{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T21:15:17Z","timestamp":1784063717346,"version":"3.55.0"},"reference-count":65,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2020,12,7]],"date-time":"2020-12-07T00:00:00Z","timestamp":1607299200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["EY025601"],"award-info":[{"award-number":["EY025601"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Clayton Foundation for Research"},{"DOI":"10.13039\/100001818","name":"Research to Prevent Blindness","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001818","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    Cell culture is widely utilized to study the cellular and molecular biology of different neuronal cell populations. Current techniques to study enriched neurons\n                    <jats:italic>in vitro<\/jats:italic>\n                    are primarily limited to embryonic\/neonatal animals and induced pluripotent stem cells (iPSCs). Although the use of these cultures is valuable, the accessibility of purified primary adult neuronal cultures would allow for improved assessment of certain neurological diseases and pathways at the cellular level. Using a modified 7-step immunopanning technique to isolate for retinal ganglion cells (RGCs) and amacrine cells (ACs) from adult mouse retinas, we have successfully developed a model of neuronal culture that maintains for at least one week. Isolations of Thy1.2\n                    <jats:sup>+<\/jats:sup>\n                    cells are enriched for RGCs, with the isolation cell yield being congruent to the theoretical yield of RGCs in a mouse retina. ACs of two different populations (CD15\n                    <jats:sup>+<\/jats:sup>\n                    and CD57\n                    <jats:sup>+<\/jats:sup>\n                    ) can also be isolated. The populations of these three adult neurons in culture are healthy, with neurite outgrowths in some cases greater than 500\u03bcm in length. Optimization of culture conditions for RGCs and CD15\n                    <jats:sup>+<\/jats:sup>\n                    cells revealed that neuronal survival and the likelihood of neurite outgrowth respond inversely to different culture media. Serially diluted concentrations of puromycin decreased cultured adult RGCs in a dose-dependent manner, demonstrating the potential usefulness of these adult neuronal cultures in screening assays. This novel culture system can be used to model\n                    <jats:italic>in vivo<\/jats:italic>\n                    neuronal behaviors. Studies can now be expanded in conjunction with other methodologies to study the neurobiology of function, aging, and diseases.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0242426","type":"journal-article","created":{"date-parts":[[2020,12,7]],"date-time":"2020-12-07T16:02:07Z","timestamp":1607356927000},"page":"e0242426","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":12,"title":["Optimized culture of retinal ganglion cells and amacrine cells from adult mice"],"prefix":"10.1371","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7343-5652","authenticated-orcid":true,"given":"Yong H.","family":"Park","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua D.","family":"Snook","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iris","family":"Zhuang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guofu","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8096-9812","authenticated-orcid":true,"given":"Benjamin J.","family":"Frankfort","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2020,12,7]]},"reference":[{"issue":"2","key":"pone.0242426.ref001","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.jneumeth.2005.05.022","article-title":"Optimized protocols for the simultaneous preparation of primary neuronal cultures of the neocortex, hippocampus and cerebellum from individual newborn (P0.5) C57Bl\/6J mice","volume":"149","author":"B Ahlemeyer","year":"2005","journal-title":"J Neurosci Methods"},{"issue":"9","key":"pone.0242426.ref002","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1016\/0896-6273(88)90127-4","article-title":"Immunological, morphological, and electrophysiological variation among retinal ganglion cells purified by panning","volume":"1","author":"BA Barres","year":"1988","journal-title":"Neuron"},{"issue":"4","key":"pone.0242426.ref003","first-page":"319","article-title":"Purification and culture of spinal motor neurons from rat embryos","volume":"2013","author":"DJ Graber","year":"2013","journal-title":"Cold Spring Harb Protoc"},{"issue":"9","key":"pone.0242426.ref004","doi-asserted-by":"crossref","first-page":"1741","DOI":"10.1038\/nprot.2012.099","article-title":"Culturing pyramidal neurons from the early postnatal mouse hippocampus and cortex","volume":"7","author":"GM Beaudoin","year":"2012","journal-title":"Nat Protoc"},{"key":"pone.0242426.ref005","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.jneumeth.2016.02.008","article-title":"Long-term primary culture of neurons taken from chick embryo brain: A model to study neural cell biology, synaptogenesis and its dynamic properties","volume":"263","author":"A Kumar","year":"2016","journal-title":"J Neurosci Methods"},{"issue":"2","key":"pone.0242426.ref006","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1167\/iovs.17-22406","article-title":"Culture Systems of Dissociated Mouse and Human Pluripotent Stem Cell-Derived Retinal Ganglion Cells Purified by Two-Step Immunopanning","volume":"59","author":"W Kobayashi","year":"2018","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"1","key":"pone.0242426.ref007","doi-asserted-by":"crossref","first-page":"e98","DOI":"10.1002\/cpcb.98","article-title":"Preparation and Co-Culture of iPSC-Derived Dopaminergic Neurons and Astrocytes","volume":"85","author":"A de Rus Jacquet","year":"2019","journal-title":"Curr Protoc Cell Biol"},{"key":"pone.0242426.ref008","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.neuro.2018.06.007","article-title":"Human iPSC-derived neuronal models for in vitro neurotoxicity assessment","volume":"67","author":"AM Tukker","year":"2018","journal-title":"Neurotoxicology"},{"issue":"6","key":"pone.0242426.ref009","doi-asserted-by":"crossref","first-page":"1490","DOI":"10.1038\/nprot.2007.207","article-title":"Isolation and culture of adult neurons and neurospheres","volume":"2","author":"GJ Brewer","year":"2007","journal-title":"Nat Protoc"},{"issue":"3","key":"pone.0242426.ref010","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/0006-8993(77)90594-7","article-title":"Rat hippocampal neurons in dispersed cell culture","volume":"126","author":"GA Banker","year":"1977","journal-title":"Brain Res"},{"issue":"10","key":"pone.0242426.ref011","doi-asserted-by":"crossref","first-page":"1214","DOI":"10.1091\/mbc.E18-06-0361","article-title":"Nestin in immature embryonic neurons affects axon growth cone morphology and Semaphorin3a sensitivity","volume":"30","author":"CJ Bott","year":"2019","journal-title":"Mol Biol Cell"},{"issue":"7","key":"pone.0242426.ref012","doi-asserted-by":"crossref","first-page":"3800","DOI":"10.1167\/iovs.09-4540","article-title":"Amacrine cell gene expression and survival signaling: differences from neighboring retinal ganglion cells","volume":"51","author":"NJ Kunzevitzky","year":"2010","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"12","key":"pone.0242426.ref013","doi-asserted-by":"crossref","first-page":"3525","DOI":"10.1021\/acsbiomaterials.7b00596","article-title":"Comparison of NMDA and AMPA Channel Expression and Function between Embryonic and Adult Neurons Utilizing Microelectrode Array Systems","volume":"3","author":"D Edwards","year":"2017","journal-title":"ACS Biomater Sci Eng"},{"issue":"2","key":"pone.0242426.ref014","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.molmed.2013.11.004","article-title":"iPSC-derived neurons as a higher-throughput readout for autism: promises and pitfalls","volume":"20","author":"D Prilutsky","year":"2014","journal-title":"Trends Mol Med"},{"issue":"5","key":"pone.0242426.ref015","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1093\/hmg\/ddv637","article-title":"Assessing similarity to primary tissue and cortical layer identity in induced pluripotent stem cell-derived cortical neurons through single-cell transcriptomics","volume":"25","author":"AE Handel","year":"2016","journal-title":"Hum Mol Genet"},{"issue":"1","key":"pone.0242426.ref016","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1038\/nrneurol.2012.227","article-title":"The retina as a window to the brain-from eye research to CNS disorders","volume":"9","author":"A London","year":"2013","journal-title":"Nat Rev Neurol"},{"issue":"6","key":"pone.0242426.ref017","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1002\/cne.21368","article-title":"Molecular heterogeneity of developing retinal ganglion and amacrine cells revealed through single cell gene expression profiling","volume":"502","author":"JM Trimarchi","year":"2007","journal-title":"J Comp Neurol"},{"issue":"4","key":"pone.0242426.ref018","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1369\/0022155416689675","article-title":"Immunocytochemical Profiling of Cultured Mouse Primary Retinal Cells","volume":"65","author":"MC Zalis","year":"2017","journal-title":"J Histochem Cytochem"},{"issue":"6","key":"pone.0242426.ref019","doi-asserted-by":"crossref","first-page":"1411","DOI":"10.1002\/cne.23521","article-title":"The RNA binding protein RBPMS is a selective marker of ganglion cells in the mammalian retina","volume":"522","author":"AR Rodriguez","year":"2014","journal-title":"J Comp Neurol"},{"issue":"5","key":"pone.0242426.ref020","doi-asserted-by":"crossref","first-page":"1202","DOI":"10.1016\/j.cell.2015.05.002","article-title":"Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets","volume":"161","author":"EZ Macosko","year":"2015","journal-title":"Cell"},{"issue":"1","key":"pone.0242426.ref021","doi-asserted-by":"crossref","first-page":"2759","DOI":"10.1038\/s41467-018-05134-3","article-title":"Single cell transcriptome profiling of retinal ganglion cells identifies cellular subtypes","volume":"9","author":"BA Rheaume","year":"2018","journal-title":"Nat Commun"},{"issue":"5","key":"pone.0242426.ref022","doi-asserted-by":"crossref","first-page":"1308","DOI":"10.1016\/j.cell.2016.07.054","article-title":"Comprehensive Classification of Retinal Bipolar Neurons by Single-Cell Transcriptomics","volume":"166","author":"K Shekhar","year":"2016","journal-title":"Cell"},{"issue":"3","key":"pone.0242426.ref023","doi-asserted-by":"crossref","first-page":"1945","DOI":"10.1152\/jn.00142.2009","article-title":"Genetic dissection of rod and cone pathways in the dark-adapted mouse retina","volume":"102","author":"MM Abd-El-Barr","year":"2009","journal-title":"J Neurophysiol"},{"issue":"1","key":"pone.0242426.ref024","doi-asserted-by":"crossref","first-page":"4902","DOI":"10.1038\/s41467-019-12780-8","article-title":"Single-cell transcriptomic atlas of the human retina identifies cell types associated with age-related macular degeneration","volume":"10","author":"M Menon","year":"2019","journal-title":"Nat Commun"},{"issue":"27","key":"pone.0242426.ref025","doi-asserted-by":"crossref","first-page":"5177","DOI":"10.1523\/JNEUROSCI.0471-20.2020","article-title":"Mouse Retinal Cell Atlas: Molecular Identification of over Sixty Amacrine Cell Types","volume":"40","author":"W Yan","year":"2020","journal-title":"J Neurosci"},{"issue":"5","key":"pone.0242426.ref026","doi-asserted-by":"crossref","first-page":"1644","DOI":"10.1016\/j.celrep.2020.01.007","article-title":"Single-Cell Transcriptomic Comparison of Human Fetal Retina, hPSC-Derived Retinal Organoids, and Long-Term Retinal Cultures","volume":"30","author":"A Sridhar","year":"2020","journal-title":"Cell Rep"},{"issue":"18","key":"pone.0242426.ref027","doi-asserted-by":"crossref","first-page":"e100811","DOI":"10.15252\/embj.2018100811","article-title":"A single-cell transcriptome atlas of the adult human retina","volume":"38","author":"SW Lukowski","year":"2019","journal-title":"EMBO J"},{"key":"pone.0242426.ref028","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1146\/annurev-neuro-071714-034120","article-title":"The types of retinal ganglion cells: current status and implications for neuronal classification","volume":"38","author":"JR Sanes","year":"2015","journal-title":"Annu Rev Neurosci"},{"issue":"21","key":"pone.0242426.ref029","doi-asserted-by":"crossref","first-page":"8936","DOI":"10.1523\/JNEUROSCI.18-21-08936.1998","article-title":"The major cell populations of the mouse retina","volume":"18","author":"CJ Jeon","year":"1998","journal-title":"J Neurosci"},{"issue":"2","key":"pone.0242426.ref030","doi-asserted-by":"crossref","first-page":"e0117615","DOI":"10.1371\/journal.pone.0117615","article-title":"TRPM3 expression in mouse retina","volume":"10","author":"RL Brown","year":"2015","journal-title":"PLoS One"},{"issue":"14","key":"pone.0242426.ref031","doi-asserted-by":"crossref","first-page":"5495","DOI":"10.1523\/JNEUROSCI.0187-11.2011","article-title":"Otx2 promotes the survival of damaged adult retinal ganglion cells and protects against excitotoxic loss of visual acuity in vivo","volume":"31","author":"R Torero Ibad","year":"2011","journal-title":"J Neurosci"},{"issue":"2","key":"pone.0242426.ref032","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1007\/s00441-009-0897-4","article-title":"Trophic responsiveness of purified postnatal and adult rat retinal ganglion cells","volume":"339","author":"CH Ma","year":"2010","journal-title":"Cell Tissue Res"},{"issue":"5","key":"pone.0242426.ref033","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1167\/iovs.03-0406","article-title":"Acetylcholine protection of adult pig retinal ganglion cells from glutamate-induced excitotoxicity","volume":"45","author":"E Wehrwein","year":"2004","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"pone.0242426.ref034","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.neuroscience.2013.02.003","article-title":"Neuroprotection of rat retinal ganglion cells mediated through alpha7 nicotinic acetylcholine receptors","volume":"237","author":"K Iwamoto","year":"2013","journal-title":"Neuroscience"},{"issue":"14","key":"pone.0242426.ref035","doi-asserted-by":"crossref","first-page":"3425","DOI":"10.1113\/jphysiol.2008.154609","article-title":"Glutamatergic calcium dynamics and deregulation of rat retinal ganglion cells","volume":"586","author":"AT Hartwick","year":"2008","journal-title":"J Physiol"},{"issue":"1","key":"pone.0242426.ref036","doi-asserted-by":"crossref","first-page":"2594","DOI":"10.1038\/s41598-019-39141-1","article-title":"Transcriptomic profiles of retinal ganglion cells are defined by the magnitude of intraocular pressure elevation in adult mice","volume":"9","author":"YH Park","year":"2019","journal-title":"Sci Rep"},{"issue":"7","key":"pone.0242426.ref037","first-page":"643","article-title":"Purification and culture of retinal ganglion cells from rodents","volume":"2013","author":"A Winzeler","year":"2013","journal-title":"Cold Spring Harb Protoc"},{"key":"pone.0242426.ref038","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.jneumeth.2016.05.001","article-title":"Establishment of a long-term spiral ganglion neuron culture with reduced glial cell number: Effects of AraC on cell composition and neurons","volume":"268","author":"J Schwieger","year":"2016","journal-title":"J Neurosci Methods"},{"issue":"8","key":"pone.0242426.ref039","doi-asserted-by":"crossref","first-page":"3671","DOI":"10.1021\/acschemneuro.9b00248","article-title":"Cholesterol Biosynthesis and Uptake in Developing Neurons","volume":"10","author":"TC Genaro-Mattos","year":"2019","journal-title":"ACS Chem Neurosci"},{"issue":"5","key":"pone.0242426.ref040","doi-asserted-by":"crossref","first-page":"934","DOI":"10.1002\/jimd.12088","article-title":"Desmosterolosis and desmosterol homeostasis in the developing mouse brain","volume":"42","author":"LB Allen","year":"2019","journal-title":"J Inherit Metab Dis"},{"issue":"5","key":"pone.0242426.ref041","first-page":"546","article-title":"Specific binding of peanut lectin to a class of retinal photoreceptor cells. A species comparison","volume":"25","author":"JC Blanks","year":"1984","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"11","key":"pone.0242426.ref042","doi-asserted-by":"crossref","first-page":"5411","DOI":"10.1167\/iovs.08-3246","article-title":"CD73, a novel cell surface antigen that characterizes retinal photoreceptor precursor cells","volume":"50","author":"H Koso","year":"2009","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"3","key":"pone.0242426.ref043","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1002\/cne.10845","article-title":"CD15 immunoreactive amacrine cells in the mouse retina","volume":"465","author":"TC Jakobs","year":"2003","journal-title":"J Comp Neurol"},{"issue":"10","key":"pone.0242426.ref044","doi-asserted-by":"crossref","first-page":"3044","DOI":"10.1523\/JNEUROSCI.06-10-03044.1986","article-title":"Primary culture of identified neurons from the visual cortex of postnatal rats","volume":"6","author":"JE Huettner","year":"1986","journal-title":"J Neurosci"},{"issue":"12","key":"pone.0242426.ref045","doi-asserted-by":"crossref","first-page":"1342","DOI":"10.1101\/pdb.ip073999","article-title":"Designing and troubleshooting immunopanning protocols for purifying neural cells","volume":"2014","author":"BA Barres","year":"2014","journal-title":"Cold Spring Harb Protoc"},{"key":"pone.0242426.ref046","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1186\/s13287-015-0213-z","article-title":"A puromycin selectable cell line for the enrichment of mouse embryonic stem cell-derived V3 interneurons","volume":"6","author":"H Xu","year":"2015","journal-title":"Stem Cell Res Ther"},{"issue":"2","key":"pone.0242426.ref047","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.neuron.2019.07.014","article-title":"CRISPR Interference-Based Platform for Multimodal Genetic Screens in Human iPSC-Derived Neurons","volume":"104","author":"R Tian","year":"2019","journal-title":"Neuron"},{"issue":"40","key":"pone.0242426.ref048","doi-asserted-by":"crossref","first-page":"29314","DOI":"10.1074\/jbc.M701413200","article-title":"Disruption of telomere maintenance by depletion of the MRE11\/RAD50\/NBS1 complex in cells that use alternative lengthening of telomeres","volume":"282","author":"ZH Zhong","year":"2007","journal-title":"J Biol Chem"},{"issue":"2","key":"pone.0242426.ref049","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1002\/cne.10323","article-title":"Large-scale morphological survey of mouse retinal ganglion cells","volume":"451","author":"W Sun","year":"2002","journal-title":"J Comp Neurol"},{"issue":"7586","key":"pone.0242426.ref050","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1038\/nature16468","article-title":"The functional diversity of retinal ganglion cells in the mouse","volume":"529","author":"T Baden","year":"2016","journal-title":"Nature"},{"issue":"23","key":"pone.0242426.ref051","doi-asserted-by":"crossref","first-page":"7845","DOI":"10.1523\/JNEUROSCI.2960-13.2014","article-title":"Genetically targeted binary labeling of retinal neurons","volume":"34","author":"Y Zhu","year":"2014","journal-title":"J Neurosci"},{"issue":"9","key":"pone.0242426.ref052","doi-asserted-by":"crossref","first-page":"e0137702","DOI":"10.1371\/journal.pone.0137702","article-title":"Response Properties of a Newly Identified Tristratified Narrow Field Amacrine Cell in the Mouse Retina","volume":"10","author":"GS Newkirk","year":"2015","journal-title":"PLoS One"},{"issue":"5","key":"pone.0242426.ref053","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1080\/15384101.2015.1004937","article-title":"Neuronal cell cycle: the neuron itself and its circumstances","volume":"14","author":"JM Frade","year":"2015","journal-title":"Cell Cycle"},{"issue":"1","key":"pone.0242426.ref054","doi-asserted-by":"crossref","first-page":"99","DOI":"10.2144\/05381RR02","article-title":"Culture of adult mouse neurons","volume":"38","author":"L Eide","year":"2005","journal-title":"Biotechniques"},{"issue":"1","key":"pone.0242426.ref055","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jneumeth.2007.12.008","article-title":"Efficient estimation of retinal ganglion cell number: a stereological approach","volume":"170","author":"JB Fileta","year":"2008","journal-title":"J Neurosci Methods"},{"key":"pone.0242426.ref056","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/978-3-030-11135-9_1","article-title":"Past, Present, and Future of Neuronal Models In Vitro","volume":"22","author":"JM Keller","year":"2019","journal-title":"Adv Neurobiol"},{"issue":"5\u20136","key":"pone.0242426.ref057","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1159\/000493018","article-title":"Advances and Current Challenges Associated with the Use of Human Induced Pluripotent Stem Cells in Modeling Neurodegenerative Disease","volume":"205","author":"BJ Berry","year":"2018","journal-title":"Cells Tissues Organs"},{"issue":"6","key":"pone.0242426.ref058","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1016\/j.neuron.2015.02.017","article-title":"Subtype-specific regeneration of retinal ganglion cells following axotomy: effects of osteopontin and mTOR signaling","volume":"85","author":"X Duan","year":"2015","journal-title":"Neuron"},{"issue":"6","key":"pone.0242426.ref059","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1016\/j.neuron.2019.11.006","article-title":"Single-Cell Profiles of Retinal Ganglion Cells Differing in Resilience to Injury Reveal Neuroprotective Genes","volume":"104","author":"NM Tran","year":"2019","journal-title":"Neuron"},{"issue":"12","key":"pone.0242426.ref060","doi-asserted-by":"crossref","first-page":"7603","DOI":"10.1167\/iovs.13-12691","article-title":"Amacrine cell subtypes differ in their intrinsic neurite growth capacity","volume":"54","author":"NJ Kunzevitzky","year":"2013","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"pone.0242426.ref061","doi-asserted-by":"crossref","first-page":"983","DOI":"10.3389\/fnins.2019.00983","article-title":"Adult Goat Retinal Neuronal Culture: Applications in Modeling Hyperglycemia","volume":"13","author":"S Sharma","year":"2019","journal-title":"Front Neurosci"},{"key":"pone.0242426.ref062","doi-asserted-by":"crossref","first-page":"277","DOI":"10.3389\/fncel.2013.00277","article-title":"Strategies for repair of white matter: influence of osmolarity and microglia on proliferation and apoptosis of oligodendrocyte precursor cells in different basal culture media","volume":"7","author":"K Kleinsimlinghaus","year":"2013","journal-title":"Front Cell Neurosci"},{"issue":"2","key":"pone.0242426.ref063","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/S0165-0270(96)00136-7","article-title":"Isolation and culture of adult rat hippocampal neurons","volume":"71","author":"GJ Brewer","year":"1997","journal-title":"J Neurosci Methods"},{"issue":"1","key":"pone.0242426.ref064","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/S0304-3940(01)01676-7","article-title":"cAMP and calcium ionophore induce outgrowth of neuronal processes in PC12 mutant cells in which nerve growth factor-induced outgrowth of neuronal processes is impaired","volume":"303","author":"Y Kano","year":"2001","journal-title":"Neurosci Lett"},{"issue":"1","key":"pone.0242426.ref065","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.ajpath.2017.08.032","article-title":"Cobalamin-Associated Superoxide Scavenging in Neuronal Cells Is a Potential Mechanism for Vitamin B12-Deprivation Optic Neuropathy","volume":"188","author":"W Chan","year":"2018","journal-title":"Am J Pathol"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0242426","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,12,7]],"date-time":"2020-12-07T16:02:55Z","timestamp":1607356975000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0242426"}},"subtitle":[],"editor":[{"given":"Rafael","family":"Linden","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,12,7]]},"references-count":65,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2020,12,7]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0242426","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.06.16.155069","asserted-by":"object"}]},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,12,7]]}}}