DOI":"10.1371\/journal.pone.0241918","type":"journal-article","created":{"date-parts":[[2020,11,5]],"date-time":"2020-11-05T18:52:22Z","timestamp":1604602342000},"page":"e0241918","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":14,"title":["Immunoproteomic analysis of Trichinella spiralis and Trichinella britovi excretory-secretory muscle larvae proteins recognized by sera from humans infected with 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