{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T04:40:05Z","timestamp":1789706405802,"version":"4.0.1"},"reference-count":46,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2020,11,5]],"date-time":"2020-11-05T00:00:00Z","timestamp":1604534400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y Tecnicas","award":["PIP 0426"],"award-info":[{"award-number":["PIP 0426"]}]},{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PICT2017-0183"],"award-info":[{"award-number":["PICT2017-0183"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Fundacion Cientifica Felipe Fiorellino - Universidad Maimonides","award":["Intramural fundings"],"award-info":[{"award-number":["Intramural fundings"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p><jats:italic>Escherichia coli<\/jats:italic> is the most frequent agent of urinary tract infections in humans. The emergence of uropathogenic multidrug-resistant (MDR) <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> strains that produce extended spectrum \u03b2-lactamases (ESBL) has created additional problems in providing adequate treatment of urinary tract infections. We have previously reported the antimicrobial activity of 1,8-cineole, one of the main components of <jats:italic>Rosmarinus officinalis<\/jats:italic> volatile oil, against Gram negative bacteria during planktonic growth. Here, we evaluated the antibiofilm activity of 1,8-cineole against pre-formed mature biofilms of MDR ESBL-producing uropathogenic <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> clinical strains by carrying out different technical approaches such as counting of viable cells, determination of biofilm biomass by crystal violet staining, and live\/dead stain for confocal microscopy and flow cytometric analyses. The plant compound showed a concentration- and time-dependent antibiofilm activity over pre-formed biofilms. After a 1 h treatment with 1% (v\/v) 1,8-cineole, a significant decrease in viable biofilm cell numbers (3-log reduction) was observed. Biofilms of antibiotic-sensitive and MDR ESBL-producing <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> isolates were sensitive to 1,8-cineole exposure. The phytochemical treatment diminished the biofilm biomass by 48\u201365% for all four <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> strain tested. Noteworthy, a significant cell death in the remaining biofilm was confirmed by confocal laser scanning microscopy after live\/dead staining. In addition, the majority of the biofilm-detached cells after 1,8-cineole treatment were dead, as shown by flow cytometric assessment of live\/dead-stained bacteria. Moreover, phytochemical-treated biofilms did not fully recover growth after 24 h in fresh medium. Altogether, our results support the efficacy of 1,8-cineole as a potential antimicrobial agent for the treatment of <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> biofilm-associated infections.<\/jats:p>","DOI":"10.1371\/journal.pone.0241978","type":"journal-article","created":{"date-parts":[[2020,11,5]],"date-time":"2020-11-05T18:52:12Z","timestamp":1604602332000},"page":"e0241978","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":29,"title":["Cell death and biomass reduction in biofilms of multidrug resistant extended spectrum \u03b2-lactamase-producing uropathogenic Escherichia coli isolates by 1,8-cineole"],"prefix":"10.1371","volume":"15","author":[{"given":"Nicolas M.","family":"Vazquez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florencia","family":"Mariani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo S.","family":"Torres","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Moreno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8940-0873","authenticated-orcid":true,"given":"Estela M.","family":"Galv\u00e1n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2020,11,5]]},"reference":[{"key":"pone.0241978.ref001","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1038\/nrmicro3432","article-title":"Urinary tract infections: epidemiology, mechanisms of infection and treatment 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