{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T13:06:10Z","timestamp":1781701570508,"version":"3.54.5"},"reference-count":18,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2020,11,9]],"date-time":"2020-11-09T00:00:00Z","timestamp":1604880000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    The coronavirus disease 2019 (Covid-19) pandemic, caused by SARS-CoV-2, has resulted in a global testing supply shortage. In response, pooled testing has emerged as a promising strategy that can immediately increase testing capacity. In pooled sample testing, multiple samples are combined (or pooled) together and tested as a single unit. If the pool is positive, the individual samples can then be individually tested to identify the positive case(s). Here, we provide support for the adoption of sample pooling with the point-of-care Cepheid Xpert\n                    <jats:sup>\u00ae<\/jats:sup>\n                    Xpress SARS-CoV-2 molecular assay. Corroborating previous findings, the limit of detection of this assay was comparable to laboratory-developed reverse-transcription quantitative PCR SARS-CoV-2 tests, with observed detection below 100 copies\/mL. The Xpert\n                    <jats:sup>\u00ae<\/jats:sup>\n                    Xpress assay detected SARS-CoV-2 after samples with minimum viral loads of 461 copies\/mL were pooled in groups of six. Based on these data, we recommend the adoption of pooled testing with the Xpert\n                    <jats:sup>\u00ae<\/jats:sup>\n                    Xpress SARS-CoV-2 assay where warranted based on public health needs. The suggested number of samples per pool, or the pooling depth, is unique for each point-of-care testing site and can be determined by the positive test rates. To statistically determine appropriate pooling depth, we have calculated the pooling efficiency for numerous combinations of pool sizes and test rates. This information is included as a supplemental dataset that we encourage public health authorities to use as a guide to make recommendations that will maximize testing capacity and resource conservation.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0241959","type":"journal-article","created":{"date-parts":[[2020,11,9]],"date-time":"2020-11-09T13:34:53Z","timestamp":1604928893000},"page":"e0241959","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":30,"title":["Recommendations for sample pooling on the Cepheid GeneXpert\u00ae system using the Cepheid Xpert\u00ae Xpress SARS-CoV-2 assay"],"prefix":"10.1371","volume":"15","author":[{"given":"Michael G.","family":"Becker","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tracy","family":"Taylor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Kiazyk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dana R.","family":"Cabiles","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrienne F. A.","family":"Meyers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5952-3664","authenticated-orcid":true,"given":"Paul A.","family":"Sandstrom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2020,11,9]]},"reference":[{"key":"pone.0241959.ref001","article-title":"Sample Pooling as a Strategy to Detect Community Transmission of SARS-CoV-2","author":"CA Hogan","year":"2020","journal-title":"JAMA\u2014Journal of the American Medical Association"},{"key":"pone.0241959.ref002","first-page":"30362","article-title":"Pooling of samples for testing for SARS-CoV-2 in asymptomatic people","author":"S Lohse","year":"2020","journal-title":"The Lancet Infectious DiseasesLancet"},{"key":"pone.0241959.ref003","article-title":"Efficient high throughput SARS-CoV-2 testing to detect asymptomatic carriers","author":"N Shental","year":"2020","journal-title":"medRxiv"},{"key":"pone.0241959.ref004","doi-asserted-by":"crossref","first-page":"2000045","DOI":"10.2807\/1560-7917.ES.2020.25.3.2000045","article-title":"Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR","volume":"25","author":"VM Corman","year":"2020","journal-title":"Eurosurveillance"},{"key":"pone.0241959.ref005","article-title":"Comparison of Cepheid Xpert Xpress and Abbott ID Now to Roche cobas for the Rapid Detection of SARS-CoV-2","author":"MC Smithgall","year":"2020","journal-title":"bioRxiv"},{"key":"pone.0241959.ref006","article-title":"Clinical Evaluation of Three Sample-To-Answer Platforms for the Detection of SARS-CoV-2","author":"W Zhen","year":"2020","journal-title":"J Clin Microbiol"},{"key":"pone.0241959.ref007","article-title":"The Detection of SARS-CoV-2 using the Cepheid Xpert Xpress SARS-CoV-2 and Roche cobas SARS-CoV-2 Assays","author":"A Moran","year":"2020","journal-title":"J Clin Microbiol"},{"key":"pone.0241959.ref008","article-title":"Comparison of Commercially Available and Laboratory Developed Assays for in vitro Detection of SARS-CoV-2 in Clinical Laboratories","author":"J Lieberman","year":"2020","journal-title":"medRxiv."},{"key":"pone.0241959.ref009","article-title":"Boosting test-efficiency by pooled testing strategies for SARS-CoV-2","author":"R Hanel","year":"2020"},{"key":"pone.0241959.ref010","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/S1473-3099(20)30113-4","article-title":"Viral load of SARS-CoV-2 in clinical samples","author":"Y Pan","year":"2020","journal-title":"The Lancet Infectious Diseases. Lancet Publishing Group"},{"key":"pone.0241959.ref011","article-title":"Viral dynamics of SARS-CoV-2 across a spectrum of disease severity in COVID-19","author":"G Lui","year":"2020","journal-title":"J Infect"},{"key":"pone.0241959.ref012","first-page":"377","article-title":"Asymptomatic and presymptomatic SARS-COV-2 infections in residents of a long-term care skilled nursing facility\u2014King County, Washington, March 2020","author":"A Kimball","journal-title":"Morbidity and Mortality Weekly Report"},{"key":"pone.0241959.ref013","article-title":"SARS-CoV-2 asymptomatic and symptomatic patients and risk for transfusion transmission","author":"VM Corman","year":"2020","journal-title":"medRxiv"},{"key":"pone.0241959.ref014","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1056\/NEJMc2001737","article-title":"SARS-CoV-2 Viral Load in Upper Respiratory Specimens of Infected Patients","volume":"382","author":"L Zou","year":"2020","journal-title":"N Engl J Med"},{"key":"pone.0241959.ref015","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1007\/s10096-020-03913-9","article-title":"Viral RNA load as determined by cell culture as a management tool for discharge of SARS-CoV-2 patients from infectious disease wards","volume":"39","author":"B La Scola","year":"2020","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"pone.0241959.ref016","first-page":"1","article-title":"Duration of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infectivity: When Is It Safe to Discontinue Isolation?","author":"C Rhee","year":"2020","journal-title":"Clin Infect Dis"},{"key":"pone.0241959.ref017","article-title":"Evaluation of Group Testing for SARS-CoV-2","author":"N Sinnott-Armstrong","year":"2020","journal-title":"RNA. medRxiv"},{"key":"pone.0241959.ref018","unstructured":"Goethe-Universit\u00e4t. Pool testing of SARS-CoV-02 samples increases worldwide test capacities many times over. 2020 [cited 30 Mar 2020]. Available: https:\/\/aktuelles.uni-frankfurt.de\/englisch\/pool-testing-of-sars-cov-02-samples-increases-worldwide-test-capacities-many-times-over\/"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0241959","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,9]],"date-time":"2020-11-09T13:35:25Z","timestamp":1604928925000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0241959"}},"subtitle":[],"editor":[{"given":"Jean-Luc EPH","family":"Darlix","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,11,9]]},"references-count":18,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2020,11,9]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0241959","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.05.14.097287","asserted-by":"object"}]},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,9]]}}}