{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T14:05:16Z","timestamp":1740146716412,"version":"3.37.3"},"reference-count":53,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2020,12,14]],"date-time":"2020-12-14T00:00:00Z","timestamp":1607904000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["5P20GM109094-05"],"award-info":[{"award-number":["5P20GM109094-05"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p><jats:italic>Cryptococcus neoformans<\/jats:italic>is a pathogenic basidiomycetous yeast that primarily infects immunocompromised individuals.<jats:italic>C<\/jats:italic>.<jats:italic>neoformans<\/jats:italic>can thrive during infections due to its three main virulence-related characteristics: the ability to grow at host temperature (37\u00b0C), formation of carbohydrate capsule, and its ability to produce melanin.<jats:italic>C<\/jats:italic>.<jats:italic>neoformans<\/jats:italic>strains lacking septin proteins Cdc3 or Cdc12 are viable at 25\u00b0C; however, they fail to proliferate at 37\u00b0C and are avirulent in the murine model of infection. The basis of septin contribution to growth at host temperature remains unknown. Septins are a family of conserved filament-forming GTPases with roles in cytokinesis and morphogenesis. In the model organism<jats:italic>Saccharomyces cerevisiae<\/jats:italic>septins are essential.<jats:italic>S<\/jats:italic>.<jats:italic>cerevisiae<\/jats:italic>septins form a higher order complex at the mother-bud neck to scaffold over 80 proteins, including those involved in cell wall organization, cell polarity, and cell cycle control. In<jats:italic>C<\/jats:italic>.<jats:italic>neoformans<\/jats:italic>, septins also form a complex at the mother-bud neck but the septin interacting proteome in this species remains largely unknown. Moreover, it remains possible that septins play other roles important for high temperature stress that are independent of their established role in cytokinesis. Therefore, we propose to perform a global analysis of septin Cdc10 binding partners in<jats:italic>C<\/jats:italic>.<jats:italic>neoformans<\/jats:italic>, including those that are specific to high temperature stress. This analysis will shed light on the underlying mechanism of survival of this pathogenic yeast during infection and can potentially lead to the discovery of novel drug targets.<\/jats:p>","DOI":"10.1371\/journal.pone.0242381","type":"journal-article","created":{"date-parts":[[2020,12,14]],"date-time":"2020-12-14T18:30:08Z","timestamp":1607970608000},"page":"e0242381","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Registered report protocol: Quantitative analysis of septin Cdc10-associated proteome in Cryptococcus neoformans"],"prefix":"10.1371","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9350-3576","authenticated-orcid":true,"given":"Stephani","family":"Martinez Barrera","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie","family":"Byrum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel G.","family":"Mackintosh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9560-782X","authenticated-orcid":true,"given":"Lukasz","family":"Kozubowski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2020,12,14]]},"reference":[{"issue":"8","key":"pone.0242381.ref001","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1016\/S1473-3099(17)30243-8","article-title":"Global burden of disease of HIV-associated cryptococcal meningitis: an updated analysis","volume":"17","author":"R Rajasingham","year":"2017","journal-title":"Lancet Infect Dis"},{"issue":"3","key":"pone.0242381.ref002","doi-asserted-by":"crossref","first-page":"375","DOI":"10.3201\/eid0703.017302","article-title":"Cryptococcus neoformans infection in organ transplant recipients: variables influencing clinical characteristics and outcome","volume":"7","author":"S Husain","year":"2001","journal-title":"Emerg Infect Dis"},{"issue":"6","key":"pone.0242381.ref003","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1128\/EC.00039-10","article-title":"Cryptococcal Interactions with the Host Immune System","volume":"9","author":"K Voelz","year":"2010","journal-title":"Eukaryot Cell"},{"issue":"4","key":"pone.0242381.ref004","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1128\/CMR.8.4.515","article-title":"Cryptococcosis in the era of AIDS\u2014100 years after the discovery of Cryptococcus neoformans.","volume":"8","author":"TG Mitchell","year":"1995","journal-title":"Clin Microbiol Rev"},{"issue":"1","key":"pone.0242381.ref005","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1111\/j.1574-6976.2011.00286.x","article-title":"Profiling a killer, the development of Cryptococcus neoformans","volume":"36","author":"L Kozubowski","year":"2012","journal-title":"FEMS Microbiol Rev"},{"issue":"10","key":"pone.0242381.ref006","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1038\/nrmicro1245","article-title":"Deciphering the model pathogenic fungus Cryptococcus neoformans","volume":"3","author":"A Idnurm","year":"2005","journal-title":"Nat Rev Microbiol"},{"issue":"January","key":"pone.0242381.ref007","first-page":"1","article-title":"Cryptococcus neoformans Intracellular Proliferation and Capsule Size Determines Early Macrophage Control of Infection. 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