{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T22:28:10Z","timestamp":1791930490830,"version":"4.3.4"},"reference-count":23,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2021,3,18]],"date-time":"2021-03-18T00:00:00Z","timestamp":1616025600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100011199","name":"FP7 Ideas: European Research Council","doi-asserted-by":"publisher","award":["2017 FE 9005"],"award-info":[{"award-number":["2017 FE 9005"]}],"id":[{"id":"10.13039\/100011199","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Precise quantification of molecular targets in a biological sample across a wide dynamic range is a key requirement in many diagnostic procedures, such as monitoring response to therapy or detection of measurable residual disease. State of the art digital PCR assays provide for a dynamic range of four orders of magnitude. However digital assays are complex and require sophisticated microfluidic tools. Here we present an assay format that enables ultra-precise quantification of RNA targets in a single measurement across a dynamic range of more than six orders of magnitude. The approach is based on hydrogel beads that provide for microfluidic free compartmentalization of the sample as they are used as nanoreactors for reverse transcription, PCR amplification and combined real time and digital detection of gene transcripts. We have applied these nanoreactor beads for establishing an assay for the detection and quantification of BCR-ABL1 fusion transcripts. The assay has been characterized for its precision and linear dynamic range. A comparison of the new method against conventional real time RT-PCR analysis (reference method) with clinical samples from patients with chronic myeloid leukemia (CML) revealed excellent concordance with Pearsons correlation coefficient of 0.983 and slope of 1.08.<\/jats:p>","DOI":"10.1371\/journal.pone.0242529","type":"journal-article","created":{"date-parts":[[2021,3,18]],"date-time":"2021-03-18T13:50:26Z","timestamp":1616075426000},"page":"e0242529","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Ultra-precise quantification of mRNA targets across a broad dynamic range with nanoreactor beads"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2763-5765","authenticated-orcid":true,"given":"Ivan Francisco","family":"Loncarevic","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Toepfer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"Hubold","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Klingner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lea","family":"Kanitz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Ellinger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrin","family":"Steinmetzer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Ernst","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Hochhaus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugen","family":"Ermantraut","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,3,18]]},"reference":[{"issue":"1","key":"pone.0242529.ref001","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.bdq.2014.06.001","article-title":"Digital PCR: A brief history","volume":"1","author":"AA Morley","year":"2014","journal-title":"Biomolecular detection and quantification"},{"issue":"23","key":"pone.0242529.ref002","doi-asserted-by":"crossref","first-page":"8975","DOI":"10.1021\/ac801276c","article-title":"High-throughput quantitative polymerase chain reaction in picoliter droplets","volume":"80","author":"MM Kiss","year":"2008","journal-title":"Anal Chem"},{"issue":"Supplement C","key":"pone.0242529.ref003","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.copbio.2013.08.013","article-title":"Microfluidics for biological measurements with single-molecule resolution","volume":"25","author":"AM Streets","year":"2014","journal-title":"Current Opinion in Biotechnology"},{"issue":"4","key":"pone.0242529.ref004","article-title":"dPCR: A Technology Review","volume":"18","author":"PL Quan","year":"2018","journal-title":"Sensors (Basel, Switzerland)"},{"key":"pone.0242529.ref005","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.bdq.2016.10.001","article-title":"Digital PCR dynamic range is approaching that of real-time quantitative PCR","volume":"10","author":"GM Jones","year":"2016","journal-title":"Biomolecular detection and quantification"},{"key":"pone.0242529.ref006","unstructured":"Steinmetzer Katrin JDE, Hubold Stephan JDE, Ellinger Thomas JDE, Ermantraut Eugen JDE, Schulz Torsten JDE, inventorsVORGEFERTIGTES MIKROPARTIKEL ZUR DURCHF\u00dcHRUNG EINER DIGITALEN DETEKTION EINES ANALYTEN | MICROPARTICULE PR\u00c9FABRIQU\u00c9E PERMETTANT DE R\u00c9ALISER UNE D\u00c9TECTION NUM\u00c9RIQUE D\u2019UN ANALYTE | A PREFABRICATED MICROPARTICLE FOR PERFORMING A DIGITAL DETECTION OF AN ANALYTE. 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