{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T23:29:59Z","timestamp":1787614199610,"version":"build-2736575974"},"reference-count":101,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2020,12,7]],"date-time":"2020-12-07T00:00:00Z","timestamp":1607299200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000315","name":"Aberystwyth University","doi-asserted-by":"publisher","award":["DCDS PhD studentship"],"award-info":[{"award-number":["DCDS PhD studentship"]}],"id":[{"id":"10.13039\/501100000315","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Preimplantation factor (PIF) is an embryo derived peptide which exerts an immune modulatory effect on human endometrium, promoting immune tolerance to the embryo whilst maintaining the immune response to invading pathogens. While bovine embryos secrete PIF, the effect on the bovine endometrium is unknown. Maternal recognition of pregnancy is driven by an embryo-maternal cross talk, however the process differs between humans and cattle. As many embryos are lost during the early part of pregnancy in cattle, a greater knowledge of factors affecting the embryo-maternal crosstalk, such as PIF, is needed to improve fertility. Therefore, for the first time, we demonstrate the effect of synthetic PIF (sPIF) on the bovine transcriptome in an<jats:italic>ex vivo<\/jats:italic>bovine endometrial tissue culture model. Explants were cultured for 30h with sPIF (100nM) or in control media. Total RNA was analysed via RNA-sequencing. As a result of sPIF treatment, 102 genes were differentially expressed compared to the control (<jats:italic>P<\/jats:italic>adj&lt;0.1), although none by more than 2-fold. The majority of genes (78) were downregulated. Pathway analysis revealed targeting of several immune based pathways. Genes for the TNF, NF-\u03baB, IL-17, MAPK and TLR signalling pathways were down-regulated by sPIF. However, some immune genes were demonstrated to be upregulated following sPIF treatment, including<jats:italic>C3<\/jats:italic>. Steroid biosynthesis was the only over-represented pathway with all genes upregulated. We demonstrate that sPIF can modulate the bovine endometrial transcriptome in an immune modulatory manner, like that in the human endometrium, however, the regulation of genes was much weaker than in previous human work.<\/jats:p>","DOI":"10.1371\/journal.pone.0242874","type":"journal-article","created":{"date-parts":[[2020,12,7]],"date-time":"2020-12-07T22:01:37Z","timestamp":1607378497000},"page":"e0242874","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["Interaction of preimplantation factor with the global bovine endometrial transcriptome"],"prefix":"10.1371","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7732-9483","authenticated-orcid":true,"given":"Ruth E.","family":"Wonfor","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher J.","family":"Creevey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuela","family":"Natoli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Hegarty","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah M.","family":"Nash","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael T.","family":"Rose","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2020,12,7]]},"reference":[{"key":"pone.0242874.ref001","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1186\/1477-7827-9-63","article-title":"PreImplantation Factor (PIF) correlates with early mammalian embryo development-bovine and murine models","volume":"9","author":"CW Stamatkin","year":"2011","journal-title":"Reprod Biol Endocrin"},{"key":"pone.0242874.ref002","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1111\/j.1600-0897.2004.00159.x","article-title":"Insight into early pregnancy events: The emerging role of the embryo","volume":"51","author":"ER Barnea","year":"2004","journal-title":"Am J Reprod Immunol"},{"issue":"1","key":"pone.0242874.ref003","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1186\/1477-7827-11-105","article-title":"PreImplantation factor (PIF) detection in maternal circulation in early pregnancy correlates with live birth (bovine model)","volume":"11","author":"S Ramu","year":"2013","journal-title":"Reprod Biol Endocrin"},{"key":"pone.0242874.ref004","doi-asserted-by":"crossref","unstructured":"Mentorou C, Promponas EM, Keramitsoglou T, Daves S, Mastrominas M, Perros G, et al. Preimplantation factor (PIF*) levels in embryo culture supernatants after single embryo transfer correlate with pregnancy outcome. 8th European Congress on Reproductive Immunology; 2010; Munich, Germany: J Reprod Immunol.","DOI":"10.1016\/j.jri.2010.08.039"},{"issue":"5","key":"pone.0242874.ref005","doi-asserted-by":"crossref","first-page":"459 e1","DOI":"10.1016\/j.ajog.2010.03.024","article-title":"A genomic and proteomic investigation of the impact of preimplantation factor on human decidual cells","volume":"202","author":"MJ Paidas","year":"2010","journal-title":"Am J Obstet Gynecol"},{"issue":"4","key":"pone.0242874.ref006","doi-asserted-by":"crossref","first-page":"313 e1","DOI":"10.1016\/j.ajog.2012.07.017","article-title":"PreImplantation Factor (PIF) orchestrates systemic antiinflammatory response by immune cells: effect on peripheral blood mononuclear cells","volume":"207","author":"ER Barnea","year":"2012a","journal-title":"Am J Obstet Gynecol"},{"key":"pone.0242874.ref007","unstructured":"Immler RT, Barnea ER, Sperandio M. PreImplantation factor (PIF) impairs leukocyte recruitment in TNF-\u03b1 induced inflammation in vivo. 94th Annual Meeting of the German Physiological Society; 2015; Magdeburg, Germany."},{"issue":"7","key":"pone.0242874.ref008","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1016\/j.imbio.2015.01.010","article-title":"PIF direct immune regulation: Blocks mitogen-activated PBMCs proliferation, promotes TH2\/TH1 bias, independent of Ca(2.)","volume":"220","author":"ER Barnea","year":"2015","journal-title":"Immunobiology"},{"issue":"4","key":"pone.0242874.ref009","doi-asserted-by":"crossref","first-page":"402 e1","DOI":"10.1016\/j.ajog.2010.06.060","article-title":"Preimplantation factor promotes first trimester trophoblast invasion","volume":"203","author":"CM Duzyj","year":"2010","journal-title":"Am J Obstet Gynecol"},{"issue":"1","key":"pone.0242874.ref010","doi-asserted-by":"crossref","first-page":"6, 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Mor","year":"2011","journal-title":"Ann N Y Acad Sci"},{"key":"pone.0242874.ref026","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1152\/physiolgenomics.90404.2008","article-title":"Gene expression profiles of bovine caruncular and intercaruncular endometrium at implantation","volume":"39","author":"N Mansouri-Attia","year":"2008","journal-title":"Physiol Genomics"},{"key":"pone.0242874.ref027","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.theriogenology.2017.08.001","article-title":"Effects of preimplantation factor on interleukin-6 and prostaglandin F2\u03b1 and E2 in the bovine endometrium","volume":"102","author":"RE Wonfor","year":"2017","journal-title":"Theriogenology"},{"key":"pone.0242874.ref028","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.anireprosci.2004.04.026","article-title":"Steroidal regulation of uterine immune defenses","volume":"82\u201383","author":"GS Lewis","year":"2004","journal-title":"Anim Reprod 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Arosh","year":"2002","journal-title":"Biol Reprod"},{"issue":"7","key":"pone.0242874.ref032","doi-asserted-by":"crossref","first-page":"4333","DOI":"10.3168\/jds.2012-6269","article-title":"The relationship between endometrial cytology during estrous cycle and cutoff points for the diagnosis of subclinical endometritis in grazing dairy cows","volume":"96","author":"LV Madoz","year":"2013","journal-title":"J Dairy Sci"},{"issue":"9","key":"pone.0242874.ref033","doi-asserted-by":"crossref","first-page":"1879","DOI":"10.1016\/j.theriogenology.2005.04.022","article-title":"Prevalence of endometritis and its effects on reproductive performance of dairy cows","volume":"64","author":"RO Gilbert","year":"2005","journal-title":"Theriogenology"},{"issue":"6","key":"pone.0242874.ref034","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1530\/REP-14-0230","article-title":"Ovarian steroids do not affect bovine endometrial cytokine or chemokine responses to Escherichia coli or LPS in vitro","volume":"148","author":"JP Saut","year":"2014","journal-title":"Reproduction"},{"issue":"6","key":"pone.0242874.ref035","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1111\/j.1600-0897.2012.01106.x","article-title":"Explants of intact endometrium to model bovine innate immunity and inflammation ex vivo","volume":"67","author":"AM Borges","year":"2012","journal-title":"Am J Reprod Immunol"},{"key":"pone.0242874.ref036","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1186\/1471-2164-13-193","article-title":"RNA-seq analysis of differential gene expression in liver from lactating dairy cows divergent in negative energy balance","volume":"13","author":"M McCabe","year":"2012","journal-title":"BMC genomics"},{"key":"pone.0242874.ref037","doi-asserted-by":"crossref","first-page":"R86","DOI":"10.1186\/gb-2010-11-8-r86","article-title":"Galaxy: a comprehensive approach for supporting accessible, reproducible, and transparent computational research in the life sciences","volume":"11","author":"J Goecks","year":"2010","journal-title":"Genome Biol"},{"key":"pone.0242874.ref038","article-title":"Galaxy: a web-based genome analysis tool for experimentalists","author":"D Blankenberg","year":"2010","journal-title":"Curr Protoc Mol Biol"},{"issue":"10","key":"pone.0242874.ref039","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1101\/gr.4086505","article-title":"Galaxy: a platform for interactive large-scale genome analysis","volume":"15","author":"B Giardine","year":"2005","journal-title":"Genome Res"},{"issue":"15","key":"pone.0242874.ref040","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: a flexible trimmer for Illumina sequence data","volume":"30","author":"AM Bolger","year":"2014","journal-title":"Bioinformatics"},{"issue":"4","key":"pone.0242874.ref041","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast gapped-read alignment with Bowtie 2","volume":"9","author":"B Langmead","year":"2012","journal-title":"Nature methods"},{"issue":"3","key":"pone.0242874.ref042","doi-asserted-by":"crossref","first-page":"R25","DOI":"10.1186\/gb-2009-10-3-r25","article-title":"Ultrafast and memory-efficient alignment of short DNA sequences to the human genome","volume":"10","author":"B Langmead","year":"2009","journal-title":"Genome Biol"},{"issue":"7","key":"pone.0242874.ref043","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1093\/bioinformatics\/btt656","article-title":"featureCounts: an efficient general purpose program for assigning sequence reads to genomic features","volume":"30","author":"Y Liao","year":"2014","journal-title":"Bioinformatics"},{"issue":"12","key":"pone.0242874.ref044","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"MI Love","year":"2014","journal-title":"Genome Biol"},{"issue":"1","key":"pone.0242874.ref045","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1111\/j.2517-6161.1995.tb02031.x","article-title":"Controlling the false discovery rate: a practical and powerful approach to multiple testing","volume":"57","author":"Y Benjamini","year":"1995","journal-title":"J R Stat Soc"},{"issue":"1","key":"pone.0242874.ref046","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/nar\/28.1.27","article-title":"KEGG: Kyoto Encyclopedia of Genes and Genomes","volume":"28","author":"M Kanehisa","year":"2000","journal-title":"Nucleic Acids Res"},{"key":"pone.0242874.ref047","doi-asserted-by":"crossref","first-page":"D447","DOI":"10.1093\/nar\/gku1003","article-title":"STRING v10: protein-protein interaction networks, integrated over the tree of life","volume":"43","author":"D Szklarczyk","year":"2015","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"pone.0242874.ref048","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1186\/1866-1955-6-36","article-title":"PreImplantation factor (PIF*) promotes embryotrophic and neuroprotective decidual genes: effect negated by epidermal growth factor","volume":"6","author":"CM Duzyj","year":"2014","journal-title":"J Neurodev Disord"},{"key":"pone.0242874.ref049","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1186\/1477-7827-10-50","article-title":"PreImplantation Factor (PIF) promoting role in embryo implantation: increases endometrial Integrin-\u03b12\u03b23, amphiregulin and epiregulin while reducing betacellulin expression via MAPK in decidua","volume":"10","author":"ER Barnea","year":"2012","journal-title":"Reprod Biol Endocrin"},{"issue":"4","key":"pone.0242874.ref050","doi-asserted-by":"crossref","first-page":"3744","DOI":"10.3168\/jds.2018-15252","article-title":"Symposium review: Embryo survival-A genomic perspective of the other side of fertility","volume":"102","author":"H Khatib","year":"2019","journal-title":"J Dairy Sci"},{"issue":"4","key":"pone.0242874.ref051","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1095\/biolreprod.108.074336","article-title":"Progesterone-regulated changes in endometrial gene expression contribute to advanced conceptus development in cattle","volume":"81","author":"N Forde","year":"2009","journal-title":"Biol Reprod"},{"issue":"1","key":"pone.0242874.ref052","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1093\/biolre\/iox059","article-title":"The transcriptome of the endometrium and placenta is associated with pregnancy development but not lactation status in dairy cows","volume":"97","author":"SG Moore","year":"2017","journal-title":"Biol Reprod"},{"issue":"3","key":"pone.0242874.ref053","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1093\/biolre\/iox095","article-title":"Effect of metabolic status on conceptus-maternal interactions on day 19 in dairy cattle: II. Effects on the endometrial transcriptome","volume":"97","author":"S Bauersachs","year":"2017","journal-title":"Biol Reprod"},{"issue":"4","key":"pone.0242874.ref054","doi-asserted-by":"crossref","first-page":"930","DOI":"10.1111\/rda.13190","article-title":"Long-term effect of early nutrition on endocrine parameters and liver and endometrial gene expression of the members of the somatotrophic axis in Hereford heifers","volume":"53","author":"D Guggeri","year":"2018","journal-title":"Reprod Domest Anim"},{"issue":"3","key":"pone.0242874.ref055","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1111\/rda.12113","article-title":"Pre- and post-partum mild underfeeding influences gene expression in the reproductive tract of cyclic dairy cows","volume":"48","author":"D Valour","year":"2013","journal-title":"Reprod Domest Anim"},{"issue":"2","key":"pone.0242874.ref056","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1093\/molehr\/7.2.175","article-title":"The NF-\u03baB pathway in human endometrium and first trimester decidua","volume":"7","author":"AE King","year":"2001","journal-title":"Mol Human Reprod"},{"issue":"1","key":"pone.0242874.ref057","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.repbio.2017.01.003","article-title":"Global decrease in the expression of signalling pathways' genes in murine uterus during preimplantation pregnancy","volume":"17","author":"K Buska","year":"2017","journal-title":"Reprod Biol"},{"issue":"3","key":"pone.0242874.ref058","doi-asserted-by":"crossref","first-page":"e33625","DOI":"10.1371\/journal.pone.0033625","article-title":"Early developing pig embryos mediate their own environment in the maternal tract","volume":"7","author":"C Alminana","year":"2012","journal-title":"PLoS One"},{"issue":"6","key":"pone.0242874.ref059","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.1016\/j.theriogenology.2014.12.007","article-title":"Early embryonic and endometrial regulation of tumor necrosis factor and tumor necrosis factor receptor 2 in the cattle uterus","volume":"83","author":"E Correia-Alvarez","year":"2015","journal-title":"Theriogenology"},{"issue":"38","key":"pone.0242874.ref060","doi-asserted-by":"crossref","first-page":"13882","DOI":"10.1073\/pnas.1411674111","article-title":"PreImplantation factor promotes neuroprotection by targeting microRNA let-7","volume":"111","author":"M Mueller","year":"2014","journal-title":"PNAS USA"},{"issue":"7","key":"pone.0242874.ref061","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1016\/j.imbio.2016.02.004","article-title":"PreImplantation factor (PIF*) regulates systemic immunity and targets protective regulatory and cytoskeleton proteins","volume":"221","author":"ER Barnea","year":"2016","journal-title":"Immunobiology"},{"issue":"1","key":"pone.0242874.ref062","doi-asserted-by":"crossref","first-page":"S51","DOI":"10.1517\/14712598.2015.1044895","article-title":"Thymosin alpha1: burying secrets in the thymus","volume":"15","author":"S Moretti","year":"2015","journal-title":"Expert Opin Biol Ther"},{"key":"pone.0242874.ref063","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1749-6632.2012.06716.x","article-title":"Jack of all trades: thymosin alpha1 and its pleiotropy","volume":"1269","author":"L Romani","year":"2012","journal-title":"Ann N Y Acad Sci"},{"issue":"2","key":"pone.0242874.ref064","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1111\/cei.12264","article-title":"Toll-like receptors and CD40 modulate each other's expression affecting Leishmania major infection","volume":"176","author":"HS Chandel","year":"2014","journal-title":"Clin Exp Immunol"},{"issue":"3","key":"pone.0242874.ref065","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1042\/bst0310637","article-title":"Role of adapters in Toll-like receptor signalling","volume":"31","author":"S Akira","year":"2003","journal-title":"Biochem Soc 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Open"},{"issue":"6","key":"pone.0242874.ref079","first-page":"1287","article-title":"Chemokines and tissue injury","volume":"146","author":"MB Furie","year":"1995","journal-title":"Am J Pathol"},{"issue":"2","key":"pone.0242874.ref080","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.it.2003.12.005","article-title":"Chemokines: multiple levels of leukocyte migration control","volume":"25","author":"B Moser","year":"2004","journal-title":"Trends Immunol"},{"issue":"6","key":"pone.0242874.ref081","doi-asserted-by":"crossref","first-page":"1607","DOI":"10.1089\/ars.2010.3522","article-title":"Vascular cell adhesion molecule-1 expression and signaling during disease: regulation by reactive oxygen species and antioxidants","volume":"15","author":"JM Cook-Mills","year":"2011","journal-title":"Antioxid Redox Sign"},{"issue":"9","key":"pone.0242874.ref082","doi-asserted-by":"crossref","first-page":"5021","DOI":"10.4049\/jimmunol.1100646","article-title":"ICAM-1: getting a grip 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YAP\/TAZ Activity Mediates Leukocyte-Endothelial Adhesion by Regulating VCAM1 Expression in Endothelial Cells","volume":"19","author":"HJ Choi","year":"2018","journal-title":"Int J Mol Sci"},{"key":"pone.0242874.ref086","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.jprot.2018.08.011","article-title":"Integrated proteomic analysis of tumor necrosis factor alpha and interleukin 1beta-induced endothelial inflammation","volume":"192","author":"EP Beguin","year":"2019","journal-title":"J Proteomics"},{"issue":"5","key":"pone.0242874.ref087","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1071\/RD10230","article-title":"Immunological mechanisms to establish embryo tolerance in early bovine pregnancy","volume":"23","author":"AE Groebner","year":"2011","journal-title":"Reprod Fert Dev"},{"issue":"4","key":"pone.0242874.ref088","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1002\/vms3.126","article-title":"Preimplantation factor modulates acute 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